DGKH (NM_178009) Human Tagged ORF Clone

CAT#: RC219593

DGKH (Myc-DDK-tagged)-Human diacylglycerol kinase, eta (DGKH), transcript variant 2

Product Images

Circular map for RC219593

• 

  Western blot validation of overexpression lysate (Cat# LY406046) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219593 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified DGKH protein (Cat# TP319593). The protein was produced from HEK293T cells transfected with DGKH cDNA clone (Cat# RC219593) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DGKH
• Synonyms: DGKeta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219593 representing NM_178009
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGGGGCCGGAGGCCAGCACCACCCTCCGGGCGCCGCTGGAGGAGCGGCCGCCGGAGCCGGCGCCG
  CGGTCACCTCCGCCGCTGCCTCGGCGGGGCCGGGAGAGGATTCGTCTGACAGCGAAGCGGAGCAAGAGGG
  ACCCCAGAAACTGATCCGCAAAGTGTCTACCTCGGGGCAGATCCGGACCAAGACCAGTATTAAAGAGGGA
  CAGCTATTGAAGCAAACCAGTTCTTTCCAAAGGTGGAAAAAGCGATACTTCAAACTTCGAGGCCGCACCC
  TTTACTATGCAAAGGACTCAAAGTCTCTGATATTTGATGAAGTTGACCTCTCAGATGCTAGTGTAGCTGA
  AGCAAGCACGAAAAATGCTAACAACAGCTTCACGATCATCACTCCATTCAGAAGGCTAATGCTGTGTGCT
  GAGAACAGAAAGGAGATGGAGGATTGGATCAGCTCACTGAAGTCTGTACAGACCAGAGAACCCTACGAGG
  TGGCCCAGTTTAATGTGGAACATTTCTCAGGGATGCACAACTGGTACGCCTGCTCCCACGCCCGACCCAC
  CTTCTGTAACGTGTGCAGAGAGAGTCTTTCTGGAGTCACCTCCCATGGCCTGTCCTGCGAAGTGTGTAAA
  TTCAAGGCTCACAAAAGATGTGCAGTGAGAGCAACAAATAACTGTAAATGGACTACCCTGGCCTCCATCG
  GGAAGGACATTATAGAAGATGAAGATGGCGTCGCGATGCCTCACCAGTGGCTTGAGGGCAACCTGCCTGT
  AAGTGCCAAGTGTGCTGTCTGCGACAAAACATGTGGCAGTGTTCTCCGTCTACAGGATTGGAAATGCCTT
  TGGTGTAAGACAATGGTACACACTGCCTGCAAAGATTTATACCATCCAATATGTCCACTTGGTCAATGTA
  AAGTATCTATCATACCTCCAATTGCACTAAACAGCACCGATTCCGATGGTTTCTGTAGAGCAACATTTTC
  GTTCTGTGTTAGTCCTCTATTGGTTTTTGTCAATTCTAAGAGTGGAGATAATCAGGGAGTAAAGTTCCTC
  CGTCGCTTTAAACAGTTGCTAAATCCGGCTCAGGTGTTTGATTTAATGAATGGAGGTCCTCATTTAGGTT
  TAAGATTATTTCAGAAGTTTGACAATTTCCGGATTCTTGTTTGTGGAGGCGATGGAAGTGTAGGTTGGGT
  TTTGTCAGAAATCGATAAGCTCAACTTGAATAAACAGTGTCAGCTGGGAGTGTTGCCTTTGGGTACAGGA
  AATGACCTTGCCCGAGTTCTTGGCTGGGGAGGTTCATATGACGATGACACCCAACTTCCTCAGATCCTAG
  AGAAACTGGAACGAGCCAGTACCAAAATGTTGGACAGGTGGAGTATAATGACATATGAACTCAAATTGCC
  ACCAAAAGCTTCCCTACTTCCAGGACCTCCAGAAGCATCTGAAGAATTTTATATGACGATTTATGAAGAC
  TCAGTTGCAACGCATCTTACAAAAATCCTCAATTCTGATGAACATGCAGTGGTCATATCTTCTGCCAAGA
