OSTA (SLC51A) (NM_152672) Human Tagged ORF Clone

CAT#: RC219313

SLC51A (Myc-DDK-tagged)-Human organic solute transporter alpha (OSTalpha)

Product Images

Circular map for RC219313

• 

  Western blot validation of overexpression lysate (Cat# LY403483) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219313 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: OSTA
• Synonyms: OSTA; OSTalpha
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219313 representing NM_152672
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCGGGCAGGACCCAGATAAAGCTTGACCCCAGGTACACAGCAGATCTTCTGGAGGTGCTGAAGA
  CCAATTACGGCATCCCCTCCGCCTGCTTCTCTCAGCCTCCCACAGCAGCCCAACTCCTGAGAGCCCTGGG
  CCCTGTGGAACTTGCCCTCACTAGCATCCTGACCTTGCTGGCGCTGGGCTCCATTGCCATCTTCCTGGAG
  GATGCCGTCTACCTGTACAAGAACACCCTTTGCCCCATCAAGAGGCGGACTCTGCTCTGGAAGAGCTCGG
  CACCCACGGTGGTGTCTGTGCTGTGCTGCTTTGGTCTCTGGATCCCTCGTTCCCTGGTGCTGGTGGAAAT
  GACCATCACCTCGTTTTATGCCGTGTGCTTTTACCTGCTGATGCTGGTCATGGTGGAAGGCTTTGGGGGG
  AAGGAGGCAGTGCTGAGGACGCTGAGGGACACCCCGATGATGGTCCACACAGGCCCCTGCTGCTGCTGCT
  GCCCCTGCTGTCCACGGCTGCTGCTCACCAGGAAGAAGCTTCAGCTGCTGATGTTGGGCCCTTTCCAATA
  CGCCTTCTTGAAGATAACGCTGACCCTGGTGGGCCTGTTTCTCATCCCCGACGGCATCTATGACCCAGCA
  GACATTTCTGAGGGGAGCACAGCTCTATGGATCAACACTTTCCTCGGCGTGTCCACACTGCTGGCTCTCT
  GGACCCTGGGCATCATTTCCCGTCAAGCCAGGCTACACCTGGGTGAGCAGAACATGGGAGCCAAATTTGC
  TCTGTTCCAGGTTCTCCTCATCCTGACTGCCCTACAGCCCTCCATCTTCTCAGTCTTGGCCAACGGTGGG
  CAGATTGCTTGTTCGCCTCCCTATTCCTCTAAAACCAGGTCTCAAGTGATGAATTGCCACCTCCTCATAC
  TGGAGACTTTTCTAATGACTGTGCTGACACGAATGTACTACCGAAGGAAAGACCACAAGGTTGGGTATGA
  AACTTTCTCTTCTCCAGACCTGGACTTGAACCTCAAAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219313 representing NM_152672
  Red=Cloning site Green=Tags(s)
  
  MEPGRTQIKLDPRYTADLLEVLKTNYGIPSACFSQPPTAAQLLRALGPVELALTSILTLLALGSIAIFLE
  DAVYLYKNTLCPIKRRTLLWKSSAPTVVSVLCCFGLWIPRSLVLVEMTITSFYAVCFYLLMLVMVEGFGG
  KEAVLRTLRDTPMMVHTGPCCCCCPCCPRLLLTRKKLQLLMLGPFQYAFLKITLTLVGLFLIPDGIYDPA
  DISEGSTALWINTFLGVSTLLALWTLGIISRQARLHLGEQNMGAKFALFQVLLILTALQPSIFSVLANGG
  QIACSPPYSSKTRSQVMNCHLLILETFLMTVLTRMYYRRKDHKVGYETFSSPDLDLNLKA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152672
• ORF Size: 1020 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152672.6
• RefSeq Size: 1476 bp
• RefSeq ORF: 1023 bp
• Locus ID: 200931
• UniProt ID: Q86UW1
• Cytogenetics: 3q29
• Protein Families: Transmembrane
• MW: 37.6 kDa
• Gene Summary: Essential component of the Ost-alpha/Ost-beta complex, a heterodimer that acts as the intestinal basolateral transporter responsible for bile acid export from enterocytes into portal blood. Efficiently transports the major species of bile acids.[UniProtKB/Swiss-Prot Function]