TCF20 (NM_005650) Human Tagged ORF Clone

CAT#: RC220830

TCF20 (Myc-DDK-tagged)-Human transcription factor 20 (AR1) (TCF20), transcript variant 1

Product Images

Circular map for RC220830

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TCF20
• Synonyms: AR1; DDVIBA; SPBP; TCF-20
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220830 representing NM_005650
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGTCCTTTCGGGAGCAAAGCAGTTACCACGGAAACCAGCAAAGCTACCCACAGGAGGTACACGGCT
  CATCCCGGCTAGAAGAGTTCAGCCCTCGTCAGGCCCAGATGTTCCAGAATTTTGGAGGTACAGGTGGCAG
  TAGTGGCAGCAGTGGCAGTGGCAGTGGTGGTGGACGACGAGGAGCAGCAGCTGCTGCGGCAGCGATGGCT
  AGCGAGACCTCTGGCCATCAAGGTTACCAGGGTTTCAGGAAAGAGGCTGGAGATTTTTACTACATGGCAG
  GCAACAAAGACCCCGTGACTACAGGAACCCCACAGCCTCCTCAGCGAAGGCCTTCTGGGCCTGTGCAGAG
  CTATGGACCCCCCCAGGGGAGCAGCTTTGGCAATCAGTATGGGAGTGAGGGTCATGTGGGCCAGTTTCAA
  GCACAGCACTCTGGCCTTGGCGGTGTGTCACATTATCAGCAGGATTACACTGGGCCTTTCTCTCCAGGGA
  GTGCTCAGTACCAACAGCAGGCTTCCAGCCAGCAGCAGCAGCAGCAAGTCCAGCAGTTGAGACAACAGCT
  TTACCAGTCCCATCAGCCCCTGCCACAGGCCACTGGCCAACCAGCATCCAGCTCATCCCATCTACAGCCA
  ATGCAGCGGCCCTCAACTCTGCCATCCTCTGCTGCTGGTTACCAGTTAAGAGTGGGTCAGTTTGGCCAAC
  ACTATCAGTCTTCTGCTTCCTCCTCCTCCTCCTCCTCCTTCCCTTCACCACAGCGTTTTAGCCAGTCTGG
  ACAGAGCTATGATGGCAGTTACAATGTGAATGCTGGATCTCAGTATGAAGGACACAATGTGGGTTCTAAT
  GCACAGGCTTATGGAACACAATCCAATTACAGCTATCAGCCTCAATCTATGAAGAATTTTGAACAGGCAA
  AGATTCCACAAGGGACCCAACAGGGGCAGCAGCAGCAGCAACCGCAGCAACAACAACACCCTTCTCAGCA
  TGTGATGCAGTATACTAACGCTGCCACCAAGCTGCCCCTGCAAAGCCAAGTGGGGCAGTACAACCAGCCT
  GAGGTTCCTGTGAGGTCCCCCATGCAGTTTCACCAGAACTTCAGCCCCATTTCTAACCCTTCTCCAGCTG
  CCTCTGTGGTTCAGTCTCCAAGCTGTAGTTCTACCCCATCTCCTCTCATGCAGACTGGGGAGAATCTCCA
  GTGTGGGCAAGGCAGTGTGCCTATGGGTTCCAGAAACAGAATTTTACAGTTAATGCCTCAACTCAGTCCA
  ACCCCATCAATGATGCCCAGTCCTAATTCTCATGCTGCAGGCTTCAAAGGGTTTGGACTAGAAGGGGTAC
  CAGAAAAGCGACTGACAGATCCTGGGTTGAGTAGTTTGAGTGCTCTGAGTACTCAAGTGGCCAATCTTCC
