TAF1 (NM_004606) Human Tagged ORF Clone

CAT#: RC219444

TAF1 (Myc-DDK-tagged)-Human TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa (TAF1), transcript variant 1

Product Images

Circular map for RC219444

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TAF1
• Synonyms: BA2R; CCG1; CCGS; DYT3; DYT3/TAF1; KAT4; MRXS33; N-TAF1; NSCL2; OF; P250; TAF(II)250; TAF2A; TAFII-250; TAFII250; XDP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219444 representing NM_004606
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGACCCGGCTGCGATTTGCTGCTGCGGACAGCAGCTACCATCACTGCTGCCGCCATCATGTCAGACA
  CGGACAGCGACGAAGATTCCGCTGGAGGCGGCCCATTTTCTTTAGCGGGTTTCCTTTTCGGCAACATCAA
  TGGAGCCGGGCAGCTGGAGGGGGAAAGCGTCTTGGATGATGAATGTAAGAAGCACTTGGCAGGCTTGGGG
  GCTTTGGGGCTGGGCAGCCTGATCACTGAACTCACGGCAAATGAAGAATTGACCGGGACTGACGGTGCCT
  TGGTAAATGATGAAGGGTGGGTTAGGAGTACAGAAGATGCTGTGGACTATTCAGACATCAATGAGGTGGC
  AGAAGATGAAAGCCGAAGATACCAGCAGACGATGGGGAGCTTGCAGCCCCTTTGCCACTCAGATTATGAT
  GAAGATGACTATGATGCTGATTGTGAAGACATTGATTGCAAGTTGATGCCTCCTCCACCTCCACCCCCGG
  GACCAATGAAGAAGGATAAGGACCAGGATTCTATTACTGGTGTGTCTGAAAATGGAGAAGGCATCATCTT
  GCCCTCCATCATTGCCCCTTCCTCTTTGGCCTCAGAGAAAGTGGACTTCAGTAGTTCCTCTGACTCAGAA
  TCTGAGATGGGACCTCAGGAAGCAACACAGGCAGAATCTGAAGATGGAAAGCTGACCCTTCCATTGGCTG
  GGATTATGCAGCATGATGCCACCAAGCTGTTGCCAAGTGTCACAGAACTTTTTCCAGAATTTCGACCTGG
  AAAGGTGTTACGTTTTCTACGTCTTTTTGGACCAGGGAAGAATGTCCCATCTGTTTGGCGGAGTGCTCGG
  AGAAAGAGGAAGAAGAAGCACCGTGAGCTGATACAGGAAGAGCAGATCCAGGAGGTGGAGTGCTCAGTAG
  AATCAGAAGTCAGCCAGAAGTCTTTGTGGAACTACGACTACGCTCCACCACCACCTCCAGAGCAGTGTCT
  CTCTGATGATGAAATCACGATGATGGCTCCTGTGGAGTCCAAATTTTCCCAATCAACTGGAGATATAGAT
  AAAGTGACAGATACCAAACCAAGAGTGGCTGAGTGGCGTTATGGGCCTGCCCGACTGTGGTATGATATGC
  TGGGTGTCCCTGAAGATGGCAGTGGGTTTGACTATGGCTTCAAACTGAGAAAGACAGAACATGAACCTGT
  GATAAAATCTAGAATGATAGAGGAATTTAGGAAACTTGAGGAAAACAATGGCACTGATCTTCTGGCTGAT
  GAAAACTTCCTGATGGTGACACAGCTGCATTGGGAGGATGATATCATCTGGGATGGGGAGGATGTCAAAC
  ACAAAGGGACAAAACCTCAGCGTGCAAGCCTGGCAGGCTGGCTTCCTTCTAGCATGACTAGGAATGCGAT
  GGCTTACAATGTTCAGCAAGGTTTTGCAGCCACTCTTGATGATGACAAACCTTGGTACTCCATTTTTCCC
  ATTGACAATGAGGATCTGGTATATGGACGCTGGGAGGACAATATCATTTGGGATGCTCAGGCCATGCCCC
  GGCTGTTGGAACCTCCTGTTTTGACACTTGATCCCAATGATGAGAACCTCATTTTGGAAATTCCTGATGA
  GAAGGAAGAGGCCACCTCTAACTCCCCCTCCAAGGAGAGTAAGAAGGAATCATCTCTGAAGAAGAGTCGA
  ATTCTCTTAGGGAAAACAGGAGTCATCAAGGAGGAACCACAGCAGAACATGTCTCAGCCAGAAGTGAAAG
