ASPHD1 (NM_181718) Human Tagged ORF Clone

CAT#: RC219226

ASPHD1 (Myc-DDK-tagged)-Human aspartate beta-hydroxylase domain containing 1 (ASPHD1)

Product Images

Circular map for RC219226

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  Western blot validation of overexpression lysate (Cat# LY405633) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219226 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ASPHD1 protein (Cat# TP319226). The protein was produced from HEK293T cells transfected with ASPHD1 cDNA clone (Cat# RC219226) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ASPHD1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219226 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGAGGGGAGAGGGAGCTTCAGCGTGGAGAGAGGACCGCGGAAGGAGAGAGAGACAGCCCAGAGTG
  GAATGTGGAAGGGAAACAGTCCAGCGGGGAGCCAGGGGGCAGCCATGGAAGGGACAGGTGGGGAGCTGGG
  GGGACAGGGGAACTGGGGTCCGGAGGACGCCCCAGGCCTCTTGGCCAGGGCCTCCCTGATCATGCTCCCG
  TGGCCACTACCCCTGGCCTCCTCGGCCCTCACCTTGCTCTTCGGGGCCCTCACTTCCCTGTTCCTCTGGT
  ACTGCTACCGCCTGGGCTCCCAAGACATGCAGGCCCTAGGGGCTGGGAGCCGAGCTGGGGGTGTTCGTGG
  TGGGCCTGTGGGATGCTCGGAGGCCGGCGGGCCAAGCCCAGGGGGTCCTGGGGATCCCGGGGAAGGACCT
  AGGACGGAAGGCCTAGTGAGCCGGCGGCTTCGGGCCTACGCAAGGCGCTACTCCTGGGCTGGGATGGGTA
  GAGTGAGGCGGGCAGCTCAGGGTGGCCCAGGCCCTGGGAGAGGGCCAGGGGTCCTAGGTATTCAGCGCCC
  AGGCCTGCTTTTCCTACCAGACCTGCCTTCAGCCCCCTTTGTGCCGCGGGACGCCCAGCGGCACGACGTG
  GAGCTCCTGGAGAGCAGCTTCCCTGCCATTTTGCGGGACTTCGGGGCTGTGAGCTGGGACTTCTCAGGGA
  CTACCCCTCCGCCTCGGGGCTGGTCCCCACCTCTGGCCCCCGGGTGCTACCAGCTCCTGCTGTACCAAGC
  AGGCCGGTGCCAACCCAGCAACTGCCGCCGGTGCCCGGGGGCCTATCGGGCACTGAGGGGGCTTCGAAGC
  TTTATGAGTGCCAACACCTTCGGCAATGCCGGCTTTTCCGTTCTCCTGCCTGGGGCCCGGCTCGAGGGCC
  GCTGTGGGCCCACCAATGCCCGGGTCAGATGCCATCTGGGCCTAAAGATCCCTCCTGGCTGTGAGCTGGT
  GGTCGGCGGTGAGCCCCAGTGCTGGGCTGAGGGGCACTGTCTACTGGTGGACGACTCTTTTCTACACACA
  GTGGCTCACAATGGCTCCCCCGAAGATGGGCCTCGAGTGGTCTTCATCGTGGACCTCTGGCACCCCAACG
  TGGCAGGGGCTGAGCGCCAGGCCCTCGACTTTGTCTTCGCCCCAGACCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219226 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKEGRGSFSVERGPRKERETAQSGMWKGNSPAGSQGAAMEGTGGELGGQGNWGPEDAPGLLARASLIMLP
  WPLPLASSALTLLFGALTSLFLWYCYRLGSQDMQALGAGSRAGGVRGGPVGCSEAGGPSPGGPGDPGEGP
  RTEGLVSRRLRAYARRYSWAGMGRVRRAAQGGPGPGRGPGVLGIQRPGLLFLPDLPSAPFVPRDAQRHDV
  ELLESSFPAILRDFGAVSWDFSGTTPPPRGWSPPLAPGCYQLLLYQAGRCQPSNCRRCPGAYRALRGLRS
  FMSANTFGNAGFSVLLPGARLEGRCGPTNARVRCHLGLKIPPGCELVVGGEPQCWAEGHCLLVDDSFLHT
  VAHNGSPEDGPRVVFIVDLWHPNVAGAERQALDFVFAPDP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181718
• ORF Size: 1170 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181718.4
• RefSeq Size: 1562 bp
• RefSeq ORF: 1173 bp
• Locus ID: 253982
• UniProt ID: Q5U4P2
• Cytogenetics: 16p11.2
• MW: 41.1 kDa