ZNF454 (NM_182594) Human Tagged ORF Clone

CAT#: RC219718

ZNF454 (Myc-DDK-tagged)-Human zinc finger protein 454 (ZNF454), transcript variant 2

Product Images

Circular map for RC219718

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ZNF454 (Cat# RC219718, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZNF45(Cat# TA811041). Positive lysates LY405474 (100ug) and LC405474 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY405474) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219718 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF454
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219718 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGTCAGCCACCTGCCAACCATGGTCCAGGAATCGGTGACCTTCAAGGATGTGGCTATACTGTTCA
  CCCAGGAAGAGTGGGGGCAGCTGAGCCCCGCCCAGAGGGCCCTGTACAGGGACGTGATGCTGGAGAACTA
  CAGCAACCTGGTCTCACTGGGACTCTTAGGACCCAAACCAGATACGTTTTCCCAGCTAGAAAAAAGGGAA
  GTGTGGATGCCAGAGGACACCCCTGGAGGCTTCTGTCTTGACTGGATGACTATGCCTGCCAGTAAGAAAT
  CTACTGTCAAGGCAGAGATTCCTGAAGAAGAATTGGATCAATGGACAATAAAGGAAAGATTCAGTAGCAG
  TAGTCACTGGAAGTGTGCTAGCCTGCTGGAGTGGCAATGTGGAGGCCAGGAGATCAGTTTGCAGCGAGTG
  GTACTCACTCACCCCAACACCCCATCACAGGAATGTGATGAATCCGGGAGCACTATGAGCTCATCTCTTC
  ACAGTGCTCAAAGTCAGGGATTTCAACCTAGCAAAAATGCCTTTGAGTGTAGTGAGTGTGGAAAAGTCTT
  CTCTAAGAGTTCAACTCTTAATAAACATCAGAAAATTCATAATGAAAAAAATGCAAATCAGAAAATTCAT
  ATTAAGGAGAAAAGATATGAATGTAGAGAATGTGGGAAAGCCTTTCACCAGAGTACGCACCTTATCCATC
  ACCAAAGAATTCACACTGGCGAGAAACCCTATGAATGTAAGGAATGTGGCAAGGCCTTCTCAGTGAGCTC
  CTCACTTACGTACCATCAGAAAATTCATACTGGAGAGAAGCCTTTTGAATGCAACTTATGTGGAAAAGCT
  TTTATCCGAAATATACACCTTGCCCATCATCATAGAATACATACTGGAGAGAAACCTTTTAAATGTAACA
  TTTGTGAAAAAGCCTTTGTGTGCAGGGCACACCTTACCAAACACCAGAATATCCACAGTGGAGAGAAACC
  CTATAAATGCAATGAATGTGGAAAAGCCTTTAATCAGAGTACAAGTTTCCTTCAGCATCAGAGAATTCAC
  ACTGGAGAGAAACCCTTTGAATGTAATGAATGTGGGAAGGCCTTCAGGGTGAACTCTTCCCTTACTGAAC
  ATCAGAGAATTCATACTGGAGAGAAACCTTATAAATGTAATGAATGTGGGAAAGCTTTCAGGGATAATTC
  ATCCTTTGCACGACATCGGAAAATTCACACTGGAGAGAAACCTTACAGATGTGGCTTGTGTGAGAAAGCC
  TTTCGGGACCAATCAGCACTAGCCCAACATCAGAGAATTCATACTGGGGAAAAACCTTATACATGTAACA
  TATGTGAAAAAGCCTTCAGTGACCATTCAGCCCTTACCCAACATAAGAGAATTCATACTAGGGAAAAACC
  TTACAAATGTAAAATCTGTGAGAAAGCCTTTATCCGAAGCACTCACCTGACTCAACATCAGAGGATTCAC
  ACAGGAGAGAAACCCTATAAATGTAATAAATGTGGGAAAGCTTTTAACCAGACTGCAAACCTCATTCAGC
  ATCAGAGACATCATATTGGAGAGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219718 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAVSHLPTMVQESVTFKDVAILFTQEEWGQLSPAQRALYRDVMLENYSNLVSLGLLGPKPDTFSQLEKRE
  VWMPEDTPGGFCLDWMTMPASKKSTVKAEIPEEELDQWTIKERFSSSSHWKCASLLEWQCGGQEISLQRV
  VLTHPNTPSQECDESGSTMSSSLHSAQSQGFQPSKNAFECSECGKVFSKSSTLNKHQKIHNEKNANQKIH
  IKEKRYECRECGKAFHQSTHLIHHQRIHTGEKPYECKECGKAFSVSSSLTYHQKIHTGEKPFECNLCGKA
  FIRNIHLAHHHRIHTGEKPFKCNICEKAFVCRAHLTKHQNIHSGEKPYKCNECGKAFNQSTSFLQHQRIH
  TGEKPFECNECGKAFRVNSSLTEHQRIHTGEKPYKCNECGKAFRDNSSFARHRKIHTGEKPYRCGLCEKA
  FRDQSALAQHQRIHTGEKPYTCNICEKAFSDHSALTQHKRIHTREKPYKCKICEKAFIRSTHLTQHQRIH
  TGEKPYKCNKCGKAFNQTANLIQHQRHHIGEK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182594
• ORF Size: 1566 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182594.4
• RefSeq Size: 2114 bp
• RefSeq ORF: 1569 bp
• Locus ID: 285676
• UniProt ID: Q8N9F8
• Cytogenetics: 5q35.3
• Protein Families: Transcription Factors
• MW: 60 kDa
• Gene Summary: May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]