GATA5 (NM_080473) Human Tagged ORF Clone

CAT#: RC218399

GATA5 (Myc-DDK-tagged)-Human GATA binding protein 5 (GATA5)

Product Images

Circular map for RC218399

• 

  Western blot validation of overexpression lysate (Cat# LY409175) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218399 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GATA5
• Synonyms: bB379O24.1; CHTD5; GATAS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218399 representing NM_080473
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACCAGAGCCTGGCGCTGGCCGCGAGCCCCCGCCAGGCCGCCTACGCCGACTCGGGCTCCTTCCTGC
  ACGCTCCGGGCGCCGGCTCTCCGATGTTTGTGCCGCCGGCGCGCGTCCCCTCGATGCTGTCCTACCTGTC
  CGGGTGTGAGCCGAGCCCGCAGCCCCCCGAGCTCGCTGCGCGCCCCGGCTGGGCGCAGACAGCCACCGCG
  GATTCGTCGGCCTTCGGCCCGGGCAGTCCGCACCCCCCAGCCGCGCACCCGCCCGGGGCCACCGCCTTCC
  CTTTCGCGCACAGCCCCTCGGGGCCCGGCAGCGGCGGCAGCGCGGGGGGCCGAGACGGCAGTGCCTACCA
  GGGCGCGCTGTTGCCTCGAGAACAGTTCGCGGCCCCGCTTGGGCGGCCGGTGGGGACCTCGTACTCCGCC
  ACCTACCCGGCCTACGTGAGCCCCGACGTGGCCCAGTCCTGGACTGCCGGGCCCTTCGATGGCAGCGTCC
  TGCACGGCCTCCCAGGCCGCAGGCCCACCTTCGTGTCCGACTTCTTGGAGGAGTTCCCGGGTGAGGGTCG
  TGAGTGTGTCAACTGCGGGGCCCTGTCCACACCGCTGTGGCGCCGAGATGGCACCGGCCACTACCTGTGC
  AATGCCTGCGGCCTCTACCACAAGATGAATGGCGTCAACCGGCCGCTCGTTCGGCCTCAGAAGCGCCTGT
  CCTCGTCCCGCCGCGCCGGCCTCTGCTGCACCAACTGCCACACGACCAACACCACGCTGTGGCGGCGGAA
  CTCGGAGGGGGAGCCCGTGTGCAATGCCTGCGGCCTCTACATGAAGCTGCACGGGGTGCCGCGGCCTCTG
  GCTATGAAGAAAGAAAGCATCCAGACACGGAAGCGGAAGCCAAAGACCATCGCCAAGGCCAGGGGCTCCT
  CAGGATCCACAAGGAATGCCTCGGCCTCCCCATCTGCTGTCGCCAGCACTGACAGCTCAGCAGCCACTTC
  CAAAGCCAAGCCCAGCCTGGCGTCCCCAGTGTGCCCTGGGCCCAGCATGGCCCCCCAGGCCTCTGGCCAG
  GAGGATGACTCTCTTGCCCCCGGCCACTTGGAGTTCAAGTTCGAGCCTGAGGACTTTGCCTTCCCCTCCA
  CGGCCCCGAGCCCCCAGGCTGGCCTCAGGGGGGCTCTGCGCCAAGAGGCCTGGTGTGCGCTGGCCTTGGC
  C
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218399 representing NM_080473
  Red=Cloning site Green=Tags(s)
  
  MYQSLALAASPRQAAYADSGSFLHAPGAGSPMFVPPARVPSMLSYLSGCEPSPQPPELAARPGWAQTATA
  DSSAFGPGSPHPPAAHPPGATAFPFAHSPSGPGSGGSAGGRDGSAYQGALLPREQFAAPLGRPVGTSYSA
  TYPAYVSPDVAQSWTAGPFDGSVLHGLPGRRPTFVSDFLEEFPGEGRECVNCGALSTPLWRRDGTGHYLC
  NACGLYHKMNGVNRPLVRPQKRLSSSRRAGLCCTNCHTTNTTLWRRNSEGEPVCNACGLYMKLHGVPRPL
  AMKKESIQTRKRKPKTIAKARGSSGSTRNASASPSAVASTDSSAATSKAKPSLASPVCPGPSMAPQASGQ
  EDDSLAPGHLEFKFEPEDFAFPSTAPSPQAGLRGALRQEAWCALALA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080473
• ORF Size: 1191 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080473.5
• RefSeq Size: 2630 bp
• RefSeq ORF: 1194 bp
• Locus ID: 140628
• UniProt ID: Q9BWX5
• Cytogenetics: 20q13.33
• Protein Families: Transcription Factors
• MW: 41.1 kDa
• Gene Summary: The protein encoded by this gene is a transcription factor that contains two GATA-type zinc fingers. The encoded protein is known to bind to hepatocyte nuclear factor-1alpha (HNF-1alpha), and this interaction is essential for cooperative activation of the intestinal lactase-phlorizin hydrolase promoter. In other organisms, similar proteins may be involved in the establishment of cardiac smooth muscle cell diversity. [provided by RefSeq, Jul 2008]