LIM kinase 2 (LIMK2) (NM_016733) Human Tagged ORF Clone

CAT#: RC220400

LIMK2 (Myc-DDK-tagged)-Human LIM domain kinase 2 (LIMK2), transcript variant 2b

Product Images

Circular map for RC220400

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  Western blot validation of overexpression lysate (Cat# LY413829) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220400 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified LIMK2 protein (Cat# TP320400). The protein was produced from HEK293T cells transfected with LIMK2 cDNA clone (Cat# RC220400) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LIM kinase 2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220400 representing NM_016733
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAGTTACTTGTCAGTCCCGGCTTACTTCACCTCCAGAGACCTGTTTCGGTGTTCAGAATGCCAGG
  ATTCCCTCACCAACTGGTACTATGAGAAGGATGGGAAGCTCTACTGCCCCAAGGACTACTGGGGGAAGTT
  TGGGGAGTTCTGTCATGGGTGCTCCCTGCTGATGACAGGGCCTTTTATGGTGGCTGGGGAGTTCAAGTAC
  CACCCAGAGTGCTTTGCCTGTATGAGCTGCAAGGTGATCATTGAGGATGGGGATGCATATGCACTGGTGC
  AGCATGCCACCCTCTACTGTGGGAAGTGCCACAATGAGGTGGTGCTGGCACCCATGTTTGAGAGACTCTC
  CACAGAGTCTGTTCAGGAGCAGCTGCCCTACTCTGTCACGCTCATCTCCATGCCGGCCACCACTGAAGGC
  AGGCGGGGCTTCTCCGTGTCCGTGGAGAGTGCCTGCTCCAACTACGCCACCACTGTGCAAGTGAAAGAGG
  TCAACCGGATGCACATCAGTCCCAACAATCGAAACGCCATCCACCCTGGGGACCGCATCCTGGAGATCAA
  TGGGACCCCCGTCCGCACACTTCGAGTGGAGGAGGTGGAGGATGCAATTAGCCAGACGAGCCAGACACTT
  CAGCTGTTGATTGAACATGACCCCGTCTCCCAACGCCTGGACCAGCTGCGGCTGGAGGCCCGGCTCGCTC
  CTCACATGCAGAATGCCGGACACCCCCACGCCCTCAGCACCCTGGACACCAAGGAGAATCTGGAGGGGAC
  ACTGAGGAGACGTTCCCTAAGGCGCAGTAACAGTATCTCCAAGTCCCCTGGCCCCAGCTCCCCAAAGGAG
  CCCCTGCTGTTCAGCCGTGACATCAGCCGCTCAGAATCCCTTCGTTGTTCCAGCAGCTATTCACAGCAGA
  TCTTCCGGCCCTGTGACCTAATCCATGGGGAGGTCCTGGGGAAGGGCTTCTTTGGGCAGGCTATCAAGGT
  GACACACAAAGCCACGGGCAAAGTGATGGTCATGAAAGAGTTAATTCGATGTGATGAGGAGACCCAGAAA
  ACTTTTCTGACTGAGGTGAAAGTGATGCGCAGCCTGGACCACCCCAATGTGCTCAAGTTCATTGGTGTGC
  TGTACAAGGATAAGAAGCTGAACCTGCTGACAGAGTACATTGAGGGGGGCACACTGAAGGACTTTCTGCG
  CAGTATGGATCCGTTCCCCTGGCAGCAGAAGGTCAGGTTTGCCAAAGGAATCGCCTCCGGAATGGCCTAT
  TTGCACTCTATGTGCATCATCCACCGGGATCTGAACTCGCACAACTGCCTCATCAAGTTGGACAAGACTG
  TGGTGGTGGCAGACTTTGGGCTGTCACGGCTCATAGTGGAAGAGAGGAAAAGGGCCCCCATGGAGAAGGC
  CACCACCAAGAAACGCACCTTGCGCAAGAACGACCGCAAGAAGCGCTACACGGTGGTGGGAAACCCCTAC
  TGGATGGCCCCTGAGATGCTGAACGGAAAGAGCTATGATGAGACGGTGGATATCTTCTCCTTTGGGATCG
  TTCTCTGTGAGATCATTGGGCAGGTGTATGCAGATCCTGACTGCCTTCCCCGAACACTGGACTTTGGCCT
  CAACGTGAAGCTTTTCTGGGAGAAGTTTGTTCCCACAGATTGTCCCCCGGCCTTCTTCCCGCTGGCCGCC
  ATCTGCTGCAGACTGGAGCCTGAGAGCAGACCAGCATTCTCGAAATTGGAGGACTCCTTTGAGGCCCTCT
  CCCTGTACCTGGGGGAGCTGGGCATCCCGCTGCCTGCAGAGCTGGAGGAGTTGGACCACACTGTGAGCAT
  GCAGTACGGCCTGACCCGGGACTCACCTCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220400 representing NM_016733
  Red=Cloning site Green=Tags(s)
  
  MGSYLSVPAYFTSRDLFRCSECQDSLTNWYYEKDGKLYCPKDYWGKFGEFCHGCSLLMTGPFMVAGEFKY
  HPECFACMSCKVIIEDGDAYALVQHATLYCGKCHNEVVLAPMFERLSTESVQEQLPYSVTLISMPATTEG
  RRGFSVSVESACSNYATTVQVKEVNRMHISPNNRNAIHPGDRILEINGTPVRTLRVEEVEDAISQTSQTL
  QLLIEHDPVSQRLDQLRLEARLAPHMQNAGHPHALSTLDTKENLEGTLRRRSLRRSNSISKSPGPSSPKE
  PLLFSRDISRSESLRCSSSYSQQIFRPCDLIHGEVLGKGFFGQAIKVTHKATGKVMVMKELIRCDEETQK
  TFLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTLKDFLRSMDPFPWQQKVRFAKGIASGMAY
  LHSMCIIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEERKRAPMEKATTKKRTLRKNDRKKRYTVVGNPY
  WMAPEMLNGKSYDETVDIFSFGIVLCEIIGQVYADPDCLPRTLDFGLNVKLFWEKFVPTDCPPAFFPLAA
  ICCRLEPESRPAFSKLEDSFEALSLYLGELGIPLPAELEELDHTVSMQYGLTRDSPP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016733
• ORF Size: 1851 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016733.1
• RefSeq Size: 3848 bp
• RefSeq ORF: 1854 bp
• Locus ID: 3985
• UniProt ID: P53671
• Cytogenetics: 22q12.2
• Domains: pkinase, TyrKc, PDZ, LIM, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton
• MW: 69.7 kDa
• Gene Summary: There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]