CCDC108 (CFAP65) (NM_194302) Human Tagged ORF Clone

CAT#: RC218466

CCDC108 (Myc-DDK-tagged)-Human coiled-coil domain containing 108 (CCDC108), transcript variant 1

Product Images

Circular map for RC218466

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CCDC108
• Synonyms: CCDC108; SPGF40
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218466 representing NM_194302
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTACCTTAACCGGTTGTAGACTGGTGGAGAAAACCCAGAAGGTAGAGAATCCATCAGTCTCATTTG
  CCTCTTCTTTCCCCCTTATTCCTCTTCTCCTAAGAGGCAAGAGTGTTCAGAAGAAACAAGCAGAGAGTAA
  AAGCCAGATAAAGCTCCATACTCAGAGTGCTCCCTTTGGACTGTGTCCCAAGGACATGATGCTCACCCAG
  GCTCCAAGCTCCGTCGTGAGGTCCAGGAACAGCAGGAACCACACCGTGAACTCTGGTGGATCCTGCCTGA
  GTGCCAGCACAGTGGCCATCCCTGCCATCAACGACAGCAGTGCAGCCATGAGTGCCTGCAGCACCATCAG
  CGCCCAGCCCGCAAGCTCCATGGACACTCAGATGCACTCCCCAAAGAAGCAGGAGAGAGTGAACAAGAGG
  GTCATCTGGGGCATTGAGGTGGCTGAGGAGCTGCATTGGAAAGGCTGGGAGCTAGGAAAGGAGACCACAA
  GGAATCTGGTTCTGAAAAATCGATCCTTGAAACTCCAGAAGATGAAGTACAGGCCCCCCAAGACCAAGTT
  CTTCTTCACGGTCATCCCTCAGCCCATCTTCCTGAGCCCAGGCATAACCCTCACGCTCCCCATCGTCTTC
  CGGCCTCTGGAGGCGAAGGAGTACATGGACCAGCTGTGGTTTGAGAAAGCGGAGGGGATGTTCTGTGTCG
  GCCTACGGGCCACCCTGCCCTGCCACAGGCTGATCTGCCGCCCACCATCCCTGCAGCTGCCCATGTGTGC
  TGTGGGAGATACGACTGAGGCCTTTTTCTGCCTGGATAATGTGGGGGACCTGCCCACCTTCTTCACCTGG
  GAGTTCTCCAGCCCATTCCAGATGCTGCCCGCCACGGGGCTCCTGGAGCCAGGCCAGGCCTCTCAGATCA
  AGGTGACCTTTCAGCCCCTTACAGCCGTCATCTACGAGGTGCAGGCCACGTGCTGGTACGGGGCGGGCAG
  CCGGCAGAGGAGCAGCATCCAGCTGCAGGCTGTGGCCAAGTGCGCCCAGCTGCTGGTGAGCATAAAGCAC
  AAGTGCCCGGAGGACCAGGATGCCGAGGGCTTCCAGAAGCTGTTGTACTTTGGCTCTGTTGCTGTGGGCT
  GCACCTCGGAGAGGCAGATCAGGCTACACAACCCGTCGGCGGTAAATGCCCCCTTCAGGATTGAAATTTC
  CCCGGATGAACTGGCCGAAGACCAGGCCTTCTCATGCCCCACGGCCCATGGCATCGTGCTTCCGGGAGAG
  AAGAAATGTGTGTCGGTGTTCTTCCACCCCAAGACTCTGGACACCAGAACTGTGGACTACTGCTCCATCA
