ASXL2 (NM_018263) Human Tagged ORF Clone

CAT#: RC220364

ASXL2 (Myc-DDK-tagged)-Human additional sex combs like 2 (Drosophila) (ASXL2)

Product Images

Circular map for RC220364

• 

  Western blot validation of overexpression lysate (Cat# LY413175) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220364 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ASXL2
• Synonyms: ASXH2; SHAPNS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220364 representing NM_018263
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGAAAAGGGACGTAGGAAGAAGGGCAGGACCTGGGCGGAGGCCGCCAAGACGGTCTTAGAAAAAT
  ACCCCAATACACCCATGAGTCATAAAGAAATTCTTCAAGTTATCCAGAGAGAAGGACTAAAAGAAATCAG
  AAGTGGGACTTCTCCTCTTGCATGCCTGAATGCAATGCTTCACACAAACTCCAGAGGTGAAGAGGGCATC
  TTCTATAAGGTTCCAGGTAGAATGGGAGTATATACTTTGAAGAAAGATGTGCCGGATGGGGTGAAAGAGC
  TGTCAGAAGGTTCAGAAGAAAGCAGTGATGGTCAGTCAGATTCCCAGAGTTCTGAGAACAGCAGCAGCAG
  CAGTGATGGTGGCAGCAACAAGGAGGGAAAAAAGAGCAGGTGGAAAAGGAAAGTATCGTCGTCCTCCCCG
  CAGTCAGGCTGCCCATCACCCACCATTCCAGCAGGTAAAGTCATTTCTCCATCACAGAAGCACAGCAAGA
  AGGCACTAAAGCAGGCGCTAAAGCAGCAACAGCAGAAGAAGCAGCAGCAGCAATGCAGGCCAAGCATATC
  CATCTCCTCCAACCAGCATCTCTCACTAAAGACTGTCAAAGCAGCCAGTGACTCTGTACCTGCCAAACCT
  GCAACATGGGAAGGAAAGCAATCTGATGGACAGACAGGCAGCCCTCAAAACTCAAACTCCAGCTTTTCTT
  CCTCAGTTAAAGTGGAAAATACTTTACTAGGCTTGGGGAAGAAGTCATTCCAGAGATCTGAGAGACTCCA
  TACCAGACAAATGAAAAGAACTAAATGTGCTGACATTGACGTTGAGACACCGGACTCCATTCTGGTTAAT
  ACAAATCTGCGAGCACTGATCAACAAGCACACATTTTCAGTCCTTCCTGGAGATTGCCAGCAACGACTGC
  TTTTACTACTCCCAGAGGTAGATCGACAGGTTGGTCCAGATGGTTTAATGAAGTTAAATGGCTCAGCCCT
  TAACAATGAATTCTTCACTTCAGCAGCCCAAGGCTGGAAGGAAAGACTCTCAGAAGGTGAGTTTACACCT
  GAGATGCAGGTGAGAATTCGACAAGAGATTGAGAAGGAGAAAAAAGTGGAGCCATGGAAAGAACAATTCT
  TTGAAAGCTACTATGGGCAGAGTTCTGGCCTGAGCCTTGAAGATTCTAAGAAATTGACAGCTTCTCCCAG
  TGATCCCAAAGTAAAGAAAACCCCAGCTGAACAACCAAAATCCATGCCTGTGTCAGAGGCCTCTCTTATC
  AGAATAGTTCCAGTAGTCTCCCAGTCAGAGTGTAAAGAAGAAGCATTGCAAATGTCATCACCAGGCAGAA
  AAGAAGAGTGTGAAAGCCAAGGTGAAGTGCAGCCGAACTTCTCCACATCTTCAGAGCCCCTGCTTTCCTC
  AGCTCTCAATACACATGAGCTTAGCAGCATTCTTCCCATCAAGTGCCCAAAGGATGAGGATCTCTTGGAG
  CAGAAGCCAGTCACCTCTGCTGAACAGGAATCTGAGAAGAACCATCTCACCACAGCTTCTAATTATAACA
  AAAGTGAAAGCCAAGAATCTTTAGTTACATCGCCAAGCAAACCCAAGAGTCCTGGGGTTGAAAAACCAAT
  AGTGAAGCCCACAGCAGGAGCGGGTCCACAGGAGACTAATATGAAAGAACCTCTAGCAACTCTTGTTGAT
  CAGAGCCCAGAAAGCCTCAAGAGGAAGTCTTCCCTCACCCAAGAAGAGGCCCCTGTGAGCTGGGAGAAGA
  GGCCACGTGTCACTGAGAATCGCCAGCACCAGCAGCCATTTCAGGTCTCACCACAGCCCTTTCTCAATAG
  AGGGGACAGAATCCAGGTGCGAAAAGTACCACCTCTCAAGATCCCGGTCTCCAGAATCTCCCCCATGCCG
  TTTCATCCATCGCAGGTCTCTCCCAGGGCTCGTTTTCCAGTCTCCATCACTAGTCCTAACAGAACAGGAG
