LIMCH1 (NM_014988) Human Tagged ORF Clone

CAT#: RC221099

LIMCH1 (Myc-DDK-tagged)-Human LIM and calponin homology domains 1 (LIMCH1), transcript variant 1

Product Images

Circular map for RC221099

• 

  Western blot validation of overexpression lysate (Cat# LY414882) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221099 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified LIMCH1 protein (Cat# TP321099). The protein was produced from HEK293T cells transfected with LIMCH1 cDNA clone (Cat# RC221099) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LIMCH1
• Synonyms: LIMCH1A; LMO7B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221099 representing NM_014988
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTGTCCCGCTCTCGGTCTGGAAGCTCTTCAGCCCCTGCAGCCCGAGCCGCCCCCCGAGCCCGCCT
  TCTCCGAGGCGCAGAAGTGGATTGAGCAAGTAACTGGCAGAAGTTTTGGTGATAAAGATTTTCGGACAGG
  TTTAGAAAATGGAATCCTCCTCTGCGAGTTGCTGAATGCTATAAAGCCAGGACTTGTTAAAAAGATCAAT
  AGATTGCCTACCCCCATTGCAGGACTGGACAATATTATCTTATTCTTGAGAGGTTGTAAAGAGCTCGGCC
  TTAAAGAATCTCAACTTTTTGACCCGAGTGACCTCCAGGATACATCCAACAGAGTAACAGTCAAGAGCCT
  TGATTATAGTAGGAAGCTGAAAAATGTATTAGTTACCATTTACTGGCTGGGAAAAGCAGCAAACAGCTGC
  ACATCCTACAGCGGAACGACACTAAACCTGAAGGAGTTTGAAGGATTGTTGGCTCAGATGCGAAAGGACA
  CTGATGACATTGAAAGTCCTAAACGCAGTATCCGAGACAGTGGCTACATCGACTGCTGGGATTCCGAGCG
  CAGCGACTCCCTCTCTCCTCCTCGCCACGGCAGAGATGATTCCTTCGACAGCCTGGATTCCTTTGGCTCT
  CGCTCTCGGCAGACGCCTTCACCAGATGTAGTCCTCAGGGGAAGCAGCGATGGGAGAGGAAGCGACTCTG
  AATCCGACTTGCCTCATCGGAAGCTGCCAGATGTGAAGAAGGATGACATGTCTGCACGGCGGACTTCCCA
  TGGTGAGCCGAAATCAGCAGTGCCTTTTAACCAGTACCTCCCGAACAAAAGCAATCAGACGGCCTACGTC
  CCCGCGCCTCTGAGAAAGAAGAAAGCAGAGAGAGAGGAATACCGCAAGAGCTGGAGTACCGCCACCTCCC
  CGCTGGGTGGGGAGAGGCCCTTCAGATACGGTCCGAGAACTCCTGTGTCTGATGACGCAGAGAGCACCAG
  CATGTTTGACATGCGGTGTGAGGAGGAGGCCGCGGTGCAGCCGCACAGCAGGGCCCGCCAGGAGCAGCTG
  CAGCTGATAAATAACCAGCTGAGGGAAGAGGACGACAAATGGCAAGATGACCTGGCTCGTTGGAAGAGTC
  GTAGAAGAAGTGTTTCTCAGGACTTAATCAAGAAAGAGGAAGAAAGGAAAAAAATGGAGAAGTTACTGGC
  TGGAGAAGATGGGACAAGTGAACGAAGGAAAAGCATCAAAACCTACAGAGAAATTGTTCAAGAAAAAGAG
  CGGAGAGAGAGAGAGCTGCATGAAGCATATAAGAACGCTCGGTCCCAGGAGGAGGCAGAGGGGATCCTTC
  AACAGTACATTGAGAGGTTCACCATCAGTGAGGCTGTTCTCGAACGCTTGGAGATGCCAAAAATTCTGGA
  AAGAAGCCATTCAACAGAGCCAAATTTATCCTCCTTCCTGAATGACCCCAATCCCATGAAATACCTGCGG
  CAACAGTCACTGCCTCCACCCAAATTCACTGCCACTGTTGAAACCACCATTGCTCGTGCCAGTGTTCTGG
  ATACCAGCATGTCAGCAGGCAGTGGGTCTCCAAGCAAAACTGTCACTCCCAAAGCAGTGCCTATGCTGAC
  ACCCAAGCCTTACTCCCAGCCCAAAAATTCTCAAGATGTTCTGAAGACCTTTAAGGTAGACGGGAAAGTC
  AGTGTGAATGGAGAGACGGTTCATAGAGAGGAGGAGAAGGAAAGAGAGTGTCCCACGGTGGCACCTGCCC
  ACTCCTTAACCAAATCCCAGATGTTTGAAGGTGTGGCCAGAGTGCACGGGTCTCCACTGGAGCTGAAACA
  AGACAACGGTAGCATCGAGATCAACATAAAGAAGCCAAACTCTGTTCCCCAAGAGCTCGCAGCAACCACT
  GAGAAAACGGAACCGAATAGTCAAGAGGACAAGAATGATGGTGGAAAATCAAGAAAAGGGAATATAGAAC
  TTGCCTCATCAGAACCACAGCATTTTACAACAACTGTGACTCGATGCAGCCCGACCGTGGCCTTTGTGGA
  ATTTCCCTCCAGCCCCCAGCTGAAGAATGATGTGTCGGAAGAAAAAGACCAGAAGAAACCAGAAAATGAA
  ATGAGTGGAAAGGTGGAGTTGGTGCTGTCACAAAAGGTGGTAAAGCCAAAATCTCCAGAACCCGAAGCAA
  CGCTGACATTTCCATTTCTGGACAAAATGCCTGAAGCCAACCAACTACATTTGCCAAATCTCAATTCTCA
  AGTGGATTCTCCAAGCAGTGAGAAGTCACCTGTTACGACACCTCAGTTTAAGTTCTGGGCATGGGACCCA
  GAAGAGGAGCGCAGGCGACAGGAAAAATGGCAACAGGAACAGGAACGTTTGCTCCAGGAGAGATACCAGA
  AGGAGCAGGACAAGCTGAAAGAAGAGTGGGAAAAGGCCCAAAAGGAGGTGGAAGAGGAAGAACGCAGATA
  CTATGAGGAGGAGCGTAAGATAATTGAAGACACTGTGGTTCCATTTACTGTTTCTTCAAGTTCCGCTGAC
  CAGCTGTCTACCTCTTCCTCCATGACTGAAGGCAGTGGGACAATGAATAAGATAGACCTGGGAAACTGTC
  AAGATGAAAAACAAGACAGAAGATGGAAGAAATCATTCCAGGGAGATGACAGTGACTTATTGCTGAAGAC
  TAGGGAAAGTGATCGACTGGAGGAGAAGGGCAGCCTAACTGAAGGGGCCTTGGCTCATTCTGGGAACCCT
  GTATCAAAAGGAGTCCATGAAGACCATCAGCTGGATACCGAGGCTGGGGCCCCACACTGTGGAACAAACC
  CACAGCTTGCTCAGGATCCATCCCAGAATCAGCAGACATCAAATCCAACGCACAGTTCAGAAGATGTGAA
  GCCAAAAACCCTCCCGCTGGATAAAAGCATTAACCATCAGATCGAGTCTCCCAGTGAAAGGCGGAAGAAA
  AGTCCCCGAGAGCACTTCCAGGCTGGGCCTTTCTCTCCCTGTTCTCCCACCCCTCCCGGTCAGTCACCAA
  ACAGGTCTATAAGTGGAAAGAAGCTGTGCTCTTCCTGTGGGCTTCCTTTGGGTAAAGGAGCTGCAATGAT
  CATCGAGACCCTCAATCTCTATTTTCACATCCAGTGTTTCAGGTGTGGAATTTGTAAAGGCCAGCTTGGA
  GATGCAGTGAGTGGGACGGATGTTAGGATTCGAAATGGTCTCCTGAACTGTAATGATTGCTACATGCGAT
  CCAGAAGTGCCGGGCAGCCTACAACATTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221099 representing NM_014988
  Red=Cloning site Green=Tags(s)
  
  MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKIN
  RLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAANSC
  TSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGS
  RSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQTAYV
  PAPLRKKKAEREEYRKSWSTATSPLGGERPFRYGPRTPVSDDAESTSMFDMRCEEEAAVQPHSRARQEQL
  QLINNQLREEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKE
  RRERELHEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLR
  QQSLPPPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKV
  SVNGETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATT
  EKTEPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENE
  MSGKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQVDSPSSEKSPVTTPQFKFWAWDP
  EEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTVSSSSAD
  QLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLLKTRESDRLEEKGSLTEGALAHSGNP
  VSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSEDVKPKTLPLDKSINHQIESPSERRKK
  SPREHFQAGPFSPCSPTPPGQSPNRSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQLG
  DAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014988
• ORF Size: 3249 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014988.1, NP_055803.1
• RefSeq Size: 5113 bp
• RefSeq ORF: 3252 bp
• Locus ID: 22998
• UniProt ID: Q9UPQ0
• Cytogenetics: 4p13
• MW: 121.7 kDa
• Gene Summary: Actin stress fibers-associated protein that activates non-muscle myosin IIa. Activates the non-muscle myosin IIa complex by promoting the phosphorylation of its regulatory subunit MRLC/MYL9. Through the activation of non-muscle myosin IIa, positively regulates actin stress fibers assembly and stabilizes focal adhesions. It therefore negatively regulates cell spreading and cell migration.[UniProtKB/Swiss-Prot Function]