PARP4 (NM_006437) Human Untagged Clone

CAT#: SC116119

PARP4 (untagged)-Human poly (ADP-ribose) polymerase family, member 4 (PARP4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PARP4
• Synonyms: ADPRTL1; ARTD4; p193; PARP-4; PARPL; PH5P; VAULT3; VPARP; VWA5C
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006437 edited
  ACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGAAGTACCGCGAGCAAGGAGCGCGGAA
  TCGGGGAGCGTCCGGAGCTAGCTGGATCCTCTAGGCAGGATGGTGATGGGAATCTTTGCA
  AATTGTATCTTCTGTTTGAAAGTGAAGTACTTACCTCAGCAGCAGAAGAAAAAGCTACAA
  ACTGACATTAAGGAAAATGGCGGAAAGTTTTCCTTTTCGTTAAATCCTCAGTGCACACAT
  ATAATCTTAGATAATGCTGATGTTCTGAGTCAGTACCAACTGAATTCTATCCAAAAGAAC
  CACGTTCATATTGCAAACCCAGATTTTATATGGAAATCTATCAGGGAAAAGAGACTCTTG
  GATGTAAAGAATTATGATCCTTATAAGCCCCTGGACATCACACCACCTCCTGATCAGAAG
  GCGAGCAGTTCTGAAGTGAAAACAGAAGGTCTATGCCCGGACAGTGCCACAGAGGAGGAA
  GACACTGTGGAACTCACTGAGTTTGGTATGCAGAATGTTGAAATTCCTCATCTTCCTCAA
  GATTTTGAAGTTGCAAAATATAACACCTTGGAGAAAGTGGGAATGGAGGGAGGCCAGGAA
  GCTGTGGTGGTGGAGCTTCAGTGTTCGCGGGACTCCAGGGACTGTCCTTTCCTGATATCC
  TCACACTTCCTCCTGGATGATGGCATGGAGACTAGAAGACAGTTTGCTATAAAGAAAACC
  TCTGAAGATGCAAGTGAATACTTTGAAAATTACATTGAAGAACTGAAGAAACAAGGATTT
  CTACTAAGAGAACATTTCACACCTGAAGCAACCCAATTAGCATCTGAACAATTGCAAGCA
  TTGCTTTTGGAGGAAGTCATGAATTCAAGCACTCTGAGCCAAGAGGTGAGCGATTTAGTA
  GAGATGATTTGGGCAGAGGCCCTGGGCCACCTGGAACACATGCTTCTCAAGCCAGTGAAC
  AGGATTAGCCTCAACGATGTGAGCAAGGCAGAGGGGATTCTCCTTCTAGTAAAGGCAGCA
  CTGAAAAATGGAGAAACAGCAGAGCAATTGCAAAAGATGATGACAGAGTTTTACAGACTG
  ATACCTCACAAAGGCACAATGCCCAAAGAAGTGAACCTGGGACTATTGGCTAAGAAAGCA
  GACCTCTGCCAGCTAATAAGAGACATGGTTAATGTCTGTGAAACTAATTTGTCCAAACCC
  AACCCACCATCCCTGGCCAAATACCGAGCTTTGAGGTGCAAAATTGAGCATGTTGAACAG
  AATACTGAAGAATTTCTCAGGGTTAGAAAAGAGGTTTTGCAGAATCATCACAGTAAGAGC
  CCAGTGGATGTCTTGCAGATATTTAGAGTTGGCAGAGTGAATGAAACCACAGAGTTTTTG
  AGCAAACTTGGTAATGTGAGGCCCTTGTTGCATGGTTCTCCTGTACAAAACATCGTGGGA
  ATCTTGTGTCGAGGGTTGCTTTTACCCAAAGTAGTGGAAGATCGTGGTGTGCAAAGAACA
  GACGTCGGAAACCTTGGAAGTGGGATTTATTTCAGTGATTCGCTCAGTACAAGTATCAAG
  TACTCACACCCGGGAGAGACAGATGGCACCAGACTCCTGCTCATTTGTGACGTAGCCCTC
  GGAAAGTGTATGGACTTACATGAGAAGGACTTTTCCTTAACTGAAGCACCACCAGGCTAC
  GACAGTGTGCATGGAGTTTCGCAAACAGCCTCTGTCACCACAGACTTTGAGGATGATGAA
  TTTGTTGTCTATAAAACCAATCAGGTTAAAATGAAATATATTATTAAATTTTCCATGCCT
