Collagen IX (COL9A1) (NM_001851) Human Tagged ORF Clone

CAT#: RC218130

COL9A1 (Myc-DDK-tagged)-Human collagen, type IX, alpha 1 (COL9A1), transcript variant 1

Product Images

Circular map for RC218130

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  Western blot validation of overexpression lysate (Cat# LY419709) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218130 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified COL9A1 protein (Cat# TP318130). The protein was produced from HEK293T cells transfected with COL9A1 cDNA clone (Cat# RC218130) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Collagen IX
• Synonyms: DJ149L1.1.2; EDM6; MED; STL4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218130 representing NM_001851
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGACCTGCTGGAAAATTCCAGTTTTCTTCTTTGTGTGCAGTTTCCTGGAACCCTGGGCATCTGCAG
  CTGTCAAGCGTCGCCCCAGATTCCCTGTCAATTCCAATTCTAATGGTGGAAATGAACTCTGTCCAAAGAT
  CAGGATTGGCCAAGATGACTTACCAGGGTTTGATCTGATCTCTCAGTTCCAGGTAGATAAAGCAGCATCT
  AGAAGAGCTATCCAGAGAGTAGTGGGATCAGCTACATTGCAGGTGGCTTACAAGTTGGGAAATAATGTAG
  ACTTCAGGATTCCAACTAGGAATTTATATCCCAGTGGACTGCCTGAAGAATACTCCTTCTTGACGACGTT
  TCGAATGACTGGAAGCACTCTCAAAAAGAACTGGAACATTTGGCAGATTCAGGATTCCTCTGGGAAGGAG
  CAAGTTGGCATAAAGATTAATGGCCAAACACAATCTGTTGTATTTTCATACAAGGGACTGGATGGAAGTC
  TCCAAACAGCAGCCTTTTCGAATTTGTCCTCCTTGTTTGATTCCCAGTGGCATAAGATCATGATTGGCGT
  GGAGAGGAGTAGTGCTACTCTTTTTGTTGACTGCAACAGGATTGAATCTTTACCTATAAAGCCAAGAGGC
  CCAATTGACATTGATGGCTTTGCTGTGCTGGGAAAACTTGCAGATAATCCTCAAGTTTCTGTTCCATTTG
  AACTTCAATGGATGCTGATCCATTGTGACCCCCTGCGGCCCAGGAGAGAAACTTGCCATGAGCTGCCAGC
  CAGAATAACGCCCAGCCAGACCACCGACGAGAGAGGTCCCCCGGGTGAGCAGGGTCCTCCCGGGCCTCCG
  GGCCCCCCTGGAGTTCCAGGCATCGATGGCATCGACGGTGACCGAGGTCCTAAGGGCCCCCCGGGCCCCC
  CGGGTCCTGCAGGTGAACCGGGAAAGCCAGGAGCTCCAGGCAAGCCTGGCACACCTGGCGCTGATGGATT
  AACAGGACCTGATGGATCCCCTGGCTCCATTGGGTCAAAGGGACAAAAAGGAGAACCTGGTGTGCCTGGA
  TCGCGTGGATTTCCAGGCCGTGGTATTCCTGGACCCCCTGGTCCTCCTGGGACAGCAGGACTCCCTGGAG
  AGCTTGGCCGTGTAGGACCTGTTGGTGACCCTGGGAGAAGAGGACCACCTGGCCCCCCTGGCCCCCCAGG
  ACCCAGAGGAACAATTGGCTTTCATGATGGAGATCCATTGTGTCCCAATGCCTGTCCACCAGGTCGCTCA
  GGATATCCAGGCCTACCAGGCATGAGGGGTCATAAAGGGGCTAAAGGAGAAATTGGTGAACCAGGAAGAC
  AAGGACACAAGGGTGAAGAAGGTGACCAGGGAGAACTCGGAGAAGTTGGAGCTCAAGGACCTCCAGGAGC
  CCAGGGTTTGCGAGGCATCACCGGCATAGTTGGGGACAAAGGGGAAAAAGGTGCTCGGGGCTTAGATGGT
  GAACCTGGGCCTCAGGGTCTTCCTGGTGCACCTGGTGATCAAGGACAGCGAGGACCTCCAGGAGAAGCAG
  GTCCCAAAGGAGATAGAGGGGCTGAAGGTGCTAGAGGAATTCCTGGTCTCCCTGGGCCCAAAGGAGACAC
  GGGTTTGCCAGGTGTGGATGGCCGTGATGGGATCCCTGGAATGCCTGGAACAAAGGGTGAACCAGGAAAA
  CCTGGGCCTCCTGGTGATGCAGGATTGCAGGGGTTACCAGGTGTACCTGGAATTCCTGGTGCAAAGGGTG
  TTGCTGGTGAAAAGGGTAGCACAGGTGCTCCAGGGAAGCCTGGTCAGATGGGAAATTCAGGCAAACCGGG
  CCAACAGGGGCCTCCAGGAGAGGTGGGACCCCGAGGACCCCAGGGGCTTCCTGGCAGTAGAGGAGAATTA
  GGACCAGTGGGATCCCCAGGCCTACCAGGTAAACTGGGTTCTCTGGGTAGCCCTGGCCTCCCTGGCTTGC
  CTGGGCCCCCTGGACTTCCTGGAATGAAAGGTGACAGGGGTGTAGTCGGTGAACCGGGTCCAAAGGGTGA
  ACAGGGTGCCTCTGGTGAAGAAGGTGAAGCAGGAGAAAGGGGGGAACTTGGAGATATAGGATTACCTGGC
  CCAAAGGGATCTGCAGGTAATCCTGGGGAACCTGGCTTGAGAGGGCCTGAGGGAAGTCGGGGGCTTCCTG
  GAGTGGAAGGACCAAGAGGACCACCTGGACCCCGGGGTGTGCAGGGAGAACAGGGTGCCACCGGCCTGCC
  TGGTGTCCAGGGCCCTCCGGGTAGAGCACCGACAGATCAGCACATTAAGCAGGTTTGCATGAGAGTCATA
  CAAGAACATTTTGCTGAGATGGCTGCCAGTCTTAAGCGTCCAGACTCAGGTGCCACTGGGCTTCCTGGAA
  GGCCTGGCCCTCCTGGTCCCCCCGGCCCTCCTGGAGAGAATGGTTTCCCAGGCCAGATGGGAATTCGTGG
  CCTTCCGGGCATTAAGGGGCCCCCTGGTGCTCTTGGTTTGAGGGGACCTAAAGGTGACTTGGGAGAAAAG
  GGGGAGCGTGGCCCTCCAGGAAGAGGTCCCAACGGTTTGCCTGGAGCTATAGGTCTCCCAGGTGACCCAG
  GCCCTGCCAGCTATGGCAGAAATGGCCGAGACGGTGAGCGAGGCCCCCCAGGGGTGGCAGGAATTCCTGG
  AGTGCCTGGACCCCCGGGACCTCCTGGGCTTCCCGGTTTCTGTGAGCCAGCCTCCTGCACCATGCAGGCT
  GGTCAGCGAGCATTTAACAAAGGGCCTGACCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218130 representing NM_001851
  Red=Cloning site Green=Tags(s)
  
