INSYN2A (NM_001039762) Human Tagged ORF Clone

CAT#: RC218717

FAM196A (Myc-DDK-tagged)-Human family with sequence similarity 196, member A (FAM196A)

Product Images

Circular map for RC218717

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  Western blot validation of overexpression lysate (Cat# LY421821) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218717 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified INSYN2A protein (Cat# TP318717). The protein was produced from HEK293T cells transfected with INSYN2A cDNA clone (Cat# RC218717) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: INSYN2A
• Synonyms: C10orf141; FAM196A; INSYN2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218717 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCAGTAAGGACACCGGCAAATGCATACTCACAACATCGGAGAGTGAAGTGGAACCCGCCGCCTGCC
  TGGCCCTGGAGATGAAATACGCCCTGGACCCCAACCGGCAGATTAAAAAACGGAACAAAGCCCTGCAGGT
  GCGGTTTAAGGATATCTGCGAGGCACAGAATGAGCAGAGGGACACACAGCTGTCCTCGGGCCAGCTGGGG
  GAGAAGCGGGAGGCCAAGCCCGTGTCCTGCAGAGCAGCCTACCGCAAATACATGACAGTGCCCGCACGCA
  GGTCCATCCCCAACGTCACCAAGAGCACAGGCGTGCAGACCTCGCCCGACCTTAAGAAGTGTTACCAGAC
  GTTCCCTCTGGACCGCAAAAAGGGGAACCTCAAAAGCCTCCCAGCTGCAGATCCCTTTAAAAGCCAAAAC
  AATGGGTTTCTAACAGATGCGAAAGAGAAGAACGAGGCTGGACCCATGGAGGAGGCCCGGCCATGTGGCG
  CGGGGCGGGTGCACAAGACCACAGCCTTGGTTTTCCATTCCAACCAACACATGAACACAGTGGACCAGCC
  TTTGGGGGTCAACTGCACAGAGCCCTGTAAAAGCCCGGAGCCGCTCAGCTATGGAGAAGCTGCGCTCCAA
  AACTCCACTCGGCCTCCATCCGAAGAGCCCGATTACCAGCTGCTCGGGAGGGCCAAGCAGGACCGGGGGA
  GGCCAAACTCCGAGGAGCCCGCTCCACCTGCCCTCAGGAGGGTGTTTAAAACGGAGGTTGCCACCGTTTA
  CGCACCTGCCCTCAGTGCCAGGGCCCCCGAGCCTGGTTTGTCAGACTCTGCAGCCGCCAGCCAGTGGTCA
  CTCTGCCCGGCAGATGACGAGCGGAGGAGAGCCACACATCTCAACGGGCTCCAGGCGCCCTCGGAAACTG
  CCCTGGCCTGCTCACCCCCGATGCAGTGCCTGTCCCCCGAATGTAGTGAGCAGCCGTCGCAGACTCACAC
  CCCGCCGGGGCTGGGGAACCAGCCTAGTCCCACAGCGGTTGCTGCAGGTGAAGAATGCCAACGAATCGTG
  CCTCATACGGAAGTGGTCGACCTCAAAGCACAACTTCAGATGATGGAGAACTTGATCAGTTCAAGCCAAG
  AAACCATCAAAGTGCTCTTGGGGGTCATTCAGGAGTTGGAAAAAGGAGAGGCCCATCGGGAAGGGCTTTC
  ATATCGGACGGGGCAAGACACAGCTAATTGTGACACATGCAGGAACAGTGCATGTATTATCTATAGTGTG
  GAGCTGGATTTTAAGCAGCAAGAAGACAAACTCCAGCCGGTTCTAAGAAAACTCCACCCTATTGAGGAAA
  CTCAGGTCATACCTTCGCCTTACTCTCAGGAGACTTACTCCTCAACTCCCAAGCAAAAATCCAAAACTGA
  ATCTAAAAAGCACGGAAGATGGAAACTCTGGTTCCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218717 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVSKDTGKCILTTSESEVEPAACLALEMKYALDPNRQIKKRNKALQVRFKDICEAQNEQRDTQLSSGQLG
  EKREAKPVSCRAAYRKYMTVPARRSIPNVTKSTGVQTSPDLKKCYQTFPLDRKKGNLKSLPAADPFKSQN
  NGFLTDAKEKNEAGPMEEARPCGAGRVHKTTALVFHSNQHMNTVDQPLGVNCTEPCKSPEPLSYGEAALQ
  NSTRPPSEEPDYQLLGRAKQDRGRPNSEEPAPPALRRVFKTEVATVYAPALSARAPEPGLSDSAAASQWS
  LCPADDERRRATHLNGLQAPSETALACSPPMQCLSPECSEQPSQTHTPPGLGNQPSPTAVAAGEECQRIV
  PHTEVVDLKAQLQMMENLISSSQETIKVLLGVIQELEKGEAHREGLSYRTGQDTANCDTCRNSACIIYSV
  ELDFKQQEDKLQPVLRKLHPIEETQVIPSPYSQETYSSTPKQKSKTESKKHGRWKLWFL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001039762
• ORF Size: 1437 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039762.1, NP_001034851.1
• RefSeq Size: 4407 bp
• RefSeq ORF: 1440 bp
• Locus ID: 642938
• UniProt ID: Q6ZSG2
• Cytogenetics: 10q26.2
• MW: 52.9 kDa
• Gene Summary: Component of the protein machinery at the inhibitory synapses, probably acting as a scaffold. Inhibitory synapses dampen neuronal activity through postsynaptic hyperpolarization. This synaptic inhibition is fundamental for the functioning of the central nervous system, shaping and orchestrating the flow of information through neuronal networks to generate a precise neural code.[UniProtKB/Swiss-Prot Function]