DAXX (NM_001350) Human Tagged ORF Clone

CAT#: RC219497

DAXX (Myc-DDK-tagged)-Human death-domain associated protein (DAXX), transcript variant 2

Product Images

Circular map for RC219497

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  Western blot validation of overexpression lysate (Cat# LY427986) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226603 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified DAXX protein (Cat# TP319497). The protein was produced from HEK293T cells transfected with DAXX cDNA clone (Cat# RC219497) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DAXX
• Synonyms: BING2; DAP6; EAP1; SMIM40
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219497 representing NM_001350
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACCGCTAACAGCATCATCGTGCTGGATGATGATGACGAAGATGAAGCAGCTGCTCAGCCAGGGC
  CCTCCCACCCACTCCCCAATGCGGCCTCACCTGGGGCAGAAGCCCCTAGCTCCTCTGAGCCTCATGGGGC
  CAGAGGAAGCAGTAGTTCGGGCGGCAAGAAATGCTACAAGCTGGAGAATGAGAAGCTGTTCGAAGAGTTC
  CTTGAACTTTGTAAGATGCAGACAGCAGACCACCCTGAGGTGGTCCCATTCCTCTATAACCGGCAGCAAC
  GTGCCCACTCTCTGTTTTTGGCCTCGGCGGAGTTCTGCAACATCCTCTCTAGGGTCCTGTCTCGGGCCCG
  GAGCCGGCCAGCCAAGCTCTATGTCTACATCAATGAGCTCTGCACTGTTCTCAAGGCCCACTCAGCCAAA
  AAGAAGCTGAACTTGGCCCCTGCCGCCACCACCTCCAATGAGCCCTCTGGGAATAACCCTCCCACACACC
  TCTCCTTGGACCCCACAAATGCTGAAAACACTGCCTCTCAGTCTCCAAGGACCCGTGGTTCCCGGCGGCA
  GATCCAGCGTTTGGAGCAGCTGCTGGCGCTCTATGTGGCAGAGATCCGGCGGCTGCAGGAAAAGGAGTTG
  GATCTCTCAGAATTGGATGACCCAGACTCCGCATACCTGCAGGAGGCACGGTTGAAGCGTAAGCTGATCC
  GCCTCTTTGGGCGACTATGTGAGCTGAAAGACTGCTCTTCACTGACCGGCCGTGTCATAGAGCAGCGCAT
  CCCCTACCGTGGCACCCGCTACCCAGAGGTTAACAGGCGCATTGAGCGGCTCATCAACAAGCCAGGGCCT
  GATACCTTCCCTGACTATGGGGATGTGCTTCGGGCTGTAGAGAAGGCAGCTGCCCGACACAGCCTTGGCC
  TCCCCCGACAGCAGCTCCAGCTCATGGCTCAGGATGCCTTCCGAGATGTGGGCATCAGGTTACAGGAGCG
  ACGTCACCTCGATCTCATCTACAACTTTGGCTGCCACCTCACAGATGACTATAGGCCAGGCGTTGACCCT
  GCACTATCAGATCCTGTGTTGGCCCGGCGCCTTCGGGAAAACCGGAGTTTGGCCATGAGTCGGCTGGATG
  AGGTCATCTCCAAATATGCAATGTTGCAAGACAAAAGTGAGGAGGGCGAGAGAAAAAAGAGAAGAGCTCG
  GCTCCAAGGCACCTCTTCCCACTCTGCAGACACCCCCGAAGCCTCCTTGGATTCTGGTGAGGGCCCTAGT
  GGAATGGCATCCCAGGGGTGCCCTTCTGCCTCCAGAGCTGAGACAGATGACGAAGACGATGAGGAGAGTG
  ATGAGGAAGAGGAGGAGGAGGAGGAAGAAGAAGAGGAGGAGGCCACAGATTCTGAAGAGGAGGAGGATCT
  GGAACAGATGCAGGAGGGTCAGGAGGATGATGAAGAGGAGGACGAAGAGGAAGAAGCAGCAGCAGGTAAA
  GATGGAGACAAGAGCCCCATGTCCTCACTACAGATCTCCAATGAAAAGAACCTGGAACCTGGCAAACAGA
  TCAGCAGATCTTCAGGGGAGCAGCAAAACAAAGGACGCATAGTGTCACCATCGTTACTGTCAGAAGAACC
  CCTGGCCCCCTCCAGCATAGATGCTGAAAGCAATGGAGAACAGCCTGAGGAGCTGACCCTGGAGGAAGAA
  AGCCCTGTGTCTCAGCTCTTTGAGCTAGAGATTGAAGCTTTGCCCCTGGATACCCCTTCCTCTGTGGAGA
  CGGACATTTCCTCTTCCAGGAAGCAATCAGAGGAGCCCTTCACCACTGTCTTAGAGAATGGAGCAGGCAT
  GGTCTCTTCTACTTCCTTCAATGGAGGCGTCTCTCCTCACAACTGGGGAGATTCTGGTCCCCCCTGCAAA
  AAATCTCGGAAGGAGAAGAAGCAAACAGGATCAGGGCCATTAGGAAACAGCTATGTGGAAAGGCAAAGGT
  CAGTGCATGAGAAGAATGGGAAAAAGATATGTACCCTGCCCAGCCCACCTTCCCCCTTGGCTTCCTTGGC
  CCCAGTTGCTGATTCCTCCACGAGGGTGGACTCTCCCAGCCATGGCCTGGTGACCAGCTCCCTCTGCATC
  CCTTCTCCAGCCCGGCTGTCCCAAACCCCCCATTCACAGCCTCCTCGGCCTGGTACTTGCAAGACAAGTG
  TGGCCACACAATGCGATCCAGAAGAGATCATCGTGCTCTCAGACTCTGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219497 representing NM_001350
  Red=Cloning site Green=Tags(s)
  