  TGCCTTCTGGCTGTGCCTCCAAGACCCTGCTTAAAGTCGTTGGTTTCTGTAGAGGCCCTGCTGTGTCCCT
  GCAGCACTACTGTGTCAACTTCAGCTGGGTCAACCTTGGGGAGCGCTCCGAGCAGCCCCTGTGGATTGAG
  AACCAATCGGACTGCACGGCCCACTTCCAGTTTGCCATCGACTGCTTGGAGAGTGTCTTTACCATCAGGC
  CTGCCTTTGGGACGCTGGTGGGCAAGGCCCGTATGACCCTGCACTGTGCCTTCCAGCCCACTCACCCCAT
  CATCTGCTTTCGGCGTGTGGCCTGTCTCATCCACCACCAGGACCCACTGTTCCTGGACCTGATGGGGACC
  TGCCACTCGGACAGCACCAAGCCAGCCATCCTGAAGCCTCAGCACCTCACCTGGTACCGCACACACCTGG
  CCCGGGGCCTGACGCTCTACCCCCCTGACATCCTGGATGCCATGCTGAAGGAGAAGAAGCTGGCACAGGA
  CCAGAACGGGGCTCTCATGATTCCCATCCAGGATCTGGAGGACATGCCGGCCCCGCAGTACCCTTATATC
  CCCCCCATGACCGAGTTCTTCTTCGACGGCACCAGCGACATAACCATCTTCCCCCCGCCCATCAGTGTAG
  AGCCTGTCGAGGTAGACTTCGGTGCCTGCCCAGGGCCTGAGGCCCCCAACCCTGTACCCCTGTGCCTGAT
  GAACCACACCAAGGGCAAGATCATGGTGGTCTGGACGCGAAGGTCTGACTGCCCCTTCTGGGTGACTCCA
  GAGAGCTGCGACGTGCCCCCACTCAAGTCCATGGCCATGCGCCTGCACTTCCAGCCGCCTCACCCCAACT
  GCCTTTACACGGTGGAGCTCGAAGCCTTCGCCATCTATAAGGTCCTGCAGAGCTACAGTAATATTGAGGA
  GGACTGCACCATGTGCCCATCCTGGTGCCTGACGGTGCGGGCACGAGGCCACAGCTATTTCGCTGGCTTT
  GAGCACCACATCCCCCAGTATTCCCTAGATGTCCCCAAGCTATTTCCAGCAGTGTCCTCCGGTGAGCCCA
  CCTACCGCAGCCTGCTCCTGGTCAACAAAGACTGCAAGCTGCTGACCTTCAGCCTGGCCCCCCAGAGAGG
  CTCAGACGTCATCCTTCGGCCCACTTCGGGCCTTGTGGCACCCGGGGCCCACCAGATCATCCTCATCTGC
  ACCTACCCTGAGGGCAGCTCCTGGAAGCAGCACACTTTCTATCTGCAGTGCAATGCTTCCCCCCAGTATC
  TCAAGGAGGTGAGCATGTACAGCCGGGAGGAGCCACTGCAGCTGAAGCTGGACACCCACAAAAGCCTCTA
  CTTCAAGCCCACCTGGGTGGGCTGCTCCTCCACCAGCCCCTTCACCTTCCGCAACCCCTCGCGTCTGCCC
  CTGCAGTTCGAGTGGAGGGTCTCTGAGCAGCATCGAAAGCTGCTGGCTGTCCAGCCCTCCAGGGGGCTAA
  TCCAGCCCAACGAGAGACTTACGCTGACGTGGACCTTCAGCCCTTTGGAGGAGACCAAGTACCTGTTCCA
  AGTGGGGATGTGGGTCTGGGAAGCCGGCCTGTCCCCAAATGCCAACCCCGCTGCCACCACCCACTACATG
  CTCCGGCTGGTGGGCGTTGGGCTCACCAGCAGCCTCTCTGCAAAGGAAAAGGAGCTGGCCTTTGGGAATG
  TGCTGGTGAACAGCAAGCAGTCCAGGTTCCTTGTCCTCCTGAATGACGGCAACTGCACCCTCTATTACCG
  CCTCTACCTGGAGCAGGGCAGCCCTGAGGCCGTTGACAACCACCCCCTCGCTCTGCAGCTGGACCGAACA
