AGXT2 (NM_031900) Human Tagged ORF Clone

CAT#: RC219357

AGXT2 (Myc-DDK-tagged)-Human alanine--glyoxylate aminotransferase 2 (AGXT2), nuclear gene encoding mitochondrial protein

Product Images

Circular map for RC219357

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  Western blot validation of overexpression lysate (Cat# LY410447) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219357 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified AGXT2 protein (Cat# TP319357). The protein was produced from HEK293T cells transfected with AGXT2 cDNA clone (Cat# RC219357) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AGXT2
• Synonyms: AGT2; BAIBA; DAIBAT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219357 representing NM_031900
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTCTAATCTGGAGACATTTGCTGAGACCCTTGTGCCTGGTCACTTCCGCTCCCAGGATCCTTGAGA
  TGCATCCTTTCCTGAGCCTAGGTACTTCCCGGACATCAGTAACCAAGCTCAGTCTTCATACAAAGCCCAG
  AATGCCTCCATGTGACTTCATGCCTGAAAGATACCAGTCCCTTGGCTACAACCGTGTCCTGGAAATCCAC
  AAGGAACATCTTTCTCCTGTGGTGACGGCATATTTCCAGAAACCCCTGCTGCTCCACCAGGGGCACATGG
  AGTGGCTCTTTGATGCTGAAGGAAGCAGATACCTGGATTTCTTTTCCGGGATTGTTACTGTCAGTGTTGG
  CCATTGCCACCCAAAGGTGAATGCAGTGGCACAAAAGCAGCTCGGCCGCCTGTGGCATACAAGCACCGTC
  TTCTTCCACCCTCCAATGCATGAATATGCAGAGAAGCTTGCCGCACTTCTTCCTGAGCCTCTTAAGGTCA
  TTTTCTTGGTGAACAGTGGCTCAGAAGCCAATGAGCTGGCCATGCTGATGGCCAGGGCGCACTCAAACAA
  CATAGACATCATTTCTTTCAGAGGAGCCTACCATGGATGCAGTCCTTACACACTTGGCTTGACAAACGTA
  GGGATCTACAAGATGGAACTCCCTGGTGGGACAGGTTGCCAACCAACAATGTGTCCAGATGTTTTTCGTG
  GCCCTTGGGGAGGAAGCCACTGTCGAGATTCTCCAGTGCAAACAATCAGGAAGTGCAGCTGTGCACCAGA
  CTGCTGCCAAGCTAAAGATCAGTATATTGAGCAATTCAAAGATACGCTGAGCACATCTGTGGCCAAGTCA
  ATTGCTGGATTTTTCGCAGAACCTATTCAAGGTGTGAATGGAGTTGTCCAGTACCCAAAGGGGTTTCTAA
  AGGAAGCCTTTGAGCTGGTGCGAGCAAGGGGAGGCGTGTGCATTGCAGATGAAGTGCAGACAGGATTTGG
  AAGGTTGGGCTCTCACTTCTGGGGCTTCCAAACCCACGATGTCCTGCCTGACATTGTCACCATGGCTAAA
  GGGATTGGGAATGGCTTTCCCATGGCAGCAGTCATAACCACTCCAGAGATTGCCAAATCTTTGGCGAAAT
  GCCTGCAGCACTTCAACACCTTTGGAGGGAACCCCATGGCCTGTGCCATTGGATCTGCTGTGCTTGAGGT
  GATTAAAGAAGAAAATCTACAGGAAAACAGTCAAGAAGTTGGGACCTACATGTTACTAAAGTTTGCTAAG
  CTGCGGGATGAATTTGAAATTGTTGGAGACGTCCGAGGCAAAGGCCTCATGATAGGCATAGAAATGGTGC
  AGGATAAGATAAGCTGTCGGCCTCTTCCCCGTGAAGAAGTAAATCAGATCCATGAGGACTGCAAGCACAT
  GGGACTCCTCGTTGGCAGAGGCAGCATTTTTTCTCAGACATTTCGCATTGCGCCCTCAATGTGCATCACT
  AAACCAGAAGTTGATTTTGCAGTAGAAGTATTTCGTTCTGCCTTAACCCAACACATGGAAAGAAGAGCTA
  AG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219357 representing NM_031900
  Red=Cloning site Green=Tags(s)
  
  MTLIWRHLLRPLCLVTSAPRILEMHPFLSLGTSRTSVTKLSLHTKPRMPPCDFMPERYQSLGYNRVLEIH
  KEHLSPVVTAYFQKPLLLHQGHMEWLFDAEGSRYLDFFSGIVTVSVGHCHPKVNAVAQKQLGRLWHTSTV
  FFHPPMHEYAEKLAALLPEPLKVIFLVNSGSEANELAMLMARAHSNNIDIISFRGAYHGCSPYTLGLTNV
  GIYKMELPGGTGCQPTMCPDVFRGPWGGSHCRDSPVQTIRKCSCAPDCCQAKDQYIEQFKDTLSTSVAKS
  IAGFFAEPIQGVNGVVQYPKGFLKEAFELVRARGGVCIADEVQTGFGRLGSHFWGFQTHDVLPDIVTMAK
  GIGNGFPMAAVITTPEIAKSLAKCLQHFNTFGGNPMACAIGSAVLEVIKEENLQENSQEVGTYMLLKFAK
  LRDEFEIVGDVRGKGLMIGIEMVQDKISCRPLPREEVNQIHEDCKHMGLLVGRGSIFSQTFRIAPSMCIT
  KPEVDFAVEVFRSALTQHMERRAK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031900
• ORF Size: 1542 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031900.4
• RefSeq Size: 2165 bp
• RefSeq ORF: 1545 bp
• Locus ID: 64902
• UniProt ID: Q9BYV1
• Cytogenetics: 5p13.2
• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism
• MW: 52.4 kDa
• Gene Summary: The protein encoded by this gene is a class III pyridoxal-phosphate-dependent mitochondrial aminotransferase. It catalyzes the conversion of glyoxylate to glycine using L-alanine as the amino donor. It is an important regulator of methylarginines and is involved in the control of blood pressure in kidney. Polymorphisms in this gene affect methylarginine and beta-aminoisobutyrate metabolism, and are associated with carotid atherosclerosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]