ZNF275 (NM_001080485) Human Tagged ORF Clone

CAT#: RC220608

ZNF275 (Myc-DDK-tagged)-Human zinc finger protein 275 (ZNF275)

Product Images

Circular map for RC220608

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  Western blot validation of overexpression lysate (Cat# LY421040) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220608 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF275
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220608 representing NM_001080485
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGTGGTTGAATCTTTCACATATTTTGCAATTTGGAAGAAGAAGATAATTTATGGAAAGACTGATG
  AGCCCTGGGAATTCTTTCAGGATTTGGTGACATTTGATGATGTGGCATGGTACCTCACCACAAGGGAGTG
  GTTTAAGCTGGACCCTGAACAGAGGGCACTGGAATATTATGGGAACGTGACCTCTGTGGGCGTTCCTGTT
  TTAAATCCTGCCTTGGTCCCTCACCTGGCACAAGGACAAGTGCTACTGGTGTCAGACCCATCGCCCAACA
  CTGATCCTGCTAAGTACTCTGAAAGCACCTCCGCGACCCGACACCAGATGAAGGGGGAAGATGCCCAGCC
  ACAGGAGATGGCGTCCACAAGCTTCCCAAGGGCCAGTGGTCCCAGTCCTGAATTCAGACAGCACGGGGAC
  TCTGACGGGAAGAGAGGGAGCCCACAGAATCTGCCCATAGAACATCATTTTGCTTGTAAAGAGTGTGGGG
  ACACCTTTCGGCTTAAAGTCCTGCTTGTCCAGCACCAGAGAGTCCACAGTGAGGAGAAGGGCTGGGAATG
  TGGCGACTGCGGGAAGGTCTTTAGGGGGGTGGCGGAGTTTAATGAGCACAGGAAAAGCCACGTAGCTGCG
  GAGCCCCAGCCCGGCCCCAGTAGGGCCCTGGAGAATGCCGCGGAGAAGAGGGAGCAGATGGAGAGGGAGG
  CAAAGCCCTTCGAGTGCGAGGAGTGCGGAAAACGGTTTAAGAAGAATGCAGGCCTGAGTCAGCATCTGAG
  GGTCCACAGCAGAGAGAAGCCCTTTGATTGCGAGGAATGCGGGCGGTCGTTCAAAGTCAACACCCACCTC
  TTCCGACATCAGAAACTTCACACTTCGGAAAAGCCTTTCGCCTGCAAGGCGTGCAGCAGGGATTTCCTGG
  ATCGCCAGGAGCTTCTCAAGCACCAGCGCATGCACACTGGCCACCTGCCCTTCGACTGCGACGACTGCGG
  CAAGTCCTTCCGAGGGGTCAACGGGCTGGCCGAGCACCAGCGCATCCACAGTGGGGCCAAGCCATACGGG
  TGTCCCCACTGCGGCAAGCTCTTCCGAAGGAGCTCGGAGCTCACCAAGCACCGGCGGATCCACACGGGCG
  AGAAGCCCTACGCCTGCGGCCAGTGCGGCAAGGCCTTCCGCCAGAGCTCCAGCCTCCTGGAGCACGCACG
  CATCCACAGTGGCGAGCGGCCCTACGCATGCGGCGAGTGTGGCAAGGCCTTCCGTGGGCCCTCTGACCTC
  ATCAAGCACCGGCGCATCCACAGCGGACTGAAACCCTATGAGTGCGACAAATGCGGCAAGGCCTTCCGCC
  GGAGCTCCGGCCTCAGTCGCCACCGGCGGATCCACAGTGGGGCGCGGCGCTGCGAATGCAGCCAGTGTGG
  CCGCGTGTTCAAGAGGCGCTCGGCACTGCAGAAGCATCAGCCAACCCACCACGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220608 representing NM_001080485
  Red=Cloning site Green=Tags(s)
  
  MPVVESFTYFAIWKKKIIYGKTDEPWEFFQDLVTFDDVAWYLTTREWFKLDPEQRALEYYGNVTSVGVPV
  LNPALVPHLAQGQVLLVSDPSPNTDPAKYSESTSATRHQMKGEDAQPQEMASTSFPRASGPSPEFRQHGD
  SDGKRGSPQNLPIEHHFACKECGDTFRLKVLLVQHQRVHSEEKGWECGDCGKVFRGVAEFNEHRKSHVAA
  EPQPGPSRALENAAEKREQMEREAKPFECEECGKRFKKNAGLSQHLRVHSREKPFDCEECGRSFKVNTHL
  FRHQKLHTSEKPFACKACSRDFLDRQELLKHQRMHTGHLPFDCDDCGKSFRGVNGLAEHQRIHSGAKPYG
  CPHCGKLFRRSSELTKHRRIHTGEKPYACGQCGKAFRQSSSLLEHARIHSGERPYACGECGKAFRGPSDL
  IKHRRIHSGLKPYECDKCGKAFRRSSGLSRHRRIHSGARRCECSQCGRVFKRRSALQKHQPTHHE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001080485
• ORF Size: 1455 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080485.1, NP_001073954.1
• RefSeq Size: 5080 bp
• RefSeq ORF: 990 bp
• Locus ID: 10838
• Cytogenetics: Xq28
• MW: 55.38 kDa
• Gene Summary: This gene encodes a zinc finger protein that appears to be conserved in eutheria. Its function has not yet been established. [provided by RefSeq, Jul 2010]