FER1L6 (NM_001039112) Human Tagged ORF Clone

CAT#: RC221305

FER1L6 (Myc-DDK-tagged)-Human fer-1-like 6 (C. elegans) (FER1L6)

Product Images

Circular map for RC221305

• 

  Western blot validation of overexpression lysate (Cat# LY422016) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221305 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified FER1L6 protein (Cat# TP321305). The protein was produced from HEK293T cells transfected with FER1L6 cDNA clone (Cat# RC221305) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FER1L6
• Synonyms: C8ORFK23
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221305 representing NM_001039112
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTGGGCTGAAGGTGAAGAAGAAGAGAAATAAGGCAGAGAAGGGGTTAATCCTAGCCAACAAGGCTG
  CGAAAGATAGTCAAGGTGACACTGAAGCACTGCAGGAGGAGCCTTCTCACCAGGAAGGACCGAGAGGAGA
  TTTGGTCCATGATGATGCTTCTATCTTTCCTGTCCCCTCAGCTTCTCCAAAGAGAAGATCAAAACTATTG
  ACTAAGATCCATGATGGGGAGGTCAGATCCCAAAATTATCAAATTGCCATAACCATCACCGAGGCTCGCC
  AGCTGGTGGGTGAGAACATTGACCCAGTTGTGACCATTGAGATTGGGGATGAGAAGAAGCAAAGCACAGT
  GAAGGAAGGAACCAACAGCCCATTTTATAATGAATACTTTGTCTTCGACTTCATTGGGCCCCAAGTGCAT
  CTTTTTGACAAGATCATCAAAATCTCCGTCTTTCACCACAAGCTGATAGGAAGTGTACTGATTGGCTCTT
  TCAAAGTAGACCTGGGGACCGTGTACAACCAACCTGGTCATCAGTTCTGCAACAAGTGGGCCCTGCTCAC
  AGACCCTGGTGACATCAGGACTGGCACCAAGGGGTACCTGAAATGTGACATCAGTGTCATGGGAAAAGGT
  GATGTCTTGAAGACCAGCCCTAAAACTTCTGACACCGAGGAGCCAATAGAAAAGAACCTTTTGATCCCCA
  ATGGGTTTCCACTGGAGAGACCGTGGGCCAGATTCTATGTGAGACTCTACAAAGCAGAAGGGTTGCCCAA
  AATGAATTCAAGCATCATGGCGAACGTCACCAAGGCATTTGTGGGTGACAGTAAGGACCTGGTGGATCCC
  TTTGTGGAGGTCTCCTTTGCTGGGCAGATGGGGCGAACCACAGTGCAGAAGAACTGTGCTGATCCTGTGT
  GGCATGAACAGGTGATCTTCAAGGAAATGTTCCCTCCCTTGTGTCGGAGGGTGAAAATCCAGGTGTGGGA
  TGAAGGCAGCATGAATGACGTAGCCCTGGCAACCCATTTCATTGACCTGAAGAAAATCTCCAACGAACAG
  GATGGAGACAAAGGCTTTCTGCCCACCTTTGGGCCTGCCTGGATTAACCTGTATGGCTCGCCCAGGAACC
  ACAGTCTGATGGATGACTACCAGGAAATGAACGAAGGCTTTGGGGAAGGTGTGTCATTCAGGGGCAGAAT
  CTTGGTAGAAATTGCTGTGGAAATCCTCTCAGGACGGGCACAGGAATCTAAATTTTCCAAGGCCCTGAAG
  GAGCTCAAGTTGCCTTCCAAGGACAAAGACTCCAAATCTTCCAAAGGTAAAGACAAGGCTGACAAAACTG
  AAGATGGAAAATCCCAACAGGCTTCAAACAAAACTAACTCAACCGAGGTGGAGGTGGAATCGTTCGATGT
  CCCCCCGGAGATTGTACCAGAAAAAAATGAGGAATTTTTACTCTTTGGAGCATTTTTTGAAGCTACCATG
