BTG3 (NM_006806) Human Tagged ORF Clone

CAT#: RC221056

BTG3 (Myc-DDK-tagged)-Human BTG family, member 3 (BTG3), transcript variant 2

Product Images

Circular map for RC221056

• 

  Western blot validation of overexpression lysate (Cat# LY416410) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221056 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BTG3
• Synonyms: ANA; ANA/BTG3; APRO4; TOB5; TOB55; TOFA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221056 representing NM_006806
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAATGAAATTGCTGCCGTTGTCTTCTTTTTCACAAGGCTAGTTCGAAAACATGATAAGTTGAAAA
  AAGAGGCAGTTGAGAGGTTTGCTGAGAAATTGACCCTAATACTTCAAGAAAAATATAAAAATCACTGGTA
  TCCAGAAAAACCATCGAAAGGACAGGCCTACAGATGTATTCGTGTCAATAAATTTCAGAGAGTTGATCCT
  GATGTCCTGAAAGCCTGTGAAAACAGCTGCATCTTGTATAGTGACCTGGGCTTGCCAAAGGAGCTCACTC
  TCTGGGTGGACCCATGTGAGGTGTGCTGTCGGTATGGAGAGAAAAACAATGCATTCATTGTTGCCAGCTT
  TGAAAATAAAGATGAGAACAAGGATGAGATCTCCAGGAAAGTTACCAGGGCCCTTGATAAGGTTACCTCT
  GATTATCATTCAGGATCCTCTTCTTCAGATGAAGAAACAAGTAAGGAAATGGAAGTGAAACCCAGTTCGG
  TGACTGCAGCCGCAAGTCCTGTGTACCAGATTTCAGAACTTATATTTCCACCTCTTCCAATGTGGCACCC
  TTTGCCCAGAAAAAAGCCAGGAATGTATCGAGGGAATGGCCATCAGAATCACTATCCTCCTCCTGTTCCA
  TTTGGTTATCCAAATCAGGGAAGAAAAAATAAACCATATCGCCCAATTCCAGTGACATGGGTACCTCCTC
  CTGGAATGCATTGTGACCGGAATCACTGGATTAATCCTCACATGTTAGCACCTCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221056 representing NM_006806
  Red=Cloning site Green=Tags(s)
  
  MKNEIAAVVFFFTRLVRKHDKLKKEAVERFAEKLTLILQEKYKNHWYPEKPSKGQAYRCIRVNKFQRVDP
  DVLKACENSCILYSDLGLPKELTLWVDPCEVCCRYGEKNNAFIVASFENKDENKDEISRKVTRALDKVTS
  DYHSGSSSSDEETSKEMEVKPSSVTAAASPVYQISELIFPPLPMWHPLPRKKPGMYRGNGHQNHYPPPVP
  FGYPNQGRKNKPYRPIPVTWVPPPGMHCDRNHWINPHMLAPH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006806
• ORF Size: 756 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006806.5
• RefSeq Size: 1378 bp
• RefSeq ORF: 759 bp
• Locus ID: 10950
• UniProt ID: Q14201
• Cytogenetics: 21q21.1
• Domains: Anti_proliferat
• MW: 28.9 kDa
• Gene Summary: The protein encoded by this gene is a member of the BTG/Tob family. This family has structurally related proteins that appear to have antiproliferative properties. This encoded protein might play a role in neurogenesis in the central nervous system. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]