EIF4G1 (NM_198241) Human Tagged ORF Clone

CAT#: RC219841

EIF4G1 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant 2

Product Images

Circular map for RC219841

• 

  Western blot validation of overexpression lysate (Cat# LY404962) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219841 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified EIF4G1 protein (Cat# TP319841). The protein was produced from HEK293T cells transfected with EIF4G1 cDNA clone (Cat# RC219841) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EIF4G1
• Synonyms: EIF-4G1; EIF4F; EIF4G; EIF4GI; P220; PARK18
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219841 representing NM_198241
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAAAGCTCCACAGTCCACAGGCCCCCCACCCGCCCCATCCCCCGGACTCCCACAGCCAGCGTTTC
  CCCCGGGGCAGACAGCGCCGGTGGTGTTCAGTACGCCACAAGCGACACAAATGAACACGCCTTCTCAGCC
  CCGCCAGCACTTCTACCCTAGCCGGGCCCAGCCCCCGAGCAGTGCAGCCTCCCGAGTGCAGAGTGCAGCC
  CCTGCCCGCCCTGGCCCAGCTGCCCATGTCTACCCTGCTGGATCCCAAGTAATGATGATCCCTTCCCAGA
  TCTCCTACCCAGCCTCCCAGGGGGCCTACTACATCCCTGGACAGGGGCGTTCCACATACGTTGTCCCGAC
  ACAGCAGTACCCTGTGCAGCCAGGAGCCCCAGGCTTCTATCCAGGTGCAAGCCCTACAGAATTTGGGACC
  TACGCTGGCGCCTACTATCCAGCCCAAGGGGTGCAGCAGTTTCCCACTGGCGTGGCCCCCGCCCCAGTTT
  TGATGAACCAGCCACCCCAGATTGCTCCCAAGAGGGAGCGTAAGACGATCCGAATTCGAGATCCAAACCA
  AGGAGGAAAGGATATCACAGAGGAGATCATGTCTGGGGCCCGCACTGCCTCCACACCCACCCCTCCCCAG
  ACGGGAGGCGGTCTGGAGCCTCAAGCTAATGGGGAGACGCCCCAGGTTGCTGTCATTGTCCGGCCAGATG
  ACCGGTCACAGGGAGCAATCATTGCTGACCGGCCAGGGCTGCCTGGCCCAGAGCATAGCCCTTCAGAATC
  CCAGCCTTCGTCGCCTTCTCCGACCCCATCACCATCCCCAGTCTTGGAACCGGGGTCTGAGCCTAATCTC
  GCAGTCCTCTCTATTCCTGGGGACACTATGACAACTATACAAATGTCTGTAGAAGAATCAACCCCCATCT
  CCCGTGAAACTGGGGAGCCATATCGCCTCTCTCCAGAACCCACTCCTCTCGCCGAACCCATACTGGAAGT
  AGAAGTGACACTTAGCAAACCGGTTCCAGAATCTGAGTTTTCTTCCAGTCCTCTCCAGGCTCCCACCCCT
  TTGGCATCTCACACAGTGGAAATTCATGAGCCTAATGGCATGGTCCCATCTGAAGATCTGGAACCAGAGG
  TGGAGTCAAGCCCAGAGCTTGCTCCTCCCCCAGCTTGCCCCTCCGAATCCCCTGTGCCCATTGCTCCAAC
  TGCCCAACCTGAGGAACTGCTCAACGGAGCCCCCTCGCCACCAGCTGTGGACTTAAGCCCAGTCAGTGAG
  CCAGAGGAGCAGGCCAAGGAGGTGACAGCATCAATGGCGCCCCCCACCATCCCCTCTGCTACTCCAGCTA
  CGGCTCCTTCAGCTACTTCCCCAGCTCAGGAGGAGGAAATGGAAGAAGAAGAAGAAGAGGAAGAAGGAGA
  AGCAGGAGAAGCAGGAGAAGCTGAGAGTGAGAAAGGAGGAGAGGAACTGCTCCCCCCAGAGAGTACCCCT
  ATTCCAGCCAACTTGTCTCAGAATTTGGAGGCAGCAGCAGCCACTCAAGTGGCAGTATCTGTGCCAAAGA
  GGAGACGGAAAATTAAGGAGCTAAATAAGAAGGAGGCTGTTGGAGACCTTCTGGATGCCTTCAAGGAGGC
  GAACCCGGCAGTACCAGAGGTGGAAAATCAGCCTCCTGCAGGCAGCAATCCAGGCCCAGAGTCTGAGGGC
  AGTGGTGTGCCCCCACGTCCTGAGGAAGCAGATGAGACCTGGGACTCAAAGGAAGACAAAATTCACAATG
  CTGAGAACATCCAGCCCGGGGAACAGAAGTATGAATATAAGTCAGATCAGTGGAAGCCTCTAAACCTAGA
  GGAGAAAAAACGTTACGACCGTGAGTTCCTGCTTGGTTTTCAGTTCATCTTTGCCAGTATGCAGAAGCCA
  GAGGGATTGCCACATATCAGTGACGTGGTGCTGGACAAGGCCAATAAAACACCACTGCGGCCACTGGATC
  CCACTAGACTACAAGGCATAAATTGTGGCCCAGACTTCACTCCATCCTTTGCCAACCTTGGCCGGACAAC
  CCTTAGCACCCGTGGGCCCCCAAGGGGTGGGCCAGGTGGGGAGCTGCCCCGTGGGCCGGCTGGCCTGGGA
