PAG (PAG1) (NM_018440) Human Tagged ORF Clone

CAT#: RC219183

PAG1 (Myc-DDK-tagged)-Human phosphoprotein associated with glycosphingolipid microdomains 1 (PAG1)

Product Images

Circular map for RC219183

• 

  Western blot validation of overexpression lysate (Cat# LY402686) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219183 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PAG
• Synonyms: CBP; PAG
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219183 representing NM_018440
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCCCGCGGGGAGCCTGCTGGGCAGCGGACAGATGCAGATCACCCTGTGGGGAAGTCTGGCCGCTG
  TCGCCATTTTCTTCGTCATCACCTTCCTCATCTTCCTGTGCTCTAGTTGTGACAGGGAAAAGAAGCCGCG
  ACAGCATAGTGGGGACCATGAGAACCTGATGAACGTGCCTTCAGACAAGGAGATGTTCAGCCGTTCAGTT
  ACTAGCCTGGCAACAGATGCTCCTGCCAGCAGTGAGCAGAATGGGGCACTCACCAATGGGGACATTCTTT
  CAGAGGACAGTACTCTGACCTGCATGCAGCATTACGAGGAAGTCCAGACATCGGCCTCGGATCTGCTGGA
  TTCCCAGGACAGCACAGGGAAACCAAAATGTCATCAGAGTCGGGAGCTGCCCAGAATCCCTCCCGAGAGC
  GCAGTGGATACCATGCTCACGGCGAGAAGTGTGGACGGGGACCAGGGGCTGGGGATGGAAGGGCCCTATG
  AAGTGCTCAAGGACAGCTCCTCCCAAGAAAACATGGTGGAGGACTGCTTGTATGAAACTGTGAAAGAGAT
  CAAGGAGGTGGCTGCAGCTGCACACCTGGAGAAAGGCCACAGTGGCAAGGCAAAATCTACTTCTGCCTCG
  AAAGAGCTCCCAGGGCCCCAGACTGAAGGCAAAGCTGAGTTTGCTGAATATGCCTCGGTGGACAGAAACA
  AAAAATGTCGTCAAAGTGTTAATGTAGAGAGTATCCTTGGAAATTCATGTGATCCAGAAGAGGAGGCCCC
  ACCACCTGTCCCTGTTAAGCTTCTGGACGAGAATGAAAACCTTCAGGAGAAGGAAGGGGGAGAGGCGGAA
  GAGAGTGCCACAGACACGACCAGTGAAACTAACAAGAGATTTAGCTCATTGTCATACAAGTCTCGGGAAG
  AAGACCCCACTCTCACAGAAGAAGAGATCTCAGCTATGTACTCATCAGTAAATAAACCTGGACAGTTAGT
  GAATAAATCGGGGCAGTCGCTTACAGTTCCGGAGTCCACCTACACCTCCATTCAAGGGGACCCACAGAGG
  TCACCCTCCTCCTGTAATGATCTCTATGCTACTGTTAAAGACTTCGAAAAAACTCCAAACAGCACACTTC
  CACCAGCAGGGAGGCCCAGCGAGGAGCCAGAGCCTGATTATGAAGCGATACAGACTCTCAACAGAGAGGA
  AGAAAAGGCCACCCTGGGGACCAATGGCCACCACGGTCTCGTCCCAAAGGAGAACGACTACGAGAGCATA
  AGTGACTTGCAGCAAGGCAGAGATATTACCAGGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219183 representing NM_018440
  Red=Cloning site Green=Tags(s)
  
  MGPAGSLLGSGQMQITLWGSLAAVAIFFVITFLIFLCSSCDREKKPRQHSGDHENLMNVPSDKEMFSRSV
  TSLATDAPASSEQNGALTNGDILSEDSTLTCMQHYEEVQTSASDLLDSQDSTGKPKCHQSRELPRIPPES
  AVDTMLTARSVDGDQGLGMEGPYEVLKDSSSQENMVEDCLYETVKEIKEVAAAAHLEKGHSGKAKSTSAS
  KELPGPQTEGKAEFAEYASVDRNKKCRQSVNVESILGNSCDPEEEAPPPVPVKLLDENENLQEKEGGEAE
  ESATDTTSETNKRFSSLSYKSREEDPTLTEEEISAMYSSVNKPGQLVNKSGQSLTVPESTYTSIQGDPQR
  SPSSCNDLYATVKDFEKTPNSTLPPAGRPSEEPEPDYEAIQTLNREEEKATLGTNGHHGLVPKENDYESI
  SDLQQGRDITRL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018440
• ORF Size: 1296 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018440.4
• RefSeq Size: 10759 bp
• RefSeq ORF: 1299 bp
• Locus ID: 55824
• UniProt ID: Q9NWQ8
• Cytogenetics: 8q21.13
• Protein Families: Druggable Genome, Transmembrane
• MW: 46.8 kDa
• Gene Summary: The protein encoded by this gene is a type III transmembrane adaptor protein that binds to the tyrosine kinase csk protein. It is thought to be involved in the regulation of T cell activation. [provided by RefSeq, Jul 2008]