TTF2 (NM_003594) Human Tagged ORF Clone

CAT#: RC220957

TTF2 (Myc-DDK-tagged)-Human transcription termination factor, RNA polymerase II (TTF2)

Product Images

Circular map for RC220957

• 

  Western blot validation of overexpression lysate (Cat# LY418563) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220957 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TTF2
• Synonyms: F2; HuF2; ZGRF6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220957 representing NM_003594
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAAGTTAGGTGTCCAGAGCACGGGACTTTCTGCTTTCTTAAGACCGGCGTCCGCGATGGCCCGA
  ATAAAGGAAAGAGCTTCTACGTGTGCCGGGCAGACACGTGCAGCTTCGTGCGGGCCACCGACATTCCTGT
  TTCCCATTGCTTATTGCATGAGGACTTTGTGGTAGAGCTTCAGGGTTTGCTTCTGCCACAGGACAAGAAA
  GAATACAGATTGTTCTTCCGATGCATTAGAAGTAAGGCAGAGGGGAAACGCTGGTGTGGAAGTATTCCAT
  GGCAGGATCCTGATTCCAAAGAACATTCTGTATCCAATAAGTCTCAGCATGCATCTGAGACATTTCATCA
  TTCTTCCAACTGGCTGAGAAATCCATTCAAGGTACTTGACAAGAATCAAGAACCAGCTCTCTGGAAACAG
  CTCATCAAAGGTGAAGGTGAGGAAAAGAAGGCTGATAAGAAGCAAAGAGAAAAGGGAGATCAGCTTTTCG
  ATCAAAAGAAAGAACAGAAGCCTGAAATGATGGAGAAAGACCTCTCATCTGGCCTGGTACCAAAGAAAAA
  ACAATCTGTAGTTCAAGAGAAGAAGCAAGAAGAGGGAGCAGAGATTCAGTGTGAGGCAGAGACTGGAGGC
  ACACACAAAAGAGACTTTTCTGAAATTAAATCTCAACAGTGCCAAGGTAATGAGCTTACAAGACCATCTG
  CATCTTCTCAGGAGAAATCAAGTGGTAAGAGTCAAGATGTCCAAAGAGAATCAGAACCTCTGAGAGAAAA
  GGTTACCCAGCTTTTGCCTCAAAATGTTCACAGTCACAACTCAATAAGCAAGCCCCAGAAAGGGGGACCC
  CTCAACAAGGAGTACACGAACTGGGAGGCTAAAGAAACAAAGGCAAAGGATGGCCCTAGCATACAGGCCA
  CCCAGAAAAGCCTGCCTCAGGGGCATTTCCAAGAGCGGCCGGAGACCCACAGTGTGCCTGCTCCTGGAGG
  ACCAGCGGCTCAGGCTGCACCAGCAGCACCAGGGCTTTCCCTGGGTGAGGGCCGTGAAGCTGCCACAAGC
  AGTGACGACGAGGAGGAAGATGATGTTGTTTTTGTTTCCTCTAAGCCTGGGAGCCCCCTACTCTTTGACT
  CGACTCTGGACTTAGAGACGAAGGAAAACCTCCAATTCCCTGATCGAAGTGTGCAAAGAAAGGTGTCTCC
  TGCCTCAGGTGTTTCCAAGAAGGTAGAACCCTCAGACCCAGTAGCCCGGCGTGTCTACCTTACAACACAA
  CTGAAACAAAAGAAGAGCACACTGGCATCAGTGAACATCCAGGCTCTTCCAGACAAGGGTCAGAAGTTGA
  TCAAACAAATCCAGGAGCTGGAGGAAGTACTCAGTGGTCTTACCCTTTCCCCAGAGCAAGGAACTAATGA
  GAAGAGTAACAGTCAAGTACCACAGCAGAGTCACTTCACCAAAACTACCACTGGCCCTCCCCACCTGGTG
  CCTCCCCAACCCCTTCCTCGTCGTGGTACCCAACCTGTGGGTTCTCTAGAACTAAAGTCTGCCTGCCAGG
