PTP gamma (PTPRG) (NM_002841) Human Tagged ORF Clone

CAT#: RC218964

PTPRG (Myc-DDK-tagged)-Human protein tyrosine phosphatase, receptor type, G (PTPRG)

Product Images

Circular map for RC218964

• 

  Western blot validation of overexpression lysate (Cat# LY419069) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218964 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PTP gamma
• Synonyms: HPTPG; PTPG; R-PTP-GAMMA; RPTPG
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218964 representing NM_002841
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGAGGTTACTGGAACCGTGTTGGTGGATTTTGTTCCTGAAAATCACCAGTTCCGTGCTCCATTATG
  TCGTGTGCTTCCCCGCGTTGACAGAAGGCTACGTTGGGGCCCTGCACGAGAATAGACACGGCAGCGCAGT
  GCAGATCCGCAGGCGCAAGGCTTCAGGCGACCCGTACTGGGCCTACTCTGGTGCCTATGGTCCTGAGCAC
  TGGGTCACGTCTAGTGTCAGCTGTGGGAGCCGTCACCAGTCTCCTATTGACATTTTAGACCAGTATGCGC
  GTGTTGGGGAAGAATACCAGGAACTGCAACTCGATGGCTTCGACAATGAGTCTTCTAACAAAACCTGGAT
  GAAAAACACAGGGAAAACAGTCGCCATCCTTCTGAAAGACGACTATTTTGTCAGTGGAGCTGGTCTACCT
  GGCAGATTCAAAGCTGAGAAGGTGGAATTTCACTGGGGCCACAGCAATGGCTCAGCGGGCTCTGAACACA
  GCATCAATGGCAGGAGGTTTCCTGTTGAGATGCAGATTTTCTTTTACAATCCAGATGACTTTGACAGCTT
  TCAAACCGCAATTTCTGAGAACAGAATAATCGGAGCCATGGCCATATTTTTTCAAGTCAGTCCGAGGGAC
  AATTCTGCACTGGATCCTATTATCCACGGGTTGAAGGGTGTCGTACATCATGAGAAGGAGACCTTTCTGG
  ATCCTTTCGTCCTCCGGGACCTCCTGCCTGCATCCCTGGGCAGCTATTATCGGTACACAGGTTCCTTGAC
  CACACCACCGTGTAGCGAAATAGTGGAGTGGATAGTCTTCCGGAGACCCGTCCCCATCTCTTACCATCAG
  CTTGAGGCTTTTTATTCCATCTTCACCACGGAGCAGCAAGACCATGTCAAGTCGGTGGAGTATCTGAGAA
  ATAACTTTCGACCACAGCAGCGTCTGCATGACAGGGTGGTGTCCAAGTCCGCCGTCCGTGACTCCTGGAA
  CCACGACATGACAGACTTCTTAGAAAACCCACTGGGGACAGAAGCCTCTAAAGTTTGCAGCTCTCCACCC
  ATCCACATGAAGGTGCAGCCTCTGAACCAGACGGCACTGCAGGTGTCCTGGAGCCAGCCGGAGACTATCT
  ACCACCCACCCATCATGAACTACATGATCTCCTACAGCTGGACCAAGAATGAGGACGAGAAGGAGAAGAC
  GTTTACAAAGGACAGCGACAAAGACTTGAAAGCCACCATTAGCCATGTCTCACCCGATAGCCTTTACCTG
  TTCCGAGTCCAGGCCGTGTGTCGGAACGACATGCGCAGCGACTTTAGCCAGACGATGCTGTTTCAAGCTA
  ATACCACTCGAATATTCCAAGGGACCAGAATAGTGAAAACAGGAGTGCCCACAGCGTCTCCTGCCTCTTC
  AGCCGACATGGCCCCCATCAGCTCGGGGTCTTCTACCTGGACGTCCTCTGGCATCCCATTCTCATTTGTT
  TCCATGGCAACTGGGATGGGCCCCTCCTCCAGTGGCAGCCAGGCCACAGTGGCCTCGGTGGTCACCAGCA
  CGCTGCTCGCCGGCCTGGGGTTCGGCGGTGGTGGCATCTCCTCTTTCCCCAGCACTGTGTGGCCCACGCG
  CCTCCCGACGGCCGCCTCAGCCAGCAAGCAGGCGGCTAGGCCAGTCCTAGCCACCACAGAGGCCTTGGCT
  TCTCCAGGGCCCGATGGTGATTCGTCACCAACCAAGGACGGCGAGGGCACCGAGGAAGGAGAGAAGGATG
  AGAAAAGCGAGAGTGAGGATGGGGAGCGGGAGCACGAGGAGGATGGAGAGAAGGACTCCGAAAAGAAGGA
  GAAGAGTGGGGTGACCCACGCTGCCGAGGAGCGGAATCAGACGGAGCCCAGCCCCACACCCTCGTCTCCT
  AACAGGACTGCCGAGGGAGGGCATCAGACTATACCTGGGCATGAGCAGGATCACACTGCCGTCCCCACAG
