PARP4 (NM_006437) Human Tagged ORF Clone

CAT#: RC220444

PARP4 (Myc-DDK-tagged)-Human poly (ADP-ribose) polymerase family, member 4 (PARP4)

Product Images

Circular map for RC220444

• 

  Western blot validation of overexpression lysate (Cat# LY416645) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220444 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PARP4
• Synonyms: ADPRTL1; ARTD4; p193; PARP-4; PARPL; PH5P; VAULT3; VPARP; VWA5C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220444 representing NM_006437
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGATGGGAATCTTTGCAAATTGTATCTTCTGTTTGAAAGTGAAGTACTTACCTCAGCAGCAGAAGA
  AAAAGCTACAAACTGACATTAAGGAAAATGGCGGAAAGTTTTCCTTTTCGTTAAATCCTCAGTGCACACA
  TATAATCTTAGATAATGCTGATGTTCTGAGTCAGTACCAACTGAATTCTATCCAAAAGAACCACGTTCAT
  ATTGCAAACCCAGATTTTATATGGAAATCTATCAGAGAAAAGAGACTCTTGGATGTAAAGAATTATGATC
  CTTATAAGCCCCTGGACATCACACCACCTCCTGATCAGAAGGCGAGCAGTTCTGAAGTGAAAACAGAAGG
  TCTATGCCCGGACAGTGCCACAGAGGAGGAAGACACTGTGGAACTCACTGAGTTTGGTATGCAGAATGTT
  GAAATTCCTCATCTTCCTCAAGATTTTGAAGTTGCAAAATATAACACCTTGGAGAAAGTGGGAATGGAGG
  GAGGCCAGGAAGCTGTGGTGGTGGAGCTTCAGTGTTCGCGGGACTCCAGGGACTGTCCTTTCCTGATATC
  CTCACACTTCCTCCTGGATGATGGCATGGAGACTAGAAGACAGTTTGCTATAAAGAAAACCTCTGAAGAT
  GCAAGTGAATACTTTGAAAATTACATTGAAGAACTGAAGAAACAAGGATTTCTACTAAGAGAACATTTCA
  CACCTGAAGCAACCCAATTAGCATCTGAACAATTGCAAGCATTGCTTTTGGAGGAAGTCATGAATTCAAG
  CACTCTGAGCCAAGAGGTGAGCGATTTAGTAGAGATGATTTGGGCAGAGGCCCTGGGCCACCTGGAACAC
  ATGCTTCTCAAGCCAGTGAACAGGATTAGCCTCAACGATGTGAGCAAGGCAGAGGGGATTCTCCTTCTAG
  TAAAGGCAGCACTGAAAAATGGAGAAACAGCAGAGCAATTGCAAAAGATGATGACAGAGTTTTACAGACT
  GATACCTCACAAAGGCACAATGCCCAAAGAAGTGAACCTGGGACTATTGGCTAAGAAAGCAGACCTCTGC
  CAGCTAATAAGAGACATGGTTAATGTCTGTGAAACTAATTTGTCCAAACCCAACCCACCATCCCTGGCCA
  AATACCGAGCTTTGAGGTGCAAAATTGAGCATGTTGAACAGAATACTGAAGAATTTCTCAGGGTTAGAAA
  AGAGGTTTTGCAGAATCATCACAGTAAGAGCCCAGTGGATGTCTTGCAGATATTTAGAGTTGGCAGAGTG
  AATGAAACCACAGAGTTTTTGAGCAAACTTGGTAATGTGAGGCCCTTGTTGCATGGTTCTCCTGTACAAA
  ACATCGTGGGAATCTTGTGTCGAGGGTTGCTTTTACCCAAAGTAGTGGAAGATCGTGGTGTGCAAAGAAC