  TGACTGCTGGAGGATCCAGTCAGTGCTATCGAGGCCATACAAACCAAGATCACGTTCATGCAGTGTGGAA
  AATCACAAGTGAAGCCATCGGTCAACTGCATCGCTCACTTGAGTCATGTCCTGGTGAGACGGTTGTGGCA
  GAAGATCCCGCCGGGCTGAAGGTCCCTTTGCTACTACACCAGAAGCAGGCATTGGCTTGGTTACTATGGC
  GAGAAAGTCAGAAGCCACAAGGAGGAATTCTGGCAGATGATATGGGCTTAGGAAAAACCCTGACAATGAT
  TGCGCTCATCCTGACCCAGAAGAATCAAGAGAAAAAGGAAGAAAAGGAGAAAAGCACAGCTTTGACGTGG
  CTCTCCAAAGATGACTCTTGTGACTTTACTTCCCATGGAACACTAATCATCTGTCCTGCCTCCCTGATCC
  ATCATTGGAAAAATGAGGTGGAGAAACGGGTGAACAGCAACAAACTAAGAGTCTATCTCTACCATGGGCC
  AAACCGGGATTCACGTGCCAGAGTCCTCTCTACATATGACATCGTGATCACTACCTATAGCCTCGTGGCC
  AAGGAGATTCCCACAAACAAGCAAGAGGCAGAGATCCCAGGTGCAAACCTCAATGTGGAGGGCACCTCAA
  CACCTTTGCTTCGAATAGCCTGGGCTCGAATCATATTGGATGAAGCTCACAATGTTAAGAATCCCCGAGT
  GCAGACTTCCATAGCTGTGTGTAAGCTACAAGCCTGTGCCCGTTGGGCTGTCACTGGAACCCCCATTCAA
  AACAACTTATTGGATATGTATTCGCTGCTGAAGTTTCTCCGTTGCTCTCCATTTGATGAGTTCAATCTGT
  GGAGGAGTCAGGTTGACAATGGCTCAAAGAAAGGAGGAGAACGGTTAAGTATTTTAACCAAGAGCCTTTT
  GCTGAGGAGAACAAAAGACCAGCTGGACTCTACTGGCAGACCTTTGGTGATACTGCCCCAGCGTAAATTT
  CAGTTGCACCATTTAAAGCTTTCTGAAGATGAAGAGACTGTTTACAATGTGTTTTTTGCAAGATCAAGGT
  CAGCTCTGCAATCCTATCTAAAAAGACATGAAAGTAGAGGCAACCAATCTGGAAGAAGCCCTAATAATCC
  ATTCAGTAGAGTGGCACTGGAGTTTGGGTCTGAGGAGCCTAGACACTCGGAGGCAGCAGACTCACCGAGA
  TCCAGCACCGTCCACATACTGTCCCAGTTGCTGAGACTCCGCCAGTGTTGCTGTCATCTTTCTTTACTGA
  AGTCGGCCCTGGATCCAATGGAACTGAAGGGTGAAGGTCTTGTCCTTTCCCTGGAAGAACAGCTCAGTGC
  TTTGACCTTGTCAGAACTCCGTGACTCAGAGCCATCTTCCACTGTTTCCCTTAACGGCACCTTCTTCAAG
  ATGGAGCTTTTTGAAGGCATGCGAGAGAGCACCAAGATTTCATCTCTGTTGGCAGAATTGGAGGCAATTC
  AAAGAAATTCAGCATCCCAAAAGAGTGTCATTGTCTCTCAGTGGACCAACATGCTGAAAGTTGTAGCATT
  GCACCTGAAGAAGCATGGACTGACTTATGCCACCATCGATGGCTCTGTCAATCCCAAGCAGAGAATGGAC
  TTGGTAGAGGCATTTAACCACTCCAGAGGCCCTCAGGTAATGCTAATCTCTCTCTTGGCCGGAGGTGTTG
  GTCTAAACCTGACTGGAGGAAATCACCTCTTTCTTTTGGACATGCACTGGAATCCATCACTTGAAGATCA
  AGCTTGTGACCGAATTTACCGAGTAGGGCAGCAGAAAGATGTTGTCATACACAGATTTGTTTGTGAGGGA
  ACAGTAGAAGAAAAGATCTTACAGCTCCAAGAAAAAAAGAAAGATTTGGCCAAACAAGTTCTATCAGGGT
  CTGGAGAATCTGTCACCAAGCTCACCTTGGCTGACCTCAGAGTCCTTTTTGGCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220957 representing NM_003594
  Red=Cloning site Green=Tags(s)
  
