FNIP2 (NM_020840) Human Tagged ORF Clone

CAT#: RC219509

FNIP2 (Myc-DDK-tagged)-Human folliculin interacting protein 2 (FNIP2)

Product Images

Circular map for RC219509

• 

  Western blot validation of overexpression lysate (Cat# LY412262) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219509 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified FNIP2 protein (Cat# TP319509). The protein was produced from HEK293T cells transfected with FNIP2 cDNA clone (Cat# RC219509) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FNIP2
• Synonyms: FNIPL; MAPO1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219509 representing NM_020840
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCGACCCTGCTCCAGAAGCTCTTCAACAAAAGGGGCAGCAGCGGCAGCTCCGCGGCGGCGTCTG
  CCCAGGGCAGGGCTCCTAAGGAAGGACCCGCCTTTAGTTGGTCATGTTCGGAGTTTGACCTGAATGAGAT
  TCGCCTGATAGTTTACCAGGACTGTGACAGGAGAGGCAGACAAGTCTTGTTTGACTCTAAAGCTGTTCAA
  AAGATTGAGGAGGTGACAGCTCAGAAAACAGAGGATGTTCCTATTAAAATATCAGCCAAGTGCTGCCAGG
  GAAGCAGCAGTGTCAGCAGCAGTAGCAGCAGCAGCATCTCTTCCCACAGTTCTTCTGGGGGATCTTCACA
  TCATGCTAAGGAACAGCTTCCAAAGTACCAGTACACAAGACCAGCTTCCGATGTCAACATGTTAGGGGAA
  ATGATGTTTGGCTCAGTTGCCATGAGTTACAAAGGCTCCACCTTAAAGATACACTACATACGTTCTCCTC
  CACAACTGATGATTAGTAAAGTCTTCTCTGCTAGAATGGGCAGCTTCTGTGGAAGTACAAATAACTTGCA
  AGACAGCTTTGAGTACATCAACCAAGATCCTAATTTGGGAAAACTGAACACAAATCAAAATAGTTTGGGT
  CCTTGTCGTACTGGAAGTAACCTAGCACACAGCACACCAGTTGATATGCCAAGCAGAGGACAGAATGAAG
  ACAGGGACAGTGGCATTGCTCGATCAGCCTCACTAAGCAGTCTTTTGATCACACCTTTCCCATCTCCAAG
  CTCCTCTACATCTTCTTCCAGCAGTTACCAGCGCCGCTGGCTTCGAAGTCAGACAACAAGTTTGGAAAAT
  GGCATCATCCCAAGAAGGTCAACTGATGAGACATTCAGCTTGGCTGAAGAAACCTGTAGCTCTAATCCAG
  CTATGGTTAGGAGGAAGAAAATTGCCATAAGCATCATCTTTTCCCTATGTGAGAAAGAAGAAGCACAAAG
  GAATTTCCAGGACTTCTTCTTTTCTCATTTTCCCCTGTTTGAATCTCACATGAACAGGCTGAAGAGTGCA
  ATTGAAAAGGCTATGATCTCCTGTAGGAAAATAGCAGAATCAAGTCTCCGAGTCCAGTTTTATGTCAGCC
  GTTTGATGGAAGCTCTGGGAGAATTCAGAGGAACTATCTGGAACTTATATTCTGTTCCAAGGATAGCTGA
  ACCTGTATGGCTTACTATGATGTCCGGCACTTTGGAAAAAAACCAGCTCTGCCAGCGCTTTCTCAAGGAG
  TTTACACTTCTGATAGAACAGATAAATAAAAACCAGTTTTTTGCTGCCTTACTGACTGCGGTGTTAACCT
  ACCACCTGGCCTGGGTCCCAACTGTCATGCCTGTGGATCACCCTCCCATCAAAGCCTTCTCAGAGAAACG
