GAS2L2 (NM_139285) Human Tagged ORF Clone

CAT#: RC220666

GAS2L2 (Myc-DDK-tagged)-Human growth arrest-specific 2 like 2 (GAS2L2)

Product Images

Circular map for RC220666

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GAS2L2
• Synonyms: CILD41; GAR17
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220666 representing NM_139285
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCAGCCTGCGGGAGGCAGGAGGAAGCCCAGGACCCTAGGGCCGCCTGTGTGCAGTATCCGGCCTT
  TCAAGTCGAGTGAGCAGTACCTGGAGGCCATGAAGGAAGACCTGGCTGAGTGGCTTCGCGACCTCTATGG
  GCTGGACATCGACGCAGCCAACTTCCTGCAGGTGCTGGAAACGGGCCTGGTGCTGTGCCAACACGCCAAC
  GTTGTCACTGACGCTGCCCTGGCCTTCCTGGCTGAGGCACCTGCCCAAGCCCAGAAGATTCCCATGCCCC
  GGGTCGGGGTCTCCTGCAATGGGGCCGCCCAGCCAGGTACCTTCCAGGCCAGGGACAATGTCTCTAACTT
  CATCCAGTGGTGTCGAAAGGAGATGGGCATCCAAGAGGTGCTGATGTTCGAGACGGAGGACTTGGTGCTG
  CGCAAGAACGTGAAGAACGTGGTGCTGTGTTTGCTGGAGCTGGGCCGCCGGGCGTGGCGCTTTGGTGTTG
  CGGCGCCCACACTCGTGCAGCTGGAGGAGGAGATCGAGGAGGAGGTGCGGCGGGAGCTGGCCCTGCCCCC
  GCCCGACCCCTCGCCGCCAGCGCCCCCCAGGCGCCAGCCCTGCCACTTCCGCAACCTGGACCAGATGGTG
  CAGAGCCTTGTGAGCCACTGCACGTGCCCAGTGCAGTTCTCCATGGTCAAAGTGTCTGAGGGGAAGTACC
  GTGTGGGTGACTCCAACACCCTCATCTTCATCCGGATCCTCCGGAACCATGTGATGGTACGTGTAGGGGG
  CGGCTGGGACACACTGGGCCATTACCTGGACAAACATGACCCCTGCCGCTGCACATCCCTCTCACACAAG
  CCAGGCAGCTTCCTGAAGCCCCCGGCCCCACCAGTGCAGCATGAAGTAAGGGTACAGGATGGACCCTCAC
  AGACCCAGCCTACAATGACCATCAGCCGCTCACAGAGCCCACCACCCCCTGTGGACTGGAAGACATATAC
  CTCTTCAGACCGAAGGCTGAGGCCCCCCACCCCATCCTCCCCCAGACCCCGCAGGGAACGGGGAGCAGGG
  ACGGGGGCCTCCAGAGAGATGGCACCATTCCTGAGGTGCCAGGAGAGGTCTCTCATCCCATCTTGGAGGC
  AGCCGACAGCTGGGGACAGCCCACCCAGCCCCCAGTCCTCATCTACCCAAAAAGGCCGAGACCCACAGTG
  TACCTCGTCAGGAAAGAGGGAGGAGAGATACCCCCCTGAACTCCCCAGGGGAAGGATTCCCACATCTTGG
  GTTCATGAAGAAACAGACAGCTGGGGAACCGACGCCGGGAACCCCACCCCACAAAGACTCCGAGCCATTG
  AGGCCACCACCAAAGGGATATCAGCAAGAGGACCATCTCCCCTGCCTCGTTCCTTTGGCCCAGCCGAGTG
  CCTGGGCCTCAGGCTGCCACTCCGGGATGAGGCCAAGGGTGCGTTCTTCCAGTTCAGGGAGCCAGAGTCT
  GTCCGTTCTCCAACCCCTGTCCAAGGCCTAACCAAGATCCCCATCCGGCTGCCCCCTGCTCGCCCCCCAA
  CACCAGGAAGGAGCTTTCCTGGTGCTACAAGTGGAAGTCCCAGGACAGAACTTGGGAGAGACCCCATCCC
  ACTAAGGGCCGTCACTGTGGACCTGGCTGGGTCCACGCATGGGGACTGCTCTGTGGAAGTGAGGCAGGAG
  GACCAGCAGCTGGACATCCAGGTCATGGCAGAGGCCAGAGAGTCCTGGGACCTGGGCCTACAGGAGCAGG
  AGGGGCGGTACACACCTCTGCCCTTGGGCGGGAACAAGGAGCAAGCCATCTACTGTAGCCTTGAAGAGGA
  GATTTTGGGCAACATGAAGCTGCTAGAAGTCAGGAGTGCCTGTCCGCAGGGCACAAGGTCTGGGGTCATC
  CCTCGCAGTGGGGTCTACATCCCCAGGCTGGCTGGGCAGTGGCCTGAGCCTGGGGGTCCTTATGACAAAG
  CCATCCAAGAACTGGCTCAGGGGTCCCCATCCCTCCTTAAAGTGGACCTGGAAGCCTGGAAGGCAGCCCC
  GACTGGCTCCCCTAAGCCAGCTGTTACCCCAGGACCGGGAAGCCTCAAAGGGAAGTTGGGAGCCAGACAG
  AGTGGGCCCAGGACAAAGGCAAGCCTGAGTGCCAAGGGCACCCACATGAGGAAGGTCCCACCTCAGGGAG
  GGCAGGACTGCTCGGCTTCTACTGTGTCTGCCAGCCCGGAGGCCCCCACACCTTCGCCCTTGGACCCCAA
  CTCTGACAAAGCCAAGGCATGTCTGAGCAAGGGCAGGAGAACTCTCCGGAAGCCCAAGAGGGTCCCGTCC
  ATCTACAAGCTGAAGCTGAGACCCAGGATTCGGCCCCGGAGAGACCACAGGCCTGAGAAGCAGCCTTCAC
  GAATCCCCAGGCCACTGGCCTATGTCTTCCTGGGTCCAGCCAGGCAGCCCCCCAAGGACAGGCTGTTGAG
  AGCTGTGCTGGGCAGCAAGGGAGGGGAGGCATCCCGGGTGGATGGAGCTTCAGTAGGTGAGGAGGAGGAG
  GAAGGAAAGGAGGAGAAAGAGCCAGCCGCTCCATTGGAGAGCAGCCCCCAACCTCCAGAGGGCCTGCAAC
  CTCACTGGCTTAATCAAGCTCCACTTCCACCTGAGGAGGAGTCCTGGGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220666 representing NM_139285
  Red=Cloning site Green=Tags(s)
  
