NBPF14 (NM_015383) Human Tagged ORF Clone

CAT#: RC220508

NBPF14 (Myc-DDK-tagged)-Human neuroblastoma breakpoint family, member 14 (NBPF14)

Product Images

Circular map for RC220508

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NBPF14
• Synonyms: DJ328E19.C1.1; NBPF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220508 representing NM_015383
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAGGAATGAGCGACAGTTCAAGGAGGAGAAGCTTGCAGAGCAGCTCAAGCAAGCTGAGGAGCTCA
  GGCAATATAAAGTCCTGGTTCACGCTCAGGAACGAGAGCTGACCCAGTTAAGGGAGAAGTTGCGGGAAGG
  GAGAGATGCCTCCCGCTCATTGAATGAGCATCTCCAGGCCCTCCTCACTCCGGATGAGCCGGACAAGTCC
  CAGGGGCAGGACCTCCAAGAACAGCTGGCTGAGGGGTGTAGACTGGCACAGCACCTTGTCCAAAAGCTCA
  GCCCAGAAAATGACAACGATGACGATGAAGATGTTCAAGTTGAGGTGGCTGAGAAAGTGCAGAAATCGTC
  TGCCCCCAGGGAGATGCAGAAGGCTGAAGAAAAGGAAGTCCCTGAGGACTCACTGGAGGAATGTGCCATC
  ACTTGTTCAAATAGCCATGGCCCTTATGACTCCAACCAGCCACATAGGAAAACCAAAATCACATTTGAGG
  AAGACAAAGTCGACTCAACTCTCATTGGCTCATCCTCTCATGTTGAATGGGAGGATGCTGTACACATTAT
  TCCAGAAAATGAAAGTGATGATGAGGAAGAGGAAGAAAAAGGACCAGTGTCTCCCAGGAATCTGCAGGAG
  TCTGAAGAGGAGGAAGTCCCCCAGGAGTCCTGGGATGAAGGTTATTCGACTCTCTCAATTCCTCCTGAAA
  TGTTGGCCTCGTACAAGTCTTACAGCAGCACATTTCACTCATTAGAGGAACAGCAAGTCTGCATGGCTGT
  TGACATAGGCAGACATCGGTGGGATCAAGTGAAAAAGGAGGACCACGAGGCAACAGGTCCCAGGCTCAGC
  AGAGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTT
  CAGGTTGTCTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTACGTATTGGAGCAACAGCG
  TGTTGGCTTGGCTGTTAACATGGATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCATCA
  TGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTGCAGGACTCACTGGGTAGAT
  GTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTC
  ATTGGAGGAACAGTACCTTGGCTTGGCTCTTGACGTGGACAGAATTAAAAAGGACCAAGAAGAGGAAGAA
  GACCAAGGCCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGG
  ACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGG
  AAGTTCCTTTTATGCATTGGAGGAAAAGCATGTTGGCTTTTCTCTTGACGTGGGAGAAATTGAAAAGAAG
  GGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAG
  AAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGCA
  GGACTCACTGGATAGATGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCCCTAC
  AGAAGTGCCTTTTACATATTGGAGCAACAGCGTGTTGGCTTGGCTGTTGACATGGATGAAATTGAAAAGT
  ACCAAGAAGTGGAAGAAGACCAAGACCCATCATGCCCCAGGCTCAGCGGGGAGCTGTTGGATGAGAAAGA
  GCCTGAAGTCTTGCAGGAGTCACTGGATAGATGCTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGAC
  TCATGCCAGCCCTACAGAAGTGCCTTTTACATATTGGAGCAACAGCGTGTTGGCTTGGCTGTTGACATGG
  ATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCT
  GCTGGATGAGAAAGAGCCTGAAGTCTTGCAGGACTCACTGGGTAGATGTTATTCGACTCCTTCAGGTTAT
  CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATTGGAGGAACAGTACCTTGGCT
  TGGCTCTTGACGTGGACAGAATTAAAAAGGACCAAGAAGAGGAAGAAGACCAAGGCCCACCATGCCCCAG
  GCTCAGCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCA
  ACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATTGGAGG
  AAAAACATGTTGGCTTTTCTCTTGACGTGGGAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGG
  AAGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCC
  AGGCTCAACAGCATGCTGATGGAAGTGGAAGAGCCTGAAGTCTTGCAGGACTCACTGGATATATGTTATT
  CGACTCCGTCAATGTACTTTGAACTACCTGACTCATTCCAGCACTACAGAAGTGTGTTTTACTCATTTGA
  GGAAGAGCATATCAGCTTCGCCCTTTACGTGGACAATAGGTTTTTTACTTTGACGGTGACAAGTCTCCAC
  CTGGTGTTCCAGATGGGAGTCATATTCCCACAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220508 representing NM_015383
  Red=Cloning site Green=Tags(s)
  