  CGCTATGTGAAACTGTAAAGGACTTCGTTGCCAAAGTAGAAAAGACGTATGACAAAACCTTGGAAAATGC
  CGTTGTAGCTGATGCCGTGGCCAGTAAATGTTCAGTCCTAAACGAGAAGCTCGAACAACTGCTGCAGGCT
  TTGCACACAGATTCCCAGGCTGCGCCTGTTCTCCCTGGCCTCAGCCCTCTCATTGTGGAAGAAGATGCTG
  TGGAATCGTCCAGTGAAGAGTCCCTGGGTGAAAGCAAAGAGCAGCTTGGGGATGACGTTACAAAACCTTC
  CTCCCAGAAAGCCGTCAAACCAAGGGAAATCATGTTGCGGGCAAATAGTTTAAAGAAAGCAGTGAGGCAA
  GTCATTGAGGAAGCCGGAAAAGTTATGGATGACCCGACAGTTCACCCCTGTGAACCAGCTAATCAGTCCT
  CTGATTATGACAGCACAGAAACAGATGAATCTAAGGAGGAAGCTAAAGATGATGGTGCCAAAGAATCAAT
  AACTGTTAAAACTGCACCTCGGTCTCCAGATGCCCGGGCAAGTTATGGCCATTCCCAAACTGATTCTGTC
  CCTGGTCCAGCTGTGGCAGCCAGCAAAGAAAACCTCCCTGTGCTCAATACCAGAATAATCTGCCCAGGTT
  TAAGAGCAGGACTGGCTGCCTCAATTGCTGGGAGTTCGATTATCAACAAAATGTTACTGGCAAACATTGA
  TCCTTTTGGTGCCACGCCGTTTATTGACCCGGATCTAGATTCCGTAGATGGATATTCAGAAAAATGTGTC
  ATGAACAATTACTTTGGGATTGGATTAGATGCAAAAATTTCATTAGAATTTAATAATAAAAGAGAGGAGC
  ACCCTGAAAAATGCAGGAGCCGAACTAAAAACTTGATGTGGTATGGAGTCCTTGGAACCCGGGAGTTATT
  ACAGAGATCGTACAAGAATTTAGAACAAAGGGTTCAACTTGAGTGTGATGGGCAGTATATTCCTCTTCCC
  AGCTTGCAAGGCATAGCCGTGTTGAACATTCCCAGCTATGCTGGAGGCACTAACTTTTGGGGTGGAACTA
  AAGAGGATGATATATTTGCTGCACCATCCTTTGATGACAAGATCCTGGAAGTTGTAGCAATATTTGATAG
  CATGCAAATGGCAGTTTCAAGGGTCATTAAACTGCAGCATCATCGAATAGCCCAGTGCCGTACAGTGAAA
  ATCACTATATTTGGTGACGAAGGAGTCCCAGTGCAAGTGGATGGTGAAGCGTGGGTTCAGCCTCCAGGGA
  TTATCAAAATTGTGCACAAAAACAGAGCACAAATGCTAACAAGGGACAGAGCCTTTGAGAGCACTCTGAA
  ATCTTGGGAAGATAAGCAGAAGTGTGATTCTGGTAAACCAGTTCTCCGAACCCATTTGTACATCCATCAC
  GCCATTGACTTGGCAACAGAAGAGGTGTCGCAGATGCAGCTATGCTCCCAGGCTGCAGAGGAGCTCATTA
  CTAGGATATGTGACGCAGCCACAATTCACTGTCTTTTGGAGCAAGAACTGGCCCATGCTGTGAATGCCTG
  CTCCCATGCCCTGAATAAAGCCAACCCAAGGTGCCCGGAGAGTCTTACAAGAGACACTGCCACTGAAATA
  GCCATCAATGTGAAGGCGCTGTATAATGAAACAGAATCTTTGCTAGTTGGCAGGGTTCCTTTGCAGCTGG
  AATCGCCACATGAAGAGCGAGTATCCAATGCCTTACACTCTGTGGAGGTGGAATTACAGAAACTGACAGA
  GATTCCTTGGCTTTATTATATCTTACACCCAAATGAGGATGAGGAACCTCCTATGGATTGCACCAAAAGG
  AACAACAGAAGCACCGTATTTCGAATAGTGCCAAAGTTTAAAAAGGAAAAGGTTCAGAAGCAGAAGACAA
  GTTCACAGCCTGTTCAGAAATGGGGCACAGAGGAAGTTGCTGCTTGGCTGGATCTGCTCAATTTGGGAGA
  GTACAAAGATATCTTCATCCGTCATGACATCAGAGGGGCTGAACTTTTGCATCTGGAAAGGCGAGATCTT
  AAGGATCTGGGGATACCGAAAGTGGGTCATGTGAAGCGAATTCTCCAGGGAATTAAAGAGCTTGGAAGGA
  GCACTCCACAGTCGGAGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219593 representing NM_178009
  Red=Cloning site Green=Tags(s)
  