  GAGGGGAGCATGCCACCCCGGTCCCAGGACACCATCTGCCTGACTGCCTGTCCCAAGCAGCGGTCCCAGT
  ACTCCTGGACCATCACCTACTCTCTCCTTTCCCACAGAGATAACAAGGCTGGGGAGAAGCAGGAGCTGTG
  CTGCGTCTCCCTGGTGGCCGTGTACCCCTTGCTTTCCATCCTGGATGTCAGCTCCATGGGCAGTGCTGAG
  GGTATCACCCGGAAGCACCTGTGGCGCCTCTTCTCTCTGGACCTGCTTAACAGTTACTTGGAGCGTGACC
  CCACCCCCTGTGAGCTCACCTACAAGGTGCCCACCCGGCACAGCATGAGCCAGATCCCCCCCGTCCTCAC
  CCCTTTAAGGCTTGACTTCAATTTCGGGGCCGCACCATTCAAGGCCCCACCTTCCGTGGTATTCCTGGCC
  CTGAAGAACAGCGGAGTGGTGTCCCTGGACTGGGCCTTCCTCCTTCCAAGTGACCAGCGGATTGACGTGG
  AGCTCTGGGCAGAGCAAGCAGAGTTGAATTCCACTGAGCTCCACCAGATGCGCGTGCAGGACAATTGCCT
  CTTCTCCATCAGCCCCAAGGCTGGGAGCCTGAGTCCTGGGCAGGAGCAGATGGTGGAGTTAAAATACAGC
  CACCTGTTCATCGGTACTGATCACCTCCCAGTGCTCTTCAAGGTGTCCCATGGCCGGGAGATCCTGCTAA
  ATTTCATAGGTGTGACAGTGAAGCCGGAGCAGAAGTATGTGCACTTCACCTCTACTACCCACCAGTTCAT
  CCCCATTCCCATTGGTGACACGCTACCCCCACGGCAGATTTATGAGCTGTATAATGGTGGCTCAGTGCCC
  GTGACATATGAGGTCCAGACCGATGTCCTGTCACAGGTTCAGGAAAAAAATTTTGATCACCCCATCTTTT
  GCTGCCTCAACCCCAAAGGGGAGATCCAGCCAGGCAGCACTGCCCGGGTCTTGTGGATCTTCTCACCTAT
  CGAGGCCAAGACCTACACGGTGGACGTGCCCATACACATCCTGGGATGGAACTCGGCCCTCATCCACTTC
  CAGGGAGTGGGCTACAACCCCCATATGATGGGGGACACAGCCCCATTCCACAACATCTCCTCGTGGGACA
  ACAGTTCCATACACTCTAGGCTGGTGGTGCCTGGACAGAATGTCTTCCTGTCCCAGTCTCATATTTCCCT
  GGGAAACATACCTGTGCAGAGCAAGTGCAGCCGCCTGCTCTTCCTCAACAACATCTCCAAGAACGAGGAA
  ATTGCCTTCTCCTGGCAGCCAAGTCCTCTAGATTTTGGGGAGGTGTCTGTGAGTCCCATGATAGGGGTGG
  TGGCTCCTGAAGAGACGGTCCCATTTGTGGTGACCTTGAGGGCCTCTGTGCATGCCAGCTTCTACAGTGC
  AGACCTGGTATGCAAGCTGTACTCGCAGCAGCTCATGAGGCAGTATCACAAGGAGCTGCAGGAGTGGAAG
  GACGAGAAGGTGCGGCAGGAAGTGGAGTTCACCATCACCGACATGAAAGTGAAGAAGAGAACATGCTGCA
  CAGCCTGTGAACCTGCGAGGAAGTACAAGACACTGCCTCCCATCAAGAACCAGCAGTCTGTCAGCCGGCC
  TGCCAGCTGGAAACTGCAGACCCCAAAGGAGGAGGTGTCCTGGCCCTGCCCCCAGCCACCCTCGCCAGGC
  ATGCTCTGCCTGGGCCTTACTGCCCGAGCCCATGCCACCGACTACTTTCTGGCTAACTTCTTCTCAGAGT
