Myosin 1G (MYO1G) (NM_033054) Human Tagged ORF Clone

CAT#: RC218428

MYO1G (Myc-DDK-tagged)-Human myosin IG (MYO1G)

Product Images

Circular map for RC218428

• 

  Western blot validation of overexpression lysate (Cat# LY409764) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218428 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Myosin 1G
• Synonyms: HA2; HLA-HA2; MHAG
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218428 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGACGAGGAAGGCCCTGAGTATGGCAAACCTGACTTTGTGCTTTTGGACCAAGTGACCATGGAGG
  ACTTCATGAGGAACCTGCAGCTCAGGTTCGAGAAGGGCCGCATCTACACCTACATCGGTGAGGTGCTGGT
  GTCCGTGAACCCCTACCAGGAGCTGCCCCTGTATGGGCCTGAGGCCATCGCCAGGTACCAGGGCCGTGAG
  CTCTATGAGCGGCCACCCCATCTCTATGCTGTGGCCAACGCCGCCTACAAGGCAATGAAGCACCGGTCCA
  GGGACACCTGCATCGTCATCTCAGGGGAGAGTGGGGCAGGGAAGACAGAAGCCAGTAAGCACATCATGCA
  GTACATCGCTGCTGTCACCAATCCAAGCCAGAGGGCTGAGGTGGAGAGGGTCAAGGACGTGCTGCTCAAG
  TCCACCTGTGTGCTGGAGGCCTTTGGCAATGCCCGCACCAACCGCAATCACAACTCCAGCCGCTTTGGCA
  AGTACATGGACATCAACTTTGACTTCAAGGGGGACCCGATCGGAGGACACATCCACAGCTACCTACTGGA
  GAAGTCTCGGGTCCTCAAGCAGCACGTGGGTGAAAGAAACTTCCACGCCTTCTACCAATTGCTGAGAGGC
  AGTGAGGACAAGCAGCTGCATGAACTGCACTTGGAGAGAAACCCTGCTGTATACAATTTCACACACCAGG
  GAGCAGGACTCAACATGACTGTGCACAGTGCCTTGGACAGTGATGAGCAGAGCCACCAGGCAGTGACCGA
  GGCCATGAGGGTCATCGGCTTCAGTCCTGAAGAGGTGGAGTCTGTGCATCGCATCCTGGCTGCCATATTG
  CACCTGGGAAACATCGAGTTTGTGGAGACGGAGGAGGGTGGGCTGCAGAAGGAGGGCCTGGCAGTGGCCG
  AGGAGGCACTGGTGGACCATGTGGCTGAGCTGACGGCCACACCCCGGGACCTCGTGCTCCGCTCCCTGCT
  GGCTCGCACAGTTGCCTCGGGAGGCAGGGAACTCATAGAGAAGGGCCACACTGCAGCTGAGGCCAGCTAT
  GCCCGGGATGCCTGTGCCAAGGCAGTGTACCAGCGGCTGTTTGAGTGGGTGGTGAACAGGATCAACAGTG
  TCATGGAACCCCGGGGCCGGGATCCTCGGCGTGATGGCAAGGACACAGTCATTGGCGTGCTGGACATCTA
  TGGCTTCGAGGTGTTTCCCGTCAACAGTTTCGAGCAGTTCTGCATCAACTACTGCAATGAGAAGCTGCAG
  CAGCTATTCATCCAGCTCATCCTGAAGCAGGAACAGGAAGAGTACGAGCGCGAGGGCATCACCTGGCAGA
  GCGTTGAGTATTTCAACAACGCCACCATTGTGGATCTGGTGGAGCGGCCCCACCGTGGCATCCTGGCCGT
  GCTGGACGAGGCCTGCAGCTCTGCTGGCACCATCACTGACCGAATCTTCCTGCAGACCCTGGACACGCAC
  CACCGCCATCATCTACACTACACCAGCCGCCAGCTCTGCCCCACAGACAAGACCATGGAGTTTGGCCGAG
  ACTTCCGGATCAAGCACTATGCAGGGGACGTCACGTACTCCGTGGAAGGCTTCATCGACAAGAACAGAGA
  TTTCCTCTTCCAGGACTTCAAGCGGCTGCTGTACAACAGCACGGACCCCACTCTACGGGCCATGTGGCCG
  GACGGGCAGCAGGACATCACAGAGGTGACCAAGCGCCCCCTGACGGCTGGCACACTCTTCAAGAACTCCA
  TGGTGGCCCTGGTGGAGAACCTTGCCTCCAAGGAGCCCTTCTACGTCCGCTGCATCAAGCCCAATGAGGA
  CAAGGTAGCTGGGAAGCTGGATGAGAACCACTGTCGCCACCAGGTCGCATACCTGGGGCTGCTGGAGAAT
  GTGAGGGTCCGCAGGGCTGGCTTCGCTTCCCGCCAGCCCTACTCTCGATTCCTGCTCAGGTACAAGATGA
  CCTGTGAATACACATGGCCCAACCACCTGCTGGGCTCCGACAAGGCAGCCGTGAGCGCTCTCCTGGAGCA
  GCATGGGCTGCAGGGGGACGTGGCCTTTGGCCACAGCAAGCTGTTCATCCGCTCACCCCGGACACTGGTC
