PLEKHH2 (NM_172069) Human Tagged ORF Clone

CAT#: RC218833

PLEKHH2 (Myc-DDK-tagged)-Human pleckstrin homology domain containing, family H (with MyTH4 domain) member 2 (PLEKHH2)

Product Images

Circular map for RC218833

• 

  Western blot validation of overexpression lysate (Cat# LY406819) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218833 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PLEKHH2
• Synonyms: PLEKHH1L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218833 representing NM_172069
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAGCTTTCTGAGCCAGAGGGACCAGTAGATTGGAAGGAACGATGTGTAGCTCTGGAGTCCCAAC
  TCATGAAATTTAGAGTTCAAGCAAGCAAGATACGAGAGCTTTTAGCAGAGAAGATGCAACAGCTTGAGAG
  ACAAGTTATTGATGCTGAACGTCAAGCAGAAAAAGCTTTTCAACAGGTACAAGTTATGGAAGATAAATTA
  AAAGCAGCTAATATTCAAACCAGTGAATCAGAGACAAGATTATATAATAAGTGTCAAGATCTGGAGTCGC
  TAATACAGGAAAAAGATGACGTCATTCAAAACTTGGAATTGCAACTTGAAGAGCAGAAACAAATAAGAAT
  ACAAGAAGCTAAAATAATAGAAGAGAAAGCAGCTAAGATAAAAGAATGGGTAACAGTTAAGTTAAATGAG
  CTGGAATTGGAGAATCAGAATCTTCGTTTGATCAACCAAAACCAAACTGAAGAGATAAGAACAATGCAGT
  CAAAACTACAAGAAGTTCAAGGAAAGAAGTCATCCACTGTCTCTACACTAAAGCTTTCGGAAGGCCAGCG
  CCTGAGCAGTTTGACCTTTGGGTGCTTTTTATCTCGAGCAAGGAGTCCTCCTCAAGTAGTAAAATCTGAG
  GAAATGAGCAAGATATCATCGAAAGAACCTGAGTTCACTGAAGGAAAAGACATGGAAGAAATGGAAATTC
  CAGAAAAGTCTGTTGATAACCAAGTTCTAGAAAACAACAGAGGCCAGAGAACATTGCATCAAACCCCTTG
  TGGCTCAGAACAGAATCGGAAAACAAGAACAAGCTTTGCCACAGATGGTGGCATCTCCCAGAATTCTGGG
  GCTCCTGTGAGTGACTGGAGCTCTGATGAGGAAGACGGGAGCAAAGGAAGATCCAAGTCCAGATGCACAT
  CCACCCTCTCCAGTCACACATCTGAGGAAGGGGTCCAGTGTAGCAGGATGGGAAGTGAAATGTATCTGAC
  AGCATCTGATGACAGCAGCTCTATATTTGAGGAAGAGACTTTTGGCATAAAGAGACCAGAACACAAGAAG
  CTATATTCTTGGCAGCAGGAGGCACAGTGGAAAGCTCTAAATAGTCCTCTTGGAAAGGGAAATTCTGAAT
  TAAGTAAAAAGGAACAAGATAGTTCCTCGGATGAACTGAATAAAAAATTTCAATCCCAGAGACTCGATTA
  TTCATCTTCATCGAGTGAAGCCAACACCCCAAGCCCTATTTTGACCCCAGCTTTAATGCCAAAGCATCCT
  AACTCACTCTCTGGAAAAGGAACACAATTAGTGCCTTCATCACACCTGCCACCCCCAAAGTTAAGGATTC
  CTAATGTTTTCAGTATAAGTGCAGCACTAGCCAAAAGGCACTTAAGCCAGCCACAGTTAAGCTCTGACAG
  GATGTTTGGTACAAATAGAAACGCTATAAGCATGATACGACCACTGAGACCTCAGGAAACTGATCTTGAT
  CTAGTTGATGGAGACAGTACAGAAGTTTTAGAGAATATGGACACGAGTTGTGATGATGGATTATTTTCCT
  ATGACTCCTTGGACTCTCCAAATTCAGATGACCAGGAACACTGTGACTCAGCAAAGAAGGTGGCATACAG
  CAAACCTCCAACTCCTCCCCTGCACCGTTTTCCTTCTTGGGAAAGCAGAATTTATGCTGTAGCCAAATCA
  GGTATTCGAATGTCTGAGGCCTTCAATATGGAGAGTGTTAATAAAAATTCTGCTGCAACCCTTTCCTATA
  CTACATCAGGACTTTATACATCTCTGATATACAAGAACATGACCACCCCAGTGTATACAACTTTGAAGGG
  GAAGGCGACCCAAATAAGTAGCAGCCCTTTCCTGGATGACTCATCTGGGTCAGAGGAAGAAGACAGCTCC
  AGATCCAGCTCCCGGACGTCAGAGTCAGACTCACGCAGTAGGAGTGGGCCAGGCAGCCCCAGAGCCATGA
  AACGAGGTGTGTCTCTCTCCTCTGTGGCTTCTGAAAGTGATTATGCTATTCCTCCTGATGCTTACTCCAC