  TACCTCCCAGTCAGTGAACATGCTGGCCAAAACACATCCGTATAATCCTCTTTGGGCACAGCTGGGTGAT
  CTTTACGGAGCCATAGGCTCTCCAGTGAGACTGACTCGCACCGTAGTGGTAGGGAAGCAGAAGGACTTAG
  TCCAGCGAATACTTTATGTCCTGACCTACTTTCTCCGTTGCTCTGAGCTACAAGAGAACCAGCTGACCTG
  GAGTGGCAATCATGGTGAAGGTGACCAAGTTTTAAATGGGAGCAAGATCATAACAGCCTTGGAGAAAGGA
  GAGGTGGAGGAGTCTGAGTATGTGGTCATTACGGTGAGGAACGAGCCCGCTCTTGTACCCCCCATCCTAC
  CACCAACAGCAGCAGAGAGACACAACCCCTGGCCGACAGGGTTTCCTGAGTGCCCAGAGGGCACTGACAG
  TAGAGACCTGGGTCTTAAACCTGACAAAGAAGCTAACAGGAGGCCAGAGCAGGGTTCTGAGGCTTGCAGC
  GCAGGGTGCCTGGGGCCAGCATCAGACGCTTCCTGGAAACCTCAGAATGCATTTTGTGGGGATGAGAAAA
  ATAAAGAGGCACCGCAAGATGGCTCTTCAAGACTTCCCAGCTGTGAAGTTTTGGGGGCAGGAATGAAGAT
  GGACCAGCAAGCTGTCTGTGAGCTGTTGAAAGTGGAGATGCCTACAAGACTGCCAGACCGGTCAGTGGCC
  TGGCCTTGCCCTGACAGACATCTCCGGGAGAAACCTTCCTTAGAAAAGGTCACTTTCCAGATTGGAAGCT
  TTGCATCTCCAGAGTCTGACTTTGAAAGCCGCATGAAAAAAATGGAGGAACGGGTGAAGGCCTGTGGCCC
  CTCCTTGGAGGCCAGTGAGGCTGCTGATGTGGCTCAGGACCCGCAGGTTTCTAGGAGCCCTTTTAAACCT
  GGCTTTCAGGAGAATGTTTGCTGTCCTCAGAATCGGCTTTCAGAGGGGGATGAAGGCGAGTCTGACAAGG
  GTTTTGCAGAGGACAGAGGCAGCAGAAACGACATGGCAGCAGATATTGCTGGGCAGCTCAGCCACGCTGC
  TGACTTGGGCACAGCCTCCCACGGTGCAGGAGGAACGGGAGGGAGGAGGCTGGAGGCCACTAGAGGTTTG
  TATGTGAAGGCTGCGGAAGGACCTGTGCTGGAGCCTGTTGCCCCCAGGTGTGTCCAGCGGGGCCCTGGCC
  TCGTGGCTGGTGCGAATATCCCCTGTGGGGATGACAACAAGAAGGCCAACTTCAGGACTGAAGGAGACAT
  TCCCCGAAATGAAAGCTCAGATAGCGCCCTGGGAGACAGTGACGACGAAGCCTGCGCTTCAGCCATGCTA
  GATCTGGGTCACGGTGGTGACAGGACTGGAGGGTCCTTGGAAGTGGAGCTGCCTCTGCCAAGGTCTCAGA
  GCATCAGCACCCAGAATGTAAGGAACTTTGGCCGCTCACTTCTGGCGGGCTACTGCCCCACATACATGCC
  TGATCTTGTGCTTCATGGGACCGGCAGTGATGAGAAGCTGAAGCAGTGCCTGGTGGCCGACCTTGTCCAC
  ACAGTCCATCATCCAGTCCTGGATGAGCCAATAGCTGAAGCTGTCTGTATTATCGCAGACACGGATAAAT
  GGAGTGTGCAGGTAGCTACAAGTCAGAGGAAAGTGACGGACAACATGAAACTAGGCCAGGATGTCCTGGT
  CTCTAGTCAGGTGTCCAGTTTGCTTCAGTCCATTTTACAGCTCTATAAGCTTCACCTCCCTGCTGATTTT
  TGCATCATGCATCTTGAAGATAGACTACAGGAGATGTACCTTAAAAGTAAAATGCTATCTGAATATCTCC
  GGGGACACACACGAGTCCATGTGAAAGAATTAGGTGTCGTACTGGGGATTGAATCCAACGACCTGCCTCT
  GTTGACTGCTATTGCCAGTACTCATTCTCCTTATGTGGCTCAAATACTCTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219509 representing NM_020840
  Red=Cloning site Green=Tags(s)
  
