MOV10L1 (NM_018995) Human Tagged ORF Clone

CAT#: RC219511

MOV10L1 (Myc-DDK-tagged)-Human Mov10l1, Moloney leukemia virus 10-like 1, homolog (mouse) (MOV10L1), transcript variant 1

Product Images

Circular map for RC219511

• 

  Western blot validation of overexpression lysate (Cat# LY412833) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219511 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MOV10L1
• Synonyms: CHAMP; DJ402G11.8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219511 representing NM_018995
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAGCCTCGCAGCCAAGCTGGTGGCCTTCTTCTGGAGGACGGCGGACACCCCTAGGGAGGAAGCCG
  GGCAGCTGGAGCCCGAGCTCGCGGAAGGTGACACTAAGCTGAAAACTGTACGGGGTGTCGTGACAAGGTA
  CTGCAGCGATTATGGCATGATTGATGATATGATCTACTTCTCCAGTGATGCTGTGACTAGCAGAGTGCTT
  CTGAATGTTGGACAGGAAGTGATTGCAGTTGTGGAAGAAAATAAAGTGTCCAATGGACTGAAAGCAATCA
  GGGTAGAAGCTGTCTCTGATAAGTGGGAAGACGACAGCAGAAACCATGGGAGTCCCTCAGACTGCGGCCC
  CCGAGTGTTGATTGGCTGTGTGACTTCCCTGGTGGAGGGCGCAGGCTGTATCAGTCAGACCACCTACTTC
  TCTCTGGAGAGTGTGTGCGAAGGCTTCGAGCCCTGCAAGGGAGACTGGGTGGAGGCTGAGTACCGGATCC
  GGCCTGGCACGTGGAGCAGCGAAGCCACCTCAGTGAAGCCACTGAGATACAAGCGCGTGGACAAGGTCTG
  CATCTCTAGCCTCTGTGGAAGGAACGGGGTGTTAGAGGAAAGCATCTTCTTTACCTTGGACTCCTTGAAA
  CTGCCAGATGGGTACACACCCCGGAGAGGTGACGTGGTCAATGCAGTGGTGGTGGAGAGCAGCCAGTCAT
  GCTATGTCTGGAGGGCACTTTGTATGACCCTAGTGAAGAGGCGAGACGCCGCCCCTGTTCATGAGGCCAC
  TCATTTCTATGGAACGATTTTGCTGAAGAACAAAGGTGATATTGAAGTTACACAGGTGACGCATTTTGGA
  ACCCTAAAGGAAGGAAGAAGTAAAACCATGGTGATCTGGATAGAGAATAAAGGAGACATTCCTCAAAACT
  TAGTCAGCTGTAAACTGGCTGGCTGGGATAAATCTAAACAATTCAGATTCCAAATGCTGGATAAAGACCA
  GATGTGCCCCGTGGTATCTTTTGTTTCTGTTCCTGAGAAGGAGAATTCATCAGATGAAAATATTAATTCA
  TTAAATAGCCACACAAAAAACAAAACCTCTCAGATGTCGGAGAGCAGTTTGGTGAACAACAGAGGAATCT
  CTCCAGGTGATTGTACCTGTAAAGGAGAAAATGGAGAAAAAGACAACATTCTATCAAGGAAGCAGATGAC
  AGAGCCTGAGCCTGGGGGGCTTGTCCCTCCAGGGGGAAAAACCTTCATTGTGGTCATCTGTGACGGAAAA
  AATCCTGGCCGCTGCAAGGAGCTCCTTTTGCTCTGTTTTTCCGATTTCCTAATTGGGCGATACCTTGAAG
  TAAATGTTATCAGTGGGGAGGAGTCACTAATTGCTGCGCGCGAACCATTTTCTTGGAAAAAGCTTAAAAG
  TTCACAAGCGTTAACATCCGCAAAAACTACAGTTGTTGTGACCGCACAGAAAAGGAACTCAAGACGACAA
  CTTCCAAGTTTTCTTCCCCAATATCCAATCCCAGATAGACTTAGAAAATGTGTGGAACAAAAAATTGACA
  TCCTGACTTTCCAGCCATTACTTGCAGAGCTTCTGAACATGTCAAATTACAAGGAGAAGTTTTCGACTTT
