HECTD3 (NM_024602) Human Tagged ORF Clone

CAT#: RC220357

HECTD3 (Myc-DDK-tagged)-Human HECT domain containing 3 (HECTD3)

Product Images

Circular map for RC220357

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  Western blot validation of overexpression lysate (Cat# LY411194) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220357 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified HECTD3 protein (Cat# TP320357). The protein was produced from HEK293T cells transfected with HECTD3 cDNA clone (Cat# RC220357) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HECTD3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220357 representing NM_024602
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGTCCTGGCCCGGGCGCGGTGCTGGAGTCCCCCCGGCAGCTGCTGGGCCGCGTGCGCTTCTTGG
  CAGAGGCAGCGCGGAGCCTCCGCGCCGGGCGGCCGCTGCCAGCAGCGCTGGCTTTCGTGCCGCGAGAGGT
  GCTCTACAAGCTTTACAAGGACCCAGCGGGACCGTCGCGCGTGCTTCTGCCGGTGTGGGAGGCAGAGGGC
  CTGGGGCTGCGTGTGGGCGCCGCAGGCCCAGCCCCCGGTACCGGCTCCGGGCCCCTCCGCGCCGCCCGCG
  ACAGCATTGAGCTCCGGCGCGGCGCCTGCGTGCGCACCACGGGCGAGGAGCTGTGCAATGGCCACGGGCT
  CTGGGTGAAGCTGACAAAGGAGCAGCTGGCAGAGCACCTGGGCGACTGCGGGCTGCAGGAAGGCTGGCTG
  CTGGTGTGCCGCCCGGCGGAGGGCGGAGCCCGCCTGGTACCCATCGACACTCCCAACCACCTCCAGCGGC
  AGCAGCAGCTCTTTGGCGTGGATTATCGGCCGGTGCTCAGGTGGGAACAGGTGGTGGACCTGACATACTC
  ACATCGCCTGGGATCGAGACCTCAGCCGGCAGAGGCATACGCAGAAGCTGTACAAAGGCTACTCTATGTA
  CCCCCGACATGGACCTACGAGTGCGACGAGGACCTGATCCACTTCTTGTATGACCACCTGGGCAAGGAGG
  ATGAGAACCTGGGTAGCGTGAAGCAGTATGTGGAGAGCATAGACGTTTCCTCCTACACGGAGGAGTTCAA
  CGTGTCCTGCCTGACAGACAGCAATGCCGATACCTACTGGGAGAGCGATGGGTCCCAGTGCCAACACTGG
  GTACGGCTTACTATGAAGAAGGGCACCATTGTCAAGAAGCTGCTACTCACAGTGGATACCACAGATGACA
  ACTTTATGCCAAAGCGGGTGGTGGTCTATGGGGGTGAAGGGGACAACCTGAAGAAGCTGAGTGACGTGAG
  CATTGACGAGACCCTCATCGGGGATGTCTGTGTCCTGGAGGACATGACCGTCCACCTCCCGATCATCGAG
  ATCCGCATCGTGGAGTGCCGAGATGATGGGATTGATGTTCGTCTCCGAGGGGTCAAGATCAAGTCATCTA
  GACAGCGGGAACTAGGGTTGAATGCAGACCTGTTCCAGCCAACTAGTCTGGTGCGATATCCACGCCTAGA
  AGGCACCGACCCTGAAGTACTGTACCGCAGAGCTGTCCTCCTGCAGAGATTCATCAAGATCCTCGATAGT
  GTCCTGCACCACCTGGTACCTGCCTGGGACCACACACTGGGCACCTTCAGTGAGATTAAGCAAGTGAAGC
  AGTTCCTACTGCTGTCCCGCCAGCGGCCAGGCCTGGTGGCTCAGTGCCTGCGTGACTCTGAGAGCAGCAA
  GCCCAGCTTCATGCCACGCCTATACATCAACCGCCGTCTTGCCATGGAACACCGTGCCTGCCCCTCTCGA
  GACCCTGCCTGCAAGAATGCAGTCTTCACCCAGGTATATGAAGGCCTCAAGCCCTCTGACAAATATGAAA
  AGCCCCTGGACTACAGGTGGCCCATGCGCTATGACCAGTGGTGGGAGTGTAAATTTATTGCAGAAGGCAT
  CATTGACCAAGGGGGTGGTTTCCGGGACAGCCTGGCAGATATGTCAGAAGAGCTGTGCCCTAGCTCAGCG
  GATACCCCCGTGCCCCTGCCCTTCTTTGTACGCACAGCCAACCAGGGCAATGGCACTGGTGAGGCTCGGG
  ACATGTATGTACCCAACCCCTCCTGCCGAGACTTTGCCAAGTATGAATGGATCGGACAGCTGATGGGGGC
  TGCCCTTCGGGGTAAGGAGTTCCTGGTCCTGGCCCTGCCTGGTTTTGTGTGGAAGCAGCTTTCTGGTGAG
  GAGGTGAGCTGGAGCAAGGACTTCCCAGCTGTGGACTCTGTGCTGGTGAAGCTCCTGGAAGTGATGGAAG
  GAATGGACAAGGAGACGTTTGAGTTCAAGTTTGGGAAGGAACTAACATTCACCACTGTACTGAGTGACCA
  ACAGGTGGTGGAGCTGATCCCTGGGGGTGCAGGCATCGTCGTGGGATATGGGGACCGTTCTCGTTTCATC
  CAACTGGTCCAGAAGGCACGGCTAGAGGAGAGCAAGGAGCAGGTGGCAGCTATGCAGGCAGGTCTGCTGA
  AGGTGGTACCACAGGCTGTGCTGGACTTGCTGACCTGGCAAGAGTTGGAGAAGAAAGTGTGTGGGGATCC
  AGAGGTCACTGTGGATGCTCTGCGCAAGCTCACCCGGTTTGAGGACTTCGAGCCATCTGACTCGCGGGTG
  CAGTATTTCTGGGAGGCACTGAACAACTTCACCAACGAGGACCGGAGCCGCTTCCTGCGCTTTGTCACGG
  GCCGCAGTCGCCTGCCAGCACGGATCTACATCTACCCAGACAAGCTGGGCTACGAGACCACAGACGCGCT
  GCCCGAGTCTTCCACTTGCTCCAGCACCCTCTTCCTGCCACACTATGCCAGTGCCAAGGTATGCGAGGAG
  AAGCTCCGCTATGCGGCCTACAACTGCGTGGCCATCGACACTGACATGAGCCCTTGGGAGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220357 representing NM_024602
  Red=Cloning site Green=Tags(s)
  
