PAN3 (NM_175854) Human Tagged ORF Clone

CAT#: RC218027

PAN3 (Myc-DDK-tagged)-Human PAN3 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) (PAN3)

Product Images

Circular map for RC218027

• 

  Coomassie blue staining of purified PAN3 protein (Cat# TP318027). The protein was produced from HEK293T cells transfected with PAN3 cDNA clone (Cat# RC218027) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PAN3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218027 representing NM_175854
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGGAGGTGCTTTAACTGATACAAGTCTCACAGATTCCTATTTTAGCACCAGCTTTATTGGAGTCA
  ATGGATTTGGAAGCCCTGTAGAAACAAAATATCCCCTGATGCAGAGAATGACTAATAGTAGCAGCTCCCC
  AAGCCTTCTAAATGACAGTGCCAAGCCATATTCAGCCCATGATCCTCTAACATCACCTGCTTCATCCTTG
  TTTAATGACTTTGGTGCCCTCAACATCTCTCAGAGACGAAAGACACCAAATCCTACTGCAAGCGAGTTTA
  TTCCTAAAGGAGGATCAACCTCCAGGCTGAGTAACGTGTCCCAGTCAAATATGTCTGCCTTCTCTCAAGT
  TTTCTCTCACCCATCCATGGGAAGCCCTGCTACTGCTGGATTAGCGCCAGGAATGTCGTTGTCTGCTGGG
  TCTTCCCCTCTTCATTCCCCCAAGATTACTCCACATACTTCTCCTGCTCCCAGAAGAAGAAGTCACACTC
  CAAATCCAGCAAGTTACATGGTGCCTTCTAGTGCCTCTACATCTGTTAATAATCCTGTTTCTCAGACTCC
  GTCTTCTGGTCAGGTGATCCAAAAGGAAACTGTTGGTGGGACGACTTACTTCTATACAGACACAACTCCA
  GCACCTTTGACTGGAATGGTGTTTCCAAACTATCATATTTATCCTCCAACTGCACCTCACGTTGCTTATA
  TGCAACCGAAAGCAAACGCACCTTCCTTCTTCATGGCTGATGAACTCCGACAGGAGCTGATCAACAGACA
  TTTAATAACAATGGCTCAAATTGATCAAGCAGATATGCCAGCAGTTCCTACAGAGGTTGACAGCTACCAT
  AGCCTATTCCCTCTAGAACCACTGCCACCTCCCAACCGGATACAGAAATCAAGTAATTTTGGATATATTA
  CATCTTGCTACAAAGCTGTAAACAGCAAAGATGATCTGCCATATTGCCTTCGGAGGATACATGGTTTTCG
  TCTTGTTAACACAAAGTGCATGGTGTTGGTCGACATGTGGAAGAAAATTCAACACTCAAATATCGTAACT
  TTGCGTGAAGTATTTACCACTAAAGCATTTGCTGAGCCCTCTCTTGTGTTTGCATATGATTTCCATGCTG
  GAGGAGAAACTATGATGAGCAGACACTTTAATGACCCTAATGCTGATGCCTACTTCACCAAGAGAAAGTG
  GGGTCAGCACGAGGGACCATTGCCCAGGCAGCATGCTGGATTATTGCCAGAATCTCTTATTTGGGCATAT
  ATTGTCCAACTAAGTTCTGCATTGCGTACCATTCATACAGCAGGTTTGGCATGTCGAGTTATGGATCCAA
  CAAAGATTCTGATAACTGGCAAAACAAGGTTGCGAGTAAATTGTGTTGGAGTTTTTGATGTTTTAACATT
  TGATAACAGTCAAAATAATAATCCATTGGCATTAATGGCCCAGTACCAGCAAGCAGATCTGATATCATTA
  GGAAAAGTTGTGTTGGCTTTGGCTTGCAACTCTTTGGCAGGAATTCAGCGAGAGAATTTACAGAAAGCCA
  TGGAACTGGTGACAATCAACTATTCCTCTGACCTGAAGAATCTGATTTTGTATTTGTTGACTGACCAAAA
  CAGGATGCGAAGTGTAAATGACATCATGCCCATGATTGGTGCTCGATTTTATACTCAATTGGATGCTGCT
  CAAATGAGAAATGATGTCATAGAGGAAGACCTTGCAAAGGAGGTTCAAAATGGAAGACTGTTTAGGCTCC
  TAGCAAAATTGGGAACAATCAATGAGAGGCCGGAGTTTCAGAAGGATCCCACTTGGTCAGAGACTGGAGA
  CCGTTATCTGTTGAAACTCTTTAGGGATCATCTTTTTCATCAGGTGACAGAAGCAGGTGCTCCCTGGATT
  GACCTCAGTCATATAATTTCTTGTCTTAACAAGCTAGATGCTGGTGTGCCAGAAAAAATAAGCCTGATTT
  CCAGAGATGAGAAGAGTGTACTTGTGGTGACCTACAGTGACTTAAAGCGCTGCTTTGAAAATACTTTTCA
  AGAACTGATTGCAGCTGCAAATGGTCAGTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218027 representing NM_175854
  Red=Cloning site Green=Tags(s)
  
  MDGGALTDTSLTDSYFSTSFIGVNGFGSPVETKYPLMQRMTNSSSSPSLLNDSAKPYSAHDPLTSPASSL
  FNDFGALNISQRRKTPNPTASEFIPKGGSTSRLSNVSQSNMSAFSQVFSHPSMGSPATAGLAPGMSLSAG
  SSPLHSPKITPHTSPAPRRRSHTPNPASYMVPSSASTSVNNPVSQTPSSGQVIQKETVGGTTYFYTDTTP
  APLTGMVFPNYHIYPPTAPHVAYMQPKANAPSFFMADELRQELINRHLITMAQIDQADMPAVPTEVDSYH
  SLFPLEPLPPPNRIQKSSNFGYITSCYKAVNSKDDLPYCLRRIHGFRLVNTKCMVLVDMWKKIQHSNIVT
  LREVFTTKAFAEPSLVFAYDFHAGGETMMSRHFNDPNADAYFTKRKWGQHEGPLPRQHAGLLPESLIWAY
  IVQLSSALRTIHTAGLACRVMDPTKILITGKTRLRVNCVGVFDVLTFDNSQNNNPLALMAQYQQADLISL
  GKVVLALACNSLAGIQRENLQKAMELVTINYSSDLKNLILYLLTDQNRMRSVNDIMPMIGARFYTQLDAA
  QMRNDVIEEDLAKEVQNGRLFRLLAKLGTINERPEFQKDPTWSETGDRYLLKLFRDHLFHQVTEAGAPWI
  DLSHIISCLNKLDAGVPEKISLISRDEKSVLVVTYSDLKRCFENTFQELIAAANGQL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175854
• ORF Size: 2061 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175854.5, NP_787050.4
• RefSeq Size: 5643 bp
• RefSeq ORF: 2664 bp
• Locus ID: 255967
• UniProt ID: Q58A45
• Cytogenetics: 13q12.2
• Protein Families: Druggable Genome
• MW: 76 kDa
• Gene Summary: Regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. PAN3 acts as a positive regulator for PAN activity, recruiting the catalytic subunit PAN2 to mRNA via its interaction with RNA and PABP, and to miRNA targets via its interaction with GW182 family proteins.[UniProtKB/Swiss-Prot Function]