NFE4 (NM_001085386) Human Tagged ORF Clone
CAT#: RC220641
NFE4 (Myc-DDK-tagged)-Human transcription factor NF-E4 (NFE4)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_001085386" in other vectors (4)
USD 198.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | NFE4 |
Synonyms | NF-E4 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC220641 representing NM_001085386
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC CTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCCTGCCTCGTGTTGTCTGTTGGCACACTCTCA AGAGTTTGAACGGATACAAGAATCTTTCATCTGGTGCCGAAACCCGGGAGGGGCTCCGGTCTTCGTCCCC CGTGGACCTACCCCTCCGCCCCAGAAAGCAGGCCACAGCAGCCGGACAAAGGAAGCTCCTCAGCCTCCAG TTGCTTCTCTGTGCATGCACATCAGTCACTGATCTCACCTACTGGGGCCCTGCAGGCCATGGGGCCACAG CTCCACACAGAAGCCTCCTAGCAATCCACCTCCACCTGGTGCCTGCTTCAAGTGCGGCAATGAAGGCCAC TGGCCCACACAATGCCCAAACCCAGGTAAACCCACGAGGCCATGCCCCCTCTGCGGAGGACCCCACTGGA AGTTGGACTGTGAGCGGCCCCTGCAAGGACCACCCCCATCCCTTCCTGAGCCAATCAAACCCTCCTACTC GGATCTCGTCAGCCTTGCCGCTGAAGACTGATAGTGCCTTGGAACAGACACCCCAGCAACTACCATCGCT TCATCTGAGCCAAGGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220641 representing NM_001085386
Red=Cloning site Green=Tags(s) LDPVPRRSAAAIALPRVVCWHTLKSLNGYKNLSSGAETREGLRSSSPVDLPLRPRKQATAAGQRKLLSLQ LLLCACTSVTDLTYWGPAGHGATAPHRSLLAIHLHLVPASSAAMKATGPHNAQTQVNPRGHAPSAEDPTG SWTVSGPCKDHPHPFLSQSNPPTRISSALPLKTDSALEQTPQQLPSLHLSQG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001085386 |
ORF Size | 576 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq Size | 967 bp |
RefSeq ORF | 540 bp |
Locus ID | 58160 |
Cytogenetics | 7q22.1 |
MW | 20.33 kDa |
Gene Summary | The erythroid-specific protein encoded by this gene, and the ubiquitous transcription factor CP2, form the stage selector protein (SSP) complex, which is involved in preferential expression of the gamma-globin genes in fetal erythroid cells. Alternate use of an in-frame upstream non-AUG (CUG) translation initiation codon, and a downstream AUG codon, results in two isoforms. While the long isoform (22 kDa) acts as an activator, the short isoform (14 kDa) has been shown to repress gamma-globin gene expression. This gene is located in an intron of the FBXL13 gene on the opposite strand. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC220641L3 | Lenti ORF clone of Human transcription factor NF-E4 (NFE4), Myc-DDK-tagged |
USD 600.00 |
|
RC220641L4 | Lenti ORF clone of Human transcription factor NF-E4 (NFE4), mGFP tagged |
USD 600.00 |
|
RG220641 | NFE4 (tGFP-tagged) - Human transcription factor NF-E4 (NFE4) |
USD 500.00 |
|
SC316149 | NFE4 (untagged)-Human transcription factor NF-E4 (NFE4) |
USD 330.00 |
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