  CCCCGGCGCTCTCAGCAGGGACCCCGAAAAGAACCACGCAAGATCATTGCCACAGTGTTAATGACCGAAG
  ATATAAAACTGAACAAAGCAGAGAAAGCCTGGAAACCCAGCAGCAAGCGGACGGCGGCTGATAAGGATCG
  AGGGGAAGAAGATGCTGATGGCAGCAAAACCCAGGACCTATTCCGCAGGGTGCGCTCCATCCTGAATAAA
  CTGACACCCCAGATGTTCCAGCAGCTGATGAAGCAAGTGACGCAGCTGGCCATCGACACCGAGGAACGCC
  TCAAAGGGGTCATTGACCTCATTTTTGAGAAGGCCATTTCAGAGCCCAACTTCTCTGTGGCCTATGCCAA
  CATGTGCCGCTGCCTCATGGCGCTGAAAGTGCCCACTACGGAAAAGCCAACAGTGACTGTGAACTTCCGA
  AAGCTGTTGTTGAATCGATGTCAGAAGGAGTTTGAGAAAGACAAAGATGATGATGAGGTTTTTGAGAAGA
  AGCAAAAAGAGATGGATGAAGCTGCTACGGCAGAGGAACGAGGACGCCTGAAGGAAGAGCTGGAAGAGGC
  TCGGGACATAGCCCGGCGGCGCTCTTTAGGGAATATCAAGTTTATTGGAGAGTTGTTCAAACTGAAGATG
  TTAACAGAGGCAATAATGCATGACTGTGTGGTCAAACTGCTTAAGAACCATGATGAAGAGTCCCTTGAGT
  GCCTTTGTCGTCTGCTCACCACCATTGGCAAAGACCTGGACTTTGAAAAAGCCAAGCCCCGAATGGATCA
  GTATTTCAACCAGATGGAAAAAATCATTAAAGAAAAGAAGACGTCATCCCGCATCCGCTTTATGCTGCAG
  GACGTGCTGGATCTGCGAGGGAGCAATTGGGTGCCACGCCGAGGGGATCAGGGTCCCAAGACCATTGACC
  AGATCCATAAGGAGGCTGAGATGGAAGAACATCGAGAGCACATCAAAGTGCAGCAGCTCATGGCCAAGGG
  CAGTGACAAGCGTCGGGGCGGTCCTCCAGGCCCTCCCATCAGCCGTGGACTTCCCCTTGTGGATGATGGT
  GGCTGGAACACAGTTCCCATCAGCAAAGGTAGCCGCCCCATTGACACCTCACGACTCACCAAGATCACCA
  AGCCTGGCTCCATCGATTCTAACAACCAGCTCTTTGCACCTGGAGGGCGACTGAGCTGGGGCAAGGGCAG
  CAGCGGAGGCTCAGGAGCCAAGCCCTCAGACGCAGCATCAGAAGCTGCTCGCCCAGCTACTAGTACTTTG
  AATCGCTTCTCAGCCCTTCAACAAGCGGTACCCACAGAAAGCACAGATAATAGACGTGTGGTGCAGAGGA
  GTAGCTTGAGCCGAGAACGAGGCGAGAAAGCTGGAGACCGAGGAGACCGCCTAGAGCGGAGTGAACGGGG
  AGGGGACCGTGGGGACCGGCTTGATCGTGCGCGGACACCTGCTACCAAGCGGAGCTTCAGCAAGGAAGTG
  GAGGAGCGGAGTAGAGAACGGCCCTCCCAGCCTGAGGGGCTGCGCAAGGCAGCTAGCCTCACGGAGGATC
  GGGACCGTGGGCGGGATGCCGTGAAGCGAGAAGCTGCCCTACCCCCAGTGAGCCCCCTGAAGGCGGCTCT
  CTCTGAGGAGGAGTTAGAGAAGAAATCCAAGGCTATCATTGAGGAATATCTCCATCTCAATGACATGAAA
  GAGGCAGTCCAGTGCGTGCAGGAGCTGGCCTCACCCTCCTTGCTCTTCATCTTTGTACGGCATGGTGTCG
  AGTCTACGCTGGAGCGCAGTGCCATTGCTCGTGAGCATATGGGGCAGCTGCTGCACCAGCTGCTCTGTGC
  TGGGCATCTGTCTACTGCTCAGTACTACCAAGGGTTGTATGAAATCTTGGAATTGGCTGAGGACATGGAA
  ATTGACATCCCCCACGTGTGGCTCTACCTAGCGGAACTGGTAACACCCATTCTGCAGGAAGGTGGGGTGC
  CCATGGGGGAGCTGTTCAGGGAGATTACAAAGCCTCTGAGACCGTTGGGCAAAGCTGCTTCCCTGTTGCT
  GGAGATCCTGGGCCTCCTGTGCAAAAGCATGGGTCCTAAAAAGGTGGGGACGCTGTGGCGAGAAGCCGGG
  CTTAGCTGGAAGGAATTTCTACCTGAAGGCCAGGACATTGGTGCATTCGTCGCTGAACAGAAGGTGGAGT
  ATACCCTGGGAGAGGAGTCGGAAGCCCCTGGCCAGAGGGCACTCCCCTCCGAGGAGCTGAACAGGCAGCT
  GGAGAAGCTGCTGAAGGAGGGCAGCAGTAACCAGCGGGTGTTCGACTGGATAGAGGCCAACCTGAGTGAG
  CAGCAGATAGTATCCAACACGTTAGTTCGAGCCCTCATGACGGCTGTCTGCTATTCTGCAATTATTTTTG
  AGACTCCCCTCCGAGTGGACGTTGCAGTGCTGAAAGCGCGAGCGAAGCTGCTGCAGAAATACCTGTGTGA
  CGAGCAGAAGGAGCTACAGGCGCTCTACGCCCTCCAGGCCCTTGTAGTGACCTTAGAACAGCCTCCCAAC
  CTGCTGCGGATGTTCTTTGACGCACTGTATGACGAGGACGTGGTGAAGGAGGATGCCTTCTACAGTTGGG
  AGAGTAGCAAGGACCCCGCTGAGCAGCAGGGCAAGGGTGTGGCCCTTAAATCTGTCACAGCCTTCTTCAA
  GTGGCTCCGTGAAGCAGAGGAGGAGTCTGACCACAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219841 representing NM_198241
  Red=Cloning site Green=Tags(s)
  
  MNKAPQSTGPPPAPSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQHFYPSRAQPPSSAASRVQSAA
  PARPGPAAHVYPAGSQVMMIPSQISYPASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGT
  YAGAYYPAQGVQQFPTGVAPAPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQ
  TGGGLEPQANGETPQVAVIVRPDDRSQGAIIADRPGLPGPEHSPSESQPSSPSPTPSPSPVLEPGSEPNL
  AVLSIPGDTMTTIQMSVEESTPISRETGEPYRLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQAPTP
  LASHTVEIHEPNGMVPSEDLEPEVESSPELAPPPACPSESPVPIAPTAQPEELLNGAPSPPAVDLSPVSE
  PEEQAKEVTASMAPPTIPSATPATAPSATSPAQEEEMEEEEEEEEGEAGEAGEAESEKGGEELLPPESTP
  IPANLSQNLEAAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPAVPEVENQPPAGSNPGPESEG
  SGVPPRPEEADETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKP
  EGLPHISDVVLDKANKTPLRPLDPTRLQGINCGPDFTPSFANLGRTTLSTRGPPRGGPGGELPRGPAGLG
  PRRSQQGPRKEPRKIIATVLMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNK
  LTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFR
  KLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKM
  LTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQ
  DVLDLRGSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDG
  GWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTL
  NRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEV
  EERSRERPSQPEGLRKAASLTEDRDRGRDAVKREAALPPVSPLKAALSEEELEKKSKAIIEEYLHLNDMK
  EAVQCVQELASPSLLFIFVRHGVESTLERSAIAREHMGQLLHQLLCAGHLSTAQYYQGLYEILELAEDME
  IDIPHVWLYLAELVTPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAG
  LSWKEFLPEGQDIGAFVAEQKVEYTLGEESEAPGQRALPSEELNRQLEKLLKEGSSNQRVFDWIEANLSE
  QQIVSNTLVRALMTAVCYSAIIFETPLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLEQPPN
  LLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFKWLREAEEESDHN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198241
• ORF Size: 4797 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198241.2, NP_937884.1
• RefSeq Size: 5463 bp
• RefSeq ORF: 4800 bp
• Locus ID: 1981
• UniProt ID: Q04637
• Cytogenetics: 3q27.1
• Protein Pathways: Viral myocarditis
• MW: 175.3 kDa
• Gene Summary: The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage. [provided by RefSeq, Aug 2010]