  MLRNERQFKEEKLAEQLKQAEELRQYKVLVHAQERELTQLREKLREGRDASRSLNEHLQALLTPDEPDKS
  QGQDLQEQLAEGCRLAQHLVQKLSPENDNDDDEDVQVEVAEKVQKSSAPREMQKAEEKEVPEDSLEECAI
  TCSNSHGPYDSNQPHRKTKITFEEDKVDSTLIGSSSHVEWEDAVHIIPENESDDEEEEEKGPVSPRNLQE
  SEEEEVPQESWDEGYSTLSIPPEMLASYKSYSSTFHSLEEQQVCMAVDIGRHRWDQVKKEDHEATGPRLS
  RELLDEKGPEVLQDSLDRCYSTPSGCLELTDSCQPYRSAFYVLEQQRVGLAVNMDEIEKYQEVEEDQDPS
  CPRLSRELLDEKEPEVLQDSLGRCYSTPSGYLELPDLGQPYSSAVYSLEEQYLGLALDVDRIKKDQEEEE
  DQGPPCPRLSRELLEVVEPEVLQDSLDRCYSTPSSCLEQPDSCQPYGSSFYALEEKHVGFSLDVGEIEKK
  GKGKKRRGRRSKKERRRGRKEGEEDQNPPCPRLSRELLDEKGPEVLQDSLDRCYSTPSGCLELTDSCQPY
  RSAFYILEQQRVGLAVDMDEIEKYQEVEEDQDPSCPRLSGELLDEKEPEVLQESLDRCYSTPSGCLELTD
  SCQPYRSAFYILEQQRVGLAVDMDEIEKYQEVEEDQDPSCPRLSRELLDEKEPEVLQDSLGRCYSTPSGY
  LELPDLGQPYSSAVYSLEEQYLGLALDVDRIKKDQEEEEDQGPPCPRLSRELLEVVEPEVLQDSLDRCYS
  TPSSCLEQPDSCQPYGSSFYALEEKHVGFSLDVGEIEKKGKGKKRRGRRSKKERRRGRKEGEEDQNPPCP
  RLNSMLMEVEEPEVLQDSLDICYSTPSMYFELPDSFQHYRSVFYSFEEEHISFALYVDNRFFTLTVTSLH
  LVFQMGVIFPQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015383
• ORF Size: 2763 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015383.1, NP_056198.1
• RefSeq Size: 3689 bp
• RefSeq ORF: 8460 bp
• Locus ID: 25832
• Cytogenetics: 1q21.2
• MW: 105.7 kDa
• Gene Summary: This gene is a member of the neuroblastoma breakpoint family (NBPF) which consists of dozens of recently duplicated genes primarily located in segmental duplications on human chromosome 1. This gene family has experienced its greatest expansion within the human lineage and has expanded, to a lesser extent, among primates in general. Members of this gene family are characterized by tandemly repeated copies of DUF1220 protein domains. Gene copy number variations in the human chromosomal region 1q21.1, where most DUF1220 domains are located, have been implicated in a number of developmental and neurogenetic diseases such as microcephaly, macrocephaly, autism, schizophrenia, cognitive disability, congenital heart disease, neuroblastoma, and congenital kidney and urinary tract anomalies. Altered expression of some gene family members is associated with several types of cancer. This gene family contains numerous pseudogenes. [provided by RefSeq, May 2013]