  MATANSIIVLDDDDEDEAAAQPGPSHPLPNAASPGAEAPSSSEPHGARGSSSSGGKKCYKLENEKLFEEF
  LELCKMQTADHPEVVPFLYNRQQRAHSLFLASAEFCNILSRVLSRARSRPAKLYVYINELCTVLKAHSAK
  KKLNLAPAATTSNEPSGNNPPTHLSLDPTNAENTASQSPRTRGSRRQIQRLEQLLALYVAEIRRLQEKEL
  DLSELDDPDSAYLQEARLKRKLIRLFGRLCELKDCSSLTGRVIEQRIPYRGTRYPEVNRRIERLINKPGP
  DTFPDYGDVLRAVEKAAARHSLGLPRQQLQLMAQDAFRDVGIRLQERRHLDLIYNFGCHLTDDYRPGVDP
  ALSDPVLARRLRENRSLAMSRLDEVISKYAMLQDKSEEGERKKRRARLQGTSSHSADTPEASLDSGEGPS
  GMASQGCPSASRAETDDEDDEESDEEEEEEEEEEEEEATDSEEEEDLEQMQEGQEDDEEEDEEEEAAAGK
  DGDKSPMSSLQISNEKNLEPGKQISRSSGEQQNKGRIVSPSLLSEEPLAPSSIDAESNGEQPEELTLEEE
  SPVSQLFELEIEALPLDTPSSVETDISSSRKQSEEPFTTVLENGAGMVSSTSFNGGVSPHNWGDSGPPCK
  KSRKEKKQTGSGPLGNSYVERQRSVHEKNGKKICTLPSPPSPLASLAPVADSSTRVDSPSHGLVTSSLCI
  PSPARLSQTPHSQPPRPGTCKTSVATQCDPEEIIVLSDSD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001350
• ORF Size: 2220 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001350.5
• RefSeq Size: 2477 bp
• RefSeq ORF: 2223 bp
• Locus ID: 1616
• UniProt ID: Q9UER7
• Cytogenetics: 6p21.32
• Domains: Daxx
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), MAPK signaling pathway
• MW: 81.2 kDa
• Gene Summary: This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008]