  ATTGACCGGAAGATTGGAGATAAACCCATCAGCTTTGAAGTTTCTATTGGTAATTTTGGAAACCTGATTG
  ATGGAGGATCCCATTATGGGAGTAAGAAGTCAGCTGAATCAGCTGAAGAAGACCTCCTTCCACTGCTTCA
  CGAAGGGCAAGGGGATGTGGCCCATGATGTTCCCATTCCTATGGCCTCCACCACTCACCCGGAGAAGCCA
  CTGGTGACAGAAGGGAACAGGAATTACAACTATTTGCCATTTGAGGCTAAGAAGCCCTGTGTCTATTTCA
  TCAGCTCTTGGGGAGACCAGACCTTCAGGCTGCACTGGTCCAACATGCTGGAGAAAATGGCAGACTTCCT
  GGAAGAAAGTATAGAAGAAGTGAGAGAATTGATCAAGATTTCACAGGAGGCACCTGAAGAGAAAATGAAA
  ACAGTGCTCAGTGACTTCATCAGTCGGAGCAGTGCCTTTATCTCTGAAGCAGAAAAAAAGCCCAAGATGT
  TGAACCAAACCACTTTAGATAAGAAGCGACTTACGCTCTGCTGGCAGGAGCTGGAAGCAATGTGCAAGGA
  GGCCAAGGGGATCATTCAGCAGCAGAAGAAAAAGTTATCTGTTGATGAAATGATTCACGAAGCCCAAAAC
  TTTGTGGAAAAAATCCGCTTTCTTGTTGATGAGCCCCAGCACACTATCCCTGACGTTTTCATCTGGATGC
  TCAGCAACAACAGGAGAGTGGCCTATGCCCGCATCGCCTCCAAAGACCTCCTCTATTCCCCTGTCGCGGG
  GCAGATGGGCAAACACTGCGGCAAGATCAAAACTCACTTCCTCAAACCTCCTGGGAAACGACCGGCTGGT
  TGGTCTGTGCAAGCAAAAGTCGACGTGTACCTGTGGCTGGGCTCCATCAAGCATGCCAGTGCCATTTTGG
  ACAACTTGCCAGTAGGCTATGAAGCAGAAATGTCCTCCAAAGGGGCTGGCACCAATCACCCCCCATCTAA
  CCTGCTCTACCAAGAACAGCATGTTTTTCAGCTGAGGGCTCACATGTACCAAGCCCGGGGCCTCATCGCA
  GCTGACAGCAATGGACTTTCAGACCCTTTTGCCAAAGTCACGTTCCTTTCTCACTGCCAGACAACAAAGA
  TAATCTCCCAGACCCTCTCTCCGACCTGGAACCAGATGCTGCTGTTCAATGATTTGGTGCTGCATGGAGA
  TGTGAAGGAGCTGGCAGAGTCCCCGCCCTTAGTGGTGGTGGAGCTGTATGACAGCGACGCTGTGGGGAAG
  CCAGAATATTTGGGTGCCACAGTGGCTGCTCCTGTTGTGAAGCTGGCTGACCAGGACTATGAGCCCCCCA
  GGTTATGCTATCACCCCATCTTTTGTGGGAATCTCTCTGGAGGGGATCTCCTTGCTGTATTTGAACTGCT
  GCAGGTTCCTCCTTCTGGGCTGCAAGGCCTCCCACCCGTTGAGCCACCAGACATCACCCAGATCTACCCG
  GTTCCTGCCAACATTCGGCCGGTGCTGAGCAAATACCGAGTGGAGGTTCTCTTCTGGGGAGTTCGGGAAA
  TGAAGAAGGTGCAGCTCCTCTCTGTGGATCGGCCTCAGGCTCTCATTGAGTGCGGAGGACAAGGTGTGAA
  GTCCTGCGTGATCCAGAGCTACAAGAACAACCCGAACTTCAGCATCCAGGCAGACGCTTTCGAAGTGGAA
  CTGCCTGAGAACGAGCTTCTGCACCCGCCACTGAGCATCTGCGTGGTGGACTGGAGAGCTTTTGGGAGGA
  GTACCCTTGTGGGCACCTACACCATCAACTACTTGAAGCAGTTTTTGTGTAAACTCAGAGAGCCCCTTGC
  CCCCATCACACAGGTGGATGGAACCCAGCCTGGGCACGATATTTCAGATTCGCTAACAGCCACTGAGTCC
  TCTGGAGCCCACAGCTCCTCCCAGGATCCCCCAGCAGATCACATTTATGTGGATGTTGAGCCACCTCCCA
  CAGTGGTGCCCGACTCTGCCCAGGCCCAGCCGGCCATCCTGGTTGACGTCCCTGACTCATCCCCGATGCT
  GGAGCCTGAACACACACCTGTAGCCCAGGAGCCACCAAAAGATGGAAAACCTAAGGATCCCAGGAAGCCT