  ATCCATGGAATCTCTCCAATGATGAGTATTATTATCCCAAGCAACAGGGTCTTCGAGGCACCTTTGGAGG
  GAATATTATCCAGCATTCAATTCCTGCTGTGGAATTACGGCAGCCCTTCTTTCCCACCCACATGGGGCCC
  ATCAAACTCCGGCAGTTCCATCGCCCACCTCTGAAAAAGTACTCATTTGGTGCACTTTCTCAGCCAGGTC
  CCCACTCAGTCCAACCTTTGCTAAAGCACATCAAAAAAAAGGCCAAGATGAGAGAACAAGAGAGGCAAGC
  TTCAGGTGGTGGAGAGATGTTTTTTATGCGCACACCTCAGGACCTCACAGGCAAAGATGGTGATCTTATT
  CTTGCAGAATATAGTGAGGAAAATGGACCCTTAATGATGCAGGTTGGCATGGCAACCAAGATAAAGAACT
  ATTATAAACGGAAACCTGGAAAAGATCCTGGAGCACCAGATTGTAAATATGGGGAAACTGTTTACTGCCA
  TACATCTCCTTTCCTGGGTTCTCTCCATCCTGGCCAATTGCTGCAAGCATTTGAGAACAACCTTTTTCGT
  GCTCCAATTTATCTTCATAAGATGCCAGAAACTGATTTCTTGATCATTCGGACAAGACAGGGTTACTATA
  TTCGGGAATTAGTGGATATTTTTGTGGTTGGCCAGCAGTGTCCCTTGTTTGAAGTTCCTGGGCCTAACTC
  CAAAAGGGCCAATACGCATATTCGAGACTTTCTACAGGTTTTTATTTACCGCCTTTTCTGGAAAAGTAAA
  GATCGGCCACGGAGGATACGAATGGAAGATATAAAAAAAGCCTTTCCTTCCCATTCAGAAAGCAGCATCC
  GGAAGAGGCTAAAGCTCTGCGCTGACTTCAAACGCACAGGGATGGACTCAAACTGGTGGGTGCTTAAGTC
  TGATTTTCGTTTACCAACGGAAGAAGAGATCAGAGCTATGGTGTCACCAGAGCAGTGCTGTGCTTATTAT
  AGCATGATAGCTGCAGAGCAACGACTGAAGGATGCTGGCTATGGTGAGAAATCCTTTTTTGCTCCAGAAG
  AAGAAAATGAGGAAGATTTCCAGATGAAGATTGATGATGAAGTTCGCACTGCCCCTTGGAACACCACAAG
  GGCCTTCATTGCTGCCATGAAGGGCAAGTGTCTGCTAGAGGTGACTGGGGTGGCAGATCCCACGGGGTGT
  GGTGAAGGATTCTCCTATGTGAAGATTCCAAACAAACCAACACAGCAGAAGGATGATAAAGAACCGCAGC
  CAGTGAAGAAGACAGTGACAGGAACAGATGCAGACCTTCGTCGCCTTTCCCTGAAAAATGCCAAGCAACT
  TCTACGTAAATTTGGTGTGCCTGAGGAAGAGATTAAAAAGTTGTCCCGCTGGGAAGTGATTGATGTGGTG
  CGCACAATGTCAACAGAACAGGCTCGTTCTGGAGAGGGGCCCATGAGTAAATTTGCCCGTGGATCAAGGT
  TTTCTGTGGCTGAGCATCAAGAGCGTTACAAAGAGGAATGTCAGCGCATCTTTGACCTACAGAACAAGGT
  TCTGTCATCAACTGAAGTCTTATCAACTGACACAGACAGCAGCTCAGCTGAAGATAGTGACTTTGAAGAA
  ATGGGAAAGAACATTGAGAACATGTTGCAGAACAAGAAAACCAGCTCTCAGCTTTCACGTGAACGGGAGG
  AACAGGAGCGGAAGGAACTACAGCGAATGCTACTGGCAGCAGGCTCAGCAGCATCCGGAAACAATCACAG
  AGATGATGACACAGCTTCCGTGACTAGCCTTAACTCTTCTGCCACTGGACGCTGTCTCAAGATTTATCGC
  ACGTTTCGAGATGAAGAGGGGAAAGAGTATGTTCGCTGTGAGACAGTCCGAAAACCAGCTGTCATTGATG
  CCTATGTGCGCATACGGACTACAAAAGATGAGGAATTCATTCGAAAATTTGCCCTTTTTGATGAACAACA
  TCGGGAAGAGATGCGAAAAGAACGGCGGAGGATTCAAGAGCAACTGAGGCGGCTTAAGAGGAACCAGGAA
  AAGGAGAAGCTTAAGGGTCCTCCTGAGAAGAAGCCCAAGAAAATGAAGGAGCGTCCTGACCTAAAACTGA
  AATGTGGGGCATGTGGTGCCATTGGACACATGAGGACTAACAAATTCTGCCCCCTCTATTATCAAACAAA