  ACCAGACGGGCGGAAGGAGGGATGCCGGCCCAGGCCTGGACCCCGACATGGTCACCTCCACCCAAGTGCC
  CCCCACCGCCACAGAGGAGCAGTATGCAGGGAGTGATCCCAAGAGGCCCGAAATGCCATCTAAAAAGCCT
  ATGTCCCGCGGGGACCGATTTTCTGAAGACAGCAGATTTATCACTGTTAATCCAGCGGAAAAAAACACCT
  CTGGAATGATAAGCCGCCCTGCTCCAGGGAGGATGGAGTGGATCATCCCTCTGATTGTGGTATCAGCCTT
  GACCTTCGTGTGCCTCATCCTTCTCATTGCTGTGCTCGTTTACTGGAGAGGGTGTAACAAAATAAAGTCC
  AAGGGCTTTCCCAGACGTTTCCGTGAAGTGCCTTCTTCTGGGGAGAGAGGAGAGAAGGGGAGCAGAAAAT
  GTTTTCAGACTGCTCATTTCTATGTGGAAGACAGCAGTTCACCTCGAGTGGTCCCTAATGAAAGTATTCC
  TATTATTCCTATTCCGGATGACATGGAAGCCATTCCTGTCAAACAGTTTGTCAAACACATCGGTGAGCTC
  TATTCTAATAACCAGCATGGGTTCTCTGAGGATTTTGAGGAAGTCCAGCGCTGTACTGCTGATATGAACA
  TCACTGCAGAGCATTCCAATCATCCAGAAAACAAGCACAAAAACAGATACATCAACATTTTAGCATATGA
  TCACAGTAGGGTGAAGTTAAGACCTTTACCAGGAAAAGACTCTAAGCACAGCGACTACATTAATGCAAAC
  TATGTTGATGGTTACAACAAAGCAAAAGCCTACATTGCCACCCAAGGACCTTTGAAGTCTACATTTGAAG
  ATTTCTGGAGGATGATTTGGGAACAAAACACTGGAATCATTGTGATGATTACGAACCTTGTGGAAAAAGG
  AAGACGAAAATGTGATCAGTATTGGCCAACAGAGAACAGTGAGGAATATGGAAACATTATTGTCACGCTG
  AAGAGCACAAAAATACATGCCTGCTACACTGTTCGTCGTTTTTCAATCAGAAATACAAAAGTGAAAAAGG
  GTCAGAAGGGAAATCCCAAGGGTCGTCAGAATGAAAGGGTAGTGATCCAGTATCACTATACACAGTGGCC
  TGACATGGGAGTTCCCGAGTATGCCCTTCCAGTACTGACTTTCGTGAGGAGATCCTCAGCAGCTCGGATG
  CCAGAAACGGGCCCTGTGTTGGTGCACTGCAGTGCTGGTGTGGGCAGAACAGGCACCTATATTGTAATAG
  ACAGCATGCTGCAACAGATAAAAGACAAAAGCACAGTTAACGTCCTGGGATTCCTGAAGCATATCAGGAC
  ACAGCGTAACTACCTCGTCCAGACTGAGGAGCAGTACATTTTCATCCATGATGCCTTGTTGGAAGCCATT
  CTTGGAAAGGAGACTGAAGTATCTTCAAATCAGCTGCACAGCTATGTTAACAGCATCCTTATACCAGGAG
  TAGGAGGAAAGACACGACTGGAAAAGCAATTCAAGCTGGTCACACAGTGTAATGCAAAATATGTGGAATG
  TTTCAGTGCTCAGAAAGAGTGTAACAAAGAAAAGAACAGAAACTCTTCAGTTGTGCCATCTGAGCGTGCT
  CGAGTGGGTCTTGCACCATTGCCTGGAATGAAAGGAACAGATTACATTAATGCTTCTTATATCATGGGCT
  ATTATAGGAGCAATGAATTTATTATAACTCAGCATCCTCTGCCACATACTACGAAAGATTTCTGGCGAAT
  GATTTGGGATCATAACGCACAGATCATTGTCATGCTGCCAGACAACCAGAGCTTGGCAGAAGATGAGTTT
  GTGTACTGGCCAAGTCGAGAAGAATCCATGAACTGTGAGGCCTTTACCGTCACCCTTATCAGCAAAGACA
  GACTGTGCCTCTCTAATGAAGAACAAATTATCATCCATGACTTTATCCTTGAAGCTACACAGGATGACTA
  TGTCTTAGAAGTTCGGCACTTTCAGTGTCCCAAATGGCCTAACCCAGATGCCCCCATAAGTAGTACCTTT
  GAACTTATCAACGTCATCAAGGAAGAGGCCTTAACAAGGGATGGTCCCACCATTGTTCATGATGAGTATG
  GAGCAGTTTCAGCAGGAATGTTATGTGCCCTTACCACCCTGTCCCAGCAACTGGAGAATGAAAATGCTGT
  GGATGTTTTCCAGGTTGCAAAAATGATCAATCTTATGAGGCCTGGAGTATTCACAGACATTGAACAATAC
  CAGTTCATCTATAAAGCAAGGCTTAGCTTGGTCAGCACTAAAGAAAATGGAAATGGTCCCATGACAGTAG
  ACAAAAATGGTGCTGTTCTTATTGCAGATGAATCAGACCCTGCTGAGAGCATGGAGTCCCTAGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218964 representing NM_002841
  Red=Cloning site Green=Tags(s)
  