  AGACACGGAGTACTCACAGCCAGAGCAGAAGCTCCCAAAAACTTGCTCATCTTCCAGTGATAATGGGAAA
  AATGAACCACTGGAAAAATCTGGTTATTTATTAAAAATGAGTGGTAAAGTCAAGTCTTGGAAGCGGCGGT
  GGTTTGTTCTTAAAGGTTGTGAATTACTTTACTACAAATCTCCGAGTGATGTAATTAGAAAACCCCAGGG
  CCATATTGAACTTAGTGCATCCTGTAGTATTTTAAGAGGAGATAACAAACAAACAGTTCAGTTGACCACT
  GAAAAACACACATACTATCTGACTGCAGATTCTCCCAATATATTGGAAGAGTGGATTAAAGTGTTACAGA
  ATGTTCTTCGAGTACAAGCTGCCAACCCACTTTCCCTGCAGCCTGAGGGCAAACCCACCATGAAGGGATT
  GCTCACTAAGGTAAAACATGGATATTCCAAGAGAGTCTGGTGTACACTAATAGGAAAGACATTATATTAT
  TTTCGGAGTCAAGAAGATAAGTTTCCTTTAGGTCAGATCAAACTCTGGGAGGTTAAAGTGGAAGAGGTTG
  ACAGATCTTGTGATTCAGATGAAGATTATGAAGCCAGTGGACGAAGTCTGTTATCCACACATTATACTAT
  CGTTATCCATCCCAAAGACCAAGGTCCAACTTACCTCCTAATTGGATCCAAGCATGAAAAGGACACTTGG
  CTTTATCATCTGACTGTTGCAGCTGGAAGCAACAATGTAAACGTTGGATCTGAATTTGAACAACTGGTTT
  GCAAATTGCTAAATATAGACGGGGAGCCTTCCTCTCAGATATGGAGACACCCCACTTTGTGTCACAGTAA
  AGAAGGAATCATTTCCCCTCTGACAACTCTACCTTCCGAAGCCCTGCAGACAGAAGCTATTAAATTATTT
  AAGACCTGCCAGCTTTTTATAAATGCTGCAGTTGACTCTCCTGCAATTGATTACCACATATCTTTAGCCC
  AGAGTGCTTTGCAAATCTGCCTGACACATCCTGAGCTGCAGAATGAAATTTGCTGTCAGCTTATTAAACA
  GACAAGACGAAGACAGCCACAGAATCAACCAGGACCATTGCAGGGCTGGCAGCTCTTGGCACTCTGCGTT
  GGGCTCTTCCTTCCCCATCATCCTTTCCTGTGGCTCCTCAGGCTTCACCTAAAGAGGAATGCAGATTCCA
  GGACAGAATTTGGAAAATATGCCATTTACTGCCAGCGTTGTGTAGAAAGAACGCAACAAAATGGTGACAG
  AGAAGCAAGACCCTCAAGGATGGAAATTCTTTCAACTCTTCTCCGAAACCCTTATCACCATTCTTTGCCC
  TTTAGTATACCTGTGCACTTCATGAATGGGATATACCAGGTAGTTGGTTTTGACGCATCTACCACAGTGG
  AAGAATTTTTGAATACTTTGAACCAGGACACAGGAATGAGGAAACCAGCGCAGTCTGGATTTGCGTTGTT
  CACTGACGATCCTTCTGGCAGAGATTTAGAGCATTGTCTTCAAGGAAACATCAAGATTTGTGACATTATT
  TCCAAATGGGAACAGGCTTCCAAAGAACAGCAGCCTGGAAAATGTGAAGGTACAAGGACTGTTCGTCTGA
  CATACAAAAACAGACTATATTTCTCAGTGCAAGCTCGTGGAGAGACTGATAGAGAAAAGTTGCTGTTAAT
  GTATCAGACAAATGATCAAATCATAAATGGACTTTTTCCTCTGAACAAAGATCTGGCATTAGAAATGGCA
  GCTCTTTTATCTCAGGTAGAGATTGGAGATTTTGAAAGACCTTTCTCAACTCCAGCAGGGCATGTTACCA
  ATCAGTGCAAAGTGAATCAAACTCTAAAGCAAGTCATAGAGAAATTTTATCCTAAAAGGTATAGAGATGG
  CTGTTCTGAAGAGCAGTTAAGGCAGCTTTGCCAGCGACTTTCAACCAGATGGATGGCCCTCCGGGGACAC
  AGTGCTGCTGACTGTGTGCGCATTTATTTGACAGTAGCCAGGAAGTGGCCATTCTTTGGTGCCAAGTTGT
  TTCTTGCAAAACCCATAACTCCATCATCACTTGGAAGTACTTTCTTGTGGCTGGCTGTACATGAGGATGG
  TTTAAGCCTCTTAGAATACAACTCCATGAGGTTAATAGTCAGCTATGTGTACAAGAGTCTAATGACCTTT
  GGAGGCTATCAAGATGATTTTATGGTAGTCATTAACAATACACATTCAAAGGACAAACCAACAGAGAAAT
  TACTTTTTGCCATGGCAAAACCCAAGATTCTTGAAATCACTCTTTTGATCGCCAGTTACATAAACAACTT
  CCATCAGCAAAAGGCAGCATTTCACCACCTCTCTGCTCCAGCACTGCTCTCAGCCCAGACCCGGGGACCC
  CAAGCCAGAATGATGGGAAGCCAGCCTCTTCTGTCAAGCAGCAGACCGACCAAAGGCCCCACCTTACTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218833 representing NM_172069
  Red=Cloning site Green=Tags(s)
  