  TTCCCTGCCACTTTTTGCACCGGGAGCTGCCAAAGAGGAAGGCCCCCAGGGAAGAGTCAGAGACTTCTGA
  GGAAAAATCCCCTAACAAGTGGGGCCCTGTTTCCAAGCAGAAGAAGCAGCTCCTGGTTGACATTCTCACC
  ACAATAATCAGGGGCCTGCTGGAAGACAAGAACTTCCATGAGGCTGTGGACCAAAGCCTGGTGGAGCAGG
  TGCCGTACTTCCGCCAATTCTGGAATGAGCAGTCAACTAAGTTCATGGACCAGAAAAACAGCCTGTACTT
  AATGCCAATCCTGCCTGTACCCTCCAGCAGCTGGGAGGATGGGAAGGGCAAGCAGCCGAAGGAAGACAGA
  CCAGAGCACTATCCAGGGTTGGGAAAGAAGGAAGAGGGGGAGGAGGAGAAGGGTGAAGAGGAAGAAGAAG
  AGTTGGAGGAGGAAGAGGAGGAAGAAGAGGAGACAGAAGAGGAGGAGTTGGGCAAGGAGGAGATAGAGGA
  GAAGGAGGAGGAGAGGGATGAGAAGGAAGAGAAAGTGAGCTGGGCGGGCATCGGGCCCACACCACAGCCT
  GAGTCCCAGGAGTCCATGCAATGGCAGTGGCAACAGCAGCTGAATGTCATGGTGAAGGAGGAGCAAGAAC
  AGGACGAGAAGGAGGCCATCAGAAGGCTCCCGGCCTTCGCCAACCTGCAGGAGGCGCTGCTGGAGAACAT
  GATCCAGAACATCCTGGTGGAGGCGAGCCGCGGGGAGGTGGTACTCACCTCGCGGCCACGCGTCATCGCC
  CTGCCGCCGTTCTGCGTGCCCAGGAGTCTGACCCCGGACACGCTGCTGCCGACGCAGCAAGCAGAGGTAC
  TCCACCCGGTGGTGCCACTTCCTACCGACCTTCCG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218466 representing NM_194302
  Red=Cloning site Green=Tags(s)
  
  MFTLTGCRLVEKTQKVENPSVSFASSFPLIPLLLRGKSVQKKQAESKSQIKLHTQSAPFGLCPKDMMLTQ
  APSSVVRSRNSRNHTVNSGGSCLSASTVAIPAINDSSAAMSACSTISAQPASSMDTQMHSPKKQERVNKR
  VIWGIEVAEELHWKGWELGKETTRNLVLKNRSLKLQKMKYRPPKTKFFFTVIPQPIFLSPGITLTLPIVF
  RPLEAKEYMDQLWFEKAEGMFCVGLRATLPCHRLICRPPSLQLPMCAVGDTTEAFFCLDNVGDLPTFFTW
  EFSSPFQMLPATGLLEPGQASQIKVTFQPLTAVIYEVQATCWYGAGSRQRSSIQLQAVAKCAQLLVSIKH
  KCPEDQDAEGFQKLLYFGSVAVGCTSERQIRLHNPSAVNAPFRIEISPDELAEDQAFSCPTAHGIVLPGE
  KKCVSVFFHPKTLDTRTVDYCSIMPSGCASKTLLKVVGFCRGPAVSLQHYCVNFSWVNLGERSEQPLWIE
  NQSDCTAHFQFAIDCLESVFTIRPAFGTLVGKARMTLHCAFQPTHPIICFRRVACLIHHQDPLFLDLMGT
  CHSDSTKPAILKPQHLTWYRTHLARGLTLYPPDILDAMLKEKKLAQDQNGALMIPIQDLEDMPAPQYPYI
  PPMTEFFFDGTSDITIFPPPISVEPVEVDFGACPGPEAPNPVPLCLMNHTKGKIMVVWTRRSDCPFWVTP
  ESCDVPPLKSMAMRLHFQPPHPNCLYTVELEAFAIYKVLQSYSNIEEDCTMCPSWCLTVRARGHSYFAGF