  MAGAGGQHHPPGAAGGAAAGAGAAVTSAAASAGPGEDSSDSEAEQEGPQKLIRKVSTSGQIRTKTSIKEG
  QLLKQTSSFQRWKKRYFKLRGRTLYYAKDSKSLIFDEVDLSDASVAEASTKNANNSFTIITPFRRLMLCA
  ENRKEMEDWISSLKSVQTREPYEVAQFNVEHFSGMHNWYACSHARPTFCNVCRESLSGVTSHGLSCEVCK
  FKAHKRCAVRATNNCKWTTLASIGKDIIEDEDGVAMPHQWLEGNLPVSAKCAVCDKTCGSVLRLQDWKCL
  WCKTMVHTACKDLYHPICPLGQCKVSIIPPIALNSTDSDGFCRATFSFCVSPLLVFVNSKSGDNQGVKFL
  RRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDNFRILVCGGDGSVGWVLSEIDKLNLNKQCQLGVLPLGTG
  NDLARVLGWGGSYDDDTQLPQILEKLERASTKMLDRWSIMTYELKLPPKASLLPGPPEASEEFYMTIYED
  SVATHLTKILNSDEHAVVISSAKTLCETVKDFVAKVEKTYDKTLENAVVADAVASKCSVLNEKLEQLLQA
  LHTDSQAAPVLPGLSPLIVEEDAVESSSEESLGESKEQLGDDVTKPSSQKAVKPREIMLRANSLKKAVRQ
  VIEEAGKVMDDPTVHPCEPANQSSDYDSTETDESKEEAKDDGAKESITVKTAPRSPDARASYGHSQTDSV
  PGPAVAASKENLPVLNTRIICPGLRAGLAASIAGSSIINKMLLANIDPFGATPFIDPDLDSVDGYSEKCV
  MNNYFGIGLDAKISLEFNNKREEHPEKCRSRTKNLMWYGVLGTRELLQRSYKNLEQRVQLECDGQYIPLP
  SLQGIAVLNIPSYAGGTNFWGGTKEDDIFAAPSFDDKILEVVAIFDSMQMAVSRVIKLQHHRIAQCRTVK
  ITIFGDEGVPVQVDGEAWVQPPGIIKIVHKNRAQMLTRDRAFESTLKSWEDKQKCDSGKPVLRTHLYIHH
  AIDLATEEVSQMQLCSQAAEELITRICDAATIHCLLEQELAHAVNACSHALNKANPRCPESLTRDTATEI
  AINVKALYNETESLLVGRVPLQLESPHEERVSNALHSVEVELQKLTEIPWLYYILHPNEDEEPPMDCTKR
  NNRSTVFRIVPKFKKEKVQKQKTSSQPVQKWGTEEVAAWLDLLNLGEYKDIFIRHDIRGAELLHLERRDL
  KDLGIPKVGHVKRILQGIKELGRSTPQSEV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178009
• ORF Size: 3660 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178009.5
• RefSeq Size: 4248 bp
• RefSeq ORF: 3663 bp
• Locus ID: 160851
• UniProt ID: Q86XP1
• Cytogenetics: 13q14.11
• Protein Families: Druggable Genome
• Protein Pathways: Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• MW: 134.7 kDa
• Gene Summary: This gene encodes a member of the diacylglycerol kinase (DGK) enzyme family. Members of this family are involved in regulating intracellular concentrations of diacylglycerol and phosphatidic acid. Variation in this gene has been associated with bipolar disorder. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Jul 2014]