ZSCAN2 (NM_181877) Human Tagged ORF Clone

CAT#: RC218021

ZSCAN2 (Myc-DDK-tagged)-Human zinc finger and SCAN domain containing 2 (ZSCAN2), transcript variant 1. Note: ORF is codon optimized

Product Images

Circular map for RC218021

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZSCAN2
• Synonyms: ZFP29; ZNF854
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218021 ORF sequence, codon optimized.
  Due to the complexity of NM_181877, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGCCGCAGATATTCCAAGGGTCACCACACCCCTGTCTAGCTTGGTGCAGGTGCCTCAGGAGGAGG
  ACAGGCAGGAGGAGGAGGTCACAACGATGATACTCGAAGATGACTCCTGGGTGCAGGAGGCCGTCCTTCA
  AGAGGATGGTCCAGAGAGTGAACCATTCCCACAGTCAGCGGGCAAAGGTGGACCCCAAGAGGAAGTGACT
  AGAGGGCCCCAGGGTGCTCTCGGACGCCTCCGAGAGCTGTGCAGGCGGTGGCTGAGGCCCGAGGTGCACA
  CCAAGGAGCAAATGCTCACTATGTTGCCCAAGGAGATCCAAGCATGGCTGCAGGAGCACCGCCCTGAGTC
  TTCTGAGGAAGCTGCCGCTCTGGTTGAAGATCTTACCCAGACCCTGCAGGATAGCGATTTCGAGATTCAG
  TCCGAGAACGGAGAGAATTGTAATCAGGACATGTTTGAGAACGAGTCCCGGAAAATCTTCTCCGAGATGC
  CTGAAGGTGAGTCAGCCCAGCATAGTGACGGAGAGAGCGACTTCGAAAGGGACGCAGGAATCCAGCGCCT
  CCAAGGACACTCCCCAGGCGAAGACCACGGCGAGGTGGTGAGTCAAGACCGAGAAGTGGGCCAGTTGATC
  GGGCTGCAGGGGACATACCTCGGTGAGAAGCCTTATGAATGCCCCCAGTGTGGAAAGACATTCAGCAGGA
  AATCCCATCTGATTACCCATGAGCGGACGCACACGGGAGAGAAATACTATAAGTGTGACGAATGTGGCAA
  GAGTTTTAGCGATGGCAGTAACTTCTCCAGGCACCAGACAACACACACTGGAGAGAAGCCCTACAAGTGT
  CGCGACTGTGGTAAGTCATTTAGCCGCAGCGCCAACCTGATCACGCATCAGCGAATTCATACTGGAGAGA
  AGCCGTTCCAGTGCGCGGAGTGCGGAAAATCCTTTTCACGATCTCCTAATCTGATCGCACACCAGAGGAC
  CCATACAGGTGAAAAGCCTTATTCCTGTCCTGAGTGCGGGAAATCATTTGGGAATCGCAGCTCTCTGAAC
  ACCCACCAAGGCATTCATACCGGGGAGAAGCCATACGAGTGTAAAGAGTGCGGCGAATCATTTTCTTATA
  ATTCCAACCTGATTAGGCACCAGCGAATTCATACAGGGGAAAAGCCTTACAAGTGTACAGACTGTGGGCA
  ACGCTTCTCACAAAGTTCTGCTCTCATCACCCACCGGAGGACTCACACCGGAGAAAAGCCTTACCAGTGC
  AGTGAGTGCGGGAAATCATTTTCAAGATCCAGCAACTTGGCTACCCACAGGCGCACCCATATGGTGGAGA
  AACCATATAAGTGCGGTGTGTGTGGGAAGAGTTTTAGTCAGTCCTCCTCCCTGATTGCACACCAGGGAAT
  GCATACCGGAGAGAAGCCCTACGAGTGCCTGACGTGCGGGGAGAGCTTCAGTTGGAGCTCCAACTTGCTT
  AAACACCAGCGAATTCATACCGGTGAGAAGCCCTATAAATGCTCTGAATGCGGAAAGTGCTTTTCCCAGC
  GCTCACAGCTGGTCGTGCACCAGAGAACTCATACAGGAGAAAAGCCGTACAAGTGCCTCATGTGCGGCAA
  AAGCTTCAGCAGGGGGTCTATACTCGTTATGCATCAGCGGGCTCACTTGGGCGACAAGCCCTACCGGTGC
  CCCGAATGTGGGAAAGGCTTTTCCTGGAATTCAGTTCTGATCATTCACCAGCGGATTCACACTGGTGAAA
  AGCCGTACAAATGTCCTGAGTGCGGGAAAGGGTTTAGTAATAGCAGTAATTTTATCACGCACCAAAGAAC
  TCATATGAAGGAGAAGCTCTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218021 representing NM_181877
  Red=Cloning site Green=Tags(s)
  
  MMAADIPRVTTPLSSLVQVPQEEDRQEEEVTTMILEDDSWVQEAVLQEDGPESEPFPQSAGKGGPQEEVT
  RGPQGALGRLRELCRRWLRPEVHTKEQMLTMLPKEIQAWLQEHRPESSEEAAALVEDLTQTLQDSDFEIQ
  SENGENCNQDMFENESRKIFSEMPEGESAQHSDGESDFERDAGIQRLQGHSPGEDHGEVVSQDREVGQLI
  GLQGTYLGEKPYECPQCGKTFSRKSHLITHERTHTGEKYYKCDECGKSFSDGSNFSRHQTTHTGEKPYKC
  RDCGKSFSRSANLITHQRIHTGEKPFQCAECGKSFSRSPNLIAHQRTHTGEKPYSCPECGKSFGNRSSLN
  THQGIHTGEKPYECKECGESFSYNSNLIRHQRIHTGEKPYKCTDCGQRFSQSSALITHRRTHTGEKPYQC
  SECGKSFSRSSNLATHRRTHMVEKPYKCGVCGKSFSQSSSLIAHQGMHTGEKPYECLTCGESFSWSSNLL
  KHQRIHTGEKPYKCSECGKCFSQRSQLVVHQRTHTGEKPYKCLMCGKSFSRGSILVMHQRAHLGDKPYRC
  PECGKGFSWNSVLIIHQRIHTGEKPYKCPECGKGFSNSSNFITHQRTHMKEKLY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181877
• ORF Size: 1842 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181877.1, NM_181877.2, NM_181877.3, NP_870992.2
• RefSeq Size: 3756 bp
• RefSeq ORF: 1845 bp
• Locus ID: 54993
• UniProt ID: Q7Z7L9
• Cytogenetics: 15q25.2
• Protein Families: Transcription Factors
• MW: 69.5 kDa
• Gene Summary: The protein encoded by this gene contains several copies of zinc finger motif, which is commonly found in transcriptional regulatory proteins. Studies in mice show that this gene is expressed during embryonic development, and specifically in the testis in adult mice, suggesting that it may play a role in regulating genes in germ cells. Alternative splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]