ABCG8 (NM_022437) Human Tagged ORF Clone

CAT#: RC220827

ABCG8 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family G (WHITE), member 8 (ABCG8)

Product Images

Circular map for RC220827

• 

  Western blot validation of overexpression lysate (Cat# LY411701) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220827 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ABCG8
• Synonyms: GBD4; STSL; STSL1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220827 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGGAAGGCGGCAGAGGAGAGAGGGCTGCCGAAAGGGGCCACTCCCCAGCATACCTCGGGCCTCC
  AGGATAGATTGTTCTCCTCTGAAAGTGACAACAGCCTGTACTTCACCTACAGTGGCCAGCCCAACACCCT
  GGAGGTCAGAGACCTCAACTACCAGGTGGACCTGGCCTCTCAGGTCCCTTGGTTTGAGCAGCTGGCTCAG
  TTCAAGATGCCCTGGACATCTCCCAGCTGCCAGAATTCTTGTGAGCTGGGCATCCAGAACCTAAGCTTCA
  AAGTGAGAAGTGGGCAGATGCTGGCCATCATAGGGAGCTCAGGTTGTGGGAGAGCCTCCTTGCTAGATGT
  GATCACTGGCCGAGGTCACGGCGGCAAGATCAAGTCAGGCCAGATCTGGATCAATGGGCAGCCCAGCTCG
  CCTCAGCTGGTGAGGAAGTGTGTGGCCCACGTGCGCCAGCACAACCAGCTGCTCCCCAACTTGACTGTGC
  GAGAGACCTTGGCCTTCATTGCCCAGATGCGGCTGCCCAGAACCTTCTCCCAGGCCCAGCGTGACAAAAG
  GGTGGAGGACGTGATCGCGGAGCTGCGGCTTAGGCAGTGCGCTGACACCCGCGTGGGCAACATGTACGTG
  CGGGGGTTGTCGGGGGGTGAGCGCAGGAGAGTCAGCATTGGGGTGCAGCTCCTGTGGAACCCAGGAATCC
  TTATTCTCGACGAACCCACCTCTGGGCTCGACAGCTTCACAGCCCACAACCTGGTGAAGACCTTGTCCAG
  GCTGGCCAAAGGCAACCGGCTGGTGCTCATCTCCCTCCACCAGCCTCGCTCTGACATCTTCAGGCTGTTT
  GATCTGGTCCTCCTGATGACGTCTGGCACCCCCATCTACTTAGGGGCGGCCCAGCACATGGTCCAGTATT
  TCACAGCCATCGGCTACCCCTGTCCTCGCTACAGCAATCCTGCTGACTTCTATGTGGACCTGACCAGCAT
  TGACAGGCGCAGCAGAGAGCAGGAATTGGCCACCAGGGAGAAGGCTCAGTCACTCGCAGCCCTGTTTCTA
  GAAAAAGTGCGTGACTTAGATGACTTTCTATGGAAAGCAGAGACGAAGGATCTTGACGAGGACACCTGTG
  TGGAAAGCAGCGTGACCCCACTAGACACCAACTGCCTCCCGAGTCCTACGAAGATGCCTGGGGCGGTGCA
  GCAGTTTACGACGCTGATCCGTCGTCAGATTTCCAACGACTTCCGAGACCTGCCCACCCTCCTCATCCAT
  GGGGCGGAGGCCTGTCTGATGTCAATGACCATCGGCTTCCTCTATTTTGGCCATGGGAGCATCCAGCTCT
  CCTTCATGGATACAGCCGCCCTCTTGTTCATGATCGGTGCTCTCATCCCTTTCAACGTCATTCTGGATGT
  CATCTCCAAATGTTACTCAGAGAGGGCAATGCTTTACTATGAACTGGAAGACGGGCTGTACACCACTGGT
  CCATATTTCTTTGCCAAGATCCTCGGGGAGCTTCCGGAGCACTGTGCCTACATCATCATCTACGGGATGC
  CCACCTACTGGCTGGCCAACCTGAGGCCAGGCCTCCAGCCCTTCCTGCTGCACTTCCTGCTGGTGTGGCT
  GGTGGTCTTCTGTTGCAGGATTATGGCCCTGGCCGCCGCGGCCCTGCTCCCCACCTTCCACATGGCCTCC
  TTCTTCAGCAATGCCCTCTACAACTCCTTCTACCTCGCCGGGGGCTTCATGATAAACTTGAGCAGCCTGT
  GGACAGTGCCCGCGTGGATTTCCAAAGTGTCCTTCCTGCGGTGGTGTTTTGAAGGGCTGATGAAGATTCA
  GTTCAGCAGAAGAACTTATAAAATGCCTCTCGGGAACCTCACCATCGCGGTCTCAGGAGATAAAATCCTC
  AGTGCCATGGAGCTGGACTCGTACCCTCTCTACGCCATCTACCTCATCGTCATTGGCCTCAGCGGTGGCT
  TCATGGTCCTGTACTACGTGTCCTTAAGGTTCATCAAACAGAAACCAAGTCAAGACTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220827 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGKAAEERGLPKGATPQHTSGLQDRLFSSESDNSLYFTYSGQPNTLEVRDLNYQVDLASQVPWFEQLAQ
  FKMPWTSPSCQNSCELGIQNLSFKVRSGQMLAIIGSSGCGRASLLDVITGRGHGGKIKSGQIWINGQPSS
  PQLVRKCVAHVRQHNQLLPNLTVRETLAFIAQMRLPRTFSQAQRDKRVEDVIAELRLRQCADTRVGNMYV
  RGLSGGERRRVSIGVQLLWNPGILILDEPTSGLDSFTAHNLVKTLSRLAKGNRLVLISLHQPRSDIFRLF
  DLVLLMTSGTPIYLGAAQHMVQYFTAIGYPCPRYSNPADFYVDLTSIDRRSREQELATREKAQSLAALFL
  EKVRDLDDFLWKAETKDLDEDTCVESSVTPLDTNCLPSPTKMPGAVQQFTTLIRRQISNDFRDLPTLLIH
  GAEACLMSMTIGFLYFGHGSIQLSFMDTAALLFMIGALIPFNVILDVISKCYSERAMLYYELEDGLYTTG
  PYFFAKILGELPEHCAYIIIYGMPTYWLANLRPGLQPFLLHFLLVWLVVFCCRIMALAAAALLPTFHMAS
  FFSNALYNSFYLAGGFMINLSSLWTVPAWISKVSFLRWCFEGLMKIQFSRRTYKMPLGNLTIAVSGDKIL
  SAMELDSYPLYAIYLIVIGLSGGFMVLYYVSLRFIKQKPSQDW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022437
• ORF Size: 2019 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022437.3
• RefSeq Size: 2679 bp
• RefSeq ORF: 2022 bp
• Locus ID: 64241
• UniProt ID: Q9H221
• Cytogenetics: 2p21
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ABC transporters
• MW: 75.7 kDa
• Gene Summary: The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. The protein encoded by this gene functions to exclude non-cholesterol sterol entry at the intestinal level, promote excretion of cholesterol and sterols into bile, and to facilitate transport of sterols back into the intestinal lumen. It is expressed in a tissue-specific manner in the liver, intestine, and gallbladder. This gene is tandemly arrayed on chromosome 2, in a head-to-head orientation with family member ABCG5. Mutations in this gene may contribute to sterol accumulation and atherosclerosis, and have been observed in patients with sitosterolemia. [provided by RefSeq, Jul 2008]