  TAACACTGTCCAGCACATGTTACTTTCTGATGCCCTGACTCCTCAGAAGAAGACCTCCAAGAGGCCCTCA
  TCTTCCAAGAAAGCAGATAGCTGCACAAATTCTGAAGGCTCCTCACAACCTGAAGAACAGCTGAAGTCCC
  CTATGGCAGAGTCATTAGATGGAGGCTGCTCCAGCAGTTCAGAGGATCAAGGCGAGAGAGTGCGGCAACT
  AAGTGGCCAGAGCACCAGCTCTGACACCACCTACAAGGGTGGAGCCTCTGAGAAAGCTGGCTCCTCACCG
  GCACAAGGTGCTCAGAATGAACCCCCCAGACTCAATGCTAGTCCTGCCGCAAGAGAAGAGGCCACCTCAC
  CAGGCGCTAAGGACATGCCATTGTCATCCGACGGGAACCCAAAGGTTAATGAGAAGACTGTTGGGGTGAT
  TGTCTCCCGGGAAGCCATGACAGGTCGGGTAGAAAAGCCTGGTGGACAAGATAAAGGCTCCCAAGAGGAT
  GATCCTGCAGCCACTCAAAGGCCACCTAGCAATGGTGGGGCAAAGGAAACCAGTCATGCATCACTTCCCC
  AGCCAGAGCCTCCAGGAGGAGGAGGGAGCAAAGGAAACAAGAATGGCGATAACAACTCCAACCATAATGG
  AGAAGGAAATGGCCAGAGTGGCCACTCTGCAGCGGGCCCTGGTTTTACGAGCAGAACTGAGCCTAGCAAA
  TCTCCTGGAAGTCTGCGCTATAGTTACAAAGATAGTTTCGGGTCAGCCGTGCCACGAAATGTCAGTGGCT
  TTCCTCAGTATCCTACAGGGCAAGAAAAGGGAGATTTCACTGGCCATGGGGAACGAAAGGGTAGAAATGA
  AAAATTCCCAAGCCTCCTGCAGGAAGTGCTTCAGGGTTACCACCACCACCCTGACAGGAGATATTCTAGG
  AGTACTCAAGAGCATCAGGGGATGGCTGGTAGCCTAGAAGGAACCACAAGGCCCAATGTCTTGGTTAGTC
  AAACCAATGAATTAGCTAGCAGGGGCCTTCTGAACAAAAGCATTGGGTCTCTATTAGAAAATCCCCACTG
  GGGCCCCTGGGAAAGGAAATCAAGCAGCACAGCTCCTGAAATGAAACAGATCAATTTGACTGACTATCCA
  ATTCCCAGAAAGTTTGAAATAGAGCCTCAGTCATCAGCACATGAGCCTGGGGGTTCCCTCTCTGAAAGAA
  GATCAGTGATCTGTGATATTTCTCCACTAAGACAGATTGTCAGGGACCCAGGGGCTCACTCACTGGGACA
  CATGAGTGCCGACACCAGAATTGGGAGGAATGACCGTCTCAATCCAACTTTAAGTCAGTCGGTCATTCTT
  CCTGGTGGTTTGGTGTCCATGGAAACCAAGCTGAAATCCCAGAGCGGGCAGATAAAAGAGGAAGACTTTG
  AACAGTCTAAATCTCAAGCTAGTTTCAACAACAAGAAATCTGGAGACCACTGCCATCCTCCTAGCATCAA
  GCATGAGTCTTACCGCGGCAATGCCAGCCCTGGAGCAGCAACCCATGATTCCCTTTCAGACTATGGCCCG
  CAAGACAGCAGACCCACGCCAATGCGGCGGGTCCCTGGCAGAGTTGGTGGTCGGGAGGGCATGAGGGGTC
  GGTCCCCTTCTCAATATCATGACTTTGCAGAAAAATTGAAAATGTCTCCTGGGCGGAGCAGAGGCCCAGG
  GGGAGACCCTCATCACATGAATCCACACATGACCTTTTCAGAGAGGGCTAACCGGAGTTCTTTACACACT
  CCCTTTTCTCCCAACTCAGAAACCCTGGCCTCTGCTTATCATGCAAATACTCGGGCTCATGCTTATGGGG
