RPGR (NM_000328) Human Tagged ORF Clone

CAT#: RC220424

RPGR (Myc-DDK-tagged)-Human retinitis pigmentosa GTPase regulator (RPGR), transcript variant A

Product Images

Circular map for RC220424

• 

  Western blot validation of overexpression lysate (Cat# LY424796) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220424 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RPGR
• Synonyms: COD1; CORDX1; CRD; orf15; PCDX; RP3; RP15; XLRP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220424 representing NM_000328
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGAGCCGGAAGAGCTGATGCCCGATTCGGGTGCTGTGTTTACATTTGGGAAAAGTAAATTTGCTG
  AAAATAATCCCGGTAAATTCTGGTTTAAAAATGATGTCCCTGTACATCTTTCATGTGGAGATGAACATTC
  TGCTGTTGTTACCGGAAATAATAAACTTTACATGTTTGGCAGTAACAACTGGGGTCAGTTAGGATTAGGA
  TCAAAGTCAGCCATCAGCAAGCCAACATGTGTCAAAGCTCTAAAACCTGAAAAAGTGAAATTAGCTGCCT
  GTGGAAGGAACCACACCCTGGTGTCAACAGAAGGAGGCAATGTATATGCAACTGGTGGAAATAATGAAGG
  ACAGTTGGGGCTTGGTGACACCGAAGAAAGAAACACTTTTCATGTAATTAGCTTTTTTACATCCGAGCAT
  AAGATTAAGCAGCTGTCTGCTGGATCTAATACTTCAGCTGCCCTAACTGAGGATGGAAGACTTTTTATGT
  GGGGTGACAATTCCGAAGGGCAAATTGGTTTAAAAAATGTAAGTAATGTCTGTGTCCCTCAGCAAGTGAC
  CATTGGGAAACCTGTCTCCTGGATCTCTTGTGGATATTACCATTCAGCTTTTGTAACAACAGATGGTGAG
  CTATATGTGTTTGGAGAACCTGAGAATGGGAAGTTAGGTCTTCCCAATCAGCTCCTGGGCAATCACAGAA
  CACCCCAGCTGGTGTCTGAAATTCCGGAGAAGGTGATCCAAGTAGCCTGTGGTGGAGAGCATACTGTGGT
  TCTCACGGAGAATGCTGTGTATACCTTTGGGCTGGGACAATTTGGTCAGCTGGGTCTTGGCACTTTTCTT
  TTTGAAACTTCAGAACCCAAAGTCATTGAGAATATTAGGGATCAAACAATAAGTTATATTTCTTGTGGAG
  AAAATCACACAGCTTTGATAACAGATATCGGCCTTATGTATACTTTTGGAGATGGTCGCCACGGAAAATT
  AGGACTTGGACTGGAGAATTTTACCAATCACTTCATTCCTACTTTGTGCTCTAATTTTTTGAGGTTTATA
  GTTAAATTGGTTGCTTGTGGTGGATGTCACATGGTAGTTTTTGCTGCTCCTCATCGTGGTGTGGCAAAAG
  AAATTGAATTCGATGAAATAAATGATACTTGCTTATCTGTGGCGACTTTTCTGCCGTATAGCAGTTTAAC
  CTCAGGAAATGTACTGCAGAGGACTCTATCAGCACGTATGCGGCGAAGAGAGAGGGAGAGGTCTCCAGAT
  TCTTTTTCAATGAGGAGAACACTACCTCCAATAGAAGGGACTCTTGGCCTTTCTGCTTGTTTTCTCCCCA
  ATTCAGTCTTTCCACGATGTTCTGAGAGAAACCTCCAAGAGAGTGTCTTATCTGAACAGGACCTCATGCA
  GCCAGAGGAACCAGATTATTTGCTAGATGAAATGACCAAAGAAGCAGAGATAGATAATTCTTCAACTGTA
  GAAAGCCTTGGAGAAACTACTGATATCTTAAACATGACACACATCATGAGCCTGAATTCCAATGAAAAGT
  CATTAAAATTATCACCAGTTCAGAAACAAAAGAAACAACAAACAATTGGGGAACTGACGCAGGATACAGC
  TCTTACTGAAAACGATGATAGTGATGAATATGAAGAAATGTCAGAAATGAAAGAAGGGAAAGCATGTAAA
  CAACATGTGTCACAAGGGATTTTCATGACGCAGCCAGCTACGACTATCGAAGCATTTTCAGATGAGGAAG
  TAGAGATCCCAGAGGAGAAGGAAGGAGCAGAGGATTCAAAAGGAAATGGAATAGAGGAGCAAGAGGTAGA
  AGCAAATGAGGAAAATGTGAAGGTGCATGGAGGAAGAAAGGAGAAAACAGAGATCCTATCAGATGACCTT
  ACAGACAAAGCAGAGGATCATGAATTTTCTAAAACTGAGGAACTAAAACTAGAAGATGTGGATGAGGAAA
  TTAATGCTGAAAATGTGGAAAGCAAGAAGAAAACTGTGGGAGATGATGAAAGTGTTCCTACAGGTTATCA
  CAGTAAAACAGAAGGAGCAGAAAGAACCAATGATGATAGCTCAGCTGAAACTATTGAAAAGAAAGAAAAA
  GCCAACCTAGAGGAACGGGCCATTTGTGAGTACAATGAAAACCCAAAAGGATACATGCTTGATGATGCAG
  ATAGCAGTTCATTAGAAATCCTAGAAAACAGTGAAACAACACCAAGCAAAGACATGAAAAAAACAAAGAA
  GATTTTTCTGTTCAAAAGAGTCCCCTCAATAAATCAAAAGATTGTCAAGAATAACAATGAGCCGCTCCCA
  GAGATAAAATCCATAGGAGACCAGATCATTTTAAAAAGTGATAATAAAGATGCCGACCAGAACCACATGA
  GTCAGAATCATCAGAATATCCCACCAACAAATACAGAGAGAAGATCAAAATCCTGTACAATACTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220424 representing NM_000328
  Red=Cloning site Green=Tags(s)
  
