IGFBPL1 (NM_001007563) Human Tagged ORF Clone

CAT#: RC219122

IGFBPL1 (Myc-DDK-tagged)-Human insulin-like growth factor binding protein-like 1 (IGFBPL1)

Product Images

Circular map for RC219122

• 

  Western blot validation of overexpression lysate (Cat# LY423513) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219122 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified IGFBPL1 protein (Cat# TP319122). The protein was produced from HEK293T cells transfected with IGFBPL1 cDNA clone (Cat# RC219122) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IGFBPL1
• Synonyms: bA113O24.1; IGFBP-RP4; IGFBPRP4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219122 representing NM_001007563
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCGCTTGTCTCTGCTCTTGCCGCTGCTGCTTCTGCTGCTGCTGCCGCTGCTGCCGCCGCTGTCCC
  CGAGCCTTGGGATCCGCGACGTGGGCGGCCGGCGCCCCAAGTGTGGTCCGTGCCGGCCAGAGGGCTGCCC
  GGCGCCTGCGCCCTGCCCGGCGCCCGGGATCTCGGCGCTCGACGAGTGCGGCTGCTGCGCCCGCTGCCTG
  GGAGCCGAGGGCGCGAGCTGCGGGGGCCGCGCCGGCGGGCGCTGTGGCCCCGGCCTGGTATGCGCGAGCC
  AGGCCGCTGGGGCAGCGCCCGAGGGCACCGGGCTCTGCGTGTGCGCGCAGCGCGGCACCGTCTGCGGCTC
  CGACGGTCGCTCGTACCCCAGCGTCTGCGCGCTGCGCCTGCGCGCTCGGCACACGCCCCGCGCGCACCCC
  GGTCACCTGCACAAGGCGCGCGACGGCCCTTGCGAGTTCGCTCCTGTGGTCGTCGTTCCTCCCCGAAGTG
  TTCACAACGTCACCGGGGCGCAGGTGGGCCTGTCCTGTGAAGTGAGGGCTGTGCCTACCCCAGTCATCAC
  GTGGAGAAAGGTCACGAAGTCCCCTGAGGGCACCCAAGCACTGGAGGAGCTGCCTGGGGACCATGTCAAT
  ATAGCTGTCCAAGTGCGAGGGGGCCCTTCTGACCATGAGGCCACGGCCTGGATTTTGATCAACCCCCTGC
  GAAAGGAGGATGAGGGTGTGTACCAGTGCCATGCAGCCAACATGGTGGGAGAGGCTGAGTCCCACAGCAC
  AGTGACGGTTCTAGATCTGAGTAAATACAGGAGCTTCCACTTCCCAGCTCCCGATGACCGCATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219122 representing NM_001007563
  Red=Cloning site Green=Tags(s)
  
  MPRLSLLLPLLLLLLLPLLPPLSPSLGIRDVGGRRPKCGPCRPEGCPAPAPCPAPGISALDECGCCARCL
  GAEGASCGGRAGGRCGPGLVCASQAAGAAPEGTGLCVCAQRGTVCGSDGRSYPSVCALRLRARHTPRAHP
  GHLHKARDGPCEFAPVVVVPPRSVHNVTGAQVGLSCEVRAVPTPVITWRKVTKSPEGTQALEELPGDHVN
  IAVQVRGGPSDHEATAWILINPLRKEDEGVYQCHAANMVGEAESHSTVTVLDLSKYRSFHFPAPDDRM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001007563
• ORF Size: 834 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001007563.3
• RefSeq Size: 1093 bp
• RefSeq ORF: 837 bp
• Locus ID: 347252
• UniProt ID: Q8WX77
• Cytogenetics: 9p13.1
• MW: 28.8 kDa
• Gene Summary: IGF-binding proteins prolong the half-life of IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs in cell culture. They alter the interaction of IGFs with their cell surface receptors (By similarity). May be a putative tumor suppressor protein.[UniProtKB/Swiss-Prot Function]