SLC22A3 (NM_021977) Human Tagged ORF Clone

CAT#: RC218172

SLC22A3 (Myc-DDK-tagged)-Human solute carrier family 22 (extraneuronal monoamine transporter), member 3 (SLC22A3)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC22A3
• Synonyms: EMT; EMTH; OCT3
• Vector: pCMV6-AC-Myc-DDK
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218172 representing NM_021977
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCTCCTTCGACGAGGCGCTGCAGCGGGTGGGCGAGTTCGGGCGCTTCCAGAGGCGCGTGTTTTTGC
  TGCTGTGCCTGACGGGCGTCACCTTCGCCTTCCTCTTCGTCGGCGTGGTCTTCCTGGGCACGCAGCCCGA
  CCACTACTGGTGCCGCGGGCCAAGTGCCGCGGCGCTGGCCGAGCGCTGCGGCTGGAGCCCGGAGGAGGAG
  TGGAACCGCACGGCGCCCGCCTCCCGCGGCCCAGAGCCCCCCGAGCGCCGCGGCCGCTGCCAGCGCTACC
  TCCTGGAGGCGGCCAACGACAGCGCCTCCGCCACTAGCGCTCTCAGCTGCGCGGACCCACTCGCCGCCTT
  CCCCAACCGTTCGGCTCCCCTTGTGCCGTGCCGCGGCGGCTGGCGCTACGCCCAGGCCCACTCCACCATC
  GTCAGCGAGTTTGACCTTGTCTGTGTCAATGCGTGGATGCTGGACCTCACCCAAGCCATCCTGAACCTCG
  GCTTCCTGACTGGAGCATTCACCTTAGGCTATGCAGCAGACAGGTATGGCAGGATCGTCATTTACTTGCT
  ATCCTGCCTTGGTGTTGGCGTCACTGGGGTTGTGGTGGCCTTTGCACCAAACTTCCCTGTGTTTGTGATC
  TTCCGCTTCCTGCAAGGTGTATTTGGAAAGGGGACGTGGATGACTTGCTACGTGATTGTGACAGAAATAG
  TAGGTTCGAAACAAAGGAGGATTGTGGGAATCGTGATTCAAATGTTCTTTACCCTTGGAATCATAATTCT
  CCCTGGAATTGCCTACTTCATCCCCAACTGGCAAGGAATCCAGTTAGCCATCACGCTGCCCAGCTTTCTC
  TTCCTCCTTTATTACTGGGTGGTCCCTGAGTCTCCCCGTTGGCTGATTACTCGGAAGAAAGGAGATAAAG
  CATTACAGATCCTGAGACGCATTGCTAAGTGCAATGGGAAATACCTCTCATCAAATTACTCAGAGATCAC
  TGTTACAGATGAGGAAGTTAGTAATCCATCCTTTTTAGATCTGGTGAGAACTCCCCAAATGAGGAAATGC
  ACACTTATTCTTATGTTTGCTTGGTTCACAAGCGCAGTGGTGTATCAAGGACTTGTCATGCGCCTGGGAA
  TTATAGGGGGCAACCTCTATATAGACTTTTTCATCTCGGGCGTGGTGGAACTGCCAGGAGCTCTCTTGAT
  CTTACTAACCATTGAGCGCCTTGGACGACGCCTCCCCTTTGCAGCAAGCAATATAGTGGCAGGGGTGGCA
  TGCCTTGTCACTGCGTTCTTACCAGAAGGAATAGCATGGTTGAGGACCACAGTGGCTACATTGGGAAGAC
  TAGGGATAACCATGGCCTTTGAAATTGTTTATTTGGTAAATTCAGAATTGTACCCAACAACATTACGAAA
  TTTCGGAGTTTCGCTCTGTTCAGGTCTGTGTGATTTTGGGGGAATCATAGCCCCATTTCTGCTCTTTCGG
  CTAGCAGCCGTGTGGCTAGAACTACCTCTGATCATCTTTGGTATCCTGGCATCCATCTGTGGTGGCCTTG
  TGATGCTTTTGCCTGAAACCAAGGGTATTGCCTTGCCAGAGACAGTGGATGATGTAGAAAAACTTGGCAG
  TCCACATTCCTGTAAATGTGGCAGGAATAAGAAAACCCCAGTTTCCCGCTCTCACCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218172 representing NM_021977
  Red=Cloning site Green=Tags(s)
  
  MPSFDEALQRVGEFGRFQRRVFLLLCLTGVTFAFLFVGVVFLGTQPDHYWCRGPSAAALAERCGWSPEEE
  WNRTAPASRGPEPPERRGRCQRYLLEAANDSASATSALSCADPLAAFPNRSAPLVPCRGGWRYAQAHSTI
  VSEFDLVCVNAWMLDLTQAILNLGFLTGAFTLGYAADRYGRIVIYLLSCLGVGVTGVVVAFAPNFPVFVI
  FRFLQGVFGKGTWMTCYVIVTEIVGSKQRRIVGIVIQMFFTLGIIILPGIAYFIPNWQGIQLAITLPSFL
  FLLYYWVVPESPRWLITRKKGDKALQILRRIAKCNGKYLSSNYSEITVTDEEVSNPSFLDLVRTPQMRKC
  TLILMFAWFTSAVVYQGLVMRLGIIGGNLYIDFFISGVVELPGALLILLTIERLGRRLPFAASNIVAGVA
  CLVTAFLPEGIAWLRTTVATLGRLGITMAFEIVYLVNSELYPTTLRNFGVSLCSGLCDFGGIIAPFLLFR
  LAAVWLELPLIIFGILASICGGLVMLLPETKGIALPETVDDVEKLGSPHSCKCGRNKKTPVSRSHL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_021977
• ORF Size: 1668 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021977.4
• RefSeq Size: 5624 bp
• RefSeq ORF: 1671 bp
• Locus ID: 6581
• UniProt ID: O75751
• Cytogenetics: 6q25.3
• Domains: sugar_tr
• Protein Families: Transmembrane
• MW: 61.1 kDa
• Gene Summary: Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. This gene is one of three similar cation transporter genes located in a cluster on chromosome 6. The encoded protein contains twelve putative transmembrane domains and is a plasma integral membrane protein. [provided by RefSeq, Jul 2008]