  TCCCGGAGGTCCACTAAGAGGAGAAAGAGGACCATAGCAGATGAATCTGCTGAAAACGTGATTGACTGGT
  GGTCTAAGTATTATGCCTCCCTGAAGAAAGCCCAGAAGGCAAAGGAGAGAAATCCCAAGGGAAAAAAAGG
  CAATACAGAGGCAAAGCCAGATGAGGTAGTGGTAGATATAGAAGATGGGCCAAAGAAGAAGAAAGACAAA
  ATGCTCAAGAAGAAACCCAAAGATGATGGAATCCCCAACCTGGCCATCTTGCAGATATATGACGGTGATC
  TCGAGAGTGAATTCAACAATTTTGAAGACTGGGTGAAAACTTTTGAGCTCTTCAGAGGCAAGTCTACGGA
  AGATGACCATGGTCTTGATGGAGACCNAGTCATAGGAAAATTTAAGGGCTCCTTCTGCATCTACAAAAGC
  CCCCAGGATTCTAGCTCTGAGGACAGCGGGCAGCTGAGAATCCAGCAAGGGATTCCGCCCAATCACCCTG
  TCACAGTGCTGATCAGAGTATACATTGTCGCGGCATTTAATCTTAGTCCAGCTGATCCAGATGGCAAATC
  AGATCCCTACATTGTGATCAAGCTTGGCAAGACAGAAATCAAAGACCGGGATAAATACATCCCTAAACAA
  CTGAACCCAGTATTTGGAAGGTCATTTGAGATCCAAGCCACATTCCCAAAAGAGTCCCTGCTCTCCATCC
  TGATCTATGACCATGACATGATTGGCACAGATGACCTTATTGGTGAGACCAAGATCGACCTGGAGAACCG
  CTTCTACAGCAAACACCGAGCCATCTGTGGCTTGCAGAGCCAGTATGAGATAGAAGGATACAATGCCTGG
  AGAGACACGTCCAAACCCACCGAAATCCTCACTAAGCTCTGCAAAGACAACAAGCTGGATGGACCCTACT
  TTCACCCTGGGAAAATACAGATAGGAAACCAAGTCTTTTCTGGAAAAACTATCTTCACTGAAGAGGACAC
  TGATGAGACAGTGGAGTCTTATGAACACCTGGCCCTCAAGGTTTTACACTCTTGGGAGGATATCCCAGAA
  GTTGGGTGTAGGCTGGTTCCTGAACACATAGAAACTCGGCCACTGTACCACAAGGATAAGCCAGGAATGG
  AGCAGGGCCGCCTGCAGATGTGGGTGGACATGTTTCCCAAGGATATGCCTCAACCTGGACCTCCTGTTGA
  CATCTCTCCAAGGCGACCCAAAGGATACGAATTGAGAGTGACCATCTGGAACACTGAAGATGTCATTTTA
  GAGGATGAGAATATCTTCACAGGCCAAAAATCAAGTGATATTTATGTGAAAGGGTGGTTAAAGGGCTTGG
  AGGATGACAAGCAGGAGACAGATGTGCATTACAACTCCCTGACTGGAGAGGGCAACTTCAACTGGCGCTT
  CCTGTTTCCCTTTCAGTATCTCCCAGCTGAGAAGCAAATGGTCATTACCAAGAGGGAGAACATCTTCTCT
  TTAGAGAAGATGGAGTGTAAGACTCCTGCTGTGTTGGTGCTGCAGGTTTGGGATTTTGAAAGGCTGTCCT
  CAGATGACTTCCTGGGCACCCTGGAAATGAACCTCAACAGTTTCCCTCGAGCAGCTAAGTCTGCCAAAGC
  CTGTGATCTTGCCAAGTTTGAAAATGCAAGTGAGGAGACCAAGATCTCTATATTCCAGCAAAAACGTGTG
  CGTGGCTGGTGGCCTTTTTCTAAAAGCAAAGAACTCACAGGCAAGGTTGAAGCTGAGTTCCACCTAGTTA
  CAGCAGAAGAAGCTGAGAAAAATCCTGTTGGAAAAGCCCGAAAGGAGCCAGAGCCCCTGGCCAAGCCCAA
  CCGCCCAGACACCTCCTTTTCGTGGTTCATGAGCCCCTTTAAGTGCCTGTACTACCTCATCTGGAAGAAT
  TACAAAAAGTACATCATCATTGCTTTCATTCTCATCATCCTCATCATCTTCCTCGTCCTTTTCATCTACA
  CCTTGCCAGGAGCCATCAGCCGAAGGATCGTTGTGGGCTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221305 representing NM_001039112
  Red=Cloning site Green=Tags(s)
  