  CCAGAACTCTTGCAGACATCAAAGCAAAAGCCCAACTGGTCAAAGCACAGAGGGCAGCAGCTGCCGCTGC
  CGCCGCAGCTGCTGCAGCCGCCTCAGTTGGAGGGACCATTCCAGGACCTGGCCCAGGGGGTGGACAAGGT
  CCAGGAGAGGGTGGTGAAGGGCAGACTGCTAGAGGAGGCAGTCCAGGCTCAGACAGAGTCAGTGAAACTG
  GAAAGGGCCCCACACTGGAACTGGCAGGAACTGGAAGCAGGGGAGGTACGAGAGAGCTTTTACCCTGTGG
  TCCAGAGACTCAGCCCCAGTCTGAGACCAAGACCACCCCAAGCCAGGCACAGCCTCATAGTGTCTCTGGA
  GCACAACTACAGCAAACCCCCCCAGTGCCTCCAACACCTGCCGTCAGTGGAGCATGCACAAGTGTCCCAT
  CACCAGCCCACATAGAGAAATTGGATAATGAAAAACTGAACCCCACCAGAGCAACAGCCACAGTGGCCTC
  TGTCAGCCATCCACAAGGGCCCAGTAGTTGCAGACAGGAGAAAGCACCTTCTCCAACAGGTCCTGCTCTA
  ATCTCAGGTGCCTCACCTGTTCATTGTGCAGCTGATGGCACAGTTGAGCTCAAAGCAGGTCCTAGTAAGA
  ATATACCTAACCCTTCAGCCTCATCAAAGACAGATGCTAGTGTGCCAGTGGCTGTAACTCCCTCCCCTTT
  AACATCTTTATTGACCACAGCCACTTTAGAAAAGCTTCCTGTACCCCAGGTCAGTGCAACTACAGCACCT
  GCTGGATCAGCTCCACCCTCGAGCACTTTGCCAGCAGCTTCTAGCCTTAAAACCCCAGGAACTTCTTTAA
  ACATGAATGGACCCACTTTAAGACCAACCTCTAGTATCCCTGCTAATAATCCTTTAGTGACTCAGCTGCT
  TCAAGGCAAAGATGTTCCCATGGAGCAAATTCTGCCTAAACCTCTCACCAAAGTTGAAATGAAAACGGTT
  CCACTGACTGCAAAAGAGGAAAGGGGGATGGGAGCGCTCATAGCTACCAACACAACAGAAAATAGCACCA
  GAGAGGAAGTTAATGAGAGACAGTCCCATCCAGCTACGCAGCAGCAGCTGGGCAAAACCTTGCAAAGTAA
  GCAGCTCCCCCAGGTTCCAAGGCCCCTTCAGCTCTTTTCAGCTAAGGAGCTGAGGGACTCCAGCATTGAC
  ACACACCAATACCACGAAGGACTAAGTAAAGCAACCCAAGATCAGATCCTTCAGACTCTCATTCAGAGGG
  TTCGGAGGCAGAATCTTCTCTCAGTTGTGCCGCCCTCACAGTTCAACTTCGCTCACTCAGGTTTCCAGCT
  GGAAGACATCTCCACAAGCCAGAGGTTCATGCTGGGTTTTGCTGGCAGAAGGACATCCAAACCTGCAATG
  GCAGGGCACTACTTACTGAATATTTCTACCTACGGCCGGGGCTCAGAGAGCTTTAGGAGGACCCATTCTG
  TAAACCCTGAAGATCGTTTTTGTCTAAGCAGCCCCACTGAAGCCTTGAAAATGGGATATACAGACTGTAA
  AAATGCAACAGGAGAGAGTAGCAGCAGCAAAGAAGATGACACTGATGAGGAAAGTACTGGTGATGAGCAG
  GAATCTGTCACAGTGAAAGAGGAGCCCCAGGTTTCCCAGAGTGCTGGCAAGGGTGACACAAGTTCAGGAC
  CTCACAGCAGGGAAACTCTATCTACCAGTGATTGCTTAGCTAGCAAGAATGTGAAGGCTGAGATACCATT
  GAATGAGCAAACCACTTTAAGTAAGGAGAATTACCTGTTCACTAGAGGCCAAACATTTGATGAAAAGACC
  CTAGCCAGAGATTTAATTCAGGCAGCACAGAAGCAGATGGCTCATGCAGTGAGAGGTAAGGCAATCCGTA
  GCAGCCCCGAGCTTTTCAGTTCTACTGTTCTTCCTCTGCCTGCAGACAGCCCCACCCACCAGCCCCTACT
  CCTTCCACCCCTGCAAACCCCGAAGTTGTATGGAAGCCCCACCCAGATAGGGCCAAGCTATAGAGGCATG
  ATCAATGTCTCCACCTCATCTGACATGGACCATAACTCTGCTGTACCAGGTAGCCAGGTATCTAGCAATG
  TAGGTGATGTCATGTCATTTTCAGTGACTGTCACTACCATCCCTGCTAGCCAAGCTATGAATCCCAGCAG
  CCATGGCCAGACCATTCCTGTTCAGGCGTTCTCCGAAGAGAACAGCATAGAGGGCACGCCTTCGAAATGT
  TACTGCCGCTTGAAAGCCATGATCATGTGCAAAGGCTGTGGCGCTTTCTGCCATGATGATTGCATCGGCC
  CCTCCAAACTGTGCGTCTCCTGCCTTGTCGTTCGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220364 representing NM_018263
  Red=Cloning site Green=Tags(s)
  