  GCTGTGGCTTGAGGAGATTTATGCAGAAATGGAACTGAAAGAGTATAACATGAGCGGGATCATCTTAAGA
  AGGAATGGGGATCTGCTGGTTCTGGAGGTCCCAGGGTTGGCCGAAGGGAGGCCTTCTCTCTACGCAGGTG
  ATAAACTGATTTTAAAAACTCAAGAGTACAATGGACATGCCATCGAATACATCAGCTACGTGACTGAGAT
  TCATGAAGAAGATGTAACTCTTAAAATTAATCCAGAATTTGAACAAGCCTATAACTTTGAACCTATGGAT
  GTGGAATTTACATATAATAGGACCACAAGCAGACGGTGTCACTTTGCACTTGAACACGTCATCCACTTAG
  GTGTAAAAGTGTTGTTTCCAGAAGAAATTATTTTACAGTCTCCACAAGTGACGGGAAATTGGAACCATGC
  ACAAGACACCAAAAGCAGTGGACAGTCCACCAGCAAAAAGAATAGGAAAACAATGACGGACCAAGCTGAG
  CATGGAACAGAGGAGAGGCGTGTTGGTGACAAGGACCTGCCGGTGCTGGCACCCTTTACTGCAGAGATGA
  GCGATTGGGTAGATGAAATTCAGACCCCTAAAGCAAGAAAGATGGAGTTTTTCAACCCAGTGCTAAATGA
  AAATCAGAAGTTAGCAGTTAAAAGGATTCTGAGTGGTGACTGCCGTCCCCTCCCGTATATTCTCTTTGGA
  CCTCCTGGTACTGGAAAGACAGTGACAATAATAGAGGCTGTTTTACAGGTACACTTTGCCTTGCCGGACA
  GTCGGATTTTAGTCTGTGCGCCCTCCAACAGTGCTGCTGACCTCGTGTGTCTGCGGCTGCACGAGAGCAA
  GGTGCTACAGCCGGCCACCATGGTCCGGGTGAACGCCACCTGCAGGTTCGAGGAGATAGTTATTGACGCC
  GTCAAACCGTATTGCAGAGACGGAGAAGACATCTGGAAAGCCTCACGCTTCCGGATAATCATCACCACAT
  GCAGCAGCTCAGGGCTGTTTTACCAAATAGGAGTGAGAGTTGGGCACTTCACTCACGTGTTTGTGGACGA
  GGCTGGGCAGGCAAGTGAGCCGGAATGCCTCATTCCTCTGGGGCTGATGTCGGACATCAGTGGCCAGATC
  GTGCTGGCAGGAGACCCCATGCAGCTCGGCCCAGTCATTAAGTCCAGACTCGCCATGGCCTATGGGCTGA
  ACGTGTCCTTTTTGGAACGGCTGATGTCTCGACCCGCGTACCAGAGGGACGAAAATGCTTTCGGTGCTTG
  TGGCGCACATAATCCCCTGTTGGTCACAAAGCTGGTGAAGAACTACCGGTCCCACGAGGCCCTGCTGATG
  CTGCCCTCACGGCTGTTCTACCACAGGGAACTCGAGGTCTGTGCGGACCCCACAGTGGTGACCTCCTTGC
  TGGGCTGGGAGAAGTTGCCTAAGAAAGGCTTCCCTCTCATCTTCCATGGTGTGCGGGGCAGCGAGGCACG
  GGAGGGAAAAAGCCCATCGTGGTTCAACCCGGCCGAGGCCGTCCAGGTCCTGCGCTACTGCTGCCTCCTG
  GCCCACAGCATCTCCAGTCAGGTGTCTGCCAGCGACATTGGCGTCATCACGCCCTACCGGAAGCAGGTGG
  AGAAAATCAGAATTCTTTTGCGTAATGTTGATCTGATGGATATAAAGGTTGGATCAGTAGAGGAGTTTCA
  AGGACAAGAGTATCTGGTCATCATCATTTCGACCGTACGGTCAAATGAAGATAGATTTGAAGATGATCGA
  TATTTTTTGGGTTTCTTGTCCAACTCAAAAAGATTTAATGTTGCAATCACCAGACCCAAAGCTTTGCTGA
  TAGTGCTGGGAAACCCCCATGTTCTCGTTCGAGACCCCTGTTTTGGTGCTTTGCTGGAATACAGTATTAC
  AAACGGTGTTTACATGGGATGCGATTTACCTCCTGCACTGCAGTCTCTGCAAAACTGTGGCGAGGGGGTG
  GCAGACCCCTCCTACCCAGTGGTGCCAGAATCCACAGGACCAGAGAAGCATCAGGAGCCCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219511 representing NM_018995
  Red=Cloning site Green=Tags(s)
  
