Complement factor 8 beta (C8B) (NM_000066) Human Tagged ORF Clone

CAT#: RC219942

C8B (Myc-DDK-tagged)-Human complement component 8, beta polypeptide (C8B)

Product Images

Circular map for RC219942

• 

  Western blot validation of overexpression lysate (Cat# LY424942) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219942 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified C8B protein (Cat# TP319942). The protein was produced from HEK293T cells transfected with C8B cDNA clone (Cat# RC219942) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Complement factor 8 beta
• Synonyms: C82
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219942 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAATTCCAGGACATGGGCTTGGAGGGCGCCGGTGGAGCTATTTCTTCTCTGTGCTGCCCTGGGCT
  GTCTCAGTTTGCCTGGCTCCAGAGGTGAAAGGCCACATTCCTTTGGGTCAAATGCAGTCAACAAGAGCTT
  TGCTAAGAGCAGACAGATGCGGAGTGTGGATGTTACCCTGATGCCCATTGATTGTGAGCTGTCTAGTTGG
  TCCTCTTGGACCACATGTGACCCCTGTCAGAAGAAAAGGTACAGGTATGCCTACTTGCTCCAGCCCTCTC
  AGTTCCATGGGGAACCGTGCAACTTCTCTGACAAGGAAGTCGAAGACTGTGTTACCAACAGACCATGCAG
  AAGTCAAGTGCGATGTGAAGGCTTTGTGTGTGCACAGACAGGAAGGTGTGTAAACCGCAGACTTCTTTGC
  AATGGGGACAATGACTGTGGAGACCAGTCAGATGAAGCAAACTGTAGAAGGATTTATAAAAAATGTCAGC
  ATGAAATGGACCAATACTGGGGAATTGGCAGTCTGGCCAGTGGGATAAATTTGTTCACAAACAGTTTTGA
  GGGCCCAGTTCTTGATCACAGGTATTATGCAGGTGGATGCTCCCCGCATTACATCCTGAACACGAGGTTT
  AGGAAGCCCTACAATGTGGAAAGCTACACGCCACAGACCCAAGGCAAATACGAATTCATATTAAAAGAGT
  ATGAATCATACTCAGATTTTGAACGCAATGTCACAGAGAAAATGGCAAGCAAGTCTGGTTTCAGTTTTGG
  TTTTAAAATACCTGGAATATTTGAACTTGGCATCAGTAGTCAAAGTGATCGAGGCAAACACTATATTAGG
  AGAACCAAACGATTCTCTCATACTAAAAGCGTATTTCTGCATGCACGCTCTGACCTTGAAGTAGCACATT
  ACAAGCTGAAACCCAGAAGCCTCATGCTCCATTACGAGTTCCTTCAGAGAGTTAAGCGGCTGCCCCTGGA
  GTACAGCTACGGGGAATACAGAGATCTCTTCCGTGATTTTGGGACCCACTACATCACAGAGGCTGTGCTT
  GGGGGCATTTATGAATACACCCTCGTTATGAACAAAGAGGCCATGGAGAGAGGAGATTATACTCTTAACA
  ACGTCCATGCCTGTGCCAAAAATGATTTTAAAATTGGTGGTGCCATTGAAGAGGTCTACGTCAGTCTGGG
  TGTGTCTGTAGGCAAATGCAGAGGTATTCTGAATGAAATAAAAGACAGAAACAAGAGGGACACCATGGTG
  GAGGACTTGGTGGTCCTGGTACGAGGAGGGGCAAGTGAGCACATCACCACCCTGGCATACCAGGAGCTGC
  CGACGGCGGACCTGATGCAGGAGTGGGGAGACGCTGTGCAGTACAACCCAGCCATCATCAAAGTTAAGGT
  GGAGCCTCTGTATGAACTAGTGACAGCCACAGATTTTGCCTATTCCAGCACAGTGAGGCAGAACATGAAG
  CAGGCACTGGAGGAGTTCCAGAAGGAAGTTAGTTCCTGCCACTGTGCTCCCTGCCAAGGAAATGGAGTCC
  CTGTCCTGAAAGGATCACGCTGTGACTGCATCTGTCCTGTTGGATCCCAAGGCCTAGCCTGTGAGGTCTC
  CTATCGGAAGAATACCCCCATTGATGGGAAGTGGAATTGCTGGTCAAATTGGTCTTCATGCTCTGGAAGA
  CGTAAGACAAGACAAAGGCAGTGTAACAATCCACCTCCTCAAAATGGGGGTAGCCCCTGTTCAGGCCCTG
  CTTCAGAAACACTTGACTGCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219942 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKNSRTWAWRAPVELFLLCAALGCLSLPGSRGERPHSFGSNAVNKSFAKSRQMRSVDVTLMPIDCELSSW
  SSWTTCDPCQKKRYRYAYLLQPSQFHGEPCNFSDKEVEDCVTNRPCRSQVRCEGFVCAQTGRCVNRRLLC
  NGDNDCGDQSDEANCRRIYKKCQHEMDQYWGIGSLASGINLFTNSFEGPVLDHRYYAGGCSPHYILNTRF
  RKPYNVESYTPQTQGKYEFILKEYESYSDFERNVTEKMASKSGFSFGFKIPGIFELGISSQSDRGKHYIR
  RTKRFSHTKSVFLHARSDLEVAHYKLKPRSLMLHYEFLQRVKRLPLEYSYGEYRDLFRDFGTHYITEAVL
  GGIYEYTLVMNKEAMERGDYTLNNVHACAKNDFKIGGAIEEVYVSLGVSVGKCRGILNEIKDRNKRDTMV
  EDLVVLVRGGASEHITTLAYQELPTADLMQEWGDAVQYNPAIIKVKVEPLYELVTATDFAYSSTVRQNMK
  QALEEFQKEVSSCHCAPCQGNGVPVLKGSRCDCICPVGSQGLACEVSYRKNTPIDGKWNCWSNWSSCSGR
  RKTRQRQCNNPPPQNGGSPCSGPASETLDCS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000066
• ORF Size: 1773 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000066.3
• RefSeq Size: 2178 bp
• RefSeq ORF: 1776 bp
• Locus ID: 732
• UniProt ID: P07358
• Cytogenetics: 1p32.2
• Domains: tsp_1, MACPF, ldl_recept_a
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Complement and coagulation cascades, Prion diseases, Systemic lupus erythematosus
• MW: 67 kDa
• Gene Summary: This gene encodes one of the three subunits of the complement component 8 (C8) protein. C8 is composed of equimolar amounts of alpha, beta and gamma subunits, which are encoded by three separate genes. C8 is one component of the membrane attack complex, which mediates cell lysis, and it initiates membrane penetration of the complex. This protein mediates the interaction of C8 with the C5b-7 membrane attack complex precursor. In humans deficiency of this protein is associated with increased risk of meningococcal infections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]