Argonaute 4 (AGO4) (NM_017629) Human Tagged ORF Clone

CAT#: RC219632

AGO4 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 2C, 4 (EIF2C4)

Product Images

Circular map for RC219632

• 

  Western blot validation of overexpression lysate (Cat# LY402604) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219632 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Argonaute 4
• Synonyms: EIF2C4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219632 representing NM_017629
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCGCTGGGACCCGGACCTCCGGCTAGCCTGTTTCAGCCACCTCGTCGTCCTGGCCTTGGAACTG
  TTGGAAAACCAATTCGACTGTTAGCCAATCATTTTCAGGTTCAGATTCCTAAAATAGATGTGTATCACTA
  TGATGTGGATATTAAGCCTGAAAAACGGCCTCGTAGAGTCAACAGGGAGGTAGTAGATACAATGGTGCGG
  CACTTCAAGATGCAAATATTTGGTGATCGGCAGCCTGGGTATGATGGCAAAAGAAACATGTACACAGCAC
  ATCCACTACCAATTGGACGGGATAGGGTTGATATGGAGGTGACTCTTCCAGGCGAGGGTAAAGACCAAAC
  ATTTAAAGTGTCTGTTCAGTGGGTGTCAGTTGTGAGCCTTCAGTTGCTTTTAGAAGCTTTGGCTGGGCAC
  TTGAATGAAGTCCCAGATGACTCAGTACAAGCACTTGATGTTATCACAAGACACCTTCCCTCCATGAGGT
  ACACCCCAGTGGGCCGTTCCTTTTTCTCACCCCCGGAAGGTTACTACCACCCTCTGGGAGGGGGCAGGGA
  GGTCTGGTTTGGTTTTCATCAGTCTGTGAGACCTGCCATGTGGAATATGATGCTCAACATTGATGTATCT
  GCAACTGCTTTCTACCGGGCTCAGCCTATCATTGAGTTCATGTGTGAGGTTTTAGACATTCAGAACATCA
  ATGAACAGACCAAACCTCTAACAGACTCCCAGCGTGTCAAATTTACCAAAGAAATCAGAGGTCTCAAAGT
  TGAGGTGACCCACTGTGGACAGATGAAACGAAAATACCGAGTTTGTAATGTGACTAGACGGCCAGCCAGT
  CATCAAACTTTTCCTTTGCAGCTAGAAAACGGTCAAGCTATGGAATGTACAGTAGCTCAATATTTTAAGC
  AAAAGTATAGTCTGCAACTGAAATACCCCCATCTTCCCTGTCTCCAAGTGGGACAAGAACAAAAGCATAC
  ATACTTGCCACTCGAGGTCTGTAATATAGTGGCAGGACAGCGATGTATCAAGAAGCTCACAGACAATCAG
  ACTTCCACAATGATCAAAGCTACAGCAAGATCTGCTCCTGACAGACAGGAAGAGATCAGTAGACTGGTGA
  AGAGCAACAGTATGGTGGGTGGACCTGATCCATACCTTAAAGAATTTGGTATTGTTGTCCACAATGAAAT
  GACAGAGCTCACAGGCAGGGTACTTCCAGCACCAATGCTGCAATATGGAGGCCGGAATAAAACAGTAGCC
  ACACCCAACCAGGGTGTCTGGGACATGCGAGGAAAGCAGTTTTATGCTGGCATTGAAATTAAAGTTTGGG
  CAGTTGCTTGTTTTGCACCTCAGAAACAATGTAGGGAAGATTTACTAAAGAGTTTCACTGACCAGCTGCG
  TAAAATCTCTAAGGATGCAGGAATGCCCATCCAGGGTCAGCCATGTTTCTGCAAGTATGCACAAGGTGCA
  GACAGTGTGGAGCCTATGTTTAAACATCTGAAAATGACTTATGTGGGCCTACAGCTAATAGTGGTTATCC
  TGCCTGGAAAGACACCAGTATATGCGGAGGTGAAACGTGTTGGAGATACCCTTCTAGGTATGGCCACACA
  GTGTGTCCAGGTAAAAAATGTAGTGAAGACCTCACCTCAAACCCTTTCCAATCTTTGCCTGAAGATAAAT
  GCAAAACTTGGAGGAATTAACAATGTGCTTGTGCCTCATCAAAGGCCCTCGGTGTTCCAGCAGCCTGTCA
  TCTTCCTGGGAGCGGATGTCACACACCCCCCAGCAGGGGATGGGAAGAAACCTTCCATTGCTGCTGTGGT
  TGGCAGTATGGATGGCCACCCCAGCCGGTACTGTGCCACCGTTCGGGTGCAGACTTCCCGGCAGGAGATC
  TCCCAAGAGCTCCTCTACAGTCAAGAGGTCATCCAGGACCTGACTAACATGGTTCGAGAGCTGCTGATTC
  AGTTCTACAAATCCACACGCTTCAAACCCACTCGGATCATCTATTACCGTGGAGGGGTATCTGAGGGACA
  AATGAAACAGGTAGCTTGGCCAGAACTAATAGCAATTCGAAAGGCATGTATTAGCTTGGAAGAAGATTAC
  CGGCCAGGAATAACTTATATTGTGGTGCAAAAAAGACATCACACACGACTCTTCTGTGCAGATAAAACAG
  AAAGGGTAGGGAAAAGTGGCAATGTACCAGCAGGCACTACAGTGGATAGTACCATCACACATCCATCTGA
  GTTTGACTTTTACCTCTGTAGTCATGCAGGAATTCAGGGAACCAGCCGTCCCTCACATTACCAGGTCTTG
  TGGGATGACAACTGCTTCACTGCAGATGAACTCCAGCTACTGACTTACCAGCTGTGTCACACCTATGTGA
  GGTGCACTCGCTCAGTCTCTATTCCAGCCCCTGCATATTATGCCCGGCTTGTAGCATTTAGGGCAAGGTA
  TCATCTGGTGGATAAAGATCATGACAGTGCGGAAGGCAGTCATGTGTCAGGACAGAGCAACGGCCGGGAT
  CCTCAGGCCTTGGCTAAGGCTGTGCAAATCCACCATGATACCCAGCACACGATGTATTTTGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219632 representing NM_017629
  Red=Cloning site Green=Tags(s)
  