  ACCCTAACGCAGGTTTGAATTCTCAGCTGCATTATAAGAGACAGATGTACCAACAGCAACCAGAGGAGTA
  TAAAGACTGGAGCAGCGGTTCTGCTCAGGGAGTAATTGCTGCAGCACAGCACAGGCAGGAGGGGCCACGG
  AAGAGTCCAAGGCAGCAGCAGTTTCTTGACAGAGTACGGAGCCCTCTGAAAAATGACAAAGATGGTATGA
  TGTATGGCCCACCAGTGGGGACTTACCATGACCCCAGTGCCCAGGAGGCTGGGCGCTGCCTAATGTCTAG
  TGATGGTCTGCCTAACAAGGGCATGGAATTAAAGCATGGCTCCCAGAAGTTACAAGAATCCTGTTGGGAT
  CTTTCTCGGCAAACTTCTCCAGCCAAAAGCAGCGGTCCTCCAGGAATGTCCAGTCAAAAAAGGTATGGGC
  CGCCCCATGAGACTGATGGACATGGACTAGCTGAGGCTACACAGTCATCCAAACCTGGTAGTGTTATGCT
  GAGACTTCCAGGCCAGGAGGATCATTCTTCTCAAAACCCCTTAATCATGAGGAGGCGTGTTCGTTCTTTT
  ATCTCTCCCATTCCCAGTAAGAGACAGTCACAAGATGTAAAGAACAGTAGCACTGAAGATAAAGGTCGCC
  TCCTTCACTCATCAAAAGAAGGCGCTGATAAAGCATTCAATTCCTATGCCCATCTTTCTCACAGTCAGGA
  TATCAAGTCTATCCCTAAGAGAGATTCCTCCAAGGACCTTCCAAGTCCAGATAGTAGAAACTGCCCTGCT
  GTTACCCTCACAAGCCCTGCTAAGACCAAAATACTGCCCCCACGGAAAGGACGGGGATTGAAATTGGAAG
  CTATAGTTCAGAAGATTACATCCCCAAATATTAGGAGGAGCGCATCTTCGAACAGTGCGGAGGCTGGGGG
  AGACACGGTTACGCTTGATGATATACTGTCTTTGAAGAGTGGTCCTCCTGAAGGTGGGAGTGTTGCTGTT
  CAGGATGCTGACATAGAGAAGAGAAAAGGTGAGGTGGCTTCGGACCTAGTCAGTCCAGCAAACCAGGAGT
  TGCACGTAGAGAAACCTCTTCCAAGGTCTTCAGAAGAGTGGCGTGGCAGCGTGGATGACAAAGTGAAGAC
  AGAGACACATGCAGAAACAGTTACTGCCGGAAAGGAACCCCCTGGTGCCATGACATCCACAACCTCACAG
  AAGCCTGGTAGTAACCAAGGGAGACCAGATGGTTCCCTGGGTGGAACAGCACCTTTAATCTTTCCAGACT
  CAAAGAATGTACCTCCAGTGGGCATATTGGCCCCTGAGGCAAACCCCAAGGCTGAAGAGAAGGAGAACGA
  TACAGTGACGATTTCACCGAAGCAAGAGGGTTTCCCTCCAAAGGGATATTTCCCATCAGGAAAGAAGAAG
  GGGAGACCCATTGGTAGTGTGAATAAGCAAAAGAAACAGCAGCAGCCACCGCCTCCACCCCCTCAGCCCC
  CACAGATACCAGAAGGTTCTGCAGATGGAGAGCCAAAGCCAAAAAAACAGAGGCAAAGGAGGGAGAGAAG
  GAAGCCTGGGGCCCAGCCGAGGAAGCGAAAAACCAAACAAGCAGTTCCCATTGTGGAACCCCAAGAACCT
  GAGATCAAACTAAAATATGCCACCCAGCCACTGGATAAAACTGATGCCAAGAACAAGTCTTTTTACCCTT
  ACATCCATGTAGTAAATAAGTGTGAACTTGGAGCCGTTTGTACAATCATCAATGCTGAGGAAGAAGAACA
  GACCAAATTAGTGAGGGGCAGGAAGGGTCAGAGGTCACTGACCCCTCCACCTAGCAGCACTGAAAGCAAG