  MREKGRRKKGRTWAEAAKTVLEKYPNTPMSHKEILQVIQREGLKEIRSGTSPLACLNAMLHTNSRGEEGI
  FYKVPGRMGVYTLKKDVPDGVKELSEGSEESSDGQSDSQSSENSSSSSDGGSNKEGKKSRWKRKVSSSSP
  QSGCPSPTIPAGKVISPSQKHSKKALKQALKQQQQKKQQQQCRPSISISSNQHLSLKTVKAASDSVPAKP
  ATWEGKQSDGQTGSPQNSNSSFSSSVKVENTLLGLGKKSFQRSERLHTRQMKRTKCADIDVETPDSILVN
  TNLRALINKHTFSVLPGDCQQRLLLLLPEVDRQVGPDGLMKLNGSALNNEFFTSAAQGWKERLSEGEFTP
  EMQVRIRQEIEKEKKVEPWKEQFFESYYGQSSGLSLEDSKKLTASPSDPKVKKTPAEQPKSMPVSEASLI
  RIVPVVSQSECKEEALQMSSPGRKEECESQGEVQPNFSTSSEPLLSSALNTHELSSILPIKCPKDEDLLE
  QKPVTSAEQESEKNHLTTASNYNKSESQESLVTSPSKPKSPGVEKPIVKPTAGAGPQETNMKEPLATLVD
  QSPESLKRKSSLTQEEAPVSWEKRPRVTENRQHQQPFQVSPQPFLNRGDRIQVRKVPPLKIPVSRISPMP
  FHPSQVSPRARFPVSITSPNRTGARTLADIKAKAQLVKAQRAAAAAAAAAAAAASVGGTIPGPGPGGGQG
  PGEGGEGQTARGGSPGSDRVSETGKGPTLELAGTGSRGGTRELLPCGPETQPQSETKTTPSQAQPHSVSG
  AQLQQTPPVPPTPAVSGACTSVPSPAHIEKLDNEKLNPTRATATVASVSHPQGPSSCRQEKAPSPTGPAL
  ISGASPVHCAADGTVELKAGPSKNIPNPSASSKTDASVPVAVTPSPLTSLLTTATLEKLPVPQVSATTAP
  AGSAPPSSTLPAASSLKTPGTSLNMNGPTLRPTSSIPANNPLVTQLLQGKDVPMEQILPKPLTKVEMKTV
  PLTAKEERGMGALIATNTTENSTREEVNERQSHPATQQQLGKTLQSKQLPQVPRPLQLFSAKELRDSSID
  THQYHEGLSKATQDQILQTLIQRVRRQNLLSVVPPSQFNFAHSGFQLEDISTSQRFMLGFAGRRTSKPAM
  AGHYLLNISTYGRGSESFRRTHSVNPEDRFCLSSPTEALKMGYTDCKNATGESSSSKEDDTDEESTGDEQ
  ESVTVKEEPQVSQSAGKGDTSSGPHSRETLSTSDCLASKNVKAEIPLNEQTTLSKENYLFTRGQTFDEKT
  LARDLIQAAQKQMAHAVRGKAIRSSPELFSSTVLPLPADSPTHQPLLLPPLQTPKLYGSPTQIGPSYRGM
  INVSTSSDMDHNSAVPGSQVSSNVGDVMSFSVTVTTIPASQAMNPSSHGQTIPVQAFSEENSIEGTPSKC
  YCRLKAMIMCKGCGAFCHDDCIGPSKLCVSCLVVR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018263
• ORF Size: 4305 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018263.6
• RefSeq Size: 7231 bp
• RefSeq ORF: 4308 bp
• Locus ID: 55252
• UniProt ID: Q76L83
• Cytogenetics: 2p23.3
• MW: 153.6 kDa
• Gene Summary: This gene encodes a member of a family of epigenetic regulators that bind various histone-modifying enzymes and are involved in the assembly of transcription factors at specific genomic loci. Naturally occurring mutations in this gene are associated with cancer in several tissue types (breast, bladder, pancreas, ovary, prostate, and blood). This gene plays an important role in neurodevelopment, cardiac function, adipogenesis, and osteoclastogenesis. [provided by RefSeq, Feb 2017]