  MSQPAGGRRKPRTLGPPVCSIRPFKSSEQYLEAMKEDLAEWLRDLYGLDIDAANFLQVLETGLVLCQHAN
  VVTDAALAFLAEAPAQAQKIPMPRVGVSCNGAAQPGTFQARDNVSNFIQWCRKEMGIQEVLMFETEDLVL
  RKNVKNVVLCLLELGRRAWRFGVAAPTLVQLEEEIEEEVRRELALPPPDPSPPAPPRRQPCHFRNLDQMV
  QSLVSHCTCPVQFSMVKVSEGKYRVGDSNTLIFIRILRNHVMVRVGGGWDTLGHYLDKHDPCRCTSLSHK
  PGSFLKPPAPPVQHEVRVQDGPSQTQPTMTISRSQSPPPPVDWKTYTSSDRRLRPPTPSSPRPRRERGAG
  TGASREMAPFLRCQERSLIPSWRQPTAGDSPPSPQSSSTQKGRDPQCTSSGKREERYPPELPRGRIPTSW
  VHEETDSWGTDAGNPTPQRLRAIEATTKGISARGPSPLPRSFGPAECLGLRLPLRDEAKGAFFQFREPES
  VRSPTPVQGLTKIPIRLPPARPPTPGRSFPGATSGSPRTELGRDPIPLRAVTVDLAGSTHGDCSVEVRQE
  DQQLDIQVMAEARESWDLGLQEQEGRYTPLPLGGNKEQAIYCSLEEEILGNMKLLEVRSACPQGTRSGVI
  PRSGVYIPRLAGQWPEPGGPYDKAIQELAQGSPSLLKVDLEAWKAAPTGSPKPAVTPGPGSLKGKLGARQ
  SGPRTKASLSAKGTHMRKVPPQGGQDCSASTVSASPEAPTPSPLDPNSDKAKACLSKGRRTLRKPKRVPS
  IYKLKLRPRIRPRRDHRPEKQPSRIPRPLAYVFLGPARQPPKDRLLRAVLGSKGGEASRVDGASVGEEEE
  EGKEEKEPAAPLESSPQPPEGLQPHWLNQAPLPPEEESWV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_139285
• ORF Size: 2640 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139285.4
• RefSeq Size: 2712 bp
• RefSeq ORF: 2643 bp
• Locus ID: 246176
• UniProt ID: Q8NHY3
• Cytogenetics: 17q12
• MW: 96.3 kDa
• Gene Summary: The protein encoded by this gene appears to crosslink microtubules and microfilaments and may be part of the cytoskeleton. This gene is mainly expressed in skeletal muscle. [provided by RefSeq, Jul 2011]