  AGACGTCGGAAACCTTGGAAGTGGGATTTATTTCAGTGATTCGCTCAGTACAAGTATCAAGTACTCACAC
  CCGGGAGAGACAGATGGCACCAGACTCCTGCTCATTTGTGACGTAGCCCTCGGAAAGTGTATGGACTTAC
  ATGAGAAGGACTTTTCCTTAACTGAAGCACCACCAGGCTACGACAGTGTGCATGGAGTTTCACAAACAGC
  CTCTGTCACCACAGACTTTGAGGATGATGAATTTGTTGTCTATAAAACCAATCAGGTTAAAATGAAATAT
  ATTATTAAATTTTCCATGCCTGGAGATCAGATAAAGGACTTTCATCCTAGTGATCATACTGAATTAGAGG
  AATACAGACCTGAGTTTTCAAATTTTTCAAAGGTTGAAGATTACCAGTTACCAGATGCCAAAACTTCCAG
  CAGCACCAAGGCCGGCCTCCAGGATGCCTCTGGGAACTTGGTTCCTCTGGAGGATGTCCACATCAAAGGG
  AGAATCATAGACACTGTAGCCCAGGTCATTGTTTTTCAGACATACACAAATAAAAGTCACGTGCCCATTG
  AGGCAAAATATATCTTTCCTTTGGATGACAAGGCCGCTGTGTGTGGCTTCGAAGCCTTCATCAATGGGAA
  GCACATAGTTGGAGAGATTAAAGAGAAGGAAGAAGCCCAGCAAGAGTACCTAGAAGCCGTGACCCAGGGC
  CATGGCGCTTACCTGATGAGTCAGGATGCTCCGGACGTTTTTACTGTAAGTGTTGGAAACTTACCCCCTA
  AGGCTAAGGTTCTTATAAAAATTACCTACATCACAGAACTCAGCATCCTGGGCACTGTTGGTGTCTTTTT
  CATGCCCGCCACCGTAGCACCCTGGCAACAGGACAAGGCTTTGAATGAAAACCTTCAGGATACAGTAGAG
  AAGATTTGTATAAAAGAAATAGGAACAAAGCAAAGCTTCTCTTTGACTATGTCTATTGAGATGCCGTATG
  TGATTGAATTCATTTTCAGTGATACACATGAACTGAAACAAAAGCGCACAGACTGCAAAGCTGTCATTAG
  CACCATGGAAGGCAGCTCCTTAGACAGCAGTGGATTTTCTCTCCACATCGGTTTGTCTGCTGCCTATCTC
  CCAAGAATGTGGGTTGAAAAACATCCAGAAAAAGAAAGCGAGGCTTGCATGCTTGTCTTTCAACCCGATC
  TCGATGTCGACCTCCCTGACCTAGCCAGTGAGAGCGAAGTGATTATTTGTCTTGACTGCTCCAGTTCCAT
  GGAGGGTGTGACATTCTTGCAAGCCAAGCAAATCACCTTGCATGCGCTGTCCTTGGTGGGTGAGAAGCAG
  AAAGTAAATATTATCCAGTTCGGCACAGGTTACAAGGAGCTATTTTCGTATCCTAAGCATATCACAAGCA
  ATACCACGGCAGCAGAGTTCATCATGTCTGCCACACCTACCATGGGGAACACAGACTTCTGGAAAACACT
  CCGATATCTTAGCTTATTGTACCCTGCTCGAGGGTCACGGAACATCCTCCTGGTGTCTGATGGGCACCTC
  CAGGATGAGAGCCTGACATTACAGCTCGTGAAGAGGAGCCGCCCGCACACCAGGTTATTCGCCTGCGGTA
  TCGGTTCTACAGCAAATCGTCACGTCTTAAGGATTTTGTCCCAGTGTGGTGCCGGAGTATTTGAATATTT
  TAATGCAAAATCCAAGCATAGTTGGAGAAAACAGATAGAAGACCAAATGACCAGGCTATGTTCTCCGAGT
  TGCCACTCTGTCTCCGTCAAATGGCAGCAACTCAATCCAGATGCGCCCGAGGCCCTGCAGGCCCCAGCCC
  AGGTGCCATCCTTGTTTCGCAATGATCGACTCCTTGTCTATGGATTCATTCCTCACTGCACACAAGCAAC
  TCTGTGTGCACTAATTCAAGAGAAAGAATTTTGTACAATGGTGTCGACTACTGAGCTTCAGAAGACAACT