  GGAGATCAGATAAAGGACTTTCATCCTAGTGATCATACTGAATTAGAGGAATACAGACCT
  GAGTTTTCAAATTTTTCAAAGGTTGAAGATTACCAGTTACCAGATGCCAAAACTTCCAGC
  AGCACCAAGGCCGGCCTCCAGGATGCCTCTGGGAACTTGGTTCCTCTGGAGGATGTCCAC
  ATCAAAGGGAGAATCATAGACACTGTAGCCCAGGTCATTGTTTTTCAGACATACACAAAT
  AAAAGTCACGTGCCCATTGAGGCAAAATATATCTTTCCTTTGGATGACAAGGCCGCTGTG
  TGTGGCTTCGAAGCCTTCATCAATGGGAAGCACATAGTTGGAGAGATTAAAGAGAAGGAA
  GAAGCCCAGCAAGAGTACCTAGAAGCCGTGACCCAGGGCCATGGCGCTTACCTGATGAGT
  CAGGATGCTCCGGACGTTTTTACTGTAAGTGTTGGAAACTTACCCCCTAAGGCTAAGGTT
  CTTATAAAAATTACCTACATCACAGAACTCAGCATCCTGGGCACTGTTGGTGTCTTTTTC
  ATGCCCGCCACCGTAGCACCCTGGCAACAGGACAAGGCTTTGAATGAAAACCTTCAGGAT
  ACAGTAGAGAAGATTTGTATAAAAGAAATAGGAACAAAGCAAAGCTTCTCTTTGACTATG
  TCTATTGAGATGCCGTATGTGATTGAATTCATTTTCAGTGATACACATGAACTGAAACAA
  AAGCGCACAGACTGCAAAGCTGTCATTAGCACCATGGAAGGCAGCTCCTTAGACAGCAGT
  GGATTTTCTCTCCACATCGGTTTGTCTGCTGCCTATCTCCCAAGAATGTGGGTTGAAAAA
  CATCCAGAAAAAGAAAGCGAGGCTTGCATGCTTGTCTTTCAACCCGATCTCGATGTCGAC
  CTCCCTGACCTAGCCAGTGAGAGCGAAGTGATTATTTGTCTTGACTGCTCCAGTTCCATG
  GAGGGTGTGACATTCTTGCAAGCCAAGCAAATCACCTTGCATGCGCTGTCCTTGGTGGGT
  GAGAAGCAGAAAGTAAATATTATCCAGTTCGGCACAGGTTACAAGGAGCTATTTTCGTAT
  CCTAAGCATATCACAAGCAATACCACGGCAGCAGAGTTCATCATGTCTGCCACACCTACC
  ATGGGGAACACAGACTTCTGGAAAACACTCCGATATCTTAGCTTATTGTACCCTGCTCGA
  GGGTCACGGAACATCCTCCTGGTGTCTGATGGGCACCTCCAGGATGAGAGCCTGACATTA
  CAGCTCGTGAAGAGGAGCCGCCCGCACACCAGGTTATTCGCCTGCGGTATCGGTTCTACA
  GCAAATCGTCACGTCTTAAGGATTTTGTCCCAGTGTGGTGCCGGAGTATTTGAATATTTT
  AATGCAAAATCCAAGCATAGTTGGAGAAAACAGATAGAAGACCAAATGACCAGGCTATGT
  TCTCCGAGTTGCCACTCTGTCTCCGTCAAATGGCAGCAACTCAATCCAGATGCGCCCGAG
  GCCCTGCAGGCCCCAGCCCAGGTGCCATCCTTGTTTCGCAATGATCGACTCCTTGTCTAT
  GGATTCATTCCTCACTGCACACAGGCAACTCTGTGTGCACTAATTCAAGAGAAAGAATTT
  TGTACAATGGTGTCGACTACTGAGCTTCAGAAGACAACTGGAACTATGATCCACAAGCTG
  GCAGCCCGAGCTCTAATCAGAGATTATGAAGATGGCATTCTTCACGAAAATGAAACCAGT
  CATGAGATGAAAAAACAAACCTTGAAATCTCTGATTATTAAACTCAGTAAAGAAAACTCT
  CTCATAACACAATTTACAAGCTTTGTGGCAGTTGAGAAAAGGGATGAGAATGAGTCACCT
  TTTCCTGATATTCCAAAAGTTTCTGAACTTATTGCCAAAGAAGATGTAGACTTCCTGCCC
  TACATGAGCTGGCAGGGGGAACCCCAAGAAGCCGTCAGGAACCAGTCTCTTTTAGCATCC
  TCTGAGTGGCCAGAATTACGTTTATCCAAACGAAAACATAGGAAAATTCCATTTTCCAAA
  AGAAAAATGGAATTATCTCAGCCAGAAGTTTCTGAAGATTTTGAAGAGGATGCCTTAGGT