  MKTCWKIPVFFFVCSFLEPWASAAVKRRPRFPVNSNSNGGNELCPKIRIGQDDLPGFDLISQFQVDKAAS
  RRAIQRVVGSATLQVAYKLGNNVDFRIPTRNLYPSGLPEEYSFLTTFRMTGSTLKKNWNIWQIQDSSGKE
  QVGIKINGQTQSVVFSYKGLDGSLQTAAFSNLSSLFDSQWHKIMIGVERSSATLFVDCNRIESLPIKPRG
  PIDIDGFAVLGKLADNPQVSVPFELQWMLIHCDPLRPRRETCHELPARITPSQTTDERGPPGEQGPPGPP
  GPPGVPGIDGIDGDRGPKGPPGPPGPAGEPGKPGAPGKPGTPGADGLTGPDGSPGSIGSKGQKGEPGVPG
  SRGFPGRGIPGPPGPPGTAGLPGELGRVGPVGDPGRRGPPGPPGPPGPRGTIGFHDGDPLCPNACPPGRS
  GYPGLPGMRGHKGAKGEIGEPGRQGHKGEEGDQGELGEVGAQGPPGAQGLRGITGIVGDKGEKGARGLDG
  EPGPQGLPGAPGDQGQRGPPGEAGPKGDRGAEGARGIPGLPGPKGDTGLPGVDGRDGIPGMPGTKGEPGK
  PGPPGDAGLQGLPGVPGIPGAKGVAGEKGSTGAPGKPGQMGNSGKPGQQGPPGEVGPRGPQGLPGSRGEL
  GPVGSPGLPGKLGSLGSPGLPGLPGPPGLPGMKGDRGVVGEPGPKGEQGASGEEGEAGERGELGDIGLPG
  PKGSAGNPGEPGLRGPEGSRGLPGVEGPRGPPGPRGVQGEQGATGLPGVQGPPGRAPTDQHIKQVCMRVI
  QEHFAEMAASLKRPDSGATGLPGRPGPPGPPGPPGENGFPGQMGIRGLPGIKGPPGALGLRGPKGDLGEK
  GERGPPGRGPNGLPGAIGLPGDPGPASYGRNGRDGERGPPGVAGIPGVPGPPGPPGLPGFCEPASCTMQA
  GQRAFNKGPDP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001851
• ORF Size: 2763 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001851.6
• RefSeq Size: 3704 bp
• RefSeq ORF: 2766 bp
• Locus ID: 1297
• UniProt ID: P20849
• Cytogenetics: 6q13
• Protein Families: Druggable Genome
• MW: 91.87 kDa
• Gene Summary: This gene encodes one of the three alpha chains of type IX collagen, which is a minor (5-20%) collagen component of hyaline cartilage. Type IX collagen is usually found in tissues containing type II collagen, a fibrillar collagen. Studies in knockout mice have shown that synthesis of the alpha 1 chain is essential for assembly of type IX collagen molecules, a heterotrimeric molecule, and that lack of type IX collagen is associated with early onset osteoarthritis. Mutations in this gene are associated with osteoarthritis in humans, with multiple epiphyseal dysplasia, 6, a form of chondrodysplasia, and with Stickler syndrome, a disease characterized by ophthalmic, orofacial, articular, and auditory defects. Two transcript variants that encode different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]