BCORL1 (NM_021946) Human Tagged ORF Clone

CAT#: RC219297

BCORL1 (Myc-DDK-tagged)-Human BCL6 corepressor-like 1 (BCORL1)

Product Images

Circular map for RC219297

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BCORL1
• Synonyms: BCoR-L1; CXorf10; SHUVER
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219297 representing NM_021946
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCTCTACAGCACCGCTCTACAGCGGCGTGCACAACTGGACCAGTTCTGACCGGATTCGCATGTGTG
  GCATCAACGAGGAGAGAAGAGCACCTCTTTCTGATGAGGAGTCAACGACAGGCGACTGCCAGCACTTTGG
  ATCTCAGGAGTTTTGTGTCAGCAGCAGTTTTTCCAAGGTGGAGCTCACGGCAGTTGGAAGTGGCAGCAAT
  GCCCGGGGGGCAGACCCAGATGGCAGTGCTACAGAAAAACTTGGGCACAAGTCAGAAGACAAGCCTGACG
  ATCCCCAGCCAAAAATGGACTACGCTGGGAACGTGGCAGAGGCTGAGGGCCTCTTGGTGCCCCTGAGCAG
  CCCAGGAGACGGGCTCAAGCTTCCCGCATCTGACAGCGCCGAGGCCAGCAACAGCAGGGCCGACTGCTCC
  TGGACTCCACTCAACACCCAAATGAGCAAACAGGTTGACTGCTCACCCGCCGGAGTAAAGGCTTTGGACT
  CTCGGCAAGGTGTTGGAGAGAAGAATACTTTCATTTTGGCAACTCTGGGAACTGGAGTCCCTGTGGAGGG
  GACCCTGCCCCTGGTTACCACTAACTTCAGTCCTCTGCCAGCCCCTATCTGTCCCCCTGCTCCCGGTTCG
  GCCTCTGTGCCCCACTCTGTTCCAGATGCATTCCAGGTTCCCCTCTCCGTCCCTGCCCCAGTCCCCCATT
  CAGGGCTTGTTCCAGTCCAAGTTGCCACTTCGGTTCCAGCTCCTTCCCCTCCCTTAGCACCTGTCCCGGC
  TCTGGCTCCAGCGCCACCGTCAGTGCCCACGCTCATCTCTGACTCGAACCCCCTTTCTGTTTCGGCCTCA
  GTCTTGGTGCCTGTGCCAGCTTCTGCTCCCCCTTCAGGCCCGGTTCCCTTGTCGGCTCCAGCTCCTGCCC
  CGCTTTCAGTCCCAGTTTCAGCTCCTCCCTTGGCTCTCATCCAGGCTCCTGTGCCCCCTTCAGCTCCGAC
  CTTGGTTCTCGCTCCCGTCCCCACTCCGGTTCTGGCTCCCATGCCAGCATCCACGCCTCCAGCGGCCCCT
  GCCCCTCCGTCTGTGCCCATGCCCACTCCAACCCCATCTTCCGGCCCACCTTCTACCCCCACCCTCATCC
  CCGCCTTTGCTCCTACACCGGTGCCTGCACCCACCCCAGCCCCCATCTTTACTCCAGCCCCTACACCCAT
  GCCTGCTGCCACGCCAGCTGCCATTCCCACCTCTGCACCCATCCCGGCCTCCTTCAGTTTGAGTAGAGTG
  TGCTTTCCTGCAGCTCAGGCACCAGCTATGCAAAAAGTCCCCCTGTCCTTTCAGCCAGGGACAGTGCTGA
  CCCCGAGCCAGCCGCTGGTATATATCCCGCCTCCAAGCTGTGGGCAGCCACTCAGTGTGGCCACACTGCC
  AACCACTCTAGGGGTTTCCTCCACTCTTACGCTCCCTGTCCTGCCGTCCTACCTGCAGGACAGGTGTCTC
  CCAGGCGTGCTAGCCTCCCCCGAGCTCCGTTCTTACCCGTATGCATTTTCTGTGGCCCGGCCTCTGACTT
  CGGATTCCAAGCTGGTATCTCTGGAGGTGAACAGGCTCCCCTGCACTTCCCCATCCGGTAGCACCACCAC