  TGCGCCACCTTCCAACCCTGTTGCCATGACAGAAGAACAGGAGGAGGAGTTGGAAAAGACAGTCATTCAT
  AATGATAATGAAGAACTTATCAAGGTTGAAGGGACCAAAATTGTCTTGGGGAAACAGCTAATTGAGAGTG
  CGGATGAGGTTCGCAGAAAATCTCTGGTTCTCAAGTTTCCTAAACAGCAGCTTCCTCCAAAGAAGAAACG
  GCGAGTTGGAACCACTGTTCACTGTGACTATTTGAATAGACCTCATAAGTCCATCCACCGGCGCCGCACA
  GACCCTATGGTGACGCTGTCGTCCATCTTGGAGTCTATCATCAATGACATGAGAGATCTTCCAAATACAT
  ACCCTTTCCACACTCCAGTCAATGCAAAGGTTGTAAAGGACTACTACAAAATCATCACTCGGCCAATGGA
  CCTACAAACACTCCGCGAAAACGTGCGTAAACGCCTCTACCCATCTCGGGAAGAGTTCAGAGAGCATCTG
  GAGCTAATTGTGAAAAATAGTGCAACCTACAATGGGCCAAAACACTCATTGACTCAGATCTCTCAATCCA
  TGCTGGATCTCTGTGATGAAAAACTCAAAGAGAAAGAAGACAAATTAGCTCGCTTAGAGAAAGCTATCAA
  CCCCTTGCTGGATGATGATGACCAAGTGGCGTTTTCTTTCATTCTGGACAACATTGTCACCCAGAAAATG
  ATGGCAGTTCCAGATTCTTGGCCATTTCATCACCCAGTTAATAAGAAATTTGTTCCAGATTATTACAAAG
  TGATTGTCAATCCAATGGATTTAGAGACCATACGTAAGAACATCTCCAAGCACAAGTATCAGAGTCGGGA
  GAGCTTTCTGGATGATGTAAACCTTATTCTGGCCAACAGTGTTAAGTATAATGGACCTGAGAGTCAGTAT
  ACTAAGACTGCCCAGGAGATTGTGAACGTCTGTTACCAGACATTGACTGAGTATGATGAACATTTGACTC
  AACTTGAGAAGGATATTTGTACTGCTAAAGAAGCAGCTTTGGAGGAAGCAGAATTAGAAAGCCTGGACCC
  AATGACCCCAGGGCCCTACACGCCTCAGCCTCCTGATTTGTATGATACCAACACATCCCTCAGTATGTCT
  CGAGATGCCTCTGTATTTCAAGATGAGAGCAATATGTCTGTCTTGGATATTCCCAGTGCCACTCCAGAAA
  AGCAGGTAACACAGGAAGGTGAAGATGGAGATGGTGATCTTGCAGATGAAGAGGAAGGAACTGTACAACA
  GCCTCAAGCCAGTGTCCTGTATGAGGATTTGCTTATGTCTGAAGGAGAAGATGATGAGGAAGATGCTGGG
  AGTGATGAAGAAGGAGACAATCCTTTCTCTGCTATCCAGCTGAGTGAAAGTGGAAGTGACTCTGATGTGG
  GATCTGGTGGAATAAGACCCAAACAACCCCGCATGCTTCAGGAGAACACAAGGATGGACATGGAAAATGA
  AGAAAGCATGATGTCCTATGAGGGAGACGGTGGGGAGGCTTCCCATGGTTTGGAGGATAGCAACATCAGT
  TATGGGAGCTATGAGGAGCCTGATCCCAAGTCGAACACCCAAGACACAAGCTTCAGCAGCATCGGTGGGT
  ATGAGGTATCAGAGGAGGAAGAAGATGAGGAGGAGGAAGAGCAGCGCTCTGGGCCGAGCGTACTAAGCCA
  GGTCCACCTGTCAGAGGACGAGGAGGACAGTGAGGATTTCCACTCCATTGCTGGGGACAGTGACTTGGAC
  TCTGATGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219444 representing NM_004606
  Red=Cloning site Green=Tags(s)
  
  MGPGCDLLLRTAATITAAAIMSDTDSDEDSAGGGPFSLAGFLFGNINGAGQLEGESVLDDECKKHLAGLG
  ALGLGSLITELTANEELTGTDGALVNDEGWVRSTEDAVDYSDINEVAEDESRRYQQTMGSLQPLCHSDYD
  EDDYDADCEDIDCKLMPPPPPPPGPMKKDKDQDSITGVSENGEGIILPSIIAPSSLASEKVDFSSSSDSE
  SEMGPQEATQAESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSAR
  RKRKKKHRELIQEEQIQEVECSVESEVSQKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDID
  KVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKTEHEPVIKSRMIEEFRKLEENNGTDLLAD
  ENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFP
  IDNEDLVYGRWEDNIIWDAQAMPRLLEPPVLTLDPNDENLILEIPDEKEEATSNSPSKESKKESSLKKSR
  ILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGP
  IKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLI
  LAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFR
  APIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSK
  DRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYY
  SMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGC
  GEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVV
  RTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEVLSTDTDSSSAEDSDFEE
  MGKNIENMLQNKKTSSQLSREREEQERKELQRMLLAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYR
  TFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQE
  KEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIH
  NDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRT
  DPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHL
  ELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKM
  MAVPDSWPFHHPVNKKFVPDYYKVIVNPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQY
  TKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQPPDLYDTNTSLSMS
  RDASVFQDESNMSVLDIPSATPEKQVTQEGEDGDGDLADEEEGTVQQPQASVLYEDLLMSEGEDDEEDAG
  SDEEGDNPFSAIQLSESGSDSDVGSGGIRPKQPRMLQENTRMDMENEESMMSYEGDGGEASHGLEDSNIS
  YGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEEEQRSGPSVLSQVHLSEDEEDSEDFHSIAGDSDLD
  SDE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004606
• ORF Size: 5679 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004606.4
• RefSeq Size: 6137 bp
• RefSeq ORF: 5622 bp
• Locus ID: 6872
• UniProt ID: P21675
• Cytogenetics: Xq13.1
• Protein Families: Protein Kinase
• Protein Pathways: Basal transcription factors
• MW: 214.5 kDa
• Gene Summary: Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N- and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. Mutations in this gene result in Dystonia 3, torsion, X-linked, a dystonia-parkinsonism disorder. Alternative splicing of this gene results in multiple transcript variants. This gene is part of a complex transcription unit (TAF1/DYT3), wherein some transcript variants share exons with TAF1 as well as additional downstream DYT3 exons. [provided by RefSeq, Oct 2013]