CPNE9 (NM_153635) Human Tagged ORF Clone

CAT#: RC219974

CPNE9 (Myc-DDK-tagged)-Human copine family member IX (CPNE9)

Product Images

Circular map for RC219974

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CPNE9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219974 representing NM_153635
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCTCGGCGGAGCCTCCGAGCGCAGCGTCCCGGCCACCAAGATTGAAATTACCGTGTCCTGCCGGA
  ACCTGCTAGACCTTGATACCTTCTCCAAGTCCGACCCCATGGTGGTGCTTTACACGCAGAGCCGGGCCAG
  CCAGGAGTGGCGGGAGTTCGGACGGACCGAGGTGATTGATAACACGCTGAACCCAGACTTCGTGCGCAAA
  TTCGTCCTCGACTATTTCTTTGAGGAAAAGCAAAATCTGCGCTTCGATGTGTACAACGTGGACTCCAAAA
  CCAACATCTCCAAACCGAAGGATTTCCTGGGACAAGCGTTCCTGGCCCTGGGAGAGGTGATTGGAGGCCA
  GGGCAGCCGAGTAGAGCGAACCCTCACGGGTGTACCAGGCAAGAAGTGTGGGACCATATTGCTGACTGCA
  GAAGAGCTTAGCAATTGTCGGGACATTGCCACCATGCAGCTGTGTGCAAACAAGCTGGACAAGAAGGACT
  TCTTTGGGAAATCAGACCCCTTCCTTGTGTTCTACAGGAGCAATGAGGATGGCACGTTCACCATCTGCCA
  CAAGACAGAGGTTGTGAAAAACACGCTGAATCCTGTGTGGCAGCCCTTCAGCATCCCTGTGCGGGCTCTG
  TGCAATGGAGACTATGACAGAACGGTGAAGATTGATGTGTACGACTGGGACCGGGATGGAAGCCACGATT
  TCATTGGTGAGTTCACCACCAGCTACCGGGAGCTGAGCAAGGCCCAGAACCAGTTCACAGTATATGAGGT
  TCTTAACCCTCGGAAGAAATGTAAGAAGAAGAAATATGTCAACTCAGGAACTGTGACGCTGCTCTCCTTC
  TCTGTGGACTCTGAATTCACTTTTGTTGATTACATCAAGGGAGGGACACAGCTGAACTTCACAGTAGCCA
  TTGACTTCACGGCTTCCAATGGGAATCCTCTGCAGCCTACCTCCCTGCACTACATGAGTCCCTACCAGCT
  CAGCGCCTATGCCATGGCCCTCAAGGCAGTGGGAGAGATCATCCAGGACTATGACAGTGATAAGCTCTTC
  CCAGCTTATGGCTTTGGGGCCAAGCTGCCCCCAGAGGGACGGATCTCCCACCAGTTCCCCCTGAACAACA
  ATGATGAGGACCCCAACTGTGCGGGCATCGAGGGTGTGCTGGAGAGCTATTTCCAGAGCCTGCGCACAGT
  GCAGCTCTATGGGCCCACCTACTTTGCTCCTGTCATCAACCAAGTGGCCAGGGCTGCAGCCAAGATCTCT
  GATGGCTCCCAGTACTATGTTCTGCTCATCATCACTGATGGGGTCATCTCTGACATGACGCAGACCAAGG
  AGGCCATCGTCAGCGCCTCCTCATTGCCCATGTCTATCATTATCGTCGGTGTAGGACCAGCCATGTTTGA
  GGCAATGGAAGAGTTGGACGGTGATGATGTGCGCGTGTCCTCTAGGGGACGCTACGCAGAGCGGGACATC
  GTTCAGTTCGTCCCATTCCGAGACTATGTTGACCGGTCGGGGAACCAGGTGTTGAGCATGGCCCGACTGG
  CCAAGGATGTGCTGGCCGAGATCCCGGAGCAGCTGCTGTCCTATATGCGCACCAGAGACATCCAGCCTCG
  GCCCCCACCCCCTGCCAACCCCAGCCCGATCCCAGCTCCAGAGCAGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219974 representing NM_153635
  Red=Cloning site Green=Tags(s)
  
  MSLGGASERSVPATKIEITVSCRNLLDLDTFSKSDPMVVLYTQSRASQEWREFGRTEVIDNTLNPDFVRK
  FVLDYFFEEKQNLRFDVYNVDSKTNISKPKDFLGQAFLALGEVIGGQGSRVERTLTGVPGKKCGTILLTA
  EELSNCRDIATMQLCANKLDKKDFFGKSDPFLVFYRSNEDGTFTICHKTEVVKNTLNPVWQPFSIPVRAL
  CNGDYDRTVKIDVYDWDRDGSHDFIGEFTTSYRELSKAQNQFTVYEVLNPRKKCKKKKYVNSGTVTLLSF
  SVDSEFTFVDYIKGGTQLNFTVAIDFTASNGNPLQPTSLHYMSPYQLSAYAMALKAVGEIIQDYDSDKLF
  PAYGFGAKLPPEGRISHQFPLNNNDEDPNCAGIEGVLESYFQSLRTVQLYGPTYFAPVINQVARAAAKIS
  DGSQYYVLLIITDGVISDMTQTKEAIVSASSLPMSIIIVGVGPAMFEAMEELDGDDVRVSSRGRYAERDI
  VQFVPFRDYVDRSGNQVLSMARLAKDVLAEIPEQLLSYMRTRDIQPRPPPPANPSPIPAPEQP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153635
• ORF Size: 1659 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153635.3
• RefSeq Size: 2066 bp
• RefSeq ORF: 1662 bp
• Locus ID: 151835
• UniProt ID: Q8IYJ1
• Cytogenetics: 3p25.3
• MW: 61.9 kDa
• Gene Summary: Probable calcium-dependent phospholipid-binding protein that may play a role in calcium-mediated intracellular processes (By similarity). Plays a role in dendrite formation by melanocytes (PubMed:23999003).[UniProtKB/Swiss-Prot Function]