  CCAGCCTGCACCCGATGGGGTCCCTGGGCCTTTGGCAGATACCTCCCTTGTTACTGCTTCTGCCAAGGTG
  CTTCCAACTCCACAGCCTCTGCTGCCAGCCCCCAGTGGGAGCTCAGCCCCACCGCACCCCGCCAAGATGC
  CCAGTGGCACCGAGCAGCAAACAGAAGGGACTTCCGTTACCTTCTCTCCTCTTAAGTCACCGCCACAGCT
  GGAACGAGAGATGGCCTCTCCACCTGAGTGCAGCGAGATGCCCCTTGATCTGTCCTCCAAGTCCAACCGC
  CAGAAGCTTCCATTGCCGAACCAGCGCAAGACACCCCCCATGCCTGTGTTGACCCCCGTGCACACCAGCA
  GCAAGGCCCTCCTCTCCACAGTCCTGTCTAGGTCTCAGCGCACAACCCAGGCTGCCGGTGGCAATGTCAC
  CTCCTGCCTGGGCTCCACTTCCTCGCCCTTTGTCATCTTTCCCGAGATCGTGAGGAATGGGGACCCGAGC
  ACCTGGGTGAAGAACTCAACTGCACTGATCAGCACCATTCCTGGCACCTACGTGGGAGTGGCCAACCCAG
  TGCCTGCATCCCTGCTGCTGAACAAAGACCCCAACCTGGGCCTCAACCGTGACCCCCGCCATCTCCCCAA
  GCAGGAGCCCATCTCCATCATTGATCAAGGAGAGCCTAAGGGCACTGGTGCCACGTGTGGCAAAAAGGGC
  AGCCAGGCTGGTGCTGAGGGACAGCCAAGCACAGTGAAACGATATACTCCAGCCCGCATTGCCCCTGGGC
  TGCCAGGGTGCCAAACCAAGGAACTCTCTTTGTGGAAACCCACGGGGCCGGCAAATATTTATCCCCGGTG
  TTCAGTCAATGGGAAACCTACCAGCACCCAGGTCCTGCCTGTTGGCTGGTCCCCGTACCACCAGGCGTCT
  CTGCTTTCCATTGGCATTTCCAGTGCCGGGCAGCTGACCCCCAGTCAGGGGGCGCCCATCAGGCCCACCA
  GCGTTGTTTCGGAGTTTTCTGGTGTGCCATCTCTCAGCTCCAGCGAAGCCGTGCACGGACTTCCTGAGGG
  GCAACCACGGCCTGGGGGCTCCTTCGTTCCAGAGCAGGACCCTGTTACAAAGAACAAAACTTGCCGGATT
  GCTGCCAAGCCTTATGAAGAACAAGTCAATCCTGTCCTCTTGACCCTCAGCCCTCAGACTGGGACCCTGG
  CACTGTCTGTTCAGCCTAGCGGTGGGGACATTCGAATGAATCAGGGGCCTGAGGAATCAGAGAGCCACCT
  CTGCTCTGACAGCACTCCTAAGATGGAAGGCCCCCAGGGGGCTTGTGGCCTGAAGCTGGCAGGAGACACG
  AAGCCTAAGAACCAAGTGCTGGCCACCTACATGTCCCATGAGCTGGTCCTGGCCACCCCCCAGAACCTGC
  CTAAGATGCCTGAGCTGCCTTTGCTACCTCACGACAGCCACCCCAAGGAACTTATATTGGACGTGGTTCC
  GAGCAGCAGGAGGGGCTCCAGCACAGAGCGCCCACAGCTTGGAAGCCAGGTGGATCTGGGGCGAGTGAAA
  ATGGAGAAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGATGGCCTCCCAGTGG
  CTCCCCAGAGGGGCCAAGCTGAAGTTCGGGCTAAGGCCGGGCAGGCTCGAGTGAAACAGGAAAGCGTAGG
  GGTCTTTGCTTGCAAGAACAAGTGGCAGCCAGATGATGTGACGGAATCTCTGCCGCCCAAGAAGATGAAG
  TGCGGCAAAGAGAAGGACAGTGAAGAGCAGCAGCTCCAGCCACAAGCCAAGGCCGTGGTCCGGAGTTCCC
  ACAGACCCAAGTGCCGGAAGCTGCCCAGTGACCCCCAGGAATCCACCAAGAAAAGCCCCAGGGGGGCTTC
  AGATTCAGGAAAAGAGCACAATGGAGTCAGGGGAAAGCACAAGCACCGGAAGCCGACAAAGCCGGAGTCC