  ACACTGGAGCAGAGCCGAGCCCGCCTCATCCCCATCATTGTGCTGCTATTGCAGAAGGCATGGCGGGGCA
  CCTTGGCGAGGTGGCGCTGCCGGAGGCTGAGGGCTATCTACACCATCATGCGCTGGTTCCGGAGACACAA
  GGTGCGGGCTCACCTGGCTGAGCTGCAGCGGCGATTCCAGGCTGCAAGGCAGCCGCCACTCTACGGGCGT
  GACCTTGTGTGGCCGCTGCCCCCTGCTGTGCTGCAGCCCTTCCAGGACACCTGCCACGCACTCTTCTGCA
  GGTGGCGGGCCCGGCAGCTGGTGAAGAACATCCCCCCTTCAGACATGCCCCAGATCAAGGCCAAGGTGGC
  CGCCATGGGGGCCCTGCAAGGGCTTCGTCAGGACTGGGGCTGCCGACGGGCCTGGGCCCGAGACTACCTG
  TCCTCTGCCACTGACAATCCCACAGCATCAAGCCTGTTTGCTCAGCGACTAAAGACACTTCGGGACAAAG
  ATGGCTTCGGGGCTGTGCTCTTTTCAAGCCATGTCCGCAAGGTGAACCGCTTCCACAAGATCCGGAACCG
  GGCCCTCCTGCTCACAGACCAGCACCTCTACAAGCTGGACCCTGACCGGCAGTACCGGGTGATGCGGGCC
  GTGCCCCTTGAGGCGGTGACGGGGCTGAGCGTGACCAGCGGAGGAGACCAGCTGGTGGTGCTGCACGCCC
  GCGGCCAGGACGACCTCGTGGTGTGCCTGCACCGCTCCCGGCCGCCATTGGACAACCGCGTTGGGGAGCT
  GGTGGGCGTGCTGGCCGCACACTGCCAGGGGGAGGGCCGCACCCTGGAGGTTCGCGTCTCCGACTGCATC
  CCACTAAGCCATCGCGGGGTCCGGCGCCTCATCTCCGTGGAGCCCAGGCCGGAGCAGCCAGAGCCCGATT
  TCCGCTGCGCTCGCGGCTCCTTCACCCTGCTCTGGCCCAGCCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218428 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEDEEGPEYGKPDFVLLDQVTMEDFMRNLQLRFEKGRIYTYIGEVLVSVNPYQELPLYGPEAIARYQGRE
  LYERPPHLYAVANAAYKAMKHRSRDTCIVISGESGAGKTEASKHIMQYIAAVTNPSQRAEVERVKDVLLK
  STCVLEAFGNARTNRNHNSSRFGKYMDINFDFKGDPIGGHIHSYLLEKSRVLKQHVGERNFHAFYQLLRG
  SEDKQLHELHLERNPAVYNFTHQGAGLNMTVHSALDSDEQSHQAVTEAMRVIGFSPEEVESVHRILAAIL
  HLGNIEFVETEEGGLQKEGLAVAEEALVDHVAELTATPRDLVLRSLLARTVASGGRELIEKGHTAAEASY
  ARDACAKAVYQRLFEWVVNRINSVMEPRGRDPRRDGKDTVIGVLDIYGFEVFPVNSFEQFCINYCNEKLQ
  QLFIQLILKQEQEEYEREGITWQSVEYFNNATIVDLVERPHRGILAVLDEACSSAGTITDRIFLQTLDTH
  HRHHLHYTSRQLCPTDKTMEFGRDFRIKHYAGDVTYSVEGFIDKNRDFLFQDFKRLLYNSTDPTLRAMWP
  DGQQDITEVTKRPLTAGTLFKNSMVALVENLASKEPFYVRCIKPNEDKVAGKLDENHCRHQVAYLGLLEN
  VRVRRAGFASRQPYSRFLLRYKMTCEYTWPNHLLGSDKAAVSALLEQHGLQGDVAFGHSKLFIRSPRTLV
  TLEQSRARLIPIIVLLLQKAWRGTLARWRCRRLRAIYTIMRWFRRHKVRAHLAELQRRFQAARQPPLYGR
  DLVWPLPPAVLQPFQDTCHALFCRWRARQLVKNIPPSDMPQIKAKVAAMGALQGLRQDWGCRRAWARDYL
  SSATDNPTASSLFAQRLKTLRDKDGFGAVLFSSHVRKVNRFHKIRNRALLLTDQHLYKLDPDRQYRVMRA
  VPLEAVTGLSVTSGGDQLVVLHARGQDDLVVCLHRSRPPLDNRVGELVGVLAAHCQGEGRTLEVRVSDCI
  PLSHRGVRRLISVEPRPEQPEPDFRCARGSFTLLWPSR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033054
• ORF Size: 3054 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033054.3
• RefSeq Size: 3313 bp
• RefSeq ORF: 3057 bp
• Locus ID: 64005
• UniProt ID: B0I1T2
• Cytogenetics: 7p13
• MW: 116.4 kDa
• Gene Summary: MYO1G is a plasma membrane-associated class I myosin (see MIM 601478) that is abundant in T and B lymphocytes and mast cells (Pierce et al., 2001 [PubMed 11544309]; Patino-Lopez et al., 2010 [PubMed 20071333]).[supplied by OMIM, Jun 2010]