  MAGPGPGAVLESPRQLLGRVRFLAEAARSLRAGRPLPAALAFVPREVLYKLYKDPAGPSRVLLPVWEAEG
  LGLRVGAAGPAPGTGSGPLRAARDSIELRRGACVRTTGEELCNGHGLWVKLTKEQLAEHLGDCGLQEGWL
  LVCRPAEGGARLVPIDTPNHLQRQQQLFGVDYRPVLRWEQVVDLTYSHRLGSRPQPAEAYAEAVQRLLYV
  PPTWTYECDEDLIHFLYDHLGKEDENLGSVKQYVESIDVSSYTEEFNVSCLTDSNADTYWESDGSQCQHW
  VRLTMKKGTIVKKLLLTVDTTDDNFMPKRVVVYGGEGDNLKKLSDVSIDETLIGDVCVLEDMTVHLPIIE
  IRIVECRDDGIDVRLRGVKIKSSRQRELGLNADLFQPTSLVRYPRLEGTDPEVLYRRAVLLQRFIKILDS
  VLHHLVPAWDHTLGTFSEIKQVKQFLLLSRQRPGLVAQCLRDSESSKPSFMPRLYINRRLAMEHRACPSR
  DPACKNAVFTQVYEGLKPSDKYEKPLDYRWPMRYDQWWECKFIAEGIIDQGGGFRDSLADMSEELCPSSA
  DTPVPLPFFVRTANQGNGTGEARDMYVPNPSCRDFAKYEWIGQLMGAALRGKEFLVLALPGFVWKQLSGE
  EVSWSKDFPAVDSVLVKLLEVMEGMDKETFEFKFGKELTFTTVLSDQQVVELIPGGAGIVVGYGDRSRFI
  QLVQKARLEESKEQVAAMQAGLLKVVPQAVLDLLTWQELEKKVCGDPEVTVDALRKLTRFEDFEPSDSRV
  QYFWEALNNFTNEDRSRFLRFVTGRSRLPARIYIYPDKLGYETTDALPESSTCSSTLFLPHYASAKVCEE
  KLRYAAYNCVAIDTDMSPWEE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024602
• ORF Size: 2583 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024602.6
• RefSeq Size: 3628 bp
• RefSeq ORF: 2586 bp
• Locus ID: 79654
• UniProt ID: Q5T447
• Cytogenetics: 1p34.1
• Domains: HECT
• Protein Families: Druggable Genome
• MW: 97.6 kDa
• Gene Summary: The protein encoded by this gene transfers ubiquitin from an E2 ubiquitin-conjugating enzyme to targeted substrates, leading to the degradation of those substrates. The encoded protein has been shown to transfer ubiquitin to TRIOBP to facilitate cell cycle progression, and to STX8. [provided by RefSeq, Dec 2012]