  GTACTACCAGCTTTCACATCAAATTTGGAACGTGGACGTGTGGAAAAGCTATTGGATTTA
  AGTTGGACAGAGTCATGTAAACCAACAGCAACTGAACCACTATTTAAGAAAGTCAGTCCA
  TGGGAAACATCTACTTCTAGCTTTTTTCCTATTTTGGCTCCGGCCGTTGGTTCCTATCTT
  ACCCCGACTGCCCGCGCTCACAGTCCTGCTTCCTTGTCTTTTGCCTCATATCGTCAGGTA
  GCTAGTTTCGGTTCAGCTGCTCCTCCCAGACAGTTTGATGCATCTCAATTCAGCCAAGGC
  CCTGTGCCTGGCACTTGTGCTGACTGGATCCCACAGTCGGCGTCTTGTCCCACAGGACCT
  CCCCAGAACCCACCTTCTGCACCCTATTGTGGCATTGTTTTTTCAGGGAGCTCATTAAGC
  TCTGCACAGTCTGCTCCACTGCAACATCCTGGAGGCTTTACTACCAGGCCTTCTGCTGGC
  ACCTTCCCTGAGCTGGATTCTCCCCAGCTTCATTTCTCTCTTCCTACAGACCCTGATCCC
  ATCAGAGGTTTTGGGTCTTATCATCCCTCTGCTTACTCTCCTTTTCATTTTCAACCTTCC
  GCAGCCTCTTTGACTGCCAACCTTAGGCTGCCAATGGCCTCTGCTTTACCTGAGGCTCTT
  TGCAGTCAGTCCCGGACTACCCCAGTAGATCTCTGTCTTCTAGAAGAATCAGTAGGCAGT
  CTCGAAGGAAGTCGATGTCCTGTCTTTGCTTTTCAAAGTTCTGACACAGAAAGTGATGAG
  CTATCAGAAGTACTTCAAGACAGCTGCTTTTTACAAATAAAATGTGATACAAAAGATGAC
  AGTATCCCGTGCTTTCTGGAAGTAAAAGAAGAGGATGAAATAGTGTGCACACAACACTGG
  CAGGATGCTGTGCCTTGGACAGAACTCCTCAGTCTACAGACAGAGGATGGCTTCTGGAAA
  CTTACACCAGAACTGGGACTTATATTAAATCTTAATACAAATGGTTTGCACAGCTTTCTT
  AAACAAAAAGGCATTCAATCTCTAGGTGTAAAAGGAAGAGAATGTCTCCTGGACCTAATT
  GCCACAATGCTGGTACTACAGTTTATTCGCACCAGGTTGGAAAAAGAGGGAATAGTGTTC
  AAATCACTGATGAAAATGGATGACCCTTCTATTTCCAGGAATATTCCCTGGGCTTTTGAG
  GCAATAAAGCAAGCAAGTGAATGGGTAAGAAGAACTGAAGGACAGTACCCATCTATCTGC
  CCACGGCTTGAACTGGGGAACGACTGGGACTCTGCCACCAAGCAGTTGCTGGGACTCCAG
  CCCATAAGCACTGTGTCCCCTCTTCATAGAGTCCTCCATTACAGTCAAGGCTAAGTCAAA
  TGAAACTGAATTTTAAACTTTTTGCATGCTTCTATGTAGAAAATAATCAAATGATAATAG
  ATACTTATAATGAAACTTCATTAAGGTTTCATTC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006437 unedited
  GGATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGGAGTACC
  GCGAGCAAGGAGCGCGGAATCGGGGAGCGTCCGGAGCTAGCTGGATCCTCTAGGCAGGAT
  GGTGATGGGAATCTTTGCAAATTGTATCTTCTGTTTGAAAGTGAAGTACTTACCTCAGCA
  GCAGAAGAAAAAGCTACAAACTGACATTAAGGAAAATGGCGGAAAGTTTTCCTTTTCGTT
  AAATCCTCAGTGCACACATATAATCTTAGATAATGCTGATGTTCTGAGTCAGTACCAACT
  GAATTCTATCCAAAAGAACCACGTTCATATTGCAAACCCAGATTTTATATGGAAATCTAT
  CAGGGAAAAGAGACTCTTGGATGTAAAGAATTATGATCCTTATAAGCCCCTGGACATCAC
  ACCACCTCCTGATCAGAAGGCGAGCAGTTCTGAAGTGAAAACAGAAGGTCTATGCCCGGA
  CAGTGCCACAGAGGAGGAAGACACTGTGGAACTCACTGAGTTTGGTATGCAGAATGTTGA
  AATTCCTCATCTTCCTCAAGATTTTGAAGTTGCAAAATATAACACCTTGGAGAAAGTGGG
