OTOA (NM_144672) Human Tagged ORF Clone

CAT#: RC221145

OTOA (Myc-DDK-tagged)-Human otoancorin (OTOA), transcript variant 1

Product Images

Circular map for RC221145

• 

  Western blot validation of overexpression lysate (Cat# LY408211) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221145 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified OTOA protein (Cat# TP321145). The protein was produced from HEK293T cells transfected with OTOA cDNA clone (Cat# RC221145) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: OTOA
• Synonyms: CT108; DFNB22
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221145 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCAGGAACCTACGACATACTCCCTTTTCCTATTCCTTTTTCTGAGCCATGGAGTGTCGAGTTATA
  CAGTGCCAAATTCCAGGCAGGATTTGCATCCATTGTTGCAAAACATGGCGGAAGAAATAATAGATGGAAG
  CTATCTGAATGCACTGCTGGATCTCATACAGTTTCAAAGCTCCCACGTGTGGACGGATGACCTGTCCCAC
  AGAGTCCTGGCCTATCTGAATTCCCGGAATGTTGCCTTCACCATCCCCAGCCTGCAGGCAGCCGTGGAAA
  ACCACCTGGAGCAGCGTCTGCACCAGCCCCAGAAGCTGCTGGAGGACCTGAGGAAGACAGACGCCCAGCA
  GTTCCGCACTGCCATGAAATGCCTCTTAGAAGACAAGAAGGACGGCTTGGACCTGAAAGACATCATCATC
  GACTTAGGAGAGATTCGAGAACGAGCCTTGCAGAGCCCTGGCGTGAACCGCAGCCTGTTTCTCATCACAC
  TGGAGAGGTGTTTCCAGATGCTGAACTCCCTGGAGTGTGTGGAGATCCTGGGCAAGGTGCTGAGGGGGTC
  CTCAGGGAGCTTTCTCCAGCCAGACATCACAGAGCGGCTCCCTCGGGACCTGCGCGAGGATGCCTTTAAG
  AACCTATCTGCAGTGTTCAAAGATCTCTACGACAAAACCTCGGCTCATTCCCAGAGAGCTCTCTATTCCT
  GGATGACTGGAATACTGCAGACATCCTCCAATGCCACTGATGACTCTGCTTCATGGGTCAGTGCGGAACA
  CTTATGGGTTTTGGGCAGATACATGGTTCACCTATCGTTTGAAGAAATTACGAAAATTAGTCCTATAGAA
  ATTGGGCTGTTTATCAGCTATGACAACGCCACCAAGCAGCTGGACATGGTCTATGACATCACACCTGAGC
  TGGCCCAGGCGTTTCTGGAGAGGATCAGCTCCTCCAACTTTAACATGAGGAATACCTCCACCATCCACAG
  GCTGGGGCTGCTGGTTTGTTTCTACAATGACCTGGAATTGCTGGATGCCACTGTGGCTCAAGTCCTGCTT
  TACCAGATGATCAAGTGCAGCCACCTGAGGGGCTTCCAGGCTGGCGTCCAGAAGCTCAAAGCAGAACTCC
  TGGACATTGCCATGGAGAACCAGACCCTCAATGAGACCCTGGGTTCTTTGTCGGATGCAGTTGTAGGTTT
  GACCTACAGCCAACTGGAATCCCTCTCCCCCGAGGCTGTGCACGGAGCCATCTCCACCCTCAACCAGGTC
  TCAGGTTGGGCCAAGAGCCAGGTCATCATCTTGTCTGCCAAATACTTGGCCCATGAGAAGGTGCTGTCTT
  TCTACAATGTCAGCCAGATGGGCGCACTGCTGGCTGGGGTCAGCACCCAGGCCTTCTGCAGCATGAAACG