  MEALGPGPPASLFQPPRRPGLGTVGKPIRLLANHFQVQIPKIDVYHYDVDIKPEKRPRRVNREVVDTMVR
  HFKMQIFGDRQPGYDGKRNMYTAHPLPIGRDRVDMEVTLPGEGKDQTFKVSVQWVSVVSLQLLLEALAGH
  LNEVPDDSVQALDVITRHLPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWNMMLNIDVS
  ATAFYRAQPIIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTRRPAS
  HQTFPLQLENGQAMECTVAQYFKQKYSLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQ
  TSTMIKATARSAPDRQEEISRLVKSNSMVGGPDPYLKEFGIVVHNEMTELTGRVLPAPMLQYGGRNKTVA
  TPNQGVWDMRGKQFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGA
  DSVEPMFKHLKMTYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKIN
  AKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQEI
  SQELLYSQEVIQDLTNMVRELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDY
  RPGITYIVVQKRHHTRLFCADKTERVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYQVL
  WDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRD
  PQALAKAVQIHHDTQHTMYFA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017629
• ORF Size: 2583 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017629.4
• RefSeq Size: 4758 bp
• RefSeq ORF: 2586 bp
• Locus ID: 192670
• UniProt ID: Q9HCK5
• Cytogenetics: 1p34.3
• Domains: Piwi
• Protein Families: Druggable Genome
• MW: 96.9 kDa
• Gene Summary: This gene encodes a member of the Argonaute family of proteins which contain PAZ and PIWI domains and play an integral role in RNA interference and short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a cluster of related family members. [provided by RefSeq, Mar 2017]