  MAELSEPEGPVDWKERCVALESQLMKFRVQASKIRELLAEKMQQLERQVIDAERQAEKAFQQVQVMEDKL
  KAANIQTSESETRLYNKCQDLESLIQEKDDVIQNLELQLEEQKQIRIQEAKIIEEKAAKIKEWVTVKLNE
  LELENQNLRLINQNQTEEIRTMQSKLQEVQGKKSSTVSTLKLSEGQRLSSLTFGCFLSRARSPPQVVKSE
  EMSKISSKEPEFTEGKDMEEMEIPEKSVDNQVLENNRGQRTLHQTPCGSEQNRKTRTSFATDGGISQNSG
  APVSDWSSDEEDGSKGRSKSRCTSTLSSHTSEEGVQCSRMGSEMYLTASDDSSSIFEEETFGIKRPEHKK
  LYSWQQEAQWKALNSPLGKGNSELSKKEQDSSSDELNKKFQSQRLDYSSSSSEANTPSPILTPALMPKHP
  NSLSGKGTQLVPSSHLPPPKLRIPNVFSISAALAKRHLSQPQLSSDRMFGTNRNAISMIRPLRPQETDLD
  LVDGDSTEVLENMDTSCDDGLFSYDSLDSPNSDDQEHCDSAKKVAYSKPPTPPLHRFPSWESRIYAVAKS
  GIRMSEAFNMESVNKNSAATLSYTTSGLYTSLIYKNMTTPVYTTLKGKATQISSSPFLDDSSGSEEEDSS
  RSSSRTSESDSRSRSGPGSPRAMKRGVSLSSVASESDYAIPPDAYSTDTEYSQPEQKLPKTCSSSSDNGK
  NEPLEKSGYLLKMSGKVKSWKRRWFVLKGCELLYYKSPSDVIRKPQGHIELSASCSILRGDNKQTVQLTT
  EKHTYYLTADSPNILEEWIKVLQNVLRVQAANPLSLQPEGKPTMKGLLTKVKHGYSKRVWCTLIGKTLYY
  FRSQEDKFPLGQIKLWEVKVEEVDRSCDSDEDYEASGRSLLSTHYTIVIHPKDQGPTYLLIGSKHEKDTW
  LYHLTVAAGSNNVNVGSEFEQLVCKLLNIDGEPSSQIWRHPTLCHSKEGIISPLTTLPSEALQTEAIKLF
  KTCQLFINAAVDSPAIDYHISLAQSALQICLTHPELQNEICCQLIKQTRRRQPQNQPGPLQGWQLLALCV
  GLFLPHHPFLWLLRLHLKRNADSRTEFGKYAIYCQRCVERTQQNGDREARPSRMEILSTLLRNPYHHSLP
  FSIPVHFMNGIYQVVGFDASTTVEEFLNTLNQDTGMRKPAQSGFALFTDDPSGRDLEHCLQGNIKICDII
  SKWEQASKEQQPGKCEGTRTVRLTYKNRLYFSVQARGETDREKLLLMYQTNDQIINGLFPLNKDLALEMA
  ALLSQVEIGDFERPFSTPAGHVTNQCKVNQTLKQVIEKFYPKRYRDGCSEEQLRQLCQRLSTRWMALRGH
  SAADCVRIYLTVARKWPFFGAKLFLAKPITPSSLGSTFLWLAVHEDGLSLLEYNSMRLIVSYVYKSLMTF
  GGYQDDFMVVINNTHSKDKPTEKLLFAMAKPKILEITLLIASYINNFHQQKAAFHHLSAPALLSAQTRGP
  QARMMGSQPLLSSSRPTKGPTLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172069
• ORF Size: 4479 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172069.2
• RefSeq Size: 7028 bp
• RefSeq ORF: 4482 bp
• Locus ID: 130271
• UniProt ID: Q8IVE3
• Cytogenetics: 2p21
• MW: 168 kDa
• Gene Summary: In the kidney glomerulus may play a role in linking podocyte foot processes to the glomerular basement membrane. May be involved in stabilization of F-actin by attenuating its depolymerization. Can recruit TGFB1I1 from focal adhesions to podocyte lamellipodia.[UniProtKB/Swiss-Prot Function]