SIRT1 (NM_012238) Human Tagged Lenti ORF Clone

CAT#: RC218134L2

Lenti ORF clone of Human sirtuin 1 (SIRT1), transcript variant 1, mGFP tagged

Product Images

Circular map for RC218134L2

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  Double digestion of RC218134L2 using SgfI and MluI

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: mGFP
• Symbol: SIRT1
• Synonyms: SIR2; SIR2alpha; SIR2L1
• Vector: pLenti-C-mGFP
• E. coli Selection: Chloramphenicol (34 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RC218134).
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012238
• ORF Size: 2241 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012238.3
• RefSeq Size: 4107 bp
• RefSeq ORF: 2244 bp
• Locus ID: 23411
• UniProt ID: Q96EB6
• Cytogenetics: 10q21.3
• Domains: SIR2
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• MW: 81.5 kDa
• Gene Summary: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]