ING3 (NM_019071) Human Tagged ORF Clone

CAT#: RC218424

ING3 (Myc-DDK-tagged)-Human inhibitor of growth family, member 3 (ING3), transcript variant 1

Product Images

Circular map for RC218424

• 

  Western blot validation of overexpression lysate (Cat# LY412783) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218424 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ING3 protein (Cat# TP318424). The protein was produced from HEK293T cells transfected with ING3 cDNA clone (Cat# RC218424) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ING3
• Synonyms: Eaf4; ING2; MEAF4; p47ING3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218424 representing NM_019071
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGTACCTAGAAGACTATCTGGAAATGATTGAGCAGCTTCCTATGGATCTGCGGGACCGCTTCACGG
  AAATGCGCGAGATGGACCTGCAGGTGCAGAATGCAATGGATCAACTAGAACAAAGAGTCAGTGAATTCTT
  TATGAATGCAAAGAAAAATAAACCTGAGTGGAGGGAAGAGCAAATGGCATCCATCAAAAAAGACTACTAT
  AAAGCTTTGGAAGATGCAGATGAGAAGGTTCAGTTGGCAAACCAGATATATGACTTGGTAGATCGACACT
  TGAGAAAGCTGGATCAGGAACTGGCTAAGTTTAAAATGGAGCTGGAAGCTGATAATGCTGGAATTACAGA
  AATATTAGAGAGGCGATCTTTGGAATTAGACACTCCTTCACAGCCAGTGAACAATCACCATGCTCATTCA
  CATACTCCAGTGGAAAAAAGGAAATATAATCCAACTTCTCACCATACGACAACAGATCATATTCCTGAAA
  AGAAATTTAAATCTGAAGCTCTTCTATCCACCCTTACGTCAGATGCCTCTAAGGAAAATACACTAGGTTG
  TCGAAATAATAATTCCACAGCCTCTTCTAACAATGCCTACAATGTGAATTCCTCCCAACCTCTGGGATCC
  TATAACATTGGCTCGTTATCTTCAGGAACTGGTGCAGGGGCAATTACCATGGCAGCTGCTCAAGCAGTTC
  AGGCTACAGCTCAGATGAAGGAGGGACGAAGAACATCAAGTTTAAAAGCCAGTTATGAAGCATTTAAGAA
  TAATGACTTTCAGTTGGGAAAAGAATTTTCAATGGCCAGGGAAACAGTTGGCTATTCATCATCTTCGGCA
  CTTATGACAACATTAACACAGAATGCCAGTTCATCAGCAGCCGACTCACGGAGTGGTCGAAAGAGCAAAA
  ACAACAACAAGTCTTCAAGCCAGCAGTCATCATCTTCCTCCTCCTCTTCTTCCTTATCATCGTGTTCTTC
  ATCATCAACTGTTGTACAAGAAATCTCTCAACAAACAACTGTAGTGCCAGAATCTGATTCAAATAGTCAG
  GTTGATTGGACTTACGACCCAAATGAACCTCGATACTGCATTTGTAATCAGGTATCTTATGGTGAGATGG
  TGGGATGTGATAACCAAGATTGCCCTATAGAATGGTTCCATTATGGCTGCGTTGGATTGACAGAGGCACC
  AAAAGGCAAATGGTACTGTCCACAGTGCACTGCTGCAATGAAGAGAAGAGGCAGCAGACACAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218424 representing NM_019071
  Red=Cloning site Green=Tags(s)
  
  MLYLEDYLEMIEQLPMDLRDRFTEMREMDLQVQNAMDQLEQRVSEFFMNAKKNKPEWREEQMASIKKDYY
  KALEDADEKVQLANQIYDLVDRHLRKLDQELAKFKMELEADNAGITEILERRSLELDTPSQPVNNHHAHS
  HTPVEKRKYNPTSHHTTTDHIPEKKFKSEALLSTLTSDASKENTLGCRNNNSTASSNNAYNVNSSQPLGS
  YNIGSLSSGTGAGAITMAAAQAVQATAQMKEGRRTSSLKASYEAFKNNDFQLGKEFSMARETVGYSSSSA
  LMTTLTQNASSSAADSRSGRKSKNNNKSSSQQSSSSSSSSSLSSCSSSSTVVQEISQQTTVVPESDSNSQ
  VDWTYDPNEPRYCICNQVSYGEMVGCDNQDCPIEWFHYGCVGLTEAPKGKWYCPQCTAAMKRRGSRHK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019071
• ORF Size: 1254 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019071.3
• RefSeq Size: 2221 bp
• RefSeq ORF: 1257 bp
• Locus ID: 54556
• UniProt ID: Q9NXR8
• Cytogenetics: 7q31.31
• Domains: PHD
• Protein Families: Druggable Genome, Transcription Factors
• MW: 46.6 kDa
• Gene Summary: The protein encoded by this gene is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This gene can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. Overexpression of this gene has been shown to inhibit cell growth and induce apoptosis. Allelic loss and reduced expression of this gene were detected in head and neck cancers. Two alternatively spliced transcript variants encoding different isoforms have been observed. [provided by RefSeq, Jul 2008]