Proprotein convertase PC4 (PCSK4) (NM_017573) Human Tagged ORF Clone

CAT#: RC218596

PCSK4 (Myc-DDK-tagged)-Human proprotein convertase subtilisin/kexin type 4 (PCSK4)

Product Images

Circular map for RC218596

• 

  Western blot validation of overexpression lysate (Cat# LY413713) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218596 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Proprotein convertase PC4
• Synonyms: PC4; SPC5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218596 representing NM_017573
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGCCCGCCCCGATTGCGCTGTGGCTGCGCCTGGTCTTGGCCCTGGCCCTTGTCCGCCCCCGGGCTG
  TGGGGTGGGCCCCGGTCCGAGCCCCCATCTATGTCAGCAGCTGGGCCGTCCAGGTGTCCCAGGGTAACCG
  GGAGGTCGAGCGCCTGGCACGCAAATTCGGCTTCGTCAACCTGGGGCCGATCTTCCCTGACGGGCAGTAC
  TTTCACCTGCGGCACCGGGGCGTGGTCCAGCAGTCCCTGACCCCGCACTGGGGCCACCGCCTGCACCTGA
  AGAAAAACCCCAAGGTGCAGTGGTTCCAGCAGCAGACGCTGCAGCGGCGGGTGAAACGCTCTGTCGTGGT
  GCCCACGGACCCCTGGTTCTCCAAGCAGTGGTACATGAACAGCGAGGCCCAACCAGACCTGAGCATCCTG
  CAGGCCTGGAGTCAGGGGCTGTCAGGCCAGGGCATCGTGGTCTCTGTGCTGGACGATGGCATCGAGAAGG
  ACCACCCGGACCTCTGGGCCAACTACGACCCCCTGGCCAGCTATGACTTCAATGACTACGACCCGGACCC
  CCAGCCCCGCTACACCCCCAGCAAAGAGAACCGGCACGGGACCCGCTGTGCTGGGGAGGTGGCCGCGATG
  GCCAACAATGGCTTCTGTGGTGTGGGGGTCGCTTTCAACGCCCGAATCGGAGGCGTACGGATGCTGGACG
  GTACCATCACCGATGTCATCGAGGCCCAGTCGCTGAGCCTGCAGCCGCAGCACATCCACATTTACAGCGC
  CAGCTGGGGTCCCGAGGACGACGGCCGCACGGTGGACGGCCCCGGCATCCTCACCCGCGAGGCCTTCCGG
  CGTGGTGTGACCAAGGGCCGCGGCGGGCTGGGCACGCTCTTCATCTGGGCCTCGGGCAACGGCGGCCTGC
  ACTACGACAACTGCAACTGCGACGGCTACACCAACAGCATCCACACGCTTTCCGTGGGCAGCACCACCCA
  GCAGGGCCGCGTGCCCTGGTACAGCGAAGCCTGCGCCTCCACCCTCACCACCACCTACAGCAGCGGCGTG
  GCCACCGACCCCCAGATCGTCACCACGGACCTGCATCACGGGTGCACAGACCAGCACACGGGCACCTCGG
  CCTCAGCCCCACTGGCGGCCGGCATGATCGCCCTAGCGCTGGAGGCCAACCCGTTCCTGACGTGGAGAGA
  CATGCAGCACCTGGTGGTCCGCGCGTCCAAGCCGGCGCACCTGCAGGCCGAGGACTGGAGGACCAACGGC
  GTGGGGCGCCAAGTGAGCCATCACTACGGATACGGGCTGCTGGACGCCGGGCTGCTGGTGGACACCGCCC
  GCACCTGGCTGCCCACCCAGCCGCAGAGGAAGTGCGCCGTCCGGGTCCAGAGCCGCCCCACCCCCATCCT
  GCCGCTGATCTACATCAGGGAAAACGTATCGGCCTGCGCCGGCCTCCACAACTCCATCCGCTCGCTGGAG
  CACGTGCAGGCGCAGCTGACGCTGTCCTACAGCCGGCGCGGAGACCTGGAGATCTCGCTCACCAGCCCCA
  TGGGCACGCGCTCCACACTCGTGGCCATACGACCCTTGGACGTCAGCACTGAAGGCTACAACAACTGGGT
  CTTCATGTCCACCCACTTCTGGGATGAGAACCCACAGGGCGTGTGGACCCTGGGCCTAGAGAACAAGGGC
  TACTATTTCAACACGGGGACGTTGTACCGCTACACGCTGCTGCTCTATGGGACGGCCGAGGACATGACAG
  CGCGGCCTACAGGCCCCCAGGTGACCAGCAGCGCGTGTGTGCAGCGGGACACAGAGGGGCTGTGCCAGGC
  GTGTGACGGCCCCGCCTACATCCTGGGACAGCTCTGCCTGGCCTACTGCCCCCCGCGGTTCTTCAACCAC
  ACAAGGCTGGTGACCGCTGGGCCTGGGCACACGGCGGCGCCCGCGCTGAGGGTCTGCTCCAGCTGCCATG
  CCTCCTGCTACACCTGCCGCGGCGGCTCCCCGAGGGACTGCACCTCCTGTCCCCCATCCTCCACGCTGGA
  CCAGCAGCAGGGCTCCTGCATGGGACCCACCACCCCCGACAGCCGCCCCCGGCTTAGAGCTGCCGCCTGT
  CCCCACCACCGCTGCCCAGCCTCGGCCATGGTGCTGAGCCTCCTGGCCGTGACCCTCGGAGGCCCCGTCC
  TCTGCGGCATGTCCATGGACCTCCCACTATACGCCTGGCTCTCCCGTGCCAGGGCCACCCCCACCAAACC
  CCAGGTCTGGCTGCCAGCTGGAACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218596 representing NM_017573
  Red=Cloning site Green=Tags(s)
  