  AATGGAGGGAGGCCAGGAAGCTGTGGTGGTGGAGCTTCAGTGTTCGCGGGACTCCAGGGA
  CTGTCCTTTCCTGATATCCTCACACTTCCTCCTGGATGATGGCATGGAGACTAGAAGACA
  GTTTGCTATAAAGAAAACCTCTGAAGATGCAAGTGAATACTTTGAAAAATACATTNGAGA
  AACTGAGAAACANGGATNTCTACNTAGAGAACATTTCACACCTGAAAGCACCCATTAGCA
  TCTGAACAATTNGCAGCATTGCTTTTGGNAGAAGTCATGAATTCAGCACTCTGAGCCCAG
  AGTGAGCGATTANTANAGATGATTTGGCAGAGCCCTGGCCACTGGACACAGCTCTCAGCC
  ATGACAGATAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006437 unedited
  CCGCGGCCGCAATCTAAGTCGAGNNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTATTN
  AAAGNTTCATTTTATTATTGCTTGTTAGTTGATTAAAGAAATTCTTCTTCCACTTAATTT
  TTAAAGACAGTAATTGCTACACTGAATGAAACCTTAATGAAGTTTCATTATAAGTATCTA
  TTATCATTTGATTATTTTCTACATAGAAGCATGCAAAAAGTTTAAAATTCAGTTTCATTT
  GACTTAACCTTGACTGTAATGGAGGACTCTATGAAGAGGGGACACAGTGCTTATGGGCTG
  GAGTCCCAGCAACTGCTTGGTGGCAGAGTCCCAGTCGTTCCCCAGTTCAAGCCGTGGGCA
  GATAGATGGGTACTGTCCTTCAGTTCTTCTTACCCATTCACTTGCTTGCTTTATTGCCTC
  AAAAGCCCAGGGAATATTCCTGGAAATAGAAGGGTCATCCATTTTCATCAGTGATTTGAA
  CACTATTCCCTCTTTTTCCAACCTGGTGCGAATAAACTGTAGTACCAGCATTGTGGCAAT
  TAGGTCCAGGAGACATTCTCTTCCTTTTACACCTAGAGATTGAATGCCTTTTTGTTTAAG
  AAAGCTGTGCAAACCATTTGTATTAAGATTTAATATAAGTCCCAGTTCTGGTGTAAGTTT
  CCAGAAGCCATCCTCTGTCTGTAGACTGAGGAGTTCTGTCCAAGGCACAGCATCCTGCCA
  GTGNTGTGTGCACACTATTTCATCCTCTTCTTTTACTCNCAGAAAGCACGGATACTGNCA
  TCTTTTGTATCACATTTTATTTGTAAAAGCAGCTGTCTTGAGTACTTCTGAAGCTCATAC
  TTTCTGTGCAGAACTTTGAAAGCAAGACAGAATCGACTTNCTCGAGATGNNCTACTGATC
  TCTAGAGACGAGACTACTGNGGTAGTCGGNACTGACTGAAGAGCTCAGTAAGCANAGCCA
  TTGCANCCTAGNTGCAGCAAGAGCTGCGAAGGTGAAATA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006437
• Insert Size: 5650 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006437.2, NP_006428.1
• RefSeq Size: 5468 bp
• RefSeq ORF: 5175 bp
• Locus ID: 143
• UniProt ID: Q9UKK3
• Cytogenetics: 13q12.12
• Domains: PARP, BRCT, VWA, VIT
• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair
• Gene Summary: This gene encodes poly(ADP-ribosyl)transferase-like 1 protein, which is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. Since this protein is not capable of binding DNA directly, its transferase activity may be activated by other factors such as protein-protein interaction mediated by the extensive carboxyl terminus. [provided by RefSeq, Jul 2008]