CPNE8 (NM_153634) Human Tagged ORF Clone

CAT#: RC219637

CPNE8 (Myc-DDK-tagged)-Human copine VIII (CPNE8)

Product Images

Circular map for RC219637

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  Western blot validation of overexpression lysate (Cat# LY406995) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219637 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CPNE8 protein (Cat# TP319637). The protein was produced from HEK293T cells transfected with CPNE8 cDNA clone (Cat# RC219637) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CPNE8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219637 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAGCCGCTACAACAGCACTGCGGGCATCGGGGACTTGAACCAGCTGAGCGCTGCCATCCCGGCCA
  CGCGGGTGGAGGTGTCCGTGTCCTGCAGAAATCTTCTTGACAGAGACACATTTTCTAAATCTGATCCAAT
  TTGTGTCTTATATGTACAAGGAGTTGGAAATAAAGAATGGAGAGAGTTTGGAAGAACTGAAGTAATTGAT
  AATACTTTAAATCCTGATTTTGTAAGAAAGTTTATTCTGGACTACTTTTTTGAAGAAAGAGAGAATCTTC
  GTTTTGACTTGTATGATGTTGATTCAAAGAGCCCCAACTTATCCAAACATGACTTTCTGGGACAAGTGTT
  TTGTACATTGGGAGAGATCGTTGGTTCACAGGGAAGTCGCCTGGAAAAACCAATAGTAGGAATTCCAGGG
  AAGAAATGTGGTACAATCATACTTACAGCAGAGGAATTAAACTGTTGCAGGGATGCCGTTTTGATGCAAT
  TTTGTGCGAACAAATTGGACAAGAAGGACTTCTTTGGAAAATCAGATCCTTTCCTTGTATTTTATCGAAG
  TAATGAAGATGGCAGTTTTACAATTTGTCACAAGACAGAAGTTGTCAAAAACACTCTAAATCCAGTATGG
  CAAGCATTCAAGATCTCAGTCAGAGCATTATGTAATGGAGACTATGACAGAACAATCAAAGTAGAGGTGT
  ATGACTGGGACCGAGATGGAAGTCATGATTTCATTGGAGAATTTACAACAAGCTATAGGGAACTTTCTAG
  AGGGCAGTCACAATTCAACGTATATGAGGTGGTGAATCCCAAAAAGAAAGGAAAAAAGAAAAAATATACT
  AATTCTGGAACAGTAACTTTACTCTCTTTCTTGGTAGAAACAGAAGTTTCATTCCTTGACTACATTAAGG
  GAGGGACGCAAATCAATTTCACAGTGGCTATTGATTTTACAGCATCAAACGGCAACCCTGCTCAGCCCAC
  TTCCCTCCACTATATGAATCCTTACCAACTGAATGCCTATGGTATGGCACTAAAAGCAGTGGGAGAAATT
  GTTCAAGATTATGACAGTGATAAAATGTTTCCAGCTCTAGGATTTGGTGCAAAACTGCCTCCAGATGGAA
  GGATATCTCACGAATTTGCTTTGAATGGGAATCCTCAAAACCCCTACTGTGATGGCATTGAGGGGGTCAT
  GGAGGCTTATTACAGGAGTCTGAAATCTGTACAACTATATGGGCCCACCAACTTTGCTCCTGTAATTAAT
  CATGTAGCAAGATATGCTTCTTCTGTAAAGGATGGCTCCCAGTATTTTGTGCTTCTGATTGTTACAGATG
  GTGTTATCTCAGATATGGCCCAGACTAAGGAGTCCATAGTTAATGCCTCAAAACTTCCAATGTCAATAAT
  TATAGTAGGTGTTGGACCAGCAGAATTTGATGCAATGGTCGAATTGGATGGAGATGATGTAAGAGTCTCC
  TCTAGAGGAAAATATGCTGAAAGAGACATTGTGCAGTTTGTGCCATTCAGGGATTATATTGACAGAAGTG
  GAAACCACATACTGAGCATGGCTAGATTGGCTAAAGATGTCCTAGCTGAGATCCCTGAGCAGTTTCTCTC
  CTATATGAGAGCCCGAGGAATCAAGCCATCACCTGCGCCTCCCCCATACACCCCACCTACACATGTGTTA
  CAGACTCAAATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219637 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDSRYNSTAGIGDLNQLSAAIPATRVEVSVSCRNLLDRDTFSKSDPICVLYVQGVGNKEWREFGRTEVID
  NTLNPDFVRKFILDYFFEERENLRFDLYDVDSKSPNLSKHDFLGQVFCTLGEIVGSQGSRLEKPIVGIPG
  KKCGTIILTAEELNCCRDAVLMQFCANKLDKKDFFGKSDPFLVFYRSNEDGSFTICHKTEVVKNTLNPVW
  QAFKISVRALCNGDYDRTIKVEVYDWDRDGSHDFIGEFTTSYRELSRGQSQFNVYEVVNPKKKGKKKKYT
  NSGTVTLLSFLVETEVSFLDYIKGGTQINFTVAIDFTASNGNPAQPTSLHYMNPYQLNAYGMALKAVGEI
  VQDYDSDKMFPALGFGAKLPPDGRISHEFALNGNPQNPYCDGIEGVMEAYYRSLKSVQLYGPTNFAPVIN
  HVARYASSVKDGSQYFVLLIVTDGVISDMAQTKESIVNASKLPMSIIIVGVGPAEFDAMVELDGDDVRVS
  SRGKYAERDIVQFVPFRDYIDRSGNHILSMARLAKDVLAEIPEQFLSYMRARGIKPSPAPPPYTPPTHVL
  QTQI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153634
• ORF Size: 1692 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153634.3
• RefSeq Size: 3476 bp
• RefSeq ORF: 1695 bp
• Locus ID: 144402
• UniProt ID: Q86YQ8
• Cytogenetics: 12q12
• MW: 63.1 kDa
• Gene Summary: Calcium-dependent membrane-binding proteins may regulate molecular events at the interface of the cell membrane and cytoplasm. This gene is one of several genes that encode a calcium-dependent protein containing two N-terminal type II C2 domains and an integrin A domain-like sequence in the C-terminus. [provided by RefSeq, Jul 2008]