  GCGCTCCCGGCCTCGTCCTTTATGCTGCAGGGACCTGTTGTGACAGAGTCTTCGGTTATGGGGCACCTGG
  TTTGCTGTCTGTGTGGCAAGTGGGCCAGTTACCGGAACATGGGTGACCTCTTTGGACCTTTTTATCCCCA
  AGATTATGCAGCCACTCTCCCGAAGAATCCACCTCCTAAGAGGGCCACAGAAATGCAGAGCAAAGTTAAG
  GTACGGCACAAAAGTGCTTCTAATGGCTCCAAGACGGACACTGAGGAGGAGGAAGAGCAGCAGCAGCAGC
  AGAAGGAGCAGAGAAGCCTGGCCGCACACCCCAGGTTTAAGCGGCGCCACCGCTCGGAAGACTGTGGTGG
  AGGCCCTCGGTCCCTGTCCAGGGGGCTCCCTTGTAAAAAAGCAGCCACTGAGGGCAGCAGTGAAAAGACT
  GTTTTGGACTCGAAGCCCTCCGTGCCCACCACTTCAGAAGGTGGCCCTGAGCTGGAGTTACAAATCCCTG
  AACTACCTCTTGACAGCAATGAATTTTGGGTCCATGAGGGTTGTATTCTCTGGGCCAATGGAATCTACCT
  GGTTTGTGGCAGGCTCTATGGCCTGCAGGAAGCGCTGGAAATAGCCAGAGAGATGAAATGTTCCCACTGC
  CAGGAGGCAGGCGCCACCTTGGGCTGCTACAACAAAGGCTGCTCCTTCCGATACCATTACCCGTGTGCCA
  TTGATGCAGATTGTTTGCTACATGAGGAGAACTTCTCGGTGAGGTGCCCTAAGCACAAGCCTCCCCTTCC
  GTGCCCTCTCCCCCCCTTGCAGAACAAGACCGCGAAAGGCAGCCTCAGCACAGAGCAGTCGGAGCGGGGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220830 representing NM_005650
  Red=Cloning site Green=Tags(s)
  
  MQSFREQSSYHGNQQSYPQEVHGSSRLEEFSPRQAQMFQNFGGTGGSSGSSGSGSGGGRRGAAAAAAAMA
  SETSGHQGYQGFRKEAGDFYYMAGNKDPVTTGTPQPPQRRPSGPVQSYGPPQGSSFGNQYGSEGHVGQFQ
  AQHSGLGGVSHYQQDYTGPFSPGSAQYQQQASSQQQQQQVQQLRQQLYQSHQPLPQATGQPASSSSHLQP
  MQRPSTLPSSAAGYQLRVGQFGQHYQSSASSSSSSSFPSPQRFSQSGQSYDGSYNVNAGSQYEGHNVGSN
  AQAYGTQSNYSYQPQSMKNFEQAKIPQGTQQGQQQQQPQQQQHPSQHVMQYTNAATKLPLQSQVGQYNQP
  EVPVRSPMQFHQNFSPISNPSPAASVVQSPSCSSTPSPLMQTGENLQCGQGSVPMGSRNRILQLMPQLSP
  TPSMMPSPNSHAAGFKGFGLEGVPEKRLTDPGLSSLSALSTQVANLPNTVQHMLLSDALTPQKKTSKRPS
  SSKKADSCTNSEGSSQPEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSP
  AQGAQNEPPRLNASPAAREEATSPGAKDMPLSSDGNPKVNEKTVGVIVSREAMTGRVEKPGGQDKGSQED
  DPAATQRPPSNGGAKETSHASLPQPEPPGGGGSKGNKNGDNNSNHNGEGNGQSGHSAAGPGFTSRTEPSK
  SPGSLRYSYKDSFGSAVPRNVSGFPQYPTGQEKGDFTGHGERKGRNEKFPSLLQEVLQGYHHHPDRRYSR
  STQEHQGMAGSLEGTTRPNVLVSQTNELASRGLLNKSIGSLLENPHWGPWERKSSSTAPEMKQINLTDYP