  MLSLAAKLVAFFWRTADTPREEAGQLEPELAEGDTKLKTVRGVVTRYCSDYGMIDDMIYFSSDAVTSRVL
  LNVGQEVIAVVEENKVSNGLKAIRVEAVSDKWEDDSRNHGSPSDCGPRVLIGCVTSLVEGAGCISQTTYF
  SLESVCEGFEPCKGDWVEAEYRIRPGTWSSEATSVKPLRYKRVDKVCISSLCGRNGVLEESIFFTLDSLK
  LPDGYTPRRGDVVNAVVVESSQSCYVWRALCMTLVKRRDAAPVHEATHFYGTILLKNKGDIEVTQVTHFG
  TLKEGRSKTMVIWIENKGDIPQNLVSCKLAGWDKSKQFRFQMLDKDQMCPVVSFVSVPEKENSSDENINS
  LNSHTKNKTSQMSESSLVNNRGISPGDCTCKGENGEKDNILSRKQMTEPEPGGLVPPGGKTFIVVICDGK
  NPGRCKELLLLCFSDFLIGRYLEVNVISGEESLIAAREPFSWKKLKSSQALTSAKTTVVVTAQKRNSRRQ
  LPSFLPQYPIPDRLRKCVEQKIDILTFQPLLAELLNMSNYKEKFSTLLWLEEIYAEMELKEYNMSGIILR
  RNGDLLVLEVPGLAEGRPSLYAGDKLILKTQEYNGHAIEYISYVTEIHEEDVTLKINPEFEQAYNFEPMD
  VEFTYNRTTSRRCHFALEHVIHLGVKVLFPEEIILQSPQVTGNWNHAQDTKSSGQSTSKKNRKTMTDQAE
  HGTEERRVGDKDLPVLAPFTAEMSDWVDEIQTPKARKMEFFNPVLNENQKLAVKRILSGDCRPLPYILFG
  PPGTGKTVTIIEAVLQVHFALPDSRILVCAPSNSAADLVCLRLHESKVLQPATMVRVNATCRFEEIVIDA
  VKPYCRDGEDIWKASRFRIIITTCSSSGLFYQIGVRVGHFTHVFVDEAGQASEPECLIPLGLMSDISGQI
  VLAGDPMQLGPVIKSRLAMAYGLNVSFLERLMSRPAYQRDENAFGACGAHNPLLVTKLVKNYRSHEALLM
  LPSRLFYHRELEVCADPTVVTSLLGWEKLPKKGFPLIFHGVRGSEAREGKSPSWFNPAEAVQVLRYCCLL
  AHSISSQVSASDIGVITPYRKQVEKIRILLRNVDLMDIKVGSVEEFQGQEYLVIIISTVRSNEDRFEDDR
  YFLGFLSNSKRFNVAITRPKALLIVLGNPHVLVRDPCFGALLEYSITNGVYMGCDLPPALQSLQNCGEGV
  ADPSYPVVPESTGPEKHQEPS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018995
• ORF Size: 3633 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018995.3
• RefSeq Size: 3910 bp
• RefSeq ORF: 3636 bp
• Locus ID: 54456
• UniProt ID: Q9BXT6
• Cytogenetics: 22q13.33
• MW: 135.1 kDa
• Gene Summary: This gene is similar to a mouse gene that encodes a putative RNA helicase and shows testis-specific expression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]