  EHHIPQYSLDVPKLFPAVSSGEPTYRSLLLVNKDCKLLTFSLAPQRGSDVILRPTSGLVAPGAHQIILIC
  TYPEGSSWKQHTFYLQCNASPQYLKEVSMYSREEPLQLKLDTHKSLYFKPTWVGCSSTSPFTFRNPSRLP
  LQFEWRVSEQHRKLLAVQPSRGLIQPNERLTLTWTFSPLEETKYLFQVGMWVWEAGLSPNANPAATTHYM
  LRLVGVGLTSSLSAKEKELAFGNVLVNSKQSRFLVLLNDGNCTLYYRLYLEQGSPEAVDNHPLALQLDRT
  EGSMPPRSQDTICLTACPKQRSQYSWTITYSLLSHRDNKAGEKQELCCVSLVAVYPLLSILDVSSMGSAE
  GITRKHLWRLFSLDLLNSYLERDPTPCELTYKVPTRHSMSQIPPVLTPLRLDFNFGAAPFKAPPSVVFLA
  LKNSGVVSLDWAFLLPSDQRIDVELWAEQAELNSTELHQMRVQDNCLFSISPKAGSLSPGQEQMVELKYS
  HLFIGTDHLPVLFKVSHGREILLNFIGVTVKPEQKYVHFTSTTHQFIPIPIGDTLPPRQIYELYNGGSVP
  VTYEVQTDVLSQVQEKNFDHPIFCCLNPKGEIQPGSTARVLWIFSPIEAKTYTVDVPIHILGWNSALIHF
  QGVGYNPHMMGDTAPFHNISSWDNSSIHSRLVVPGQNVFLSQSHISLGNIPVQSKCSRLLFLNNISKNEE
  IAFSWQPSPLDFGEVSVSPMIGVVAPEETVPFVVTLRASVHASFYSADLVCKLYSQQLMRQYHKELQEWK
  DEKVRQEVEFTITDMKVKKRTCCTACEPARKYKTLPPIKNQQSVSRPASWKLQTPKEEVSWPCPQPPSPG
  MLCLGLTARAHATDYFLANFFSEFPCHFLHRELPKRKAPREESETSEEKSPNKWGPVSKQKKQLLVDILT
  TIIRGLLEDKNFHEAVDQSLVEQVPYFRQFWNEQSTKFMDQKNSLYLMPILPVPSSSWEDGKGKQPKEDR
  PEHYPGLGKKEEGEEEKGEEEEEELEEEEEEEEETEEEELGKEEIEEKEEERDEKEEKVSWAGIGPTPQP
  ESQESMQWQWQQQLNVMVKEEQEQDEKEAIRRLPAFANLQEALLENMIQNILVEASRGEVVLTSRPRVIA
  LPPFCVPRSLTPDTLLPTQQAEVLHPVVPLPTDLP
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_194302
• ORF Size: 5775 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_194302.2, NP_919278.2
• RefSeq Size: 5975 bp
• RefSeq ORF: 5778 bp
• Locus ID: 255101
• UniProt ID: Q6ZU64
• Cytogenetics: 2q35
• Protein Families: Transmembrane
• MW: 217.1 kDa
• Gene Summary: The protein encoded by this gene has putative coiled-coil domains and may be a transmembrane protein. The chicken ortholog of this gene is involved in the Rose-comb mutation, which is a large chromosome inversion, resulting in altered comb morphology and defects in sperm motility. [provided by RefSeq, Aug 2016]