  MRRLLEPCWWILFLKITSSVLHYVVCFPALTEGYVGALHENRHGSAVQIRRRKASGDPYWAYSGAYGPEH
  WVTSSVSCGSRHQSPIDILDQYARVGEEYQELQLDGFDNESSNKTWMKNTGKTVAILLKDDYFVSGAGLP
  GRFKAEKVEFHWGHSNGSAGSEHSINGRRFPVEMQIFFYNPDDFDSFQTAISENRIIGAMAIFFQVSPRD
  NSALDPIIHGLKGVVHHEKETFLDPFVLRDLLPASLGSYYRYTGSLTTPPCSEIVEWIVFRRPVPISYHQ
  LEAFYSIFTTEQQDHVKSVEYLRNNFRPQQRLHDRVVSKSAVRDSWNHDMTDFLENPLGTEASKVCSSPP
  IHMKVQPLNQTALQVSWSQPETIYHPPIMNYMISYSWTKNEDEKEKTFTKDSDKDLKATISHVSPDSLYL
  FRVQAVCRNDMRSDFSQTMLFQANTTRIFQGTRIVKTGVPTASPASSADMAPISSGSSTWTSSGIPFSFV
  SMATGMGPSSSGSQATVASVVTSTLLAGLGFGGGGISSFPSTVWPTRLPTAASASKQAARPVLATTEALA
  SPGPDGDSSPTKDGEGTEEGEKDEKSESEDGEREHEEDGEKDSEKKEKSGVTHAAEERNQTEPSPTPSSP
  NRTAEGGHQTIPGHEQDHTAVPTDQTGGRRDAGPGLDPDMVTSTQVPPTATEEQYAGSDPKRPEMPSKKP
  MSRGDRFSEDSRFITVNPAEKNTSGMISRPAPGRMEWIIPLIVVSALTFVCLILLIAVLVYWRGCNKIKS
  KGFPRRFREVPSSGERGEKGSRKCFQTAHFYVEDSSSPRVVPNESIPIIPIPDDMEAIPVKQFVKHIGEL
  YSNNQHGFSEDFEEVQRCTADMNITAEHSNHPENKHKNRYINILAYDHSRVKLRPLPGKDSKHSDYINAN
  YVDGYNKAKAYIATQGPLKSTFEDFWRMIWEQNTGIIVMITNLVEKGRRKCDQYWPTENSEEYGNIIVTL
  KSTKIHACYTVRRFSIRNTKVKKGQKGNPKGRQNERVVIQYHYTQWPDMGVPEYALPVLTFVRRSSAARM
  PETGPVLVHCSAGVGRTGTYIVIDSMLQQIKDKSTVNVLGFLKHIRTQRNYLVQTEEQYIFIHDALLEAI
  LGKETEVSSNQLHSYVNSILIPGVGGKTRLEKQFKLVTQCNAKYVECFSAQKECNKEKNRNSSVVPSERA
  RVGLAPLPGMKGTDYINASYIMGYYRSNEFIITQHPLPHTTKDFWRMIWDHNAQIIVMLPDNQSLAEDEF
  VYWPSREESMNCEAFTVTLISKDRLCLSNEEQIIIHDFILEATQDDYVLEVRHFQCPKWPNPDAPISSTF
  ELINVIKEEALTRDGPTIVHDEYGAVSAGMLCALTTLSQQLENENAVDVFQVAKMINLMRPGVFTDIEQY
  QFIYKARLSLVSTKENGNGPMTVDKNGAVLIADESDPAESMESLV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002841
• ORF Size: 4335 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002841.2, NP_002832.2
• RefSeq Size: 5787 bp
• RefSeq ORF: 4338 bp
• Locus ID: 5793
• UniProt ID: P23470
• Cytogenetics: 3p14.2
• Domains: Y_phosphatase, carb_anhydrase, PTPc_motif, FN3
• Protein Families: Druggable Genome, Phosphatase
• MW: 161.8 kDa
• Gene Summary: The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. The extracellular region of this PTP contains a carbonic anhydrase-like (CAH) domain, which is also found in the extracellular region of PTPRBETA/ZETA. This gene is located in a chromosomal region that is frequently deleted in renal cell carcinoma and lung carcinoma, thus is thought to be a candidate tumor suppressor gene. [provided by RefSeq, Jul 2008]