  CAAGGACATCTCGCAGGTCCTGAGAAGTGCCGTCTCCCAGTATGTATCCGACTTGTCACCTGCCCAGCAG
  CAAGGTATCCTCAGCAAGATGGTCCAAGCGGAAGACACTGCCCCAGGCATCGTGGAGATACAAGGGGCTT
  TCTTTAAGGAAGTGTCTCTCTTTGATTTAAGGAGGCAACCTGGATTCAACTCTACAGTCCTGAAGGATAA
  GGAACTTGGAAGGAGCCAGGCTCTGTTCCTGTATGAGCTTCTGTTAAAGACCACCAGAAGGCCTGAGGAG
  CTTTTGAGTGCTGGGCAGCTGGTCAAAGGCGTGACCTGCTCACACATTGATGCCATGAGCACTGACTTCT
  TTCTGGCCCATTTCCAGGATTTTCAGAACAACTTCGCCCTGCTTTCACCCTATCAGGTTAATTGTTTGGC
  GTGGAAATACTGGGAAGTTTCCAGATTGTCTATGCCACCTTTCCTCTTGGCTGCACTCCCGGCCCGCTAC
  CTGGCTTCTGTCCCAGCCTCCCAGTGTGTGCCCTTTCTGATCAGCCTGGGGAAGAGCTGGTTGGACTCCT
  TGGTTTTAGATTCCCACAAAAAGACTTCAGTCCTCAGGAAAGTGCAGCAGTGCCTGGACGACTCCATTGC
  TGATGAGTACACTGTGGACATCATGGGGAACCTGCTGTGTCACTTGCCGGCAGCCATCATCGACAGGGGG
  ATCTCCCCCAGGGCTTGGGCGACTGCTCTACACGGCCTCAGAGACTGCCCAGACCTCAACCCTGAGCAAA
  AGGCTGCAGTGAGGCTCAAGCTCCTGGGACAGTATGGACTCCCTCAGCACTGGACAGCCGAGACCACGAA
  GGACTTGGGACCCTTTCTAGTACTTTTCTCAGGAGATGAATTAAGCTCTATAGCCACAAAGTTTCCTGAG
  ATCCTTCTGCAAGCAGCTTCCAAGATGGCCAGGACCCTGCCCACTAAAGAATTCCTCTGGGCTGTCTTTC
  AGTCTGTTCGGAACAGCAGTGATAAGATCCCCAGCTATGACCCTATGCCTGGTTGCCATGGAGTCGTGGC
  CCCCTCTTCTGATGACATCTTCAAGTTGGCCGAAGCCAACGCCTGCTGGGCCCTGGAGGACCTGCGGTGC
  ATGGAGGAAGACACATTCATCAGGACCGTGGAACTGCTGGGAGCTGTCCAGGGTTTCAGCCGGCCTCAGC
  TGATGACCCTGAAGGAGAAAGCAATACAGGTTTGGGACATGCCATCTTACTGGAGAGAACACCATATCGT
  CTCCCTGGGGCGCATTGCTCTGGCTCTTAATGAGAGTGAGCTGGAGCAGCTGGACCTCAGCTCCATAGAC
  ACTGTGGCTTCCCTAAGCTGGCAAACAGAATGGACCCCGGGACAGGCTGAATCCATTTTGCAAGGGTACC
  TGGATGATTCAGGATACAGTATCCAGGACCTGAAGAGCTTTCATTTGGTAGGACTTGGTGCAACCCTGTG
  TGCTATAAACATCACTGAAATCCCACTTATAAAGATCTCAGAATTCAGGGTGGTAGTGGCCAGAATTGGG
  ACCCTGCTCTGCAGCACACATGTCTTAGCCGAGTTTAAGAGGAAGGCTGAAGTTGTGTTTGGGGATCCCA
  CTGAGTGGACCAGTTCTGTCTTGCAGGAGCTTGGGACCATTGCAGCTGGATTAACTAAGGCAGCGCTCCG
  GATGCTTGACAAGGATTTGATGCCATATTTCCAGCCATCAGCAATAAAATGCCTTCCTGATGAGATATTC
  AAAGAGCTGTCCGCGGAGCAGATCGCCTCCCTGGGTCCGGAGAACGCCGCGGCGGTGACCCACGCCCAGC
  GCCGGCGGCTCAGTCCACTGCAGCTGCAGAGCCTCCAGCAGGCGCTAGATGGCGCCAAGACTCACTCCTG
  GCAGGACGCGCCCGCTAGCGCCGGTCCCACTAGAACCTCATCCTCGCGTTCTCCCGCAGGAGCTCTCCAG
