DCAF8L1 (NM_001017930) Human Tagged ORF Clone

CAT#: RC220603

DCAF8L1 (Myc-DDK-tagged)-Human DDB1 and CUL4 associated factor 8-like 1 (DCAF8L1)

Product Images

Circular map for RC220603

• 

  Western blot validation of overexpression lysate (Cat# LY422759) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220603 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DCAF8L1
• Synonyms: WDR42B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220603 representing NM_001017930
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCACCAAGAGGGCAGCACAGGTGGCTTACCAGACTTAGTGACTGAAAGCCTGTTCAGCAGCCCAG
  AGGAGCAGTCTGGAGTAGCAGCGGTGACGGCGGCCTCCTCAGACATTGAAATGGCAGCCACAGAGCCATC
  GACCGGAGATGGTGGTGATACCAGGGATGGTGGTTTCCTGAACGATGCCAGCACAGAAAATCAAAACACA
  GACTCAGAAAGTTCAAGTGAAGACGTCGAACTTGAAAGCATGGGTGAAGGTTTATTTGGTTACCCGTTAG
  TGGGAGAGGAGACAGAAAGGGAGGAGGAAGAAGAAGAGATGGAGGAGGAAGGGGAGGAGGAAGAACAGCC
  TCGGATGTGTCCACGATGCGGTGGCACCAACCATGATCAGTGTTTGTTAGACGAGGATCAGGCGTTGGAG
  GAGTGGATTTCCTCAGAGACATCTGCCCTGCCCCGATCTCGCTGGCAAGTCCTTACTGCTCTTCGCCAGC
  GGCAGCTGGGTTCAAGTGCCCGCTTTGTATATGAGGCCTGTGGGGCAAGAACCTTTGTGCAGCGTTTCCG
  CCTGCAGTATCTTCTTGGAAGCCATGCCGGTTCTGTCAGTACCATACACTTTAACCAGCGTGGCACCCGA
  CTGGCCAGTAGCGGTGATGACTTAAGGGTGATAGTGTGGGACTGGGTGCGGCAGAAGCCAGTACTGAACT
  TTGAGAGTGGTCACGATATTAATGTCATCCAGGCTAAGTTCTTTCCTAACTGTGGTGATTCCACTCTGGC
  CATGTGTGGCCATGATGGACAGGTACGGGTAGCAGAACTAATTAATGCATCATATTGCGAGAATACTAAG
  CGTGTGGCCAAGCACAGGGGACCTGCCCACGAGTTGGCTCTGGAGCCAGACTCTCCTTATAAGTTCCTCA
  CTTCAGGTGAAGATGCCGTTGTGTTCACCATTGACCTCAGGCAAGACCGGCCAGCTTCAAAAGTTGTGGT
  AACAAGAGAAAATGATAAGAAAGTCGGACTGTATACAATCTCTATGAATCCTGCCAATATTTACCAATTT
  GCAGTGGGTGGACATGATCAGTTTGTAAGGATTTATGACCAGAGGAGAATTGATAAGAAAGAAAACAATG
  GAGTACTCAAGAAATTCACTCCTCATCATCTGGTTTATTGTGATTTCCCAACAAACATCACCTGCGTTGT
  GTACAGCCACGATGGCACAGAGCTCCTGGCCAGCTACAATGATGAAGATATTTACCTCTTCAACTCCTCT
  CTCAGTGATGGTGCTCAATATGTTAAGAGATATAAGGGGCACAGAAATAATGACACAATCAAATGTGTTA
  ATTTCTATGGCCCCCGGAGTGAGTTTGTCGTGAGCGGTAGTGATTGTGGGCACGTCTTCTTCTGGGAGAA
  ATCATCCTCCCAGATCATCCAGTTCATGGAGGGGGACAGAGGAGATATAGTAAACTGTCTTGAACCCCAC
  CCTTACCTACCTGTGTTGGCGACCAGTGGCCTAGATCAGCATGTCAGGATCTGGACACCCACAGCTAAAA
  CTGCCACTGAGCTTACTGGGTTAAAAGATGTGATTAAGAAGAACAAGCAGGAGCGAGATGAAGACAACTT
  GAACTATACGGACTCGTTTGACAACCGCATGCTTCGGTTCTTCGTGCGTCACCTGTTACAGAGAGCTCAT
  CAACCCGGCTGGAGAGATCATGGAGCTGAGTTCCCAGATGAAGAAGAGTTGGATGAGTCTTCCAGCACCT
  CAGATACATCCGAGGAGGAGGGCCAAGATCGAGTGCAGTGCATACCATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220603 representing NM_001017930
  Red=Cloning site Green=Tags(s)
  
  MSHQEGSTGGLPDLVTESLFSSPEEQSGVAAVTAASSDIEMAATEPSTGDGGDTRDGGFLNDASTENQNT
  DSESSSEDVELESMGEGLFGYPLVGEETEREEEEEEMEEEGEEEEQPRMCPRCGGTNHDQCLLDEDQALE
  EWISSETSALPRSRWQVLTALRQRQLGSSARFVYEACGARTFVQRFRLQYLLGSHAGSVSTIHFNQRGTR
  LASSGDDLRVIVWDWVRQKPVLNFESGHDINVIQAKFFPNCGDSTLAMCGHDGQVRVAELINASYCENTK
  RVAKHRGPAHELALEPDSPYKFLTSGEDAVVFTIDLRQDRPASKVVVTRENDKKVGLYTISMNPANIYQF
  AVGGHDQFVRIYDQRRIDKKENNGVLKKFTPHHLVYCDFPTNITCVVYSHDGTELLASYNDEDIYLFNSS
  LSDGAQYVKRYKGHRNNDTIKCVNFYGPRSEFVVSGSDCGHVFFWEKSSSQIIQFMEGDRGDIVNCLEPH
  PYLPVLATSGLDQHVRIWTPTAKTATELTGLKDVIKKNKQERDEDNLNYTDSFDNRMLRFFVRHLLQRAH
  QPGWRDHGAEFPDEEELDESSSTSDTSEEEGQDRVQCIPS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001017930
• ORF Size: 1800 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001017930.2
• RefSeq Size: 3457 bp
• RefSeq ORF: 1803 bp
• Locus ID: 139425
• UniProt ID: A6NGE4
• Cytogenetics: Xp21.3
• MW: 67.2 kDa
• Gene Summary: This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by Gly-His and Trp-Asp (GH-WD), which may facilitate the formation of heterotrimeric or multi-protein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene appears to represent an intronless retrocopy of a related multi-exon gene located on chromosome 1. However, the CDS of this intronless gene remains intact, it is conserved in other primate species, it is known to be transcribed, and it is therefore thought to encode a functional protein. [provided by RefSeq, May 2010]