  MFGLKVKKKRNKAEKGLILANKAAKDSQGDTEALQEEPSHQEGPRGDLVHDDASIFPVPSASPKRRSKLL
  TKIHDGEVRSQNYQIAITITEARQLVGENIDPVVTIEIGDEKKQSTVKEGTNSPFYNEYFVFDFIGPQVH
  LFDKIIKISVFHHKLIGSVLIGSFKVDLGTVYNQPGHQFCNKWALLTDPGDIRTGTKGYLKCDISVMGKG
  DVLKTSPKTSDTEEPIEKNLLIPNGFPLERPWARFYVRLYKAEGLPKMNSSIMANVTKAFVGDSKDLVDP
  FVEVSFAGQMGRTTVQKNCADPVWHEQVIFKEMFPPLCRRVKIQVWDEGSMNDVALATHFIDLKKISNEQ
  DGDKGFLPTFGPAWINLYGSPRNHSLMDDYQEMNEGFGEGVSFRGRILVEIAVEILSGRAQESKFSKALK
  ELKLPSKDKDSKSSKGKDKADKTEDGKSQQASNKTNSTEVEVESFDVPPEIVPEKNEEFLLFGAFFEATM
  IDRKIGDKPISFEVSIGNFGNLIDGGSHYGSKKSAESAEEDLLPLLHEGQGDVAHDVPIPMASTTHPEKP
  LVTEGNRNYNYLPFEAKKPCVYFISSWGDQTFRLHWSNMLEKMADFLEESIEEVRELIKISQEAPEEKMK
  TVLSDFISRSSAFISEAEKKPKMLNQTTLDKKRLTLCWQELEAMCKEAKGIIQQQKKKLSVDEMIHEAQN
  FVEKIRFLVDEPQHTIPDVFIWMLSNNRRVAYARIASKDLLYSPVAGQMGKHCGKIKTHFLKPPGKRPAG
  WSVQAKVDVYLWLGSIKHASAILDNLPVGYEAEMSSKGAGTNHPPSNLLYQEQHVFQLRAHMYQARGLIA
  ADSNGLSDPFAKVTFLSHCQTTKIISQTLSPTWNQMLLFNDLVLHGDVKELAESPPLVVVELYDSDAVGK
  PEYLGATVAAPVVKLADQDYEPPRLCYHPIFCGNLSGGDLLAVFELLQVPPSGLQGLPPVEPPDITQIYP
  VPANIRPVLSKYRVEVLFWGVREMKKVQLLSVDRPQALIECGGQGVKSCVIQSYKNNPNFSIQADAFEVE
  LPENELLHPPLSICVVDWRAFGRSTLVGTYTINYLKQFLCKLREPLAPITQVDGTQPGHDISDSLTATES
  SGAHSSSQDPPADHIYVDVEPPPTVVPDSAQAQPAILVDVPDSSPMLEPEHTPVAQEPPKDGKPKDPRKP
  SRRSTKRRKRTIADESAENVIDWWSKYYASLKKAQKAKERNPKGKKGNTEAKPDEVVVDIEDGPKKKKDK
  MLKKKPKDDGIPNLAILQIYDGDLESEFNNFEDWVKTFELFRGKSTEDDHGLDGDXVIGKFKGSFCIYKS
  PQDSSSEDSGQLRIQQGIPPNHPVTVLIRVYIVAAFNLSPADPDGKSDPYIVIKLGKTEIKDRDKYIPKQ
  LNPVFGRSFEIQATFPKESLLSILIYDHDMIGTDDLIGETKIDLENRFYSKHRAICGLQSQYEIEGYNAW
  RDTSKPTEILTKLCKDNKLDGPYFHPGKIQIGNQVFSGKTIFTEEDTDETVESYEHLALKVLHSWEDIPE
  VGCRLVPEHIETRPLYHKDKPGMEQGRLQMWVDMFPKDMPQPGPPVDISPRRPKGYELRVTIWNTEDVIL
  EDENIFTGQKSSDIYVKGWLKGLEDDKQETDVHYNSLTGEGNFNWRFLFPFQYLPAEKQMVITKRENIFS
  LEKMECKTPAVLVLQVWDFERLSSDDFLGTLEMNLNSFPRAAKSAKACDLAKFENASEETKISIFQQKRV
  RGWWPFSKSKELTGKVEAEFHLVTAEEAEKNPVGKARKEPEPLAKPNRPDTSFSWFMSPFKCLYYLIWKN
  YKKYIIIAFILIILIIFLVLFIYTLPGAISRRIVVGS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001039112
• ORF Size: 5571 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039112.1, NP_001034201.1
• RefSeq Size: 6058 bp
• RefSeq ORF: 5574 bp
• Locus ID: 654463
• UniProt ID: Q2WGJ9
• Cytogenetics: 8q24.13
• Protein Families: Transmembrane
• MW: 209.1 kDa