  TCGTGGGGTCTTTGGCTTGGTTGTCCCCTGCTGGTTCTAATGGCCAAGCTCCTGTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221145 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSQEPTTYSLFLFLFLSHGVSSYTVPNSRQDLHPLLQNMAEEIIDGSYLNALLDLIQFQSSHVWTDDLSH
  RVLAYLNSRNVAFTIPSLQAAVENHLEQRLHQPQKLLEDLRKTDAQQFRTAMKCLLEDKKDGLDLKDIII
  DLGEIRERALQSPGVNRSLFLITLERCFQMLNSLECVEILGKVLRGSSGSFLQPDITERLPRDLREDAFK
  NLSAVFKDLYDKTSAHSQRALYSWMTGILQTSSNATDDSASWVSAEHLWVLGRYMVHLSFEEITKISPIE
  IGLFISYDNATKQLDMVYDITPELAQAFLERISSSNFNMRNTSTIHRLGLLVCFYNDLELLDATVAQVLL
  YQMIKCSHLRGFQAGVQKLKAELLDIAMENQTLNETLGSLSDAVVGLTYSQLESLSPEAVHGAISTLNQV
  SGWAKSQVIILSAKYLAHEKVLSFYNVSQMGALLAGVSTQAFCSMKRKDISQVLRSAVSQYVSDLSPAQQ
  QGILSKMVQAEDTAPGIVEIQGAFFKEVSLFDLRRQPGFNSTVLKDKELGRSQALFLYELLLKTTRRPEE
  LLSAGQLVKGVTCSHIDAMSTDFFLAHFQDFQNNFALLSPYQVNCLAWKYWEVSRLSMPPFLLAALPARY
  LASVPASQCVPFLISLGKSWLDSLVLDSHKKTSVLRKVQQCLDDSIADEYTVDIMGNLLCHLPAAIIDRG
  ISPRAWATALHGLRDCPDLNPEQKAAVRLKLLGQYGLPQHWTAETTKDLGPFLVLFSGDELSSIATKFPE
  ILLQAASKMARTLPTKEFLWAVFQSVRNSSDKIPSYDPMPGCHGVVAPSSDDIFKLAEANACWALEDLRC
  MEEDTFIRTVELLGAVQGFSRPQLMTLKEKAIQVWDMPSYWREHHIVSLGRIALALNESELEQLDLSSID
  TVASLSWQTEWTPGQAESILQGYLDDSGYSIQDLKSFHLVGLGATLCAINITEIPLIKISEFRVVVARIG
  TLLCSTHVLAEFKRKAEVVFGDPTEWTSSVLQELGTIAAGLTKAALRMLDKDLMPYFQPSAIKCLPDEIF
  KELSAEQIASLGPENAAAVTHAQRRRLSPLQLQSLQQALDGAKTHSWQDAPASAGPTRTSSSRSPAGALQ
  SWGLWLGCPLLVLMAKLLW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144672
• ORF Size: 3417 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144672.3, NP_653273.3
• RefSeq Size: 3624 bp
• RefSeq ORF: 3420 bp
• Locus ID: 146183
• Cytogenetics: 16p12.2
• MW: 126.8 kDa
• Gene Summary: The protein encoded by this gene is specifically expressed in the inner ear, and is located at the interface between the apical surface of the inner ear sensory epithelia and their overlying acellular gels. It is prposed that this protein is involved in the attachment of the inner ear acellular gels to the apical surface of the underlying nonsensory cells. Mutations in this gene are associated with autosomal recessive deafness type 22 (DFNB22). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]