  MAPTLLQKLFNKRGSSGSSAAASAQGRAPKEGPAFSWSCSEFDLNEIRLIVYQDCDRRGRQVLFDSKAVQ
  KIEEVTAQKTEDVPIKISAKCCQGSSSVSSSSSSSISSHSSSGGSSHHAKEQLPKYQYTRPASDVNMLGE
  MMFGSVAMSYKGSTLKIHYIRSPPQLMISKVFSARMGSFCGSTNNLQDSFEYINQDPNLGKLNTNQNSLG
  PCRTGSNLAHSTPVDMPSRGQNEDRDSGIARSASLSSLLITPFPSPSSSTSSSSSYQRRWLRSQTTSLEN
  GIIPRRSTDETFSLAEETCSSNPAMVRRKKIAISIIFSLCEKEEAQRNFQDFFFSHFPLFESHMNRLKSA
  IEKAMISCRKIAESSLRVQFYVSRLMEALGEFRGTIWNLYSVPRIAEPVWLTMMSGTLEKNQLCQRFLKE
  FTLLIEQINKNQFFAALLTAVLTYHLAWVPTVMPVDHPPIKAFSEKRTSQSVNMLAKTHPYNPLWAQLGD
  LYGAIGSPVRLTRTVVVGKQKDLVQRILYVLTYFLRCSELQENQLTWSGNHGEGDQVLNGSKIITALEKG
  EVEESEYVVITVRNEPALVPPILPPTAAERHNPWPTGFPECPEGTDSRDLGLKPDKEANRRPEQGSEACS
  AGCLGPASDASWKPQNAFCGDEKNKEAPQDGSSRLPSCEVLGAGMKMDQQAVCELLKVEMPTRLPDRSVA
  WPCPDRHLREKPSLEKVTFQIGSFASPESDFESRMKKMEERVKACGPSLEASEAADVAQDPQVSRSPFKP
  GFQENVCCPQNRLSEGDEGESDKGFAEDRGSRNDMAADIAGQLSHAADLGTASHGAGGTGGRRLEATRGL
  YVKAAEGPVLEPVAPRCVQRGPGLVAGANIPCGDDNKKANFRTEGDIPRNESSDSALGDSDDEACASAML
  DLGHGGDRTGGSLEVELPLPRSQSISTQNVRNFGRSLLAGYCPTYMPDLVLHGTGSDEKLKQCLVADLVH
  TVHHPVLDEPIAEAVCIIADTDKWSVQVATSQRKVTDNMKLGQDVLVSSQVSSLLQSILQLYKLHLPADF
  CIMHLEDRLQEMYLKSKMLSEYLRGHTRVHVKELGVVLGIESNDLPLLTAIASTHSPYVAQILL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020840
• ORF Size: 3342 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020840.3
• RefSeq Size: 5801 bp
• RefSeq ORF: 3345 bp
• Locus ID: 57600
• UniProt ID: Q9P278
• Cytogenetics: 4q32.1
• MW: 121.9 kDa
• Gene Summary: This gene encodes a protein that binds to the tumor suppressor folliculin and to AMP-activated protein kinase (AMPK), and may play a role cellular metabolism and nutrient sensing by regulating the AMPK-mechanistic target of rapamycin signaling pathway. The encoded protein may also be involved in regulating the O6-methylguanine-induced apoptosis signaling pathway. This gene has a closely related paralog that encodes a protein with similar binding activities. Both related proteins also associate with the molecular chaperone heat shock protein-90 (Hsp90) and negatively regulate its ATPase activity and facilitate its association with folliculin. [provided by RefSeq, Jul 2017]