  MEEVRCPEHGTFCFLKTGVRDGPNKGKSFYVCRADTCSFVRATDIPVSHCLLHEDFVVELQGLLLPQDKK
  EYRLFFRCIRSKAEGKRWCGSIPWQDPDSKEHSVSNKSQHASETFHHSSNWLRNPFKVLDKNQEPALWKQ
  LIKGEGEEKKADKKQREKGDQLFDQKKEQKPEMMEKDLSSGLVPKKKQSVVQEKKQEEGAEIQCEAETGG
  THKRDFSEIKSQQCQGNELTRPSASSQEKSSGKSQDVQRESEPLREKVTQLLPQNVHSHNSISKPQKGGP
  LNKEYTNWEAKETKAKDGPSIQATQKSLPQGHFQERPETHSVPAPGGPAAQAAPAAPGLSLGEGREAATS
  SDDEEEDDVVFVSSKPGSPLLFDSTLDLETKENLQFPDRSVQRKVSPASGVSKKVEPSDPVARRVYLTTQ
  LKQKKSTLASVNIQALPDKGQKLIKQIQELEEVLSGLTLSPEQGTNEKSNSQVPQQSHFTKTTTGPPHLV
  PPQPLPRRGTQPVGSLELKSACQVTAGGSSQCYRGHTNQDHVHAVWKITSEAIGQLHRSLESCPGETVVA
  EDPAGLKVPLLLHQKQALAWLLWRESQKPQGGILADDMGLGKTLTMIALILTQKNQEKKEEKEKSTALTW
  LSKDDSCDFTSHGTLIICPASLIHHWKNEVEKRVNSNKLRVYLYHGPNRDSRARVLSTYDIVITTYSLVA
  KEIPTNKQEAEIPGANLNVEGTSTPLLRIAWARIILDEAHNVKNPRVQTSIAVCKLQACARWAVTGTPIQ
  NNLLDMYSLLKFLRCSPFDEFNLWRSQVDNGSKKGGERLSILTKSLLLRRTKDQLDSTGRPLVILPQRKF
  QLHHLKLSEDEETVYNVFFARSRSALQSYLKRHESRGNQSGRSPNNPFSRVALEFGSEEPRHSEAADSPR
  SSTVHILSQLLRLRQCCCHLSLLKSALDPMELKGEGLVLSLEEQLSALTLSELRDSEPSSTVSLNGTFFK
  MELFEGMRESTKISSLLAELEAIQRNSASQKSVIVSQWTNMLKVVALHLKKHGLTYATIDGSVNPKQRMD
  LVEAFNHSRGPQVMLISLLAGGVGLNLTGGNHLFLLDMHWNPSLEDQACDRIYRVGQQKDVVIHRFVCEG
  TVEEKILQLQEKKKDLAKQVLSGSGESVTKLTLADLRVLFGI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003594
• ORF Size: 3486 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003594.4
• RefSeq Size: 4860 bp
• RefSeq ORF: 3489 bp
• Locus ID: 8458
• UniProt ID: Q9UNY4
• Cytogenetics: 1p13.1
• Domains: SNF2_N, DEAD, helicase_C
• Protein Families: Transcription Factors
• MW: 129.4 kDa
• Gene Summary: This gene encodes a member of the SWI2/SNF2 family of proteins, which play a critical role in altering protein-DNA interactions. The encoded protein has been shown to have dsDNA-dependent ATPase activity and RNA polymerase II termination activity. This protein interacts with cell division cycle 5-like, associates with human splicing complexes, and plays a role in pre-mRNA splicing. [provided by RefSeq, Jul 2008]