  CAGTCTCCAGGAAAACGAGCCGACAGCCACGAGGAAGGTTCCTTGGAAAAGAAAGCAAAGAGCAGTTTCC
  GTGACTTTATTCCTGTGGTTCTGAGCACCCGCACGCGCAGTCAGTCTGGAAGCATCTGTAGCTCCTTTGC
  TGGCATGGCAGACAGTGACATGGGAAGCCAGGAAGTCTTCCCCACAGAAGAAGAAGAGGAGGTAACCCCC
  ACCCCAGCTAAGCGTCGAAAGGTGAGAAAGACCCAACGGGACACCCAGTATCGCAGCCACCATGCCCAGG
  ACAAGTCTCTGCTGAGCCAGGGCCGAAGGCACCTGTGGCGAGCCCGAGAAATGCCCTGGAGGACAGAGGC
  TGCCCGGCAAATGTGGGACACCAATGAGGAGGAGGAGGAAGAAGAGGAGGAGGGCCTGCTGAAGAGGAAG
  AAACGAAGACGGCAGAAGAGCCGAAAATATCAGACTGGGGAGTACCTGACAGAGCAAGAAGACGAGCAGC
  GGCGGAAAGGGAGAGCAGATTTAAAGGCCCGTAAGCAGAAGACTTCCTCCTCCCAAAGTTTGGAGCACCG
  CCTCAGGAACAGGAACCTTCTCTTGCCCAACAAAGTCCAGGGGATCTCGGATTCACCAAACGGTTTCCTC
  CCAAATAACCTGGAAGAGCCAGCCTGCCTTGAAAATTCAGAAAAGCCATCAGGAAAACGAAAGTGCAAGA
  CCAAGCACATGGCAACCGTCTCAGAAGAGGCAAAGGATGTTGTTCTCTACTGCCTCCAGAAAGACAGTGA
  AGATGTGAATCACCGTGACAATGCTGGCTACACAGCCCTGCATGAGGCTTGTTCCCGGGGCTGGACCGAC
  ATCCTGAACATCCTGCTGGAGCACGGGGCCAACGTGAACTGCAGTGCGCAGGACGGCACGAGGCCAGTTC
  ATGATGCGGTGGTCAATGACAACCTGGAGACCATCTGGCTCCTGCTGTCCTATGGGGCCGATCCCACACT
  GGCTACCTACTCGGGTCAGACAGCCATGAAGCTGGCCAGCAGCGACACCATGAAGCGCTTTCTCAGTGAT
  CACCTCTCGGATCTTCAGGGCCGGGCAGAGGGTGATCCCGGTGTATCCTGGGATTTTTACAGCAGTTCTG
  TGTTGGAGGAAAAAGACGGGTTTGCCTGTGACCTCCTACATAATCCTCCTGGGAGCTCAGATCAAGAAGG
  AGACGATCCGATGGAGGAGGATGATTTCATGTTTGAACTCTCAGACAAGCCTCTTCTCCCTTGCTACAAC
  CTCCAAGTGTCAGTGTCCCGCGGGCCCTGCAACTGGTTCCTCTTTTCCGATGTCTTGAAGAGGCTGAAGC
  TTTCCTCGAGGATCTTTCAGGCCCGGTTCCCGCACTTTGAAATCACCACCATGCCCAAGGCCGAGTTCTA
  CAGGCAGGTGGCCTCCAGTCAGCTGCTGACCCCTGCCGAGAGGCCTGGAGGCTTGGACGACAGATCCCCC
  CCAGGCTCCTCTGAGACTGTGGAGCTGGTGCGGTACGAGCCAGACCTACTTCGGCTCCTAGGGTCCGAGG
  TGGAATTCCAGTCTTGCAACAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219297 representing NM_021946
  Red=Cloning site Green=Tags(s)
  
  MISTAPLYSGVHNWTSSDRIRMCGINEERRAPLSDEESTTGDCQHFGSQEFCVSSSFSKVELTAVGSGSN
  ARGADPDGSATEKLGHKSEDKPDDPQPKMDYAGNVAEAEGLLVPLSSPGDGLKLPASDSAEASNSRADCS
  WTPLNTQMSKQVDCSPAGVKALDSRQGVGEKNTFILATLGTGVPVEGTLPLVTTNFSPLPAPICPPAPGS
  ASVPHSVPDAFQVPLSVPAPVPHSGLVPVQVATSVPAPSPPLAPVPALAPAPPSVPTLISDSNPLSVSAS