  GGAACTATGATCCACAAGCTGGCAGCCCGAGCTCTAATCAGAGATTATGAAGATGGCATTCTTCACGAAA
  ATGAAACCAGTCATGAGATGAAAAAACAAACCTTGAAATCTCTGATTATTAAACTCAGTAAAGAAAACTC
  TCTCATAACACAATTTACAAGCTTTGTGGCAGTTGAGAAAAGGGATGAGAATGAGTCGCCTTTTCCTGAT
  ATTCCAAAAGTTTCTGAACTTATTGCCAAAGAAGATGTAGACTTCCTGCCCTACATGAGCTGGCAGGGGG
  AGCCCCAAGAAGCCGTCAGGAACCAGTCTCTTTTAGCATCCTCTGAGTGGCCAGAATTACGTTTATCCAA
  ACGAAAACATAGGAAAATTCCATTTTCCAAAAGAAAAATGGAATTATCTCAGCCAGAAGTTTCTGAAGAT
  TTTGAAGAGGATGGCTTAGGTGTACTACCAGCTTTCACATCAAATTTGGAACGTGGAGGTGTGGAAAAGC
  TATTGGATTTAAGTTGGACAGAGTCATGTAAACCAACAGCAACTGAACCACTATTTAAGAAAGTCAGTCC
  ATGGGAAACATCTACTTCTAGCTTTTTTCCTATTTTGGCTCCGGCCGTTGGTTCCTATCTTCCCCCGACT
  GCCCGCGCTCACAGTCCTGCTTCCTTGTCTTTTGCCTCATATCGTCAGGTAGCTAGTTTCGGTTCAGCTG
  CTCCTCCCAGACAGTTTGATGCATCTCAATTCAGCCAAGGCCCTGTGCCTGGCACTTGTGCTGACTGGAT
  CCCACAGTCGGCGTCTTGTCCCACAGGACCTCCCCAGAACCCACCTTCTGCACCCTATTGTGGCATTGTT
  TTTTCAGGGAGCTCATTAAGCTCTGCACAGTCTGCTCCACTGCAACATCCTGGAGGCTTTACTACCAGGC
  CTTCTGCTGGCACCTTCCCTGAGCTGGATTCTCCCCAGCTTCATTTCTCTCTTCCTACAGACCCTGATCC
  CATCAGAGGTTTTGGGTCTTATCATCCCTCTGCTTACTCTCCTTTTCATTTTCAACCTTCCGCAGCCTCT
  TTGACTGCCAACCTTAGGCTGCCAATGGCCTCTGCTTTACCTGAGGCTCTTTGCAGTCAGTCCCGGACTA
  CCCCAGTAGATCTCTGTCTTCTAGAAGAATCAGTAGGCAGTCTCGAAGGAAGTCGATGTCCTGTCTTTGC
  TTTTCAAAGTTCTGACACAGAAAGTGATGAGCTATCAGAAGTACTTCAAGACAGCTGCTTTTTACAAATA
  AAATGTGATACAAAAGATGACAGTATCCCGTGCTTTCTGGAAGTAAAAGAAGAGGATGAAATAGTGTGCA
  CACAACACTGGCAGGATGCTGTGCCTTGGACAGAACTCCTCAGTCTACAGACAGAGGATGGCTTCTGGAA
  ACTTACACCAGAACTGGGACTTATATTAAATCTTAATACAAATGGTTTGCACAGCTTTCTTAAACAAAAA
  GGCATTCAATCTCTAGGTGTAAAAGGAAGAGAATGTCTCCTGGACCTAATTGCCACAATGCTGGTACTAC
  AGTTTATTCGCACCAGGTTGGAAAAAGAGGGAATAGTGTTCAAATCACTGATGAAAATGGATGACGCTTC
  TATTTCCAGGAATATTCCCTGGGCTTTTGAGGCAATAAAGCAAGCAAGTGAATGGGTAAGAAGAACTGAA
  GGACAGTACCCATCTATCTGCCCACGGCTTGAACTGGGGAACGACTGGGACTCTGCCACCAAGCAGTTGC
  TGGGACTCCAGCCCATAAGCACTGTGTCCCCTCTTCATAGAGTCCTCCATTACAGTCAAGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220444 representing NM_006437
  Red=Cloning site Green=Tags(s)
  