  IPRKFEIEPQSSAHEPGGSLSERRSVICDISPLRQIVRDPGAHSLGHMSADTRIGRNDRLNPTLSQSVIL
  PGGLVSMETKLKSQSGQIKEEDFEQSKSQASFNNKKSGDHCHPPSIKHESYRGNASPGAATHDSLSDYGP
  QDSRPTPMRRVPGRVGGREGMRGRSPSQYHDFAEKLKMSPGRSRGPGGDPHHMNPHMTFSERANRSSLHT
  PFSPNSETLASAYHANTRAHAYGDPNAGLNSQLHYKRQMYQQQPEEYKDWSSGSAQGVIAAAQHRQEGPR
  KSPRQQQFLDRVRSPLKNDKDGMMYGPPVGTYHDPSAQEAGRCLMSSDGLPNKGMELKHGSQKLQESCWD
  LSRQTSPAKSSGPPGMSSQKRYGPPHETDGHGLAEATQSSKPGSVMLRLPGQEDHSSQNPLIMRRRVRSF
  ISPIPSKRQSQDVKNSSTEDKGRLLHSSKEGADKAFNSYAHLSHSQDIKSIPKRDSSKDLPSPDSRNCPA
  VTLTSPAKTKILPPRKGRGLKLEAIVQKITSPNIRRSASSNSAEAGGDTVTLDDILSLKSGPPEGGSVAV
  QDADIEKRKGEVASDLVSPANQELHVEKPLPRSSEEWRGSVDDKVKTETHAETVTAGKEPPGAMTSTTSQ
  KPGSNQGRPDGSLGGTAPLIFPDSKNVPPVGILAPEANPKAEEKENDTVTISPKQEGFPPKGYFPSGKKK
  GRPIGSVNKQKKQQQPPPPPPQPPQIPEGSADGEPKPKKQRQRRERRKPGAQPRKRKTKQAVPIVEPQEP
  EIKLKYATQPLDKTDAKNKSFYPYIHVVNKCELGAVCTIINAEEEEQTKLVRGRKGQRSLTPPPSSTESK
  ALPASSFMLQGPVVTESSVMGHLVCCLCGKWASYRNMGDLFGPFYPQDYAATLPKNPPPKRATEMQSKVK
  VRHKSASNGSKTDTEEEEEQQQQQKEQRSLAAHPRFKRRHRSEDCGGGPRSLSRGLPCKKAATEGSSEKT
  VLDSKPSVPTTSEGGPELELQIPELPLDSNEFWVHEGCILWANGIYLVCGRLYGLQEALEIAREMKCSHC
  QEAGATLGCYNKGCSFRYHYPCAIDADCLLHEENFSVRCPKHKPPLPCPLPPLQNKTAKGSLSTEQSERG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005650
• ORF Size: 5880 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005650.4
• RefSeq Size: 7407 bp
• RefSeq ORF: 5883 bp
• Locus ID: 6942
• UniProt ID: Q9UGU0
• Cytogenetics: 22q13.3
• Protein Families: Druggable Genome, Transcription Factors
• MW: 211.6 kDa
• Gene Summary: This gene encodes a transcription factor that recognizes the platelet-derived growth factor-responsive element in the matrix metalloproteinase 3 promoter. The encoded protein is thought to be a transcriptional coactivator, enhancing the activity of transcription factors such as JUN and SP1. Mutations in this gene are associated with autism spectrum disorders. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]