  MRPAPIALWLRLVLALALVRPRAVGWAPVRAPIYVSSWAVQVSQGNREVERLARKFGFVNLGPIFPDGQY
  FHLRHRGVVQQSLTPHWGHRLHLKKNPKVQWFQQQTLQRRVKRSVVVPTDPWFSKQWYMNSEAQPDLSIL
  QAWSQGLSGQGIVVSVLDDGIEKDHPDLWANYDPLASYDFNDYDPDPQPRYTPSKENRHGTRCAGEVAAM
  ANNGFCGVGVAFNARIGGVRMLDGTITDVIEAQSLSLQPQHIHIYSASWGPEDDGRTVDGPGILTREAFR
  RGVTKGRGGLGTLFIWASGNGGLHYDNCNCDGYTNSIHTLSVGSTTQQGRVPWYSEACASTLTTTYSSGV
  ATDPQIVTTDLHHGCTDQHTGTSASAPLAAGMIALALEANPFLTWRDMQHLVVRASKPAHLQAEDWRTNG
  VGRQVSHHYGYGLLDAGLLVDTARTWLPTQPQRKCAVRVQSRPTPILPLIYIRENVSACAGLHNSIRSLE
  HVQAQLTLSYSRRGDLEISLTSPMGTRSTLVAIRPLDVSTEGYNNWVFMSTHFWDENPQGVWTLGLENKG
  YYFNTGTLYRYTLLLYGTAEDMTARPTGPQVTSSACVQRDTEGLCQACDGPAYILGQLCLAYCPPRFFNH
  TRLVTAGPGHTAAPALRVCSSCHASCYTCRGGSPRDCTSCPPSSTLDQQQGSCMGPTTPDSRPRLRAAAC
  PHHRCPASAMVLSLLAVTLGGPVLCGMSMDLPLYAWLSRARATPTKPQVWLPAGT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017573
• ORF Size: 2265 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017573.4, NP_060043.2
• RefSeq Size: 2674 bp
• RefSeq ORF: 2268 bp
• Locus ID: 54760
• UniProt ID: Q6UW60
• Cytogenetics: 19p13.3
• Protein Families: Druggable Genome, Protease
• MW: 82.6 kDa
• Gene Summary: This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an initial autocatalytic processing event in the ER to generate a heterodimer which exits the ER and sorts to subcellular compartments where a second autocatalytic even takes place and the catalytic activity is acquired. This gene encodes one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. The protease is expressed only in the testis, placenta, and ovary. It plays a critical role in fertilization, fetoplacental growth, and embryonic development and processes multiple prohormones including pro-pituitary adenylate cyclase-activating protein and pro-insulin-like growth factor II. [provided by RefSeq, Jan 2014]