  MREPEELMPDSGAVFTFGKSKFAENNPGKFWFKNDVPVHLSCGDEHSAVVTGNNKLYMFGSNNWGQLGLG
  SKSAISKPTCVKALKPEKVKLAACGRNHTLVSTEGGNVYATGGNNEGQLGLGDTEERNTFHVISFFTSEH
  KIKQLSAGSNTSAALTEDGRLFMWGDNSEGQIGLKNVSNVCVPQQVTIGKPVSWISCGYYHSAFVTTDGE
  LYVFGEPENGKLGLPNQLLGNHRTPQLVSEIPEKVIQVACGGEHTVVLTENAVYTFGLGQFGQLGLGTFL
  FETSEPKVIENIRDQTISYISCGENHTALITDIGLMYTFGDGRHGKLGLGLENFTNHFIPTLCSNFLRFI
  VKLVACGGCHMVVFAAPHRGVAKEIEFDEINDTCLSVATFLPYSSLTSGNVLQRTLSARMRRRERERSPD
  SFSMRRTLPPIEGTLGLSACFLPNSVFPRCSERNLQESVLSEQDLMQPEEPDYLLDEMTKEAEIDNSSTV
  ESLGETTDILNMTHIMSLNSNEKSLKLSPVQKQKKQQTIGELTQDTALTENDDSDEYEEMSEMKEGKACK
  QHVSQGIFMTQPATTIEAFSDEEVEIPEEKEGAEDSKGNGIEEQEVEANEENVKVHGGRKEKTEILSDDL
  TDKAEDHEFSKTEELKLEDVDEEINAENVESKKKTVGDDESVPTGYHSKTEGAERTNDDSSAETIEKKEK
  ANLEERAICEYNENPKGYMLDDADSSSLEILENSETTPSKDMKKTKKIFLFKRVPSINQKIVKNNNEPLP
  EIKSIGDQIILKSDNKDADQNHMSQNHQNIPPTNTERRSKSCTIL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000328
• ORF Size: 2445 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000328.3
• RefSeq Size: 3082 bp
• RefSeq ORF: 2448 bp
• Locus ID: 6103
• UniProt ID: Q92834
• Cytogenetics: Xp11.4
• Domains: RCC1
• Protein Families: Druggable Genome
• MW: 90.1 kDa
• Gene Summary: This gene encodes a protein with a series of six RCC1-like domains (RLDs), characteristic of the highly conserved guanine nucleotide exchange factors. The encoded protein is found in the Golgi body and interacts with RPGRIP1. This protein localizes to the outer segment of rod photoreceptors and is essential for their viability. Mutations in this gene have been associated with X-linked retinitis pigmentosa (XLRP). Multiple alternatively spliced transcript variants that encode different isoforms of this gene have been reported, but the full-length natures of only some have been determined. [provided by RefSeq, Dec 2008]