Cell adhesion molecule 2 (CADM2) (NM_153184) Human Tagged ORF Clone

CAT#: RC218787

CADM2 (Myc-DDK-tagged)-Human cell adhesion molecule 2 (CADM2), transcript variant 3

Product Images

Circular map for RC218787

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cell adhesion molecule 2
• Synonyms: IGSF4D; Necl-3; NECL3; SynCAM 2; synCAM2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218787 representing NM_153184
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTGTTCTCTTCTTGTGCAACCTTTCCTTGGTACCAGCGGCCGCTTCAAAGAATAAAGTTAAAGGCA
  GCCAAGGGCAGTTTCCACTAACACAGAATGTAACCGTTGTTGAAGGTGGAACTGCAATTTTGACCTGCAG
  GGTTGATCAAAATGATAACACCTCCCTCCAGTGGTCAAATCCAGCTCAACAGACTCTGTACTTTGACGAC
  AAGAAAGCTTTAAGGGACAATAGGATCGAGCTGGTTCGCGCTTCCTGGCATGAATTGAGTATTAGTGTCA
  GTGATGTGTCTCTCTCTGATGAAGGACAGTACACCTGTTCTTTATTTACAATGCCTGTCAAAACTTCCAA
  GGCATATCTCACCGTTCTGGGTGTTCCTGAAAAGCCTCAGATTAGTGGATTCTCATCACCAGTTATGGAG
  GGTGACTTGATGCAGCTGACTTGCAAAACATCTGGTAGTAAACCTGCAGCTGATATAAGATGGTTCAAAA
  ATGACAAAGAGATTAAAGATGTAAAATATTTAAAAGAAGAGGATGCAAATCGCAAGACATTCACTGTCAG
  CAGCACACTGGACTTCCGAGTGGACCGGAGTGATGATGGAGTGGCGGTCATCTGCAGAGTAGATCACGAA
  TCCCTCAATGCCACCCCTCAGGTAGCCATGCAGGTGCTAGAAATACACTATACACCATCAGTTAAGATTA
  TACCATCGACTCCTTTTCCACAAGAAGGACAGCCTTTAATTTTGACTTGTGAATCCAAAGGAAAACCACT
  GCCAGAACCTGTTTTGTGGACAAAGGATGGCGGAGAATTACCAGATCCTGACCGAATGGTTGTGAGTGGT
  AGGGAGCTAAACATTCTTTTCCTGAACAAAACGGATAATGGTACATATCGATGTGAAGCCACAAACACCA
  TTGGCCAAAGCAGTGCGGAATATGTTCTCATTGTGCATGATGTTCCCAACACTTTGCTTCCCACTACTAT
  CATCCCCTCCCTTACCACTGCAACAGTCACAACCACTGTAGCCATAACAACCAGCCCAACCACATCTGCA
  ACAACCAGCAGCATCAGAGATCCTAATGCTTTGGCTGGCCAGAATGGCCCTGACCATGCTCTCATAGGAG
  GAATAGTGGCTGTAGTTGTATTTGTCACGCTGTGTTCTATCTTTCTGCTTGGTCGATATCTGGCAAGGCA
  TAAAGGAACGTATTTAACAAATGAAGCTAAAGGAGCTGAAGATGCACCAGATGCTGATACAGCCATTATC
  AATGCTGAAGGCAGCCAAGTCAATGCTGAAGAGAAAAAAGAGTATTTCATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218787 representing NM_153184
  Red=Cloning site Green=Tags(s)
  
  MFVLFLCNLSLVPAAASKNKVKGSQGQFPLTQNVTVVEGGTAILTCRVDQNDNTSLQWSNPAQQTLYFDD
  KKALRDNRIELVRASWHELSISVSDVSLSDEGQYTCSLFTMPVKTSKAYLTVLGVPEKPQISGFSSPVME
  GDLMQLTCKTSGSKPAADIRWFKNDKEIKDVKYLKEEDANRKTFTVSSTLDFRVDRSDDGVAVICRVDHE
  SLNATPQVAMQVLEIHYTPSVKIIPSTPFPQEGQPLILTCESKGKPLPEPVLWTKDGGELPDPDRMVVSG
  RELNILFLNKTDNGTYRCEATNTIGQSSAEYVLIVHDVPNTLLPTTIIPSLTTATVTTTVAITTSPTTSA
  TTSSIRDPNALAGQNGPDHALIGGIVAVVVFVTLCSIFLLGRYLARHKGTYLTNEAKGAEDAPDADTAII
  NAEGSQVNAEEKKEYFI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153184
• ORF Size: 1311 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153184.2, NP_694854.2
• RefSeq Size: 3347 bp
• RefSeq ORF: 1314 bp
• Locus ID: 253559
• UniProt ID: Q8N3J6
• Cytogenetics: 3p12.1
• Domains: ig, IGc2, IG
• Protein Families: Druggable Genome, Transmembrane
• MW: 47.4 kDa
• Gene Summary: This gene encodes a member of the synaptic cell adhesion molecule 1 (SynCAM) family which belongs to the immunoglobulin (Ig) superfamily. The encoded protein has three Ig-like domains and a cytosolic protein 4.1 binding site near the C-terminus. Proteins belonging to the protein 4.1 family crosslink spectrin and interact with other cytoskeletal proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]