  MVMGIFANCIFCLKVKYLPQQQKKKLQTDIKENGGKFSFSLNPQCTHIILDNADVLSQYQLNSIQKNHVH
  IANPDFIWKSIREKRLLDVKNYDPYKPLDITPPPDQKASSSEVKTEGLCPDSATEEEDTVELTEFGMQNV
  EIPHLPQDFEVAKYNTLEKVGMEGGQEAVVVELQCSRDSRDCPFLISSHFLLDDGMETRRQFAIKKTSED
  ASEYFENYIEELKKQGFLLREHFTPEATQLASEQLQALLLEEVMNSSTLSQEVSDLVEMIWAEALGHLEH
  MLLKPVNRISLNDVSKAEGILLLVKAALKNGETAEQLQKMMTEFYRLIPHKGTMPKEVNLGLLAKKADLC
  QLIRDMVNVCETNLSKPNPPSLAKYRALRCKIEHVEQNTEEFLRVRKEVLQNHHSKSPVDVLQIFRVGRV
  NETTEFLSKLGNVRPLLHGSPVQNIVGILCRGLLLPKVVEDRGVQRTDVGNLGSGIYFSDSLSTSIKYSH
  PGETDGTRLLLICDVALGKCMDLHEKDFSLTEAPPGYDSVHGVSQTASVTTDFEDDEFVVYKTNQVKMKY
  IIKFSMPGDQIKDFHPSDHTELEEYRPEFSNFSKVEDYQLPDAKTSSSTKAGLQDASGNLVPLEDVHIKG
  RIIDTVAQVIVFQTYTNKSHVPIEAKYIFPLDDKAAVCGFEAFINGKHIVGEIKEKEEAQQEYLEAVTQG
  HGAYLMSQDAPDVFTVSVGNLPPKAKVLIKITYITELSILGTVGVFFMPATVAPWQQDKALNENLQDTVE
  KICIKEIGTKQSFSLTMSIEMPYVIEFIFSDTHELKQKRTDCKAVISTMEGSSLDSSGFSLHIGLSAAYL
  PRMWVEKHPEKESEACMLVFQPDLDVDLPDLASESEVIICLDCSSSMEGVTFLQAKQITLHALSLVGEKQ
  KVNIIQFGTGYKELFSYPKHITSNTTAAEFIMSATPTMGNTDFWKTLRYLSLLYPARGSRNILLVSDGHL
  QDESLTLQLVKRSRPHTRLFACGIGSTANRHVLRILSQCGAGVFEYFNAKSKHSWRKQIEDQMTRLCSPS
  CHSVSVKWQQLNPDAPEALQAPAQVPSLFRNDRLLVYGFIPHCTQATLCALIQEKEFCTMVSTTELQKTT
  GTMIHKLAARALIRDYEDGILHENETSHEMKKQTLKSLIIKLSKENSLITQFTSFVAVEKRDENESPFPD
  IPKVSELIAKEDVDFLPYMSWQGEPQEAVRNQSLLASSEWPELRLSKRKHRKIPFSKRKMELSQPEVSED
  FEEDGLGVLPAFTSNLERGGVEKLLDLSWTESCKPTATEPLFKKVSPWETSTSSFFPILAPAVGSYLPPT
  ARAHSPASLSFASYRQVASFGSAAPPRQFDASQFSQGPVPGTCADWIPQSASCPTGPPQNPPSAPYCGIV
  FSGSSLSSAQSAPLQHPGGFTTRPSAGTFPELDSPQLHFSLPTDPDPIRGFGSYHPSAYSPFHFQPSAAS
  LTANLRLPMASALPEALCSQSRTTPVDLCLLEESVGSLEGSRCPVFAFQSSDTESDELSEVLQDSCFLQI
  KCDTKDDSIPCFLEVKEEDEIVCTQHWQDAVPWTELLSLQTEDGFWKLTPELGLILNLNTNGLHSFLKQK
  GIQSLGVKGRECLLDLIATMLVLQFIRTRLEKEGIVFKSLMKMDDASISRNIPWAFEAIKQASEWVRRTE
  GQYPSICPRLELGNDWDSATKQLLGLQPISTVSPLHRVLHYSQG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006437
• ORF Size: 5172 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 5468 bp
• RefSeq ORF: 5175 bp
• Locus ID: 143
• UniProt ID: Q9UKK3
• Cytogenetics: 13q12.12
• Domains: PARP, BRCT, VWA, VIT
• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair
• MW: 192.4 kDa
• Gene Summary: This gene encodes poly(ADP-ribosyl)transferase-like 1 protein, which is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. Since this protein is not capable of binding DNA directly, its transferase activity may be activated by other factors such as protein-protein interaction mediated by the extensive carboxyl terminus. [provided by RefSeq, Jul 2008]