UBXN2B (NM_001077619) Human Tagged ORF Clone

CAT#: RC219137

UBXN2B (Myc-DDK-tagged)-Human UBX domain protein 2B (UBXN2B)

Product Images

Circular map for RC219137

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY UBXN2B (Cat# RC219137, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBXN2B(Cat# TA502631). Positive lysates LY421462 (100ug) and LC421462 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY421462) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219137 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified UBXN2B protein (Cat# TP319137). The protein was produced from HEK293T cells transfected with UBXN2B cDNA clone (Cat# RC219137) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UBXN2B
• Synonyms: p37
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219137 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGGGCGGAGGCCCTGAGCCCGGCGAGCAGGAGAGGAGGTCTTCCGGGCCGCGGCCTCCGAGCG
  CGCGGGATTTGCAGTTGGCCTTGGCAGAATTGTATGAAGATGAGGTGAAGTGCAAATCTTCCAAGTCTAA
  TAGACCTAAAGCCACAGTCTTCAAGAGCCCACGGACACCACCTCAACGGTTTTACTCAAGTGAACATGAA
  TACAGTGGATTAAATATAGTTCGACCTTCAACTGGGAAAATTGTGAATGAACTTTTCAAAGAGGCAAGGG
  AACATGGGGCTGTCCCTCTGAATGAAGCCACAAGAGCTTCAGGTGATGATAAATCTAAGTCATTTACAGG
  TGGAGGATACAGATTGGGTAGTTCTTTTTGTAAGCGGTCTGAATATATCTATGGAGAAAATCAGCTGCAA
  GATGTTCAGATTTTGCTTAAACTGTGGAGCAATGGTTTCAGTTTAGATGATGGAGAATTGAGACCTTACA
  ATGAACCAACAAATGCTCAATTTCTGGAGTCTGTTAAGAGAGGAGAGATTCCCCTGGAGCTTCAGCGCCT
  TGTTCATGGTGGCCAAGTGAATTTGGATATGGAGGATCATCAGGATCAAGAATACATAAAACCTAGATTG
  AGGTTCAAGGCTTTTAGTGGAGAAGGGCAAAAACTTGGAAGCCTTACACCTGAAATAGTCAGTACACCTT
  CCTCTCCAGAAGAGGAGGATAAATCAATACTTAATGCAGTTGTTCTTATTGATGATTCAGTGCCAACAAC
  AAAAATTCAAATCAGGTTAGCAGATGGGAGTCGTTTGATACAAAGATTCAATAGTACACACAGGATCCTG
  GATGTCCGGAACTTTATTGTACAGTCTCGTCCTGAATTTGCGGCTCTTGACTTTATTCTTGTGACTTCAT
  TTCCGAATAAAGAGCTAACAGATGAAAGCCTGACACTGCTAGAAGCAGATATTCTTAACACTGTGTTACT
  CCAGCAACTAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219137 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAEGGGPEPGEQERRSSGPRPPSARDLQLALAELYEDEVKCKSSKSNRPKATVFKSPRTPPQRFYSSEHE
  YSGLNIVRPSTGKIVNELFKEAREHGAVPLNEATRASGDDKSKSFTGGGYRLGSSFCKRSEYIYGENQLQ
  DVQILLKLWSNGFSLDDGELRPYNEPTNAQFLESVKRGEIPLELQRLVHGGQVNLDMEDHQDQEYIKPRL
  RFKAFSGEGQKLGSLTPEIVSTPSSPEEEDKSILNAVVLIDDSVPTTKIQIRLADGSRLIQRFNSTHRIL
  DVRNFIVQSRPEFAALDFILVTSFPNKELTDESLTLLEADILNTVLLQQLK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001077619
• ORF Size: 993 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001077619.1, NP_001071087.1
• RefSeq Size: 5088 bp
• RefSeq ORF: 996 bp
• Locus ID: 137886
• UniProt ID: Q14CS0
• Cytogenetics: 8q12.1
• MW: 37.1 kDa
• Gene Summary: Adapter protein required for Golgi and endoplasmic reticulum biogenesis (PubMed:17141156). Involved in Golgi and endoplasmic reticulum maintenance during interphase and in their reassembly at the end of mitosis (PubMed:17141156). The complex formed with VCP has membrane fusion activity; membrane fusion activity requires USO1-GOLGA2 tethering and BET1L (PubMed:17141156). VCPIP1 is also required, but not its deubiquitinating activity (PubMed:17141156). Together with NSFL1C/p47, regulates the centrosomal levels of kinase AURKA/Aurora A during mitotic progression by promoting AURKA removal from centrosomes in prophase (PubMed:23649807). Also, regulates spindle orientation during mitosis (PubMed:23649807).[UniProtKB/Swiss-Prot Function]