  VLVPVPASAPPSGPVPLSAPAPAPLSVPVSAPPLALIQAPVPPSAPTLVLAPVPTPVLAPMPASTPPAAP
  APPSVPMPTPTPSSGPPSTPTLIPAFAPTPVPAPTPAPIFTPAPTPMPAATPAAIPTSAPIPASFSLSRV
  CFPAAQAPAMQKVPLSFQPGTVLTPSQPLVYIPPPSCGQPLSVATLPTTLGVSSTLTLPVLPSYLQDRCL
  PGVLASPELRSYPYAFSVARPLTSDSKLVSLEVNRLPCTSPSGSTTTQPAPDGVPGPLADTSLVTASAKV
  LPTPQPLLPAPSGSSAPPHPAKMPSGTEQQTEGTSVTFSPLKSPPQLEREMASPPECSEMPLDLSSKSNR
  QKLPLPNQRKTPPMPVLTPVHTSSKALLSTVLSRSQRTTQAAGGNVTSCLGSTSSPFVIFPEIVRNGDPS
  TWVKNSTALISTIPGTYVGVANPVPASLLLNKDPNLGLNRDPRHLPKQEPISIIDQGEPKGTGATCGKKG
  SQAGAEGQPSTVKRYTPARIAPGLPGCQTKELSLWKPTGPANIYPRCSVNGKPTSTQVLPVGWSPYHQAS
  LLSIGISSAGQLTPSQGAPIRPTSVVSEFSGVPSLSSSEAVHGLPEGQPRPGGSFVPEQDPVTKNKTCRI
  AAKPYEEQVNPVLLTLSPQTGTLALSVQPSGGDIRMNQGPEESESHLCSDSTPKMEGPQGACGLKLAGDT
  KPKNQVLATYMSHELVLATPQNLPKMPELPLLPHDSHPKELILDVVPSSRRGSSTERPQLGSQVDLGRVK
  MEKVDGDVVFNLATCFRADGLPVAPQRGQAEVRAKAGQARVKQESVGVFACKNKWQPDDVTESLPPKKMK
  CGKEKDSEEQQLQPQAKAVVRSSHRPKCRKLPSDPQESTKKSPRGASDSGKEHNGVRGKHKHRKPTKPES
  QSPGKRADSHEEGSLEKKAKSSFRDFIPVVLSTRTRSQSGSICSSFAGMADSDMGSQEVFPTEEEEEVTP
  TPAKRRKVRKTQRDTQYRSHHAQDKSLLSQGRRHLWRAREMPWRTEAARQMWDTNEEEEEEEEEGLLKRK
  KRRRQKSRKYQTGEYLTEQEDEQRRKGRADLKARKQKTSSSQSLEHRLRNRNLLLPNKVQGISDSPNGFL
  PNNLEEPACLENSEKPSGKRKCKTKHMATVSEEAKDVVLYCLQKDSEDVNHRDNAGYTALHEACSRGWTD
  ILNILLEHGANVNCSAQDGTRPVHDAVVNDNLETIWLLLSYGADPTLATYSGQTAMKLASSDTMKRFLSD
  HLSDLQGRAEGDPGVSWDFYSSSVLEEKDGFACDLLHNPPGSSDQEGDDPMEEDDFMFELSDKPLLPCYN
  LQVSVSRGPCNWFLFSDVLKRLKLSSRIFQARFPHFEITTMPKAEFYRQVASSQLLTPAERPGGLDDRSP
  PGSSETVELVRYEPDLLRLLGSEVEFQSCNS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021946
• ORF Size: 5133 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021946.5
• RefSeq Size: 7127 bp
• RefSeq ORF: 5136 bp
• Locus ID: 63035
• UniProt ID: Q5H9F3
• Cytogenetics: Xq26.1
• MW: 182.5 kDa
• Gene Summary: The protein encoded by this gene is a transcriptional corepressor that is found tethered to promoter regions by DNA-binding proteins. The encoded protein